LOCUS       pAAV_SOX9_5             6984 bp ds-DNA     circular     28-NOV-2022
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            Jensen_U9H_1_pAAV_SOX9_5_pLann.gbk Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             join(1..756,6880..6984)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="DQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAE"
     rep_origin      927..1515
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1698..1754
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSLMQL"
     repeat_region   complement(1748..1881)
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.0"
                     /fragment="False"
                     /other="repeat_region"
     rep_origin      complement(1933..1976)
                     /label="SV40 ori (fragment)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="32.4"
                     /fragment="True"
                     /other="rep_origin"
     misc_feature    2073..2962
                     /label="SOX9_C LHA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2963..2989
                     /label="GA linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2996..3316
                     /label="FKBP-FV36"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2996..3316
                     /label="FKBP-FV36"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE"
     misc_feature    3332..4039
                     /label="mNeonGreen"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             3332..4039
                     /label="mNeonGreen"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK"
     misc_feature    4040..4060
                     /label="GA linker"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4061..4102
                     /label="V5"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4103..4966
                     /label="SOX9_C RHA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     repeat_region   5015..5147
                     /label="AAV2 ITR (fragment)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.3"
                     /fragment="True"
                     /other="repeat_region"
     CDS             5142..5211
                     /label="lacZα (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.2"
                     /fragment="True"
                     /other="CDS"
                     /translation="QLA**RRGPHRSPFPTVAQPEWR"
     CDS             5222..5527
                     /label="M13 gene IV (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="23.9"
                     /fragment="True"
                     /other="CDS"
                     /translation="VAMAGGNIVLDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL*"
     rep_origin      5515..5943
                     /label="f1 ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             6033..6436
                     /label="M13 gene II (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="32.8"
                     /fragment="True"
                     /other="CDS"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVETSQK*LPSPA*IYQLERLNIILMVI*LSPAFLTRLNLYLHITQALHLKYMRVLKIFILALK*RLLPQKYYRVIMFLVQPI*LYALRLYCLILLILCLACMIYWM"
     misc_feature    complement(6444..6543)
                     /label="bom (fragment)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(6645..6680)
                     /label="rop (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
                     /translation="LARFGDDGENL*"
     promoter        6775..6879
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
       61 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1741 cattaatgca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
     1801 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
     1861 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
     1921 agccatgctc tagcggcctc ggcctctgca taaataaaaa aaattagtca gccatgagct
     1981 tggcccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     2041 aacattaccg ccatgttgac attgattatt gagcacagcc cttgttgatt ttctcgtgct
     2101 tgttctttta ttgtccacag ggcaatccca gggcccaccg accccaccca ccacccccaa
     2161 aaccgacgtg cagccgggca aggctgacct gaagcgagag gggcgcccct tgccagaggg
     2221 gggcagacag ccccctatcg acttccgcga cgtggacatc ggcgagctga gcagcgacgt
     2281 catctccaac atcgagacct tcgatgtcaa cgagtttgac cagtacctgc cgcccaacgg
     2341 ccacccgggg gtgccggcca cgcacggcca ggtcacctac acgggcagct acggcatcag
     2401 cagcaccgcg gccaccccgg cgagcgcggg ccacgtgtgg atgtccaagc agcaggcgcc
     2461 gccgccaccc ccgcagcagc ccccacaggc cccgccggcc ccgcaggcgc ccccgcagcc
     2521 gcaggcggcg cccccacagc agccggcggc acccccgcag cagccacagg cgcacacgct
     2581 gaccacgctg agcagcgagc cgggccagtc ccagcgaacg cacatcaaga cggagcagct
     2641 gagccccagc cactacagcg agcagcagca gcactcgccc caacagatcg cctacagccc
     2701 cttcaacctc ccacactaca gcccctccta cccgcccatc acccgctcac agtacgacta
     2761 caccgaccac cagaactcca gctcctacta cagccacgcg gcaggccagg gcaccggcct
     2821 ctactccacc ttcacctaca tgaaccccgc tcagcgcccc atgtacaccc ccatcgccga
     2881 cacctctggg gtcccttcca tcccgcagac ccacagcccc cagcactggg aacaacccgt
     2941 ctacacacag ctcactcgac ctgccggtgc aggcgctgga gccggtgcca ccggtggagt
     3001 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg
     3061 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa gttgattcct cccgggacag
     3121 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg
     3181 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta
     3241 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga
     3301 gcttctaaaa ctggaaggca gtggaggatc catggtgagc aagggcgagg aggataacat
     3361 ggcctctctc ccagcgacac atgagttaca catctttggc tccatcaacg gtgtggactt
     3421 tgacatggtg ggtcagggca ccggcaatcc aaatgatggt tatgaggagt taaacctgaa
     3481 gtccaccaag ggtgacctcc agttctcccc ctggattctg gtccctcata tcgggtatgg
     3541 cttccatcag tacctgccct accctgacgg gatgtcgcct ttccaggccg ccatggtaga
     3601 tggctccgga taccaagtcc atcgcacaat gcagtttgaa gatggtgcct cccttactgt
     3661 taactaccgc tacacctacg agggaagcca catcaaagga gaggcccagg tgaaggggac
     3721 tggtttccct gctgacggtc ctgtaatgac caactcgctg accgctgcgg actggtgcag
     3781 gtcgaagaag acttacccca acgacaaaac catcatcagt acctttaagt ggagttacac
     3841 cactggaaat ggcaagcgct accggagcac tgcgcggacc acctacacct ttgccaagcc
     3901 aatggcggct aactatctga agaaccagcc gatgtacgtg ttccgtaaga cggagctcaa
     3961 gcactccaag accgagctca acttcaagga gtggcaaaag gcctttaccg atgtgatggg
     4021 catggacgag ctgtacaagc tcgaggctgg agccggtgcc ggcaagccca tccccaaccc
     4081 cctgctgggc ctggacagca cctgaggagg cctcccacga agggcgaaga tggccgagat
     4141 gatcctaaaa ataaccgaag aaagagagga ccaaccagaa ttccctttgg acatttgtgt
     4201 ttttttgttt ttttattttg ttttgttttt tcttcttctt cttcttcctt aaagacattt
     4261 aagctaaagg caactcgtac ccaaatttcc aagacacaaa catgacctat ccaagcgcat
     4321 tacccacttg tggccaatca gtggccaggc caaccttggc taaatggagc agcgaaatca
     4381 acgagaaact ggacttttta aaccctcttc agagcaagcg tggaggatga tggagaatcg
     4441 tgtgatcagt gtgctaaatc tctctgcctg tttggacttt gtaattattt ttttagcagt
     4501 aattaaagaa aaaagtcctc tgtgaggaat attctctatt ttaaatattt ttagtatgta
     4561 ctgtgtatga ttcattacca ttttgagggg atttatacat atttttagat aaaattaaat
     4621 gctcttattt ttccaacagc taaactactc ttagttgaac agtgtgccct agcttttctt
     4681 gcaaccagag tatttttgta cagatttgct ttctcttaca aaaagaaaaa aaaatcctgt
     4741 tgtattaaca tttaaaaaca gaattgtgtt atgtgatcag ttttgggggt taactttgct
     4801 taattcctca ggctttgcga tttaaggagg agctgcctta aaaaaaaata aaggccttat
     4861 tttgcaatta tgggagtaaa caatagtcta gagaagcatt tggtaagctt tatcatatat
     4921 atatttttta aagaagagaa aaacaccttg agccttaaaa cggtgcagca tggctacgta
     4981 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
     5041 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     5101 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctggcg taatagcgaa
     5161 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgattc
     5221 cgttgcaatg gctggcggta atattgttct ggatattacc agcaaggccg atagtttgag
     5281 ttcttctact caggcaagtg atgttattac taatcaaaga agtattgcga caacggttaa
     5341 tttgcgtgat ggacagactc ttttactcgg tggcctcact gattataaaa acacttctca
     5401 ggattctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg
     5461 ctctgattct aacgaggaaa gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc
     5521 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5581 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5641 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5701 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5761 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5821 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5881 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5941 attttaacaa aatattaacg cttacaattt aaatatttgc ttatacaatc ttcctgtttt
     6001 tggggctttt ctgattatca accggggtac atatgattga catgctagtt ttacgattac
     6061 cgttcatcga ttctcttgtt tgctccagac tctcaggcaa tgacctgata gcctttgtag
     6121 agacctctca aaaatagcta ccctctccgg catgaattta tcagctagaa cggttgaata
     6181 tcatattgat ggtgatttga ctgtctccgg cctttctcac ccgtttgaat ctttacctac
     6241 acattactca ggcattgcat ttaaaatata tgagggttct aaaaattttt atccttgcgt
     6301 tgaaataaag gcttctcccg caaaagtatt acagggtcat aatgtttttg gtacaaccga
     6361 tttagcttta tgctctgagg ctttattgct taattttgct aattctttgc cttgcctgta
     6421 tgatttattg gatgttggaa tcgcctgatg cggtattttc tccttacgca tctgtgcggt
     6481 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6541 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     6601 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6661 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6721 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6961 ctggtgaaag taaaagatgc tgaa
//