LOCUS       pBbA5a_sufABCDSE        9225 bp    DNA     circular     09-JAN-2023
DEFINITION  .
ACCESSION   urn.local.gbokinsky.k-frpspqt
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     CDS             complement(49..1131)
                     /gene="<i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli. </i>This
                     inhibition can be relieved by adding lactose or
                     isopropyl-ß-D-thiogalactopyranoside (IPTG)."
                     /product="<i>lac</i> repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ*"
                     /locus_tag="lacI"
     promoter        complement(1132..1209)
                     /gene="<i>lacI</i>"
                     /note="/locus_tag=""lacI promoter"
     primer_bind     1462..1479
                     /PCR_conditions="primer sequence:TGATGCCGGCCACGATGC"
                     /note="<html><body></body></html>"
                     /locus_tag="#3"
     promoter        1520..1550
                     /note="<i>E. coli lac</i> promoter with an ""up""
                     mutation"
                     /locus_tag="lac UV5 promoter"
     protein_bind    1558..1574
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-ß-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
     misc_feature    1586..1600
                     /note="Geneious type: Editing History Insertion"
     CDS             1612..1980
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV*"
                     /locus_tag="SufA"
     CDS             1989..3476
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG*"
                     /locus_tag="SufB"
     misc_feature    2056
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2239
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2353
                     /note="Geneious type: Editing History Replacement"
     CDS             3486..4232
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVHPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ*"
                     /locus_tag="SufC"
     CDS             4207..5478
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLINSQFVSIAGEISPQQRDALALTLDSVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR*"
                     /locus_tag="SufD"
     CDS             5475..6695
                     /translation="MIFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG*"
                     /locus_tag="SufS"
     misc_feature    5490
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5896
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5929
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6587
                     /note="Geneious type: Editing History Replacement"
     CDS             6708..7124
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS*"
                     /locus_tag="SufE"
     misc_feature    6854
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6994
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7125..7152
                     /note="Geneious type: Editing History Insertion"
     terminator      7176..7247
                     /note="transcription terminator T1 from the <i>E. coli
                     rrnB</i> gene"
                     /locus_tag="rrnB T1 terminator"
     primer_bind     complement(7244..7266)
                     /PCR_conditions="primer sequence:AGCCAGTGTGACTCTAGTAGAGA"
                     /note="<html><body></body></html>"
                     /locus_tag="#4"
     terminator      7263..7290
                     /note="phage T7 early transcription terminator"
                     /locus_tag="T7Te terminator"
     rep_origin      complement(7452..7997)
     terminator      8111..8205
                     /gene="/note=""transcription terminator from phage lambda"
                     /locus_tag="lambda t0 terminator"
     CDS             complement(8235..9095)
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /product="ß-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVG
                     YIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLV
                     EYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGP
                     LLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQI
                     AEIGASLIKHW*"
                     /locus_tag="AmpR"
     promoter        complement(9096..9200)
                     /gene="<i>bla</i>"
                     /locus_tag="AmpR promoter"
ORIGIN      
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattctgaaa gcgatgaagt gaggtaaatc gatggacatg
     1621 cattcaggaa cctttaaccc acaagatttc gcctggcaag gcttaacgct gacacccgca
     1681 gcggcgatac acatccgtga gctggtggca aagcagccgg gtatggtcgg cgtgcgctta
     1741 ggcgtgaagc aaacgggctg cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg
     1801 gacaaagacg atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc gctgcaagcg
     1861 atgccgttta ttgatggcac ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc
     1921 aaatttcaca accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt tggggtatag
     1981 gcggtactat gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg accggcggcc
     2041 cgctgaatta taaaggagga ttcttcaccc agttagccac cgatgagctg gcaaagggga
     2101 taaacgaaga ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg
     2161 agtttcgtct aaacgcctat cgcgcatggc tggagatgga agaaccgcac tggttgaaag
     2221 cgcactacga caagctgagt tatcaggatt acagctacta ctcagcacca tcgtgcggta
     2281 attgtgacga cacttgcgcg tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct
     2341 ttttaagtaa aggggtggag gcggcgtttg agcagttggg cgttcccgtg cgggaaggca
     2401 aagaggtggc ggtggatgcc attttcgact cagtttcggt tgccactact tatcgcgaaa
     2461 aactggcgga gcagggaatt attttctgtt cctttggtga ggcgatccac gatcacccgg
     2521 aactggtgcg taaatatctc ggcaccgtgg tgccggggaa tgacaacttc tttgccgcgc
     2581 ttaatgcggc ggtagcctct gatggtacgt ttatttatgt gcctaaaggc gtgcgctgcc
     2641 cgatggaact ttccacctat tttcgcatta acgcagaaaa aaccgggcag tttgagcgca
     2701 ccattctggt ggccgacgaa gacagctacg tcagctacat tgaaggctgt tccgctccgg
     2761 tgcgtgacag ctatcagtta cacgcggcag tggtggaagt catcatccat aaaaacgccg
     2821 aggtgaaata ttccacggta caaaactggt ttcctggcga taacaacacc ggcggtattc
     2881 tcaacttcgt caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc
     2941 aatcagaaac cgggtcagcg attacgtgga aatatcccag ctgcattttg cgcggcgata
     3001 actccattgg tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg
     3061 gcaccaagat gatccacatc ggtaaaaaca ccaaatcgac cattatctcg aaagggatct
     3121 ctgccggaca tagtcagaac agttatcgcg gcttagtgaa aatcatgccg acggcaacca
     3181 atgcgcgcaa tttcactcag tgcgactcaa tgctgattgg cgctaattgt ggggcgcata
     3241 ccttcccgta tgttgagtgt cgtaacaata gtgcgcaact ggaacacgag gcaacgacat
     3301 cacgtattgg tgaagatcaa ctgttttact gcctgcaacg cgggatcagc gaagaagacg
     3361 ccatctcgat gattgttaac ggtttctgca aagacgtgtt ctcggagctg ccgttggaat
     3421 ttgccgttga agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc ggataaggaa
     3481 taaacatgtt aagtattaaa gatttacacg tcagcgtgga agataaagct atcctgcgcg
     3541 gattaagcct cgacgttcat cccggcgaag ttcacgccat tatggggcca aacggttcgg
     3601 gcaaaagtac cttatcggca acgcttgccg ggcgagaaga ttatgaagtg acgggcggca
     3661 cggttgagtt caaaggcaaa gatttgcttg cgctgtcgcc ggaagatcgc gcgggcgaag
     3721 gcatctttat ggccttccag tatccggtgg agattccagg tgtcagtaac cagtttttcc
     3781 tgcaaacggc acttaatgcg gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg
     3841 attttcagga tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa gatttattaa
     3901 cccgttcggt aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa
     3961 tggcggtgct ggaaccggag ttatgcattc ttgatgagtc ggactccggg ctggatattg
     4021 acgcattaaa agtggtcgcc gatggcgtga actcgctgcg tgatggcaag cgctcattca
     4081 tcattgttac gcactaccaa cgcattctcg actacatcaa gcctgattac gttcatgtgc
     4141 tatatcaggg acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc
     4201 agggttatgg ctggcttacc gaacagcagt aacgcgctgc aacagtggca tcacttgttt
     4261 gaagctgaag ggacaaaacg ctccccgcaa gcacagcagc atttacaaca attgctgcgt
     4321 accggactgc cgacacgtaa acatgaaaac tggaaatata cgccgctgga agggctgatc
     4381 aatagccagt ttgtcagcat tgcgggagag atatccccac agcagcgtga tgccttagcg
     4441 ttaacgttag actccgtgcg gctggtgttt gtcgatgggc gttacgtgcc cgcactgagc
     4501 gatgcaactg aaggcagcgg atatgaagtg agcattaacg acgaccgtca gggtttaccc
     4561 gacgctattc aggcggaagt gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg
     4621 catatcgccg tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat gcatatcacc
     4681 cagggcgtgg caggtgaaga ggtgaacact gcccattacc gacatcatct ggatctggcg
     4741 gaaggtgccg aagcaacggt gatcgaacat tttgtcagcc tgaatgatgc tcgtcatttt
     4801 accggggcac ggttcactat caacgtcgca gcgaatgccc acttgcagca tatcaagctg
     4861 gcgtttgaaa acccgctcag tcaccacttt gctcataacg atttgttgct ggctgaggat
     4921 gccaccgcat ttagccacag tttcctgctg ggtggcgcag tgttacgaca caacaccagt
     4981 acgcaactca atggcgaaaa cagcacgctg cggatcaata gcctggcgat gccggtgaaa
     5041 aacgaggtgt gtgatacccg tacctggctg gaacacaata aaggtttttg taacagccga
     5101 cagttgcaca aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac
     5161 gtcgcgcagc acgccatcaa aacggatggt cagatgacca acaacaatct gctgatgggc
     5221 aaactggcgg aagtggatac gaaaccgcag ctggaaatct atgcagatga tgtgaaatgc
     5281 agccacggcg cgacggtggg gcgtattgat gatgaacaga tattctatct gcgctcgcgc
     5341 gggatcaatc agcaggatgc ccagcagatg atcatttacg ccttcgctgc cgaactgacg
     5401 gaagcactgc gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg tcaacggctg
     5461 ccaggaggtg caagatgatt ttttccgtca acaaagtgcg ggccgacttt ccggtgcttt
     5521 cgcgtgaggt aaacggtttg ccgctggctt atctcgacag cgccgccagt gcgcagaaac
     5581 cgagccaggt gattgacgcc gaggccgagt tttatcgtca tggctacgcg gcggtgcatc
     5641 gtggtattca taccttaagc gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg
     5701 catcgctgtt tattaatgcc cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg
     5761 aagggatcaa tctggtcgcc aatagctggg gcaacagcaa cgtgcgggcg ggcgataaca
     5821 tcatcatcag tcagatggag caccacgcta acattgttcc ctggcagatg ctttgcgcac
     5881 gcgttggcgc agagccgcgt gtgatcccgc tcaatcccga tggtacgtcg caactggaga
     5941 cgctgcctac gctgtttgat gagaaaactc gcctgctggc aattactcat gtctccaacg
     6001 tgcttggcac agaaaatcca ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa
     6061 aagtgctggt ggatggcgct caggcggtga tgcatcatcc ggtggatgtt caggcgctgg
     6121 attgcgactt ttacgtgttc tccgggcata aactgtatgg ccccaccgga attggcattc
     6181 tttatgtgaa agaagccttg ttgcaggaga tgccgccgtg ggaagggggc ggttctatga
     6241 tcgccaccgt cagcctgagt gaaggcacta cctggaccaa agcaccatgg cggtttgaag
     6301 ccggtacacc caataccggg ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg
     6361 cgctggggct taataacata gccgagtatg aacagaatct gatgcattat gcgctatcac
     6421 agctggaatc tgtaccggat ctcactctct atggcccaca aaacaggctt ggcgttattg
     6481 cttttaatct cggtaaacac cacgcctatg atgttggcag ttttctcgat aattacggca
     6541 ttgctgtgcg taccggacat cactgcgcaa tgccattgat ggcctactac aacgtccctg
     6601 cgatgtgtcg ggcgtcgctg gccatgtata acacccatga agaagtggat cgtctggtga
     6661 ccggcctgca acgtattcac cgtttgctgg gataacaggg aggcactatg gctttattgc
     6721 cggataaaga aaagttgctg cgtaattttt tacgctgcgc caactgggaa gagaaatatc
     6781 tctacattat tgagctgggc cagcgtctgc cagaattacg cgacgaagac agaagtccac
     6841 aaaatagcat tcaaggctgt cagagtcagg tgtggattgt catgcgccag aatgcccagg
     6901 gaattattga attacagggc gacagcgatg cggcgattgt gaaagggctt attgcggtcg
     6961 tctttattct ctacgatcag atgacgccgc agggtattgt caatttcgat gtgcgtccgt
     7021 ggtttgaaaa aatggcgctc acccaacatc tcaccccatc tcgttcacaa ggtctggaag
     7081 cgatgattcg cgcaattcgc gccaaagccg ctgcacttag ctaaaccaaa aggacagctt
     7141 tcatccggtt ggctcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
     7201 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     7261 ctggctcacc ttcgggtggg cctttctgcg tttataccta gggatatatt ccgcttcctc
     7321 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     7381 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     7441 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     7501 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     7561 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     7621 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     7681 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     7741 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     7801 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     7861 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     7921 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     7981 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     8041 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgttac
     8101 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     8161 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa
     8221 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8281 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8341 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8401 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8461 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8521 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8581 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8641 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8701 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8761 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8821 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8881 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8941 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9001 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9061 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9121 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9181 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct
//
