LOCUS       Exported               10648 bp ds-DNA     circular SYN 27-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLVx_Tet-On_Puro_MDM4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 1, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        9..376
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(37..56)
                     /label=Seq 4
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(353..402)
                     /label=MDM4 Dox Hifi 2F
     primer_bind     363..406
                     /label=MDM4 Dox Hifi 1F
     protein_bind    383..386
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             386..1858
                     /codon_start=1
                     /label=MDM4
                     /translation="MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGE
                     MFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRK
                     NLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEH
                     KCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQT
                     NQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKN
                     DDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHS
                     LSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAH
                     SSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLV
                     TCFHCARRLKKAGASCPICKKEIQLVIKVFIA"
     primer_bind     complement(647..667)
                     /label=Seq 3
     primer_bind     complement(1249..1268)
                     /label=Seq 2
     primer_bind     complement(1818..1878)
                     /label=MDM4 Dox Hifi 1R
     primer_bind     1839..1870
                     /label=MDM4 Dox HiFI 2R
     primer_bind     complement(1854..1875)
                     /label=Seq 1
     polyA_signal    2051..2185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2075..2093)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    2229..2344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     primer_bind     complement(2522..2546)
                     /label=Seq 20
     misc_feature    2836..3069
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     complement(3121..3140)
                     /label=Seq 19
     misc_feature    3566..3691
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     3719..3737
                     /label=Seq 18
     primer_bind     complement(3719..3737)
                     /label=Seq 17
     LTR             3738..4371
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4363..4387)
                     /label=Seq 16
     polyA_signal    4539..4673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4563..4582)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4674..4693)
                     /label=Seq 15
     promoter        4721..4825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4826..5686
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5044..5063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(5234..5253)
                     /label=Seq 14
     primer_bind     complement(5818..5837)
                     /label=Seq 13
     rep_origin      5857..6445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6346..6365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6362..6383)
                     /label=Seq 12
     primer_bind     6599..6616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6733..6754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6769..6799
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6807..6823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6812..6834
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6831..6847
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6831..6847
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6960..6982
                     /label=Seq 11
     primer_bind     complement(6960..6982)
                     /label=Seq 10
     LTR             6975..7608
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7681..7700)
                     /label=Seq 9
     misc_feature    7815..8403
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7921..7932
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8275..8292)
                     /label=Seq 8
     primer_bind     8330..8350
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8417..9016)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8500..8520)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8863..8882)
                     /label=Seq 7
     primer_bind     8997..9016
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(9025..9354)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9039..9174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9078..9089
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9093..9112)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             complement(9365..10111)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9460..9481)
                     /label=Seq 6
     primer_bind     complement(10069..10087)
                     /label=Seq 5
     promoter        complement(10130..10640)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10193..10211)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaagaat tcggcatgac atcattttcc acctctgctc agtgttcaac
      421 atctgacagt gcttgcagga tctctcctgg acaaatcaat caggtacgac caaaactgcc
      481 gcttttgaag attttgcatg cagcaggtgc gcaaggtgaa atgttcactg ttaaagaggt
      541 catgcactat ttaggtcagt acataatggt gaagcaactt tatgatcagc aggagcagca
      601 tatggtatat tgtggtggag atcttttggg agaactactg ggacgtcaga gcttctccgt
      661 gaaagaccca agccctctct atgatatgct aagaaagaat cttgtcactt tagccactgc
      721 tactacagat gctgctcaga ctctcgctct cgcacaggat cacagtatgg atattccaag
      781 tcaagaccaa ctgaagcaaa gtgcagagga aagttccact tccagaaaaa gaactacaga
      841 agacgatatc cccacactgc ctacctcaga gcataaatgc atacattcta gagaagatga
      901 agacttaatt gaaaatttag cccaagatga aacatctagg ctggaccttg gatttgagga
      961 gtgggatgta gctggcctgc cttggtggtt tttaggaaac ttgagaagca actatacacc
     1021 tagaagtaat ggctcaactg atttacagac aaatcaggat gtgggtactg ccattgtttc
     1081 agatactaca gatgacttgt ggtttttgaa tgagtcagta tcagagcagt taggtgttgg
     1141 aataaaagtt gaagctgctg atactgaaca aacaagtgaa gaagtaggga aagtaagtga
     1201 caaaaaggtg attgaagtgg gaaaaaatga tgacctggag gactctaagt ccttaagtga
     1261 tgataccgat gtagaggtta cctctgagga tgagtggcag tgtactgaat gcaagaaatt
     1321 taactctcca agcaagaggt actgttttcg ttgttgggcc ttgaggaagg attggtattc
     1381 agattgttca aagttaaccc attctctctc cacgtctgat atcactgcca tacctgaaaa
     1441 ggaaaatgaa ggaaatgatg tccctgattg tcgaagaacc atttcggctc ctgtcgttag
     1501 acctaaagat gcgtatataa agaaagaaaa ctccaaactt tttgatccct gcaactcagt
     1561 ggaattcttg gatttggctc acagttctga aagccaagag accatctcaa gcatgggaga
     1621 acagttagat aacctttctg aacagagaac agatacagaa aacatggagg attgccagaa
     1681 tctcttgaag ccatgtagct tatgtgagaa aagaccacga gacgggaaca ttattcatgg
     1741 aaggacgggc catcttgtca cttgttttca ctgtgccaga agactaaaga aggctggggc
     1801 ttcatgccct atttgcaaga aagagattca gctggttatt aaggttttta tagcataaac
     1861 cggtgccggc ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt
     1921 aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc
     1981 caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg
     2041 agggatttga gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
     2101 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
     2161 cattataagc tgcaataaac aagttaagtc gataaactgg atctctgctg tccctgtaat
     2221 aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
     2281 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
     2341 taaaggcgat accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct
     2401 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
     2461 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     2521 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     2581 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     2641 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     2701 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     2761 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     2821 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     2881 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     2941 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     3001 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     3061 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     3121 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
     3181 ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg
     3241 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
     3301 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
     3361 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
     3421 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
     3481 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
     3541 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
     3601 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
     3661 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
     3721 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
     3781 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     3841 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     3901 gtctaaccag agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg
     3961 ctcgccactc cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc
     4021 ccttgtagca agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca
     4081 cgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat
     4141 ccatcccatg caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt
     4201 ctaccttatc tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc
     4261 tgacccctgg ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga
     4321 tccacagatc aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag
     4381 tttgtagtac atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca
     4441 caggtttaga gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga
     4501 agtttgggat gattttggtt agcttgagtt atccagttga tccagacatg ataagataca
     4561 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4621 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttgatctcc
     4681 cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4741 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4801 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4861 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4921 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4981 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5041 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5101 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5161 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5221 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5281 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5341 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5401 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5461 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5521 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5581 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5641 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5701 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5761 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5881 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5941 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6001 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6061 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6241 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6481 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6541 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6601 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6661 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6721 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     6781 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6841 ctatgaccat gattacgcca agctctagct agaggtcgac ggtaaacgct agcaatgtca
     6901 aggcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
     6961 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
     7021 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     7081 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     7141 taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc
     7201 gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg gaaagtccct
     7261 tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct cgggccacgt
     7321 gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc gggtcatcca
     7381 tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg gcctcttcta
     7441 ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga
     7501 cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg tggtagatcc
     7561 acagatcaag gatatcttgt cttcgttggg agtgaattag cccttccagt cccctctttt
     7621 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtacct
     7681 gaggtgtgac tggaaaaccc acctcctcct cctcttgtgc ttctagccag gcacaatcag
     7741 cattggtagc tgctgtattg ctacttgtga ttgctccatg tttttctagg tctcgactgc
     7801 agaattaatt ccaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     7861 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     7921 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     7981 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
     8041 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     8101 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     8161 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     8221 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     8281 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     8341 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     8401 attgttccag acgcgttcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
     8461 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
     8521 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
     8581 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
     8641 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
     8701 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
     8761 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga
     8821 cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga
     8881 cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggtag tcggcgaacg
     8941 cggcggcgag ggtgcgtacg gcccggggga cgtcgtcgcg ggtggcgagg cgcaccgtgg
     9001 gcttgtactc ggtcatggtc gcgttttgca aaagcctagg cctccaaaaa agcctcctca
     9061 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     9121 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     9181 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     9241 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     9301 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcgc
     9361 gcgtttaccc ggggagcatg tcaaggtcaa aatcgtcaag agcgtcagca ggcagcatat
     9421 caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg
     9481 cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt
     9541 ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtt
     9601 tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat
     9661 gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca
     9721 gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt
     9781 cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga
     9841 ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt
     9901 ccagggggca ggagtgggta tgatgcctgt ccagcatctc gattggcagg gcatcgagca
     9961 gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc agcttttgag
    10021 cgagtttcct tgtcgtcagg ccttcgatac cgactccatt gagtaattcc agagcagagt
    10081 ttatgacttt gctcttgtcc agtctagaca tggtaattcg atgatccccc tggggagaga
    10141 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
    10201 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
    10261 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
    10321 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
    10381 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
    10441 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
    10501 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
    10561 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
    10621 aaaggcgcaa ccccaacccc gtggaatt
//
