LOCUS       Exported                6018 bp ds-DNA     circular SYN 01-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 1, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    612..617
                     /label=XHO1
     misc_feature    618..640
                     /label=Coronin1C [*]
     misc_feature    638..656
                     /label=sirna target
     misc_feature    641
                     /label=Coronin1C [*] [*] [5']
     misc_feature    643
                     /label=Coronin1C [*] [*] [3'] [*]
     misc_feature    644
                     /label=Coronin1C [*] [*] [3'] [*] [5']
     misc_feature    646
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*]
     misc_feature    647
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [*]
     misc_feature    650
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [5']
     misc_feature    652
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [3'] [*]
     misc_feature    655
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [3'] [...
     misc_feature    656
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [3'] [...
     misc_feature    658..808
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [3'] [...
     misc_feature    806..825
                     /label=sirna target
     misc_feature    809
                     /label=Coronin1C [*] [*] [5']
     misc_feature    811
                     /label=Coronin1C [*] [*] [3'] [*]
     misc_feature    812
                     /label=Coronin1C [*] [*] [3'] [*] [5']
     misc_feature    814
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*]
     misc_feature    815
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [5']
     misc_feature    817
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [*]
     misc_feature    818
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [*] [5']
     misc_feature    820
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [*] [3...
     misc_feature    821
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [*] [3...
     misc_feature    823
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [*] [3...
     misc_feature    824
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [*] [3...
     misc_feature    826..1813
                     /label=Coronin1C [*] [*] [3'] [*] [3'] [*] [3'] [*] [3...
     misc_feature    1811..1832
                     /label=P3sirna
     misc_feature    1814
                     /label=Coronin1C [*] [*] [5']
     misc_feature    1816
                     /label=Coronin1C [*] [*] [3'] [*]
     misc_feature    1817
                     /label=Coronin1C [*] [*] [3'] [*] [5']
     misc_feature    1819
                     /label=Coronin1C [*] [*] [3'] [*] [3']
     misc_feature    1820
                     /label=Coronin1C [*] [*] [5']
     misc_feature    1822
                     /label=Coronin1C [*] [*] [3'] [*]
     misc_feature    1823
                     /label=Coronin1C [*] [*] [3'] [*] [5']
     misc_feature    1825
                     /label=Coronin1C [*] [*] [3'] [*] [3']
     misc_feature    1826
                     /label=Coronin1C [*] [*] [5']
     misc_feature    1828
                     /label=Coronin1C [*] [*] [3']
     misc_feature    1829
                     /label=Coronin1C [*] [*] [5']
     misc_feature    1831..1949
                     /label=Coronin1C [*] [*] [3']
     misc_feature    1952..1957
                     /label=Bamh1 [3']
                     /note="note=""direct strand 
                     translation""translation=""GS"""
     misc_feature    1968..2684
                     /label=GFP1
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agccatatat ggagttccgc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      541 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
      601 cggactcaga tctcgagatg aggcgagtgg tacgacagag taaattccgc cacgtgtttg
      661 ggcaagcggt gaaaaatgac cagtgctatg atgacatccg ggtttctcgt gtgacctggg
      721 atagttcctt ttgtgctgtc aatcccagat ttgttgccat aatcatagag gcaagtgggg
      781 gaggagcgtt ccttgtcctc cctctgcata aaaccgggcg catcgacaaa tcttacccta
      841 cagtatgtgg ccacacagga ccagtgctgg acatagactg gtgcccacat aacgatcagg
      901 tcattgccag cggttcagag gactgcacgg tcatggtatg gcagatccca gaaaatggac
      961 tcaccctttc cctgactgaa cctgtggtga ttttggaagg ccactcaaag agagtcggca
     1021 tcgtggcttg gcatccaacg gcccgcaatg tgcttcttag tgcaggctgt gataatgcca
     1081 ttatcatctg gaatgtggga acaggggaag cccttataaa cttggacgat atgcattcag
     1141 acatgattta caatgtgagc tggaaccgga atggcagtct gatctgcaca gcttccaaag
     1201 acaagaaagt gagagtcatt gatcccagga aacaagagat tgttgctgag aaggagaaag
     1261 cacatgaagg agcaagaccc atgagagcca tcttcctggc cgatggcaat gtcttcacca
     1321 ctgggttcag ccgcatgagc gagcggcagc tggctctctg gaatccgaaa aatatgcagg
     1381 aaccaattgc tcttcatgag atggacacta gcaatggggt gttgctgcct ttctatgacc
     1441 ctgacaccag catcatttac ttatgtggaa agggtgacag cagtattcgc tattttgaga
     1501 tcacggatga atccccgtac gtccactacc tcaacacatt cagcagcaag gagcctcaga
     1561 gagggatggg ttacatgccc aagaggggac ttgatgttaa caaatgtgag attgccagat
     1621 tcttcaaact tcatgagaga aagtgtgaac ctattattat gactgttccc aggaagtctg
     1681 accttttcca agatgacctg tatcctgaca cagcggggcc agaggccgcg ctggaggcag
     1741 aagagtggtt cgaaggcaag aatgcagacc caatcctcat ctccttgaag cacgggtaca
     1801 ttccaggcaa aaatagagac ctgaaagtcg tcaagaagaa cattctggat agcaagccca
     1861 ctgcaaacaa gaagtgcgac ctgatcagca tccccaagaa aaccacagac acggccagtg
     1921 tgcaaaatga agccaagttg gatgagattc gggatccacc ggtcatggtg agcaagggcg
     1981 ccgagctgtt caccggcatc gtgcccatcc tgatcgagct gaatggcgat gtgaatggcc
     2041 acaagttcag cgtgagcggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2101 agttcatctg caccaccggc aagctgcctg tgccctggcc caccctggtg accaccctga
     2161 gctacggcgt gcagtgcttc tcacgctacc ccgatcacat gaagcagcac gacttcttca
     2221 agagcgccat gcctgagggc tacatccagg agcgcaccat cttcttcgag gatgacggca
     2281 actacaagtc gcgcgccgag gtgaagttcg agggcgatac cctggtgaat cgcatcgagc
     2341 tgaccggcac cgatttcaag gaggatggca acatcctggg caataagatg gagtacaact
     2401 acaacgccca caatgtgtac atcatgaccg acaaggccaa gaatggcatc aaggtgaact
     2461 tcaagatccg ccacaacatc gaggatggca gcgtgcagct ggccgaccac taccagcaga
     2521 atacccccat cggcgatggc cctgtgctgc tgcccgataa ccactacctg tccacccaga
     2581 gcgccctgtc caaggacccc aacgagaagc gcgatcacat gatctacttc ggcttcgtga
     2641 ccgccgccgc catcacccac ggcatggatg agctgtacaa gtgagcggcc gcgactctag
     2701 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2761 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     2941 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3001 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3061 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3121 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3181 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3241 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3301 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3361 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3481 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     3541 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3601 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3661 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3721 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3781 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3841 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     3901 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3961 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4021 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4081 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4141 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4201 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4261 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4321 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4381 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4441 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4501 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4561 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4621 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4681 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4741 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4801 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4861 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     4921 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     4981 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     5041 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     5101 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     5161 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5221 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5281 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5341 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5401 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5461 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5521 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5581 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5641 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5701 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5761 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5821 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5881 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5941 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6001 cgtattaccg ccatgcat
//
