LOCUS       pAAV-hSyn-fDIO_mScarlet_GPHN_C4d 7196 bp   circular     28-NOV-2022
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_C4c 7175 bp   circular     03-DEC-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      29-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      23-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      22-OCT-2020
EMBL_ID     pAAV-hSyn-mScarlet_GPHN_P2_XcmI_XhoI 6852 bp   circular 04-MAY-2020
EMBL_ID     pAAV-hSyn-mScarlet_GPHN_P2_XcmI_XhoI 6852 bp   circular 07-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      22-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      28-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_C4a 7187 bp   circular     03-DEC-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_C4d 7196 bp   circular     03-DEC-2020
UNIMARK     pAAV-hSyn-fDIO_mScarlet_GPHN_C4d features
            pAAV-hSyn-fDIO_mScarlet_GPHN_C4d
FEATURES             Location/Qualifiers
     misc_feature    1..7238
                     /comment="Molecule is created with Unipro UGENE v34.0"
                     /ugene_name="source"
     primer_bind     38..59
                     /label="F1ori-F"
                     /note="F1 origin, forward primer"
     primer_bind     complement(213..232)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     332..354
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(392..410)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        478..582
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             583..1443
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     andrelated antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     primer_bind     complement(801..820)
                     /label="Amp-R"
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1614..2202
                     /direction="RIGHT"
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2103..2122
                     /label="pBR322ori-F"
                     /note="pBR322 origin, forward primer"
     repeat_region   2264..2393
                     /label="AAV2 ITR (alternate)"
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        2438..2885
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     regulatory      2899..2908
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiation of translation (Kozak, 1987)"
     misc_feature    2923..2956
                     /ugene_name="FRT_1"
                     /ugene_group="repeat_region"
     primer_bind     complement(3185..3203)
                     /label="mCherry-R"
                     /note="mCherry, reverse primer"
     primer_bind     3453..3472
                     /label="mCherry-F"
                     /note="mCherry, forward primer"
     misc_feature    complement(4396..4458)
                     /ugene_name="C4d"
     misc_feature    complement(5347..6042)
                     /ugene_name="mScarlet"
     misc_feature    6206..6682
                     /ugene_name="polyA_signal"
     misc_feature    6722..6851
                     /ugene_name="repeat_region"
ORIGIN
        1 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA
       61 ACAACACTCA ACTCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG
      121 GTCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA
      181 TTAACGTTTA CAATTTTATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA
      241 AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG
      301 GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA
      361 CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT
      421 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC
      481 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA
      541 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC
      601 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA
      661 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA
      721 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG
      781 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA
      841 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC
      901 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC
      961 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA
     1021 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG
     1081 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA
     1141 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA
     1201 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC
     1261 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA
     1321 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA
     1381 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG
     1441 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
     1501 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
     1561 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
     1621 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
     1681 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
     1741 GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
     1801 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
     1861 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     1921 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
     1981 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
     2041 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
     2101 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
     2161 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     2221 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTCCTGCAG GCAGCTGCGC
     2281 GCTCGCTCGC TCACTGAGGC CGCCCGGGCG TCGGGCGACC TTTGGTCGCC CGGCCTCAGT
     2341 GAGCGAGCGA GCGCGCAGAG AGGGAGTGGC CAACTCCATC ACTAGGGGTT CCTGCGGCCG
     2401 CACGCGTGTG TCTAGACTGC AGAGGGCCCT GCGTATGAGT GCAAGTGGGT TTTAGGACCA
     2461 GGATGAGGCG GGGTGGGGGT GCCTACCTGA CGACCGACCC CGACCCACTG GACAAGCACC
     2521 CAACCCCCAT TCCCCAAATT GCGCATCCCC TATCAGAGAG GGGGAGGGGA AACAGGATGC
     2581 GGCGAGGCGC GTGCGCACTG CCAGCTTCAG CACCGCGGAC AGTGCCTTCG CCCCCGCCTG
     2641 GCGGCGCGCG CCACCGCCGC CTCAGCACTG AAGGCGCGCT GACGTCACTC GCCGGTCCCC
     2701 CGCAAACTCC CCTTCCCGGC CACCTTGGTC GCGTCCGCGC CGCCGCCGGC CCAGCCGGAC
     2761 CGCACCACGC GAGGCGCGAG ATAGGGGGGC ACGGGCGCGA CCATCTGCGC TGCGGCGCCG
     2821 GCGACTCAGC GCTGCCTCAG TCTGCGGTGG GCAGCGGAGG AGTCGTGTCG TGCCTGAGAG
     2881 CGCAGTCGAG AAGGTACCGG ATCCTCTAGA GTCGACTCCG GAGAAGTTCC TATTCTCTAG
     2941 AAAGTATAGG AACTTCGCAG AATGGTAGCT GGATTGTAGC TGCTATTAGC AATATGAAAC
     3001 CTCTTAGAAG TTCCTATTCT TCAAAAGGTA TAGGAACTTC GGCGCGCCTC ATAGCCGTCC
     3061 GATGACCATG ACGTCCACCA CCTCGCCTTT GTGAAGTTCC ACATACTGCT CTGTCTTTGG
     3121 AGGTAGCATC AGCAATCCAT TGGCACTGCG CATGCTCATC AGACGGCTGC TCATCTGATT
     3181 ACCTGTACTC TGGGCCCAGG GCAGTGGTTC TTGGTGATGC CAAGTCAGTA TACACCGGTG
     3241 GTATTCTGGG CGAGGGTCCA GCTTTACGTC ACATGATAAC CTAGCTTTGA TGATGGTTGG
     3301 CCGAGGATCC AAGATACCCT GCATTTTTCT CAGTGCGGGT ACAACAAAGA GGTTGCAGGT
     3361 GACCACAGCT GATACAGGAT TCCCTGGTAG TGCAAAAATA ATTTTTCTTA CACCATCAAT
     3421 ATCCAAAGTT GCAAATGTTG TTGGCAAGCC TGGCTTCATA AAAACCCTGC CAAAATGGAT
     3481 CTGAGCATGA AGATCGATGT CCAGCACCTG CTTGAGATAG TCCTTTTCCC CCATGGATAC
     3541 ACCTCCTGAC GTGATGATGA CATCAGCACG GCTGATACCC TCATTCAAGG CATTGAGTAA
     3601 GTCATCTGGG TTGTCTCCTA CAATTCCCAA GTTGATTGTT GGGTAACCAT GTTCCTGAAT
     3661 TGTTGCTAGA AGTGTTGATC GATTGCTGTC CCGAATCTTT CCTGGTAAGA GGTCATCTTC
     3721 AGGATTTAGT AGCTCATTCC CTGTTGACAT AACGGCAACC ACTGGAAACT TATTAACTTC
     3781 AACTTCTGTG ACACCTACAG TTGCAAGGAG ACCAATTTCT GAAGGGCCCA TATGGGTTCC
     3841 TTTTGCCAAA ACACACTCCC CTCTTTTAAT GTCGTGGCCG ATGGGTCTGA TGTCTTGGCC
     3901 TGGCCGAGCT TGCACCAGAA TTCGCACTTC AAGTTCTTCA GTACCATCAT CTGATTCCCT
     3961 GATGAGTTCA GTATCTTCAA CTTGCACTAC TGCATCAGCA CCGCAGGGGA TTGGAGCACC
     4021 TGTTGTAACC CGCATCACTT GTCCGGGCAT TACTGTCTGA GTTGGCTGTT CACCAGCTTG
     4081 GGATTCCCCA ATGATGAAAC GATCTCCTGG ACCATCAGCA GCTCGAACAG CATAGCCATC
     4141 TTTTACTGAT GCTGGGAATG GGGGTAGGTT GTCTTTTGCA TACACATCTT GAGCAAGGAC
     4201 TCGCCCCATT CCATCTCGGT AATTGATGAT TTCTGTACCA AGCACCGGAG TCATCTCCAG
     4261 AACTGTAATG AAGGCTTTGT CCATAGACGT CAGAGGAAAA GGAGACATGC GATGTCTTCT
     4321 AGCCACCTTG GTGATATCTA CAGCACTGTG ACTGGCTCTT AGAATGTTCT CCTTGCTGCT
     4381 GCACCTGGAC TGGACTCTTA AGTCGTAGCC TCTACTCCTC CAGAGTTCAT CTTTAAGCCC
     4441 TTCCAATCTA GAGTGGAGTT TTGGTGTTGG ACAAGAAGCT GTAGAGAGGC GAGATGTAGC
     4501 CTGGGCACGG GGCGACTCTG AAGGAGTAGT GCTAAGGGAG GCTGTGTCTC GTGGGAGCAC
     4561 CTGAACGCCA CGAGAAATGA TGGAGTCTGG AATCTTTGCA GCAAGAGCTG CTGCAGTAAT
     4621 ATGTGATGAG GAACTATCTT CTGTCGAAGC TACACCACTG TCTTTCTTTT CTTCTTCCTC
     4681 CTCTTCACAC TGAACTCCTT TGTCTTCTGT CTGCTTATGT GGGCTAGTTG TAGGAGGTGG
     4741 AGAAAGAGGA GGTGGTGGGG AAGGTAAATC TTCAAGTTCA TCATGCACCT CCTTTACTTT
     4801 TACAATGGCA TCACGTAAAA GGTCAATGGC ATGAGGTAGA GCTGGCAGTA TGAACTGAAA
     4861 GCATTCCTGA GATCCTTTCT TGCTACCAGG CAGGTTAATT ATCAGAGTTT TCCCTCTTAT
     4921 TCCACACACT GGTCTAGAGA GCATGCCCAG AGGTGTAACA TTAAGTGATC CCATCAGCAT
     4981 TGCCAGGGCC ATCCCTGGCG CTTCCCGTTC TATTACTTCT TTTGTGGCCT CTGGAGTGAC
     5041 ATCTCGTGGT GCAAACCCTG TTCCGCCAGT TGTTAATATC AAATTAAGTT CCTTCTCATC
     5101 ACACCAGTCT ATGAGTGTTT CCTTGATTTC TTCTATTTCA TCTGGTACTA TCTTGTATGC
     5161 TGATATAGTC CCACCCAACA AAGAAGGATC CTGGACGAGA TCTTTTAGAT TTATCCCACT
     5221 GCGGTCTTCT GCAAGATTCC TGAAGCAGCT ATCACTCACT GTGAGGACTC CGACACGGAT
     5281 TTGATGGTCG TGGTTTGTGA GGATCATTCC CTCGGTCGCC ATGCTCGAGA TCTGAGTCCG
     5341 GCCGGACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG CGGCCCTCGG AGCGTTCGTA
     5401 CTGTTCCACC ACGGTGTAGT CCTCGTTGTG GGAGGTGATG TCCAACTTGC GGTCCACGTT
     5461 GTAGGCGCCG GGCATCTGCA CGGGCTTCTT GGCCTTGTAG GTGGTCTTGA AGTCCGCCAG
     5521 GTAGCGCCCG CCGTCCTTCA GGCGCAGGGC CATCTTAATG TCGCCCTTCA GCACGCCGTC
     5581 CTCGGGGTAC AACCGCTCGG TGGACGCTTC CCAGCCCATT GTCTTCTTCT GCATTACGGG
     5641 GCCGTCAGGA GGGAAGTTGG TGCCGCGGAG CTTCACCTTG TAGATCAGGG TGCCGTCCTC
     5701 CAGGGAGGTG TCCTGGGTCA CGGTCACGGC GCCGCCGTCC TCGAAGTTCA TCACGCGCTC
     5761 CCACTTGAAG CCCTCGGGGA AGGACTGCTT ATAGTAGTCG GGGATGTCGG CGGGGTGCTT
     5821 GGTGAAGGCC CTGGAGCCGT ACATGAACTG AGGGGACAGG ATGTCCCAGG AGAAGGGCAG
     5881 GGGGCCACCC TTGGTCACCT TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG GGCGGCCCTC
     5941 GCCCTCGCCC TCGATCTCGA ACTCGTGGCC GTTCATGGAG CCCTCCATGT GCACCTTGAA
     6001 CCGCATGAAC TCCTTGATCA CTGCCTCGCC CTTGCTCACC ATGGTGGCGC TAGCGAAGTT
     6061 CCTATACTTT CTAGAGAATA GGAACTTCTT GCCTTAACCC AGAAATTATC ACTGTTATTC
     6121 TTTAGAATGG TGCAAAGAGA AGTTCCTATA CCTTTTGAAG AATAGGAACT TCGAATTCGA
     6181 TATCAAGCTT ATCGATGAGA TCTACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT
     6241 CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG
     6301 CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG
     6361 GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG
     6421 CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT
     6481 TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA
     6541 ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC
     6601 CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC
     6661 CACTGCTCCC TTCCCTGTCC TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG
     6721 CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG
     6781 GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG
     6841 CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC
     6901 GGTATTTCAC ACCGCATACG TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA
     6961 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCTTAGCGC
     7021 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG
     7081 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA
     7141 AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTT
//
