LOCUS       pBbA5a_sufCDSUB         8879 bp    DNA     circular SYN 09-JAN-2023
DEFINITION  B. subtils SUF cloned into pBbA5a.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  E.coli expression plasmid
            .
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   TUDelft
  TITLE     Direct Submission
  JOURNAL   Exported Sep 23, 2021 from SnapGene 5.3.2 https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     promoter        complement(1132..1209)
                     /gene="lacI"
     promoter        1520..1550
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1610..2395
     CDS             2413..3726
     CDS             3726..4946
     CDS             4936..5379
     CDS             5400..6797
     terminator      6830..6901
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6917..6944
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(7106..7651)
     terminator      7765..7859
                     /note="transcription terminator from phage lambda"
     CDS             complement(7889..8749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(8750..8854)
                     /gene="bla"
ORIGIN      
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atcttttgga ggtatagata tggctgcttc
     1621 aacattaacg atcaaagatc ttcacgttga aatcgaaggg aaagagatct taaagggtgt
     1681 aaaccttgaa ataaaaggtg gagaattcca cgcagtaatg ggcccgaacg gaactggtaa
     1741 atccacttta tcagctgcta ttatggggca tcctaaatat gaagtaacaa aaggcagcat
     1801 cacgcttgac ggcaaagatg tactggagat ggaagtggac gagcgtgcgc aggcaggctt
     1861 attccttgca atgcagtacc caagtgaaat cagcggtgtg acaaatgccg acttccttcg
     1921 ctcagcaatc aacgcgcgca gagaagaagg cgatgaaatt tctctcatga aattcatccg
     1981 caaaatggac gaaaacatgg agttccttga aatggaccct gaaatggctc agcgctatct
     2041 taacgaaggc ttctcaggcg gggagaaaaa acgcaacgaa atccttcaat taatgatgat
     2101 tgaaccgaaa atcgccatcc ttgatgaaat tgactcaggc cttgatattg acgctttgaa
     2161 agttgtatca aaagggatca acaaaatgcg cagcgaaaac ttcggctgcc tgatgatcac
     2221 tcactatcag cgcctgttaa actacatcac tccggatgtt gttcacgtta tgatgcaagg
     2281 ccgcgttgta aaatccggcg gtgcagagct tgcacagcgt cttgaagcag aaggttatga
     2341 ctggatcaaa caagaacttg gcattgaaga cgaaactgtt ggccaagaag cgtaagcgtt
     2401 agggggatta atatgacact aggtacaaaa ctatccgtag atcaggagta tctcaaaagc
     2461 ttttccgaaa agcaccaaga acctgcctgg ctgaagaacc tgcgcttaca ggctcttgaa
     2521 caagctgagg atctgccgat gccgaaacct gacaaaacaa aaatcacaaa ctggaacttc
     2581 acgaatttcg cgaagcatac agtggataac gaaccgcttt cttcattaga agatttgact
     2641 gatgaagtaa aagcgctgat cgacattgaa aacgaagata aaacattata cgtacagcgt
     2701 gaccagacgc cggcacatct ttctctttct caagaattga aagacaaagg cgtcatcttc
     2761 actgatattc tgactgctgc acgcgagcac agcgacttgg tggagaaata ctttatgaag
     2821 gacggcgtga aggttgacga acataagtta actgcacttc acgcggctct tgttaacgga
     2881 ggagcattcc tttatgttcc gaaaaatgtt caggtggaga cgccggttca ggctgtttat
     2941 gtccacgaaa gcaatgatac agctctattc aaccacgtgc tgattgtggc tgaagaccac
     3001 agctctgtaa catatgtaga aaactacatc agcactgtaa acccgaaaga cgctgtcttc
     3061 aatattatca gtgaggtcat cacaggtgat aatgccagcg tgacatacgg tgcggtggat
     3121 aatctgtcaa gcggtgtgac aacttatgtg aaccgccgcg gtgctgcgcg cggacgcgac
     3181 agcaaaatcg agtgggctct cggcctgatg aacgatggcg acacgatttc cgaaaacaca
     3241 acaaaccttt acggtgacgg cacatacggc gatacgaaaa cggttgttgt cggaagagga
     3301 gagcaaacag aaaacttcac aacacaaatc atccatttcg gtaaagcttc tgaaggctat
     3361 atcttgaagc acggtgtgat gaaggattcc gcttcttcta tctttaacgg aatcggcaaa
     3421 atcgaacatg gcgcttctaa agcgaatgcg gagcaagaat cacgcgtgct gatgcttagc
     3481 gaaaaagcac gcggagacgc aaaccctatt cttttaattg acgaagacga tgtaactgca
     3541 ggacatgcgg catctgtcgg ccgtgtggac cctattcagc tttactacct gatgagccgc
     3601 ggaattccga aagaagaagc agaacgctta gtcatttacg gattccttgc accggtagta
     3661 aatgaacttc cgattgaagg cgttaagaaa caattagttt ctgttatcga aaggaaagtg
     3721 aagtaatgaa tatcacagat attcgtgaac agttcccgat ccttcatcag caagtgaacg
     3781 gacatgatct cgtttatctc gacagcgcgg cgacttccca gaagccgcgt gctgtcattg
     3841 aaacactgga taagtattat aaccaataca attccaatgt tcaccggggc gtccatacac
     3901 ttggaaccag agcgacagat ggttatgaag gagcgcgtga aaaagtccgc aagtttatta
     3961 acgcgaagtc aatggctgag attattttca caaaaggcac gaccacatca ctgaatatgg
     4021 tggcgttaag ctatgcgcgc gccaacctga aacctggtga tgaagtggtc atcacctaca
     4081 tggagcatca tgcgaatatc attccgtggc agcaggctgt aaaagcaact ggcgccacat
     4141 taaaatatat tccgttgcag gaagacggaa cgatttcttt agaagacgtc agagaaacgg
     4201 tcacaagcaa cacaaaaatt gttgcggtgt ctcatgtatc taatgttctc ggcacggtca
     4261 acccgattaa agaaatggcg aaaatcgctc atgacaatgg ggctgtcatt gtggtggacg
     4321 gtgcacaaag cacgcctcac atgaaaattg acgtgcagga tctcgattgc gacttctttg
     4381 cgctttcttc ccataaaatg tgcggaccta ccggtgttgg tgtgctgtac ggaaagaaag
     4441 ccctgctgga aaacatggag cctgccgaat tcggcggtga aatgatcgac tttgtcgggc
     4501 tttatgaatc aacttggaaa gagcttccgt ggaaattcga agcgggtacg ccgattattg
     4561 caggagcaat cggcctcggt gccgccattg attttctcga agaaatcggt cttgatgaga
     4621 tttcccgcca tgagcacaag cttgcagctt atgcgcttga acgcttccgc cagcttgacg
     4681 gcgtaacggt ttacgggccg gaagagcgtg ccgggcttgt cacatttaac cttgatgatg
     4741 ttcatccgca cgatgtggcg actgtgcttg atgcggaggg aatcgcagtc agagccggcc
     4801 accattgtgc gcagccgctg atgaaatggc tggatgtaac tgctactgca agagcaagct
     4861 tttatctgta taatacagag gaagagattg ataagcttgt ggaagctctt caaaagacaa
     4921 aggagtattt tacaaatgtc ttttaatgca aacttagata cattgtacag acaggtgatt
     4981 atggatcatt acaaaaaccc gcgaaataaa ggggttttaa atgacagcat tgtcgtggat
     5041 atgaacaatc cgacatgcgg cgaccgcatc agactgacaa tgaagcttga cggagacatc
     5101 gtggaagatg cgaagtttga aggggaaggc tgttccattt caatggcatc cgcttcgatg
     5161 atgactcagg cgattaaagg gaaagatatt gaaactgccc tttccatgtc taagattttc
     5221 tcggatatga tgcaagggaa agagtatgat gattctatag atctcgggga tattgaagcc
     5281 cttcaaggcg tttcaaaatt ccctgcccgt atcaaatgtg caaccctgtc atggaaagca
     5341 cttgaaaaag gagtcgcgaa agaagaaggc ggcaattaaa aggattggag tgaaaatgga
     5401 tggctaaaaa aatgcctgat attggtgaat acaagtatgg ttttcacgac aaggacgttt
     5461 ccattttccg ttcagagcgc ggattgacaa aagaaatcgt agaagaaatt tctcgcatga
     5521 aagaagagcc tcaatggatg cttgacttcc gcttgaaatc acttgagcat ttctacaata
     5581 tgccgatgcc tcaatggggc ggagatttaa actcactgaa ctttgacgaa attacgtact
     5641 acgtaaaacc gtctgagcgt tcagagcgtt cttgggatga agttcctgaa gaaattaaac
     5701 aaacattcga caagctcggt attcctgaag ctgaacaaaa ataccttgcg ggtgtttctg
     5761 ctcagtacga atctgaggtt gtataccaca acatgaaaga agatcttgaa gcacaaggca
     5821 tcgtctttaa agacactgac agcgcgctga aagagaatga agatattttc cgtgagcact
     5881 gggcaaaagt aatcccgccg actgataaca agtttgcggc gcttaactca gctgtttggt
     5941 ccggcggttc tttcatctac gtgcctaaag gcgtgaaggt tgaaacaccg ctacaagctt
     6001 acttccgtat caactctgag aacatgggtc agttcgaacg tacactgatc attgttgacg
     6061 aagaagcaag cgttcattat gttgaaggct gtactgcgcc tgtttacaca acaaactcac
     6121 ttcacagtgc ggtcgttgag atcatcgtga aaaaaggcgg ctactgccgt tatacaacga
     6181 tccaaaactg ggcgaacaac gtttacaacc ttgtaacaaa gcgtacagtc tgtgaagaaa
     6241 acgcaacaat ggaatggatc gacggcaaca tcggttctaa gctgacaatg aaatatccgg
     6301 cttgtatcct aaaaggcgaa ggcgcgcgcg gtatgacgct ttctatcgct cttgcgggta
     6361 aaggccagca tcaggatgca ggtgcgaaaa tgattcacct tgcaccaaac acatcatcta
     6421 caatcgtatc aaaatcgatt tcaaaacagg gcggaaaagt aacgtaccgc ggaatcgtcc
     6481 acttcggacg caaagcggaa ggcgcccgct ctaacatcga gtgtgatacg ctcattatgg
     6541 ataacaaatc aacttctgac acaattcctt acaatgaaat cttaaatgat aacatttcat
     6601 tggagcacga agcgaaagtg tctaaagtat ctgaagagca gctcttctac ttgatgagcc
     6661 gcggtatttc tgaagaagaa gcaactgaaa tgatcgtaat gggctttatc gagccattca
     6721 caaaagagct tccgatggaa tacgcagttg aaatgaaccg tttgattaag ttcgaaatgg
     6781 aaggttctat cggttaagga tcctaactcg agtaaggatc tccaggcatc aaataaaacg
     6841 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     6901 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata cctagggata
     6961 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
     7021 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
     7081 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     7141 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     7201 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     7261 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     7321 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
     7381 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
     7441 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
     7501 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
     7561 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
     7621 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
     7681 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
     7741 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     7801 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     7861 aagcgagctc gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7921 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7981 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8041 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8101 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8161 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8221 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8281 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8341 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8401 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8461 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8521 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8581 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8641 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8701 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8761 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8821 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacct
//
