LOCUS       Exported                1620 bp ds-DNA     linear   SYN 01-DEC-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 2, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Storage Type: Basic
            Original Author: 
            Demo User
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..3
                     /label=ATG
     misc_feature    175..177
                     /label=Threonine to Alanine Mutation
     misc_feature    181..183
                     /label=Threonine to Alanine Mutation
     misc_feature    1129..1146
                     /label=qRT_PrimerF
     misc_feature    1525..1530
                     /label=GlyGly Linker
     misc_feature    1531..1617
                     /label=3xHA Sequence
     misc_feature    1618..1620
                     /label=TAA
ORIGIN
        1 atgatttatg aggaatccaa gatgaatttg gagcaggaga ggccgtttgt ctgcagtgcc
       61 ccaggctgct cccagcgctt cccaacagag gatcatctga tgattcatag gcacaagcat
      121 gaaatgactt tgaagtttcc ctcaataaaa acagacaata tgttatcaga tcaagctcca
      181 gccccaacga gattcctgaa gaactgcgag gaggtggggc tcttcagtga gttggactgc
      241 tccctagagc atgagttcag gaaggctcag gaggaagaga gcagcaagcg gaatatctcg
      301 atgcataacc ccgttggtgg ggccatggcg gggcctggag ctcaccagct cggcagcgcc
      361 cggatgccca accatgacac cagcgttgtg attcagcaag ccatgccgtc accccagtcc
      421 agctctgtca tcacgcaggc cccttccacc aaccgccaga tcgggcctgt cccaggctct
      481 ctatcttctc tgctccatct tcacaacaga cagagacagc ccatgccagc ctccatgcct
      541 gggaccctgc ccaaccctac aatgccgggc tcttctgccg tcttgatgcc aatggagaga
      601 caaatgtcag tgaactccaa cctcctggga atgcaaggtc caaatctcag caacccctgt
      661 gcttctcccc aagtccagcc aatgcattca gaagctaaga tgaggctgaa ggctgcattg
      721 actcaccacc ctgctgccat gtcgaatggg aatatgaaca ccatgggcca catgatggaa
      781 atgatgggct cccggcagga ccagacgcca caccatcaca tgcactcaca cccgcatcag
      841 caccagacac tgccggccca ccacccctac ccgcaccagc accagcaccc agcacaccac
      901 cctcatcctc aaccccatca ccagcagaac cacccacatc atcactccca ctcccacctt
      961 catgcacacc cagcacatca ccagacctcg ccacacccac ccctgcactc cggcacccaa
     1021 gcacaggttt caccagcaac acaacagatg cagcccaccc agacaataca gccgccccag
     1081 cccacagggg ggcgccggcg aagggtggtg gatgaggatc ctgacgagag gcggcggaaa
     1141 tttctggaac ggaaccgggc ggccgccacc cgctgcaggc agaagaggaa ggtctgggtg
     1201 atgtcactgg aaaagaaagc agaagagctc acccagacaa acatgcagct tcagaacgaa
     1261 gtgtccatgt tgaagaacga ggtggcacag ctgaagcagc tgttgttaac acataaagac
     1321 tgccccataa cagccatgca gaaagaatca caaggatatc tcagtccaga gagtagccct
     1381 cctgcaagtc ccacccccgc atgctcacag cagcaggtca tccagcataa caccatcacc
     1441 acttcctcgg cagtcagcga ggtggtagga agctctaccc tcagccagct caccactcac
     1501 agaacagacc tgaaccccat cctcggaggt tacccttacg acgtcccaga ctacgctggc
     1561 tcctaccctt acgacgtccc agactacgct tacccttacg acgtcccaga ctacgcttaa
//
