LOCUS       Exported                3973 bp ds-DNA     circular SYN 29-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    natMX6-ins5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Unal
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2022 from SnapGene 3.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    42..61
                     /note="F1 pringle primer"
     misc_feature    complement(62..111)
                     /label=DEG1-insulator
                     /note="DEG1-insulator"
     misc_feature    130..508
                     /note="TEF promoter"
     CDS             509..1081
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /note="NrsR"
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      1087..1284
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    1315..1536
                     /note="CYC1 insulator"
     misc_feature    complement(1537..1556)
                     /note="R1 primer pringle primer"
     promoter        complement(1611..1629)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1887..2475)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2646..3506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3507..3611)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3957..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 agaaaagaaa tatagtcttc aaggttatat tatacaggtt tatatattat tggcgcgcca
      121 gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga
      181 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
      241 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
      301 cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc
      361 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
      421 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
      481 ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca cggcttaccg
      541 gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg ggtccttcac
      601 caccgacacc gtcttccgcg tcaccgccac cggggacggc ttcaccctgc gggaggtgcc
      661 ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg aatcggacga
      721 cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg acggcgacct
      781 ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg tcgaggacat
      841 cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac
      901 ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca acgtcaacgc
      961 accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg acaccgccct
     1021 gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc cctgccccta
     1081 atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt
     1141 tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc
     1201 gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt
     1261 atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca tccaacaggc
     1321 cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctccc
     1381 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     1441 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     1501 tttctgtaca aacgcgtgta cgcatgtaac attatagttt aaacgagctc gaattcatcg
     1561 atgatatcag atccactagt ggcctatgcg gccgcggatc tgccggtctc cctatagtga
     1621 gtcgtattaa tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg
     1681 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1741 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1801 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1861 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1921 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1981 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2041 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     2101 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2161 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2221 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2281 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2341 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2401 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2461 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2521 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2581 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2641 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2701 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2761 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2821 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2881 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2941 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3001 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3061 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3121 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3181 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3241 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3301 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3361 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3421 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3481 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3541 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3601 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     3721 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     3781 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     3841 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatggacata
     3901 ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
     3961 aggtgacact ata
//
