LOCUS       Exported                1913 bp ds-DNA     linear   SYN 01-DEC-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 2, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             146..1669
                     /codon_start=1
                     /label=BtCreb5
                     /translation="MIYEESKMNLEQERPFVCSAPGCSQRFPTEDHLMIHRHKHEMTLK
                     FPSIKTDNMLSDQTPTPTRFLKNCEEVGLFSELDCSLEHEFRKAQEEESSKRNISMHNP
                     VGGAMAGPGAHQLGSARMPNHDTSVVIQQAMPSPQSSSVITQAPSTNRQIGPVPGSLSS
                     LLHLHNRQRQPMPASMPGTLPNPTMPGSSAVLMPMERQMSVNSNLLGMQGPNLSNPCAS
                     PQVQPMHSEAKMRLKAALTHHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQH
                     QTLPAHHPYPHQHQHPAHHPHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHSGTQ
                     AQVSPATQQMQPTQTIQPPQPTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVW
                     VMSLEKKAEELTQTNMQLQNEVSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPES
                     SPPASPTPACSQQQVIQHNTITTSSAVSEVVGSSTLSQLTTHRTDLNPIL"
     misc_feature    146..148
                     /label=ATG Start Codon
     primer_bind     513..530
                     /label=BtCreb5_qRTF3
     primer_bind     complement(654..671)
                     /label=BtCreb5_qRTR3
     misc_feature    1670..1675
                     /label=GlyGly Linker
     misc_feature    1676..1762
                     /label=3xHA Sequence
     misc_feature    1763..1765
                     /label=TAA Stop Codon
     gap             1899..1913
                     /estimated_length=15
ORIGIN
        1 nnnnnnncnn ntgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
       61 gcaatgcttt tttataatgc caactttgta caaaaaagca ggctccgcgg ccgccttgtt
      121 taactttaag aaggagccct tcaccatgat ttatgaggaa tccaagatga atttggagca
      181 ggagaggccg tttgtctgca gtgccccagg ctgctcccag cgcttcccaa cagaggatca
      241 tctgatgatt cataggcaca agcatgaaat gactttgaag tttccctcaa taaaaacaga
      301 caatatgtta tcagatcaaa ctccaacccc aacgagattc ctgaagaact gcgaggaggt
      361 ggggctcttc agtgagttgg actgctccct agagcatgag ttcaggaagg ctcaggagga
      421 agagagcagc aagcggaata tctcgatgca taaccccgtt ggtggggcca tggcggggcc
      481 tggagctcac cagctcggca gcgcccggat gcccaaccat gacaccagcg ttgtgattca
      541 gcaagccatg ccgtcacccc agtccagctc tgtcatcacg caggcccctt ccaccaaccg
      601 ccagatcggg cctgtcccag gctctctatc ttctctgctc catcttcaca acagacagag
      661 acagcccatg ccagcctcca tgcctgggac cctgcccaac cctacaatgc cgggctcttc
      721 tgccgtcttg atgccaatgg agagacaaat gtcagtgaac tccaacctcc tgggaatgca
      781 aggtccaaat ctcagcaacc cctgtgcttc tccccaagtc cagccaatgc attcagaagc
      841 taagatgagg ctgaaggctg cattgactca ccaccctgct gccatgtcga atgggaatat
      901 gaacaccatg ggccacatga tggaaatgat gggctcccgg caggaccaga cgccacacca
      961 tcacatgcac tcacacccgc atcagcacca gacactgccg gcccaccacc cctacccgca
     1021 ccagcaccag cacccagcac accaccctca tcctcaaccc catcaccagc agaaccaccc
     1081 acatcatcac tcccactccc accttcatgc acacccagca catcaccaga cctcgccaca
     1141 cccacccctg cactccggca cccaagcaca ggtttcacca gcaacacaac agatgcagcc
     1201 cacccagaca atacagccgc cccagcccac aggggggcgc cggcgaaggg tggtggatga
     1261 ggatcctgac gagaggcggc ggaaatttct ggaacggaac cgggcggccg ccacccgctg
     1321 caggcagaag aggaaggtct gggtgatgtc actggaaaag aaagcagaag agctcaccca
     1381 gacaaacatg cagcttcaga acgaagtgtc catgttgaag aacgaggtgg cacagctgaa
     1441 gcagctgttg ttaacacata aagactgccc cataacagcc atgcagaaag aatcacaagg
     1501 atatctcagt ccagagagta gccctcctgc aagtcccacc cccgcatgct cacagcagca
     1561 ggtcatccag cataacacca tcaccacttc ctcggcagtc agcgaggtgg taggaagctc
     1621 taccctcagc cagctcacca ctcacagaac agacctgaac cccatcctcg gaggttaccc
     1681 ttacgacgtc ccagactacg ctggctccta cccttacgac gtcccagact acgcttaccc
     1741 ttacgacgtc ccagactacg cttaaaaggg tgggcgcgcc gacccagctt tcttgtacaa
     1801 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1861 tcaaaataaa atcattattt gccatccagc tganncccnn nnnnnnnnnn nnn
//
