LOCUS       pBL090j_-_[P15A]_PBAD_s 3510 bp ds-DNA     circular     17-SEP-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter             433..472
                          /label="RNA I Promoter (0.96)"
                          /ApEinfo_revcolor="#c4df9b"
                          /ApEinfo_fwdcolor="#c4df9b"
     -35_signal           438..443
                          /label="-35 -35 signal"
                          /ApEinfo_revcolor="#d6b295"
                          /ApEinfo_fwdcolor="#d6b295"
     -10_signal           462..467
                          /label="-10 -10 signal"
                          /ApEinfo_revcolor="#84b0dc"
                          /ApEinfo_fwdcolor="#84b0dc"
     misc_RNA             474..577
                          /label="RNA I"
                          /ApEinfo_revcolor="#84b0dc"
                          /ApEinfo_fwdcolor="#84b0dc"
     promoter             complement(584..623)
                          /label="RNA II Promoter (0.92)"
                          /ApEinfo_revcolor="#c4df9b"
                          /ApEinfo_fwdcolor="#c4df9b"
     -10_signal           complement(589..594)
                          /label="-10 -10 signal"
                          /ApEinfo_revcolor="#75c6a9"
                          /ApEinfo_fwdcolor="#75c6a9"
     -35_signal           complement(612..617)
                          /label="-35 -35 signal"
                          /ApEinfo_revcolor="#85dae9"
                          /ApEinfo_fwdcolor="#85dae9"
     CDS                  complement(729..1607)
                          /label="araC"
                          /ApEinfo_revcolor="#6ecff6"
                          /ApEinfo_fwdcolor="#6ecff6"
     protein binding site 1636..1652
                          /label="araO2"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     prim_transcript      1771..1771
                          /label="pC TSS"
                          /ApEinfo_revcolor="#b1ff67"
                          /ApEinfo_fwdcolor="#b1ff67"
     protein_bind         1773..1789
                          /label="araO1"
                          /ApEinfo_revcolor="#b4abac"
                          /ApEinfo_fwdcolor="#b4abac"
     protein binding site 1794..1810
                          /label="araO1"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     protein binding site 1815..1836
                          /label="CAP"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     promoter             1815..1919
                          /label="pBAD (truncated)"
                          /ApEinfo_revcolor="#c4df9b"
                          /ApEinfo_fwdcolor="#c4df9b"
     protein binding site 1847..1863
                          /label="araI1"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     protein binding site 1868..1884
                          /label="araI2"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     -35                  1881..1886
                          /label=-35
                          /ApEinfo_revcolor="#85dae9"
                          /ApEinfo_fwdcolor="#85dae9"
     -10                  1905..1910
                          /label=-10
                          /ApEinfo_revcolor="#f58a5e"
                          /ApEinfo_fwdcolor="#f58a5e"
     primary transcript   1919..1919
                          /label="pBAD +1"
                          /ApEinfo_revcolor="#82ca9d"
                          /ApEinfo_fwdcolor="#82ca9d"
     RBS                  1946..1959
                          /label="sd8"
                          /ApEinfo_revcolor="#b7e6d7"
                          /ApEinfo_fwdcolor="#b7e6d7"
     gene                 1960..2424
                          /label="cadC 1-155"
                          /ApEinfo_revcolor="#f8d3a9"
                          /ApEinfo_fwdcolor="#f8d3a9"
     CDS                  1960..2517
                          /label="Translation 1960-2517"
                          /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLRPRLIDLLVFFAQHSGEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMVPVIWYSEEEGEEIMLSSAAAISEAVPATDSPSHSLNIQNTATPPEQSPVKSKRGGSGGLKQKIAALKYKNAALKKKIAALKQG*"
     misc_feature         2047..2049
                          /label="E30R"
                          /ApEinfo_revcolor="#f58a5e"
                          /ApEinfo_fwdcolor="#f58a5e"
     misc_feature         2317..2331
                          /label="AAAIS mutation"
                          /ApEinfo_revcolor="#f58a5e"
                          /ApEinfo_fwdcolor="#f58a5e"
     misc_feature         2425..2439
                          /label="GGSGG Linker"
                          /ApEinfo_revcolor="#75c6a9"
                          /ApEinfo_fwdcolor="#75c6a9"
     misc_feature         2440..2517
                          /label="CCB3.5"
                          /ApEinfo_revcolor="#84b0dc"
                          /ApEinfo_fwdcolor="#84b0dc"
     terminator           complement(2530..2563)
                          /label="BBa_B1002 terminator"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     terminator           complement(2564..2605)
                          /label="tetA/orfL bidirectional terminator"
                          /ApEinfo_revcolor="#f6989d"
                          /ApEinfo_fwdcolor="#f6989d"
     CDS                  complement(2626..3441)
                          /label="KanR"
                          /ApEinfo_revcolor="#6ecff6"
                          /ApEinfo_fwdcolor="#6ecff6"
     promoter             complement(3442..3492)
                          /label="KanR promoter"
                          /ApEinfo_revcolor="#c4df9b"
                          /ApEinfo_fwdcolor="#c4df9b"
ORIGIN
        1 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
       61 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
      121 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
      181 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      241 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      301 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
      421 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
      481 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
      541 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
      601 gaccaaaacg atctcaagaa gatcatctta ttaaggagcc ccatacgata tacgagagga
      661 catccgggca aatgcaacga aaggctcagt cgaaagactg ggcctttcgt tgcagactta
      721 gggacccttt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg
      781 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg
      841 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc
      901 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg
      961 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata
     1021 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta
     1081 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca
     1141 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt
     1201 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta
     1261 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa
     1321 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct
     1381 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc
     1441 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg
     1501 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca
     1561 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc
     1621 ccaccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc
     1681 ttttactggc tcttctcgct aacccaaccg gtaaccccgc ttattaaaag cattctgtaa
     1741 caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga
     1801 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc
     1861 ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccataC
     1921 CCGTTTTTTT ACCTGCAGGT GCAGTaaagg aaaaaaaaaa tgcaacaacc tgtagttcgc
     1981 gttggcgaat ggcttgttac tccgtccata aaccaaatta gccgcaatgg gcgtcaactt
     2041 acccttCGTc cgagattaat cgatcttctg gttttctttg ctcaacacag tggcgaagta
     2101 cttagcaggg atgaacttat cgataatgtc tggaagagaa gtattgtcac caatcacgtt
     2161 gtgacgcaga gtatctcaga actacgtaag tcattaaaag ataatgatga agatagtcct
     2221 gtctatatcg ctactgtacc aaagcgcggc tataaattaa tggtgccggt tatctggtac
     2281 agcgaagaag agggagagga aataatgcta tcttcgGCAG CGGCTATCTC Tgaggcggtt
     2341 cctgccacag attctccctc ccacagtctt aacattcaaa acaccgcaac gccacctgaa
     2401 caatccccag ttaaaagcaa acgaggtggA TCTGGTGGTc tgaaacagaa gatcgccgca
     2461 ctgaaataca agaatgcggc cctgaaaaag aagatcgcgg cactgaaaca gggttaaATT
     2521 AACGGCACTG CGAAAAAACC CCGCCGAAGC GGGGTTTTTT GCGAAATATA ATGACCCTCT
     2581 TGATAACCCA AGAGGGCATT TTTTAatgcc catggcgtac ggatcttaga aaaactcatc
     2641 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     2701 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     2761 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     2821 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     2881 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     2941 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     3001 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     3061 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     3121 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     3181 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     3241 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     3301 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     3361 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt
     3421 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     3481 tattgttcat gaagagaaac ctacggatcg
//