LOCUS       Exported               11756 bp ds-DNA     circular SYN 09-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 1, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        128..339
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      359..368
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             368..391
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             398..418
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             443..4543
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4544..4591
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4607..4663
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4664..5377
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    5396..5984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5867..5878)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6056..6289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6367..6488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6528..6663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6684..6702)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6712..6728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6870..7325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7351..7455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7456..8316
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8487..9075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9363..9384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9399..9429
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9437..9453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9461..9477
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9498..9516
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9544..9770
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9771..9951
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9998..10123
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10616..10849
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11007..11026
                     /label=7q Centro 1
     promoter        complement(11027..11318)
                     /label=bU6 Promoter
                     /note="Bos taurus U6 snRNA U6-2 promoter regionACCESSION 
                     DQ150531"
     misc_feature    11422..11441
                     /label=7p Centro 1
     promoter        complement(11451..11691)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    join(11698..11756,1..59)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
       61 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagtgg ctccggtgcc
      121 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      181 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      241 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      301 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg cgggatccgc
      361 caccatggat tacaaagacg atgacgataa gatggcccca aagaagaagc ggaaggtcgg
      421 tatccacgga gtcccagcag ccgacaagaa gtacagcatc ggcctggaca tcggcaccaa
      481 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
      541 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
      601 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
      661 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
      721 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
      781 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
      841 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
      901 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
      961 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     1021 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     1081 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     1141 gaatggcctg ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     1201 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     1261 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     1321 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     1381 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     1441 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     1501 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     1561 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     1621 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     1681 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     1741 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     1801 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     1861 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     1921 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     1981 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     2041 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa
     2101 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     2161 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     2221 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     2281 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     2341 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     2401 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     2461 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     2521 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     2581 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     2641 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     2701 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     2761 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     2821 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     2881 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     2941 cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa
     3001 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     3061 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
     3121 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     3181 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     3241 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
     3301 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     3361 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     3421 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     3481 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     3541 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     3601 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg
     3661 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat
     3721 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     3781 gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     3841 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     3901 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     3961 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     4021 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     4081 ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     4141 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     4201 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
     4261 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
     4321 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
     4381 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     4441 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     4501 cctgtacgag acacggatcg acctgtctca gctgggaggc gacaagcgtc ctgctgctac
     4561 taagaaagct ggtcaagcta agaaaaagaa agctagcggc agcggcgcca ccaacttcag
     4621 cctgctgaag caggccggcg acgtggagga gaaccccggc cccgtgagca agggcgagga
     4681 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4741 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4801 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4861 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4921 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4981 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5041 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5101 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5161 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5221 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5281 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5341 cgccgggatc actctcggca tggacgagct gtacaagtta gcgcgttaag tcgacaatca
     5401 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5461 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5521 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5581 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5641 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5701 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5761 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5821 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5881 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5941 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     6001 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6061 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     6301 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6361 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6421 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6481 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6541 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6601 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6661 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     6721 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6781 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6841 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     6901 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6961 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7021 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7081 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7141 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7201 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7261 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     7321 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7381 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7441 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7501 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7561 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7621 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7681 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7741 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7801 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7861 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7921 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7981 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8041 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8101 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8161 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8221 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8281 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8341 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9181 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9241 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9301 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     9361 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     9421 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     9481 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     9541 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     9601 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     9661 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     9721 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     9781 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9841 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9901 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     9961 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    10021 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    10081 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    10141 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    10201 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    10261 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10321 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10381 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10441 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    10501 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    10561 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    10621 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    10681 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    10741 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    10801 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    10861 gttgctctgg aaaactcgcc atttgtctca agatctagaa ttcaaaaaaa gcaccgactc
    10921 ggtgccactt tgctgtttcc agcaaagttg ataacggact agccttattt aaacttgcta
    10981 tgctgtttcc agcatagctc ttaaacattg gcttccaatt gaatcacgat aaacatggcc
    11041 tctcaagaac tatataaagc taagaataga aaaaatttta cagttatggt gactaaactg
    11101 taaccctttt ttaagtttca aagtatataa taagttatga ccttttgtac cacacacatt
    11161 atcatcatat agtttgatta gggctaagtt taatagtctc cataacctta ttacatcata
    11221 tatgcaaatg aagggaacca tgggaaattg gccctaggcc cgcccgacct ctgcgcgtct
    11281 gtctagtgga ctgcgcgggc agacaagctc ggtcacgcaa aaaaagcacc gactcggtgc
    11341 cactttgctg tttccagcaa agttgataac ggactagcct tatttcaact tgctatgcac
    11401 tcttgtgcat agctctgaaa ccaggtctgt tggagtttac tcggtgtttc gtcctttcca
    11461 caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc
    11521 cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca
    11581 cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta tctctctaac
    11641 agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct cccacaattt
    11701 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataa
//
