LOCUS       Exported                4196 bp ds-DNA     circular SYN 20-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 1, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     412..427
                     /label=Telo Seq 1
     primer_bind     502..518
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    538..1545
                     /label=ArtificialTelomere
     primer_bind     1546..1570
                     /label=508 Telo  Proximal Sequencing (29)
     primer_bind     complement(1559..1575)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1559..1575
                     /label=KS Primer Seq (55)
     primer_bind     complement(1559..1574)
                     /label=Telo Seq 2 (KS Primer)
     primer_bind     1568..1604
                     /label=PuroR REV(2)
     CDS             complement(1588..2187)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1606..1622
                     /label=Puro Seq R (153)
     primer_bind     complement(2166..2207)
                     /label=PuroR FWD(2)
     primer_bind     2168..2208
                     /label=EF1a REV
     primer_bind     complement(2170..2187)
                     /label=Puro Seq F (152)
     promoter        complement(2188..2399)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     2373..2387
                     /label=Telo Backbone F
     primer_bind     complement(2382..2425)
                     /label=EF1a FWD
     primer_bind     complement(2383..2399)
                     /label=EF-1a Sequencing (45)
     promoter        complement(2419..2437)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2447..2463)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2605..3060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3086..3190
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3086..3113
                     /label=AmpR  Promoter Sequencing (28)
     CDS             3191..4051
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3308..3329)
                     /label=AmpR Seq R (61)
     primer_bind     complement(4029..4048)
                     /label=Amp Seq F (147)
     primer_bind     complement(join(4176..4196,1))
                     /label=Telo Backbone R
ORIGIN
        1 tcgatgatat cagatccccg ggatgcagaa attgatgatc tattaaacaa taaagatgtc
       61 cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta
      121 cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc
      181 ctgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc
      241 ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat
      301 agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt tgtggttcta
      361 agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc agcagcctct
      421 gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca tcagaagctg
      481 gtcgactcta gatgcggccg ctctagaact agtggatccg tataccatga attcgatccc
      541 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      601 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      661 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      721 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      781 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      841 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      901 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      961 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1021 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1081 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1141 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1201 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1261 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1321 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1381 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1441 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
     1501 taaccctaac cctaacccta accctaaccc taaccctaac cctaaccatc aagcttatcg
     1561 ataccgtcga cctcgagggg gggcccgtca ggcaccgggc ttgcgggtca tgcaccaggt
     1621 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     1681 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     1741 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     1801 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     1861 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     1921 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     1981 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     2041 cagctcggtg acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta
     2101 gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag
     2161 gcgcaccgtg ggcttgtact cggtcatctg tgttctggcg gcaaacccgt tgcgaaaaag
     2221 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     2281 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt
     2341 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgcccg
     2401 gtaccgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     2461 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2521 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2581 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2641 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2701 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2761 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2821 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2881 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2941 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3001 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3061 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3121 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3181 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3241 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3301 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3361 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3421 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3481 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3541 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3601 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3661 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3721 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3781 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3841 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3901 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3961 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4021 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4081 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4141 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt catgca
//
