LOCUS       pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         28-NOV-2022
EMBL_ID     pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         06-APR-2021
EMBL_ID     pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         24-FEB-2021
EMBL_ID     pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         12-FEB-2021
EMBL_ID     pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         06-JAN-2021
EMBL_ID     pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         21-JAN-2021
EMBL_ID     pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         19-FEB-2021
EMBL_ID     pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         04-MAR-2021
EMBL_ID     pAAV_CamKII_moxBFP-IRES_Flpo 7945 bp   circular         15-APR-2021
UNIMARK     pAAV_CamKII_moxBFP-IRES_Flpo annotations
            pAAV_CamKII_moxBFP-IRES_Flpo
FEATURES             Location/Qualifiers
     misc_feature    1..7945
                     /comment="Molecule is created with Unipro UGENE v37.0"
                     /ugene_name="source"
     primer_bind     complement(73..93)
                     /label="WPRE-R"
                     /note="WPRE, reverse primer"
     CDS             complement(491..502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label="Factor Xa site"
                     /translation="IEGR"
     polyA_signal    640..1116
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(839..858)
                     /label="hGH-PA-R"
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   1156..1296
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     repeat_region   1156..1285
                     /label="AAV2 ITR"
     rep_origin      1371..1826
                     /direction="RIGHT"
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1458..1477)
                     /label="F1ori-R"
                     /note="F1 origin, reverse primer"
     primer_bind     1668..1689
                     /label="F1ori-F"
                     /note="F1 origin, forward primer"
     primer_bind     complement(1843..1862)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1962..1984
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2022..2040)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2108..2212
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             2213..3073
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     andrelated antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     primer_bind     complement(2431..2450)
                     /label="Amp-R"
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3244..3832
                     /direction="RIGHT"
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3733..3752
                     /label="pBR322ori-F"
                     /note="pBR322 origin, forward primer"
     repeat_region   3894..4023
                     /label="AAV2 ITR (alternate)"
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    5369..6085
                     /ugene_name="moxBFP"
     misc_feature    6105..6657
                     /ugene_name="IRES"
     misc_feature    6657..7937
                     /ugene_name="Flpo"
     misc_feature    join(7941..7945,1..27)
                     /ugene_name="WPRE_fwd"
ORIGIN
        1 CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG
       61 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA
      121 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT
      181 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT
      241 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGAAC
      301 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG
      361 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC
      421 GTCCTTTCCT TGGCTGCTCG CCTATGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG
      481 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT
      541 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC
      601 CTCCCCGCAT CGATACCGAG CGCTGCTCGA GAGATCTACG GGTGGCATCC CTGTGACCCC
      661 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA
      721 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG
      781 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC
      841 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC
      901 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG
      961 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG
     1021 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA
     1081 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTGTAGGT AACCACGTGC
     1141 GGACCGAGCG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC
     1201 GCTCGCTCAC TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG
     1261 CCTCAGTGAG CGAGCGAGCG CGCAGCTGCC TGCAGGGGCG CCTGATGCGG TATTTTCTCC
     1321 TTACGCATCT GTGCGGTATT TCACACCGCA TACGTCAAAG CAACCATAGT ACGCGCCCTG
     1381 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC
     1441 CAGCGCCTTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG
     1501 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG
     1561 GCACCTCGAC CCCAAAAAAC TTGATTTGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG
     1621 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT
     1681 CCAAACTGGA ACAACACTCA ACTCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT
     1741 GCCGATTTCG GTCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT
     1801 TAACAAAATA TTAACGTTTA CAATTTTATG GTGCACTCTC AGTACAATCT GCTCTGATGC
     1861 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
     1921 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
     1981 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT
     2041 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
     2101 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     2161 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
     2221 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
     2281 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
     2341 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
     2401 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
     2461 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
     2521 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
     2581 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
     2641 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
     2701 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
     2761 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
     2821 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
     2881 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
     2941 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
     3001 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
     3061 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
     3121 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
     3181 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
     3241 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
     3301 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
     3361 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
     3421 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
     3481 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
     3541 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
     3601 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
     3661 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
     3721 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
     3781 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
     3841 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTCCTGCAG
     3901 GCAGCTGCGC GCTCGCTCGC TCACTGAGGC CGCCCGGGCG TCGGGCGACC TTTGGTCGCC
     3961 CGGCCTCAGT GAGCGAGCGA GCGCGCAGAG AGGGAGTGGC CAACTCCATC ACTAGGGGTT
     4021 CCTGCGGCCG CACGCGTTTA ACATTATGGC CTTAGGTCAC TTCATCTCCA TGGGGTTCTT
     4081 CTTCTGATTT TCTAGAAAAT GAGATGGGGG TGCAGAGAGC TTCCTCAGTG ACCTGCCCAG
     4141 GGTCACATCA GAAATGTCAG AGCTAGAACT TGAACTCAGA TTACTAATCT TAAATTCCAT
     4201 GCCTTGGGGG CATGCAAGTA CGATATACAG AAGGAGTGAA CTCATTAGGG CAGATGACCA
     4261 ATGAGTTTAG GAAAGAAGAG TCCAGGGCAG GGTACATCTA CACCACCCGC CCAGCCCTGG
     4321 GTGAGTCCAG CCACGTTCAC CTCATTATAG TTGCCTCTCT CCAGTCCTAC CTTGACGGGA
     4381 AGCACAAGCA GAAACTGGGA CAGGAGCCCC AGGAGACCAA ATCTTCATGG TCCCTCTGGG
     4441 AGGATGGGTG GGGAGAGCTG TGGCAGAGGC CTCAGGAGGG GCCCTGCTGC TCAGTGGTGA
     4501 CAGATAGGGG TGAGAAAGCA GACAGAGTCA TTCCGTCAGC ATTCTGGGTC TGTTTGGTAC
     4561 TTCTTCTCAC GCTAAGGTGG CGGTGTGATA TGCACAATGG CTAAAAAGCA GGGAGAGCTG
     4621 GAAAGAAACA AGGACAGAGA CAGAGGCCAA GTCAACCAGA CCAATTCCCA GAGGAAGCAA
     4681 AGAAACCATT ACAGAGACTA CAAGGGGGAA GGGAAGGAGA GATGAATTAG CTTCCCCTGT
     4741 AAACCTTAGA ACCCAGCTGT TGCCAGGGCA ACGGGGCAAT ACCTGTCTCT TCAGAGGAGA
     4801 TGAAGTTGCC AGGGTAACTA CATCCTGTCT TTCTCAAGGA CCATCCCAGA ATGTGGCACC
     4861 CACTAGCCGT TACCATAGCA ACTGCCTCTT TGCCCCACTT AATCCCATCC CGTCTGTTAA
     4921 AAGGGCCCTA TAGTTGGAGG TGGGGGAGGT AGGAAGAGCG ATGATCACTT GTGGACTAAG
     4981 TTTGTTCGCA TCCCCTTCTC CAACCCCCTC AGTACATCAC CCTGGGGGAA CAGGGTCCAC
     5041 TTGCTCCTGG GCCCACACAG TCCTGCAGTA TTGTGTATAT AAGGCCAGGG CAAAGAGGAG
     5101 CAGGTTTTAA AGTGAAAGGC AGGCAGGTGT TGGGGAGGCA GTTACCGGGG CAACGGGAAC
     5161 AGGGCGTTTC GGAGGTGGTT GCCATGGGGA CCTGGATGCT GACGAAGGCT CGCGAGGCTG
     5221 TGAGCAGCCA CAGTGCCCTG CTCAGAAGCC CCAAGCTCGT CAGTCAAGCC GGTTCTCCGT
     5281 TTGCACTCAG GAGCACGGGC AGGCGAGTGG CCCCTAGTTC TGGGGGCAGC TCTAGAGCGG
     5341 TACCGGATCC CCGGGTACCG GTGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
     5401 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGAG
     5461 GGGCGAGGGC GAGGGCGATG CCACCAACGG CAAGCTGACC CTGAAGTTCA TCAGTACTAC
     5521 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGAGCCACG GCGTGCAGGT
     5581 GTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
     5641 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCACCTACA AGACCCGCGC
     5701 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCGTCGACTT
     5761 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTTCAACA GCCACAACAT
     5821 CTATATCATG GCCGTCAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGCCACAA
     5881 CGTGGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA
     5941 CGGCCCCGTG CTGCTGCCCG ACAGCCACTA CCTGAGCACC CAGTCCAAGC TTAGCAAAGA
     6001 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC CGCACCGCCG CCGGGATCAC
     6061 TCTCGGCATG GACGAGCTGT ACAAGTAAGG CGCGCCCCCC CCTAACGTTA CTGGCCGAAG
     6121 CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC
     6181 TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG
     6241 TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC
     6301 TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC
     6361 CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA
     6421 GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT
     6481 CTCCTCAAGC GTATTCAACA AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG
     6541 ATCTGATCTG GGGCCTCGGT GCACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG
     6601 TCTAGGCCCC CCGAACCACG GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG
     6661 CCACAACCAT GAGCCAGTTC GACATCCTGT GCAAGACCCC CCCCAAGGTG CTGGTGCGGC
     6721 AGTTCGTGGA GAGATTCGAG AGGCCCAGCG GCGAGAAGAT CGCCAGCTGT GCCGCCGAGC
     6781 TGACCTACCT GTGCTGGATG ATCACCCACA ACGGCACCGC CATCAAGAGG GCCACCTTCA
     6841 TGAGCTACAA CACCATCATC AGCAACAGCC TGAGCTTCGA CATCGTGAAC AAGAGCCTGC
     6901 AGTTCAAGTA CAAGACCCAG AAGGCCACCA TCCTGGAGGC CAGCCTGAAG AAGCTGATCC
     6961 CCGCCTGGGA GTTCACCATC ATCCCTTACA ACGGCCAGAA GCACCAGAGC GACATCACCG
     7021 ACATCGTGTC CAGCCTGCAG CTGCAGTTCG AGAGCAGCGA GGAGGCCGAC AAGGGCAACA
     7081 GCCACAGCAA GAAGATGCTG AAGGCCCTGC TGTCCGAGGG CGAGAGCATC TGGGAGATCA
     7141 CCGAGAAGAT CCTGAACAGC TTCGAGTACA CCAGCAGGTT CACCAAGACC AAGACCCTGT
     7201 ACCAGTTCCT GTTCCTGGCC ACATTCATCA ACTGCGGCAG GTTCAGCGAC ATCAAGAACG
     7261 TGGACCCCAA GAGCTTCAAG CTGGTGCAGA ACAAGTACCT GGGCGTGATC ATTCAGTGCC
     7321 TGGTGACCGA GACCAAGACA AGCGTGTCCA GGCACATCTA CTTTTTCAGC GCCAGAGGCA
     7381 GGATCGACCC CCTGGTGTAC CTGGACGAGT TCCTGAGGAA CAGCGAGCCC GTGCTGAAGA
     7441 GAGTGAACAG GACCGGCAAC AGCAGCAGCA ACAAGCAGGA GTACCAGCTG CTGAAGGACA
     7501 ACCTGGTGCG CAGCTACAAC AAGGCCCTGA AGAAGAACGC CCCCTACCCC ATCTTCGCTA
     7561 TCAAGAACGG CCCTAAGAGC CACATCGGCA GGCACCTGAT GACCAGCTTT CTGAGCATGA
     7621 AGGGCCTGAC CGAGCTGACA AACGTGGTGG GCAACTGGAG CGACAAGAGG GCCTCCGCCG
     7681 TGGCCAGGAC CACCTACACC CACCAGATCA CCGCCATCCC CGACCACTAC TTCGCCCTGG
     7741 TGTCCAGGTA CTACGCCTAC GACCCCATCA GCAAGGAGAT GATCGCCCTG AAGGACGAGA
     7801 CCAACCCCAT CGAGGAGTGG CAGCACATCG AGCAGCTGAA GGGCAGCGCC GAGGGCAGCA
     7861 TCAGATACCC CGCCTGGAAC GGCATCATCA GCCAGGAGGT GCTGGACTAC CTGAGCAGCT
     7921 ACATCAACAG GCGGATCTGA GAATT
//
