LOCUS       Exported                4073 bp ds-DNA     circular SYN 15-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    natMX-ins7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Unal
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2022 from SnapGene 3.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    42..61
                     /note="F1 pringle primer"
     misc_feature    complement(62..283)
                     /note="CYC1 insulator"
     misc_feature    284..663
                     /note="pPGK1 from Candida glabrata"
     CDS             664..1236
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /note="NrsR"
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     misc_feature    1237..1636
                     /note="tPGK1 from Candida glabrata"
     misc_feature    complement(1637..1656)
                     /note="R1 primer pringle primer"
     promoter        complement(1711..1729)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1987..2575)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2746..3606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3607..3711)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        4057..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 atataatgtt acatgcgtac acgcgtttgt acagaaaaaa aagaaaaatt tgaaatataa
      121 ataacgttct taatactaac ataactataa aaaaataaat agggacctag acttcaggtt
      181 gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg
      241 acataactaa ttacatgata tcgacaaagg aaaaggggcc tgtgaagctc taggagaagc
      301 cctaggagaa gcaaagggga agcccaaaga gggacaagcg caggccacta cagtaacaaa
      361 gacaggaagc ccaaaagatc gcaccttcca gtccatcgcc ttcccccatt gtcatatggc
      421 agtttatagc agtgtggcag tatggcagtg tggcaatgtg aagcagtgtg caatacttcc
      481 aaagtggata atgtgatgct tgcaaaaagt tgggaccacc aatgctgtag agacaatggg
      541 ataccctagc gaatgaagaa aaaaaggaaa acgaaaaaaa tttgtatata aagcagaggc
      601 aatttttgta gcggcatgat tagttgtcaa ttggcaagac ggcatacata catctattcg
      661 ataatgggta ccactcttga cgacacggct taccggtacc gcaccagtgt cccgggagac
      721 gccgaagcca tcgaggcact ggatgggtcc ttcaccaccg acactgtctt ccgcgtcact
      781 gccaccgggg acggcttcac cctgcgggag gtgccggtgg acccgccact gaccaaggtg
      841 ttccccgatg acgagtcgga tgacgaatct gacgacggtg aggacggcga cccagactcc
      901 cggacgttcg tcgcgtacgg ggacgacggc gacctagcgg gattcgtggt cgtctcgtac
      961 tccggctgga accgacggct gaccgtcgag gacatcgaag tagccccaga acaccgaggg
     1021 cacggagtcg ggcgagcgtt gatgggtctc gcgacggagt tcgctcgcga gcggggcgca
     1081 ggtcacctat ggctagaggt caccaacgtc aacgcaccag cgatccacgc gtaccgacgg
     1141 atggggttca ccctctgcgg tctggacacc gcactgtacg acggcacagc ctcggacggc
     1201 gagcaggcgc tctacatgag catgccctgc ccctaaacta gcttgagaac tccccctagt
     1261 tcttagaaag gtgtaatttg agcgcatgaa taatatttct ccactcccga tacttttaat
     1321 agggtagtaa aaaatttaac cagttcgagt gcttcctaac aagatgaaca taaacttgat
     1381 agagtctacc ttttgatagg gtataaattt tatcttcctg tttagagatg ttttctcaat
     1441 ggttaatgga aaatatatgc tatatcttgc cacgcagtac atcctttcga agctttttta
     1501 ttctatttca acacatcgat aaatgacgta cataaaaatt ataaataatt taatgaaaaa
     1561 attattatta tgacttatac attaattaat tatcagttta tttttttttt attttttttc
     1621 tttctcagtt taattagttt aaacgagctc gaattcatcg atgatatcag atccactagt
     1681 ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa tttcgataag
     1741 ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1801 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1921 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1981 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2041 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2101 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2161 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2221 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2281 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2341 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2401 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2461 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2521 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2581 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2641 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2701 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2761 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2821 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2881 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2941 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3001 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3061 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3121 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3181 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3241 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3301 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3361 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3421 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3481 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3541 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3601 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3661 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3721 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3781 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     3841 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     3901 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     3961 atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta
     4021 caattaatac ataaccttat gtatcataca catacgattt aggtgacact ata
//
