LOCUS       Hy_pRnrS_eGFP_SV40_#9                 8205 bp    DNA     circular SYN 21-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             join(8063..8205,1..883)
                     /note="pLannotate"
                     /label="HygR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5292..6152)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2804..3523
                     /note="pLannotate"
                     /label="EGFP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    1886..2007
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    3532..3653
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(6153..6257)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          1246..1311
                     /note="pLannotate"
                     /label="small t intron"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     rep_origin      complement(4534..5121)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        7738..8017
                     /note="pLannotate"
                     /label="copia promoter"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1441..1461
                     /note="pLannotate"
                     /label="SV40 NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3678..3979
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="25.4"
                     /fragment="True"
                     /other="CDS"
     CDS             907..1185
                     /note="pLannotate"
                     /label="yokD (fragment)"
                     /database="swissprot"
                     /identity="52.7"
                     /match_length="34.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(2768..2800)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="30.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1780..1863
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4221..4349)
                     /note="pLannotate"
                     /label="YPB1_ECOLX (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="32.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
       61 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
      121 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
      181 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
      241 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
      301 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
      361 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
      421 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
      481 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
      541 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
      601 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
      661 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
      721 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
      781 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
      841 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat gccgaccgaa
      901 caagagctga tttcgagaac gcctcagcca gcaactcgcg cgagcctagc aaggcaaatg
      961 cgagagaacg gccttacgct tggtggcaca gttctcgtcc acagttcgct aagctcgctc
     1021 ggctgggtcg cgggagggcc ggtcgcagtg attcaggccc ttctggattg tgttggtccc
     1081 cagggcacga ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc agattggaga
     1141 tcgccgcccg tgcctgccga ttgggtgcag atctttgtga aggaacctta cttctgtggt
     1201 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     1261 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     1321 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     1381 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     1441 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     1501 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     1561 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     1621 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     1681 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     1741 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     1801 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     1861 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     1921 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1981 tttgtccaaa ctcatcaatg tatcttatca tgtctggatt aattaacgat taaacgtact
     2041 tatcgataaa tatcaacagc cagctgttat gttattgtac tgttatttac atgattcgct
     2101 gtaatggaat tcattcaaag ttgttgaatt gagtatgtta gcacctgttg catttccaaa
     2161 taattcgaga tttccaaaaa aaaaattaat tttaagtcaa tgtccttgga aaaaaatttt
     2221 aactttgttt caccttgatt ttttatctaa ttgtagcaag aggaccaaat tttttcactc
     2281 aaaactaatt aagtggcgtt tcatcagctg ttttctggat tagtctaggg ttgtctcatt
     2341 gcatgaaata tcgatgataa aaaaatttca aaatttattt agtatttgaa actattaata
     2401 ttaatatttt tcaagtgaca agctgggaag ctaaacataa aattgtgcag taaggattcg
     2461 atttatggtt taagaaaaga aaactaccac cccataattg cattagattt accctaaatt
     2521 tataaaaagt gaattgacgc actcgacagc cctgattttc ccatagtttt cccatcacca
     2581 aaaatggcgg caaatcgaaa cagtttgccg cccggcataa aacccaatgt agctgtattt
     2641 cccagatcat ttgccacaca actttcaaac tgtacactta atacacgtcg tggtttaagt
     2701 gaattttacc agagaatcag agaagcgccc ctacctgcta ataataatcc attcaaaaac
     2761 atctcaactc gaggtcgacg gtatcgataa gcttgatatc accatggtga gcaagggcga
     2821 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2881 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2941 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3001 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3061 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3121 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3181 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3241 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3301 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3361 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3421 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3481 cgccgccggg atcactctcg gcatggacga gctgtacaag taagcggccg caacttgttt
     3541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaactagtg
     3661 gatccactag gggccgccga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     3721 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     3781 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg
     3841 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     3901 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     3961 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     4021 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     4081 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt
     4141 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     4201 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     4261 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     4321 tcgtaaagtc tggaaacgcg gaagtcagcg ctcttccgct tcctcgctca ctgactcgct
     4381 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4441 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4501 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4561 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4621 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4681 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4741 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4801 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4861 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4921 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     4981 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5041 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5101 gcgcagaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5281 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5341 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5401 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5461 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5521 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5581 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     5641 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5701 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5761 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5821 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5881 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     5941 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6001 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6061 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6121 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6181 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6241 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     6301 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     6361 ttcacatttg tacgaatttt ttttttatca aaagttcgag tttttcacca atttcctcat
     6421 caaccgagca aggcaaacgg ctttgaataa tatggtgtta tatatacata tatcaaatcg
     6481 ctgctgactg cgtgattgat ggccccaaga ttacatatta tcgaatcagg attcagaagg
     6541 agatcaatgt caaatgcgga caggaacatg aaagacgcct gttatgcgca attaaaaatt
     6601 tgggtttaat tgctgtggaa actgttgttg gcggcatctt aagttcctgt ttaacaacat
     6661 caactactta tgtacgtaga agcgtttaag ccatttgcat acagatgaga actggctttt
     6721 gtgctaatca gtcaagatga ctccgatgat gatgactcat tacctgacca gttttcgctg
     6781 ctttcttttc aacaactact tgtatatgta ttgtatccaa tagcaataca ttgaatttcc
     6841 atggtctagt cacgtattat catttaattg acaccaagtc gtgttattgt tgagctatcg
     6901 agttcagctc aaacatttct tattcccatg aataagccgg caaaaatatg caatctatga
     6961 aagttaatat aagcaaacct tactttgact caataccaat gcactttgtg tcgataggtt
     7021 cacgcaattg aggcgattat tccgataacc caagcgattg actgttcccg tttcgattcc
     7081 aattgaaatt tggaaatgta caatagtttt gctatatgct gtcaagtacg ctcttatctt
     7141 ctctgggttt tcttcagagt ttcgaaacgc ttcttctttt ttttgttttt tttttttgga
     7201 atctcgtatt ttggaagggg ctcccctctg gaatttgtta cactgtcgtt atcattgcga
     7261 acaagcggcc cgaagctatc agcgacttta acatttacaa tgcacttttt tacgaccaat
     7321 taaatgtaca ttttcctttc ttcgcccgtt gataagcgaa cgcgatgtgg cgcaggcaat
     7381 gtgttgctct tgcgacacaa acgcaatcaa aatggattca atttcgcttt ttcccagtga
     7441 aacgaagaac gaaccgacca tcatgatatg ctcctctgca tgttgcgtat tgaatcaatg
     7501 acaatttcaa ttaagccgcc cgttcgtcat gcgttttcgt gcgcttcgaa atgctgataa
     7561 cgctgctgtc ctccaactgc tttgcatgtg gacacaattc catttattta attcttttat
     7621 ttggatcggt taaattaaaa agcgccttgt tacgcattta acgttgtttc cggtgcgtgg
     7681 tggtttcatg cttctgggaa cggcaaatgg gtttaggatt gggaacccct catcatctgt
     7741 tggaatatac tattcaacct acaaaagtaa cgttaaacaa cactacttta tatttgatat
     7801 gaatggccac accttttatg ccataaaaca tattgtaaga gaataccact ctttttattc
     7861 cttctttcct tcttgtacgt tttttgctgt aagtaggtcg tggtgctggt gttgcagttg
     7921 aaataactta aaatataaat cataaaactc aaacataaac ttgactattt atttatttat
     7981 taagaaagga aatataaatt ataaattaca acaggttatg gacctgcagc caagcttggc
     8041 gctcgtccgg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     8101 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     8161 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcct
//
