LOCUS       pAAV-hSyn-fDIO_mEGFP_GPHN_P1 7154 bp   circular         28-NOV-2022
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_C4c 7175 bp   circular     03-DEC-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      29-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      23-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      22-OCT-2020
EMBL_ID     pAAV-hSyn-mScarlet_GPHN_P2_XcmI_XhoI 6852 bp   circular 04-MAY-2020
EMBL_ID     pAAV-hSyn-mScarlet_GPHN_P2_XcmI_XhoI 6852 bp   circular 07-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      22-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_P1 7133 bp   circular      28-OCT-2020
EMBL_ID     pAAV-hSyn-fDIO_mScarlet_GPHN_C4a 7187 bp   circular     03-DEC-2020
EMBL_ID     pAAV-hSyn-fDIO_mEGFP_GPHN_P1 7154 bp   circular         03-DEC-2020
UNIMARK     pAAV-hSyn-fDIO_mEGFP_GPHN_P1 features
            pAAV-hSyn-fDIO_mEGFP_GPHN_P1
FEATURES             Location/Qualifiers
     misc_feature    1..7196
                     /comment="Molecule is created with Unipro UGENE v34.0"
                     /ugene_name="source"
     primer_bind     38..59
                     /label="F1ori-F"
                     /note="F1 origin, forward primer"
     primer_bind     complement(213..232)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     332..354
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(392..410)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        478..582
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             583..1443
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     andrelated antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     primer_bind     complement(801..820)
                     /label="Amp-R"
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1614..2202
                     /direction="RIGHT"
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2103..2122
                     /label="pBR322ori-F"
                     /note="pBR322 origin, forward primer"
     repeat_region   2264..2393
                     /label="AAV2 ITR (alternate)"
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        2438..2885
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     regulatory      2899..2908
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong
                     initiation of translation (Kozak, 1987)"
     misc_feature    2923..2956
                     /ugene_name="FRT_1"
                     /ugene_group="repeat_region"
     primer_bind     complement(3185..3203)
                     /label="mCherry-R"
                     /note="mCherry, reverse primer"
     primer_bind     3453..3472
                     /label="mCherry-F"
                     /note="mCherry, forward primer"
     misc_feature    complement(5284..6000)
                     /ugene_name="mEGFP"
     misc_feature    6164..6640
                     /ugene_name="polyA_signal"
     misc_feature    6680..6809
                     /ugene_name="repeat_region"
ORIGIN
        1 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA
       61 ACAACACTCA ACTCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG
      121 GTCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA
      181 TTAACGTTTA CAATTTTATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA
      241 AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG
      301 GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA
      361 CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT
      421 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC
      481 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA
      541 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC
      601 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA
      661 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA
      721 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG
      781 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA
      841 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC
      901 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC
      961 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA
     1021 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG
     1081 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA
     1141 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA
     1201 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC
     1261 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA
     1321 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA
     1381 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG
     1441 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA
     1501 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
     1561 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT
     1621 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG
     1681 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA
     1741 GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC
     1801 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
     1861 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG
     1921 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC
     1981 GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG
     2041 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA
     2101 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
     2161 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC
     2221 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTCCTGCAG GCAGCTGCGC
     2281 GCTCGCTCGC TCACTGAGGC CGCCCGGGCG TCGGGCGACC TTTGGTCGCC CGGCCTCAGT
     2341 GAGCGAGCGA GCGCGCAGAG AGGGAGTGGC CAACTCCATC ACTAGGGGTT CCTGCGGCCG
     2401 CACGCGTGTG TCTAGACTGC AGAGGGCCCT GCGTATGAGT GCAAGTGGGT TTTAGGACCA
     2461 GGATGAGGCG GGGTGGGGGT GCCTACCTGA CGACCGACCC CGACCCACTG GACAAGCACC
     2521 CAACCCCCAT TCCCCAAATT GCGCATCCCC TATCAGAGAG GGGGAGGGGA AACAGGATGC
     2581 GGCGAGGCGC GTGCGCACTG CCAGCTTCAG CACCGCGGAC AGTGCCTTCG CCCCCGCCTG
     2641 GCGGCGCGCG CCACCGCCGC CTCAGCACTG AAGGCGCGCT GACGTCACTC GCCGGTCCCC
     2701 CGCAAACTCC CCTTCCCGGC CACCTTGGTC GCGTCCGCGC CGCCGCCGGC CCAGCCGGAC
     2761 CGCACCACGC GAGGCGCGAG ATAGGGGGGC ACGGGCGCGA CCATCTGCGC TGCGGCGCCG
     2821 GCGACTCAGC GCTGCCTCAG TCTGCGGTGG GCAGCGGAGG AGTCGTGTCG TGCCTGAGAG
     2881 CGCAGTCGAG AAGGTACCGG ATCCTCTAGA GTCGACTCCG GAGAAGTTCC TATTCTCTAG
     2941 AAAGTATAGG AACTTCGCAG AATGGTAGCT GGATTGTAGC TGCTATTAGC AATATGAAAC
     3001 CTCTTAGAAG TTCCTATTCT TCAAAAGGTA TAGGAACTTC GGCGCGCCTC ATAGCCGTCC
     3061 GATGACCATG ACGTCCACCA CCTCGCCTTT GTGAAGTTCC ACATACTGCT CTGTCTTTGG
     3121 AGGTAGCATC AGCAATCCAT TGGCACTGCG CATGCTCATC AGACGGCTGC TCATCTGATT
     3181 ACCTGTACTC TGGGCCCAGG GCAGTGGTTC TTGGTGATGC CAAGTCAGTA TACACCGGTG
     3241 GTATTCTGGG CGAGGGTCCA GCTTTACGTC ACATGATAAC CTAGCTTTGA TGATGGTTGG
     3301 CCGAGGATCC AAGATACCCT GCATTTTTCT CAGTGCGGGT ACAACAAAGA GGTTGCAGGT
     3361 GACCACAGCT GATACAGGAT TCCCTGGTAG TGCAAAAATA ATTTTTCTTA CACCATCAAT
     3421 ATCCAAAGTT GCAAATGTTG TTGGCAAGCC TGGCTTCATA AAAACCCTGC CAAAATGGAT
     3481 CTGAGCATGA AGATCGATGT CCAGCACCTG CTTGAGATAG TCCTTTTCCC CCATGGATAC
     3541 ACCTCCTGAC GTGATGATGA CATCAGCACG GCTGATACCC TCATTCAAGG CATTGAGTAA
     3601 GTCATCTGGG TTGTCTCCTA CAATTCCCAA GTTGATTGTT GGGTAACCAT GTTCCTGAAT
     3661 TGTTGCTAGA AGTGTTGATC GATTGCTGTC CCGAATCTTT CCTGGTAAGA GGTCATCTTC
     3721 AGGATTTAGT AGCTCATTCC CTGTTGACAT AACGGCAACC ACTGGAAACT TATTAACTTC
     3781 AACTTCTGTG ACACCTACAG TTGCAAGGAG ACCAATTTCT GAAGGGCCCA TATGGGTTCC
     3841 TTTTGCCAAA ACACACTCCC CTCTTTTAAT GTCGTGGCCG ATGGGTCTGA TGTCTTGGCC
     3901 TGGCCGAGCT TGCACCAGAA TTCGCACTTC AAGTTCTTCA GTACCATCAT CTGATTCCCT
     3961 GATGAGTTCA GTATCTTCAA CTTGCACTAC TGCATCAGCA CCGCAGGGGA TTGGAGCACC
     4021 TGTTGTAACC CGCATCACTT GTCCGGGCAT TACTGTCTGA GTTGGCTGTT CACCAGCTTG
     4081 GGATTCCCCA ATGATGAAAC GATCTCCTGG ACCATCAGCA GCTCGAACAG CATAGCCATC
     4141 TTTTACTGAT GCTGGGAATG GGGGTAGGTT GTCTTTTGCA TACACATCTT GAGCAAGGAC
     4201 TCGCCCCATT CCATCTCGGT AATTGATGAT TTCTGTACCA AGCACCGGAG TCATCTCCAG
     4261 AACTGTAATG AAGGCTTTGT CCATAGACGT CAGAGGAAAA GGAGACATGC GATGTCTTCT
     4321 AGCCACCTTG GTGATATCTA CAGCACTGTG ACTGGCTCTT AGAATGTTCT CCTTGCTGCT
     4381 GCACCTGGAC TGGACTTTTG GTGTTGGACA AGAAGCTGTA GAGAGGCGAG ATGTAGCCTG
     4441 GGCACGGGGC GACTCTGAAG GAGTAGTGCT AAGGGAGGCT GTGTCTCGTG GGAGCACCTG
     4501 AACGCCACGA GAAATGATGG AGTCTGGAAT CTTTGCAGCA AGAGCTGCTG CAGTAATATG
     4561 TGATGAGGAA CTATCTTCTG TCGAAGCTAC ACCACTGTCT TTCTTTTCTT CTTCCTCCTC
     4621 TTCACACTGA ACTCCTTTGT CTTCTGTCTG CTTATGTGGG CTAGTTGTAG GAGGTGGAGA
     4681 AAGAGGAGGT GGTGGGGAAG GTAAATCTTC AAGTTCATCA TGCACCTCCT TTACTTTTAC
     4741 AATGGCATCA CGTAAAAGGT CAATGGCATG AGGTAGAGCT GGCAGTATGA ACTGAAAGCA
     4801 TTCCTGAGAT CCTTTCTTGC TACCAGGCAG GTTAATTATC AGAGTTTTCC CTCTTATTCC
     4861 ACACACTGGT CTAGAGAGCA TGCCCAGAGG TGTAACATTA AGTGATCCCA TCAGCATTGC
     4921 CAGGGCCATC CCTGGCGCTT CCCGTTCTAT TACTTCTTTT GTGGCCTCTG GAGTGACATC
     4981 TCGTGGTGCA AACCCTGTTC CGCCAGTTGT TAATATCAAA TTAAGTTCCT TCTCATCACA
     5041 CCAGTCTATG AGTGTTTCCT TGATTTCTTC TATTTCATCT GGTACTATCT TGTATGCTGA
     5101 TATAGTCCCA CCCAACAAAG AAGGATCCTG GACGAGATCT TTTAGATTTA TCCCACTGCG
     5161 GTCTTCTGCA AGATTCCTGA AGCAGCTATC ACTCACTGTG AGGACTCCGA CACGGATTTG
     5221 ATGGTCGTGG TTTGTGAGGA TCATTCCCTC GGTCGCCATG CTCGAGATCT GAGTCCGGCC
     5281 GGACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCTTCAG
     5341 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA
     5401 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC
     5461 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC
     5521 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT
     5581 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC
     5641 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA
     5701 GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG
     5761 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG
     5821 GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC
     5881 GTAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC
     5941 GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT
     6001 GGTGGCGCTA GCGAAGTTCC TATACTTTCT AGAGAATAGG AACTTCTTGC CTTAACCCAG
     6061 AAATTATCAC TGTTATTCTT TAGAATGGTG CAAAGAGAAG TTCCTATACC TTTTGAAGAA
     6121 TAGGAACTTC GAATTCGATA TCAAGCTTAT CGATGAGATC TACGGGTGGC ATCCCTGTGA
     6181 CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT
     6241 CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG
     6301 GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG
     6361 GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC
     6421 CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG
     6481 CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC
     6541 CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT
     6601 GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTGT AGGTAACCAC
     6661 GTGCGGACCG AGCGGCCGCA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC
     6721 GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG
     6781 GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC TGCCTGCAGG GGCGCCTGAT GCGGTATTTT
     6841 CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGTC AAAGCAACCA TAGTACGCGC
     6901 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC
     6961 TTGCCAGCGC CTTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG
     7021 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT
     7081 TACGGCACCT CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC
     7141 CCTGATAGAC GGTT
//
