LOCUS       Exported                6730 bp ds-DNA     circular SYN 01-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 1, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pmNeonGreen-N1 Vector.xdna [4693  nt] : (SacII[649] / 
            HindIII[622]) to ABHD16Acyto.txt.xdna [1332  nt] : (HindIII[7] / 
            SacII[1342])
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    520..589
                     /label=CMV Promoter
     misc_feature    628..630
                     /label=start
     misc_feature    631..1329
                     /label=Stim1TMD
     misc_feature    1330..2655
                     /label=ABHD16Acyto
     misc_feature    1981..1986
                     /label=Kozak
     CDS             1987..2027
                     /label=mNeonGreen [3'] [5']
     CDS             2727..3396
                     /label=mNeonGreen [3'] [3']
     rep_origin      3656..4096
                     /direction=RIGHT
                     /label=F1 ori
     rep_origin      3656..4096
                     /direction=RIGHT
                     /label=F1 ori
     promoter        4163..4191
                     /label=Amp prom
     promoter        4163..4191
                     /label=Amp prom
     promoter        4395..4591
                     /label=SV40 early promoter
     promoter        4395..4591
                     /label=SV40 early promoter
     misc_feature    4442..4519
                     /label=SV40 ORI
     misc_feature    4442..4519
                     /label=SV40 ORI
     CDS             4629..5420
                     /label=Kan/neoR
     rep_origin      6006..6634
                     /direction=RIGHT
                     /label=ColE1 origin
     rep_origin      6006..6634
                     /direction=RIGHT
                     /label=ColE1 origin
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttatg gatgtatgcg tccgtcttgc cctgtggctc
      661 ctctggggac tcctcctgca ccagggccag agcctcagcc atagtcacag tgagaaggcg
      721 acaggaacca gctcgggggc caactctgag gagtccactg cagcagagtt ttgccgaatt
      781 gacaagcccc tgtgtcacag tgaggatgag aaactcagct tcgaggcagt ccgtaacatc
      841 cacaaactga tggacgatga tgccaatggt gatgtggatg tggaagaaag tgatgagttc
      901 ctgagggaag acctcaatta ccatgaccca acagtgaaac acagcacctt ccatggtgag
      961 gataagctca tcagcgtgga ggacctgtgg aaggcatgga agtcatcaga agtatacaat
     1021 tggaccgtgg atgaggtggt acagtggctg atcacatatg tggagctgcc tcagtatgag
     1081 gagaccttcc ggaagctgca gctcagtggc catgccatgc caaggctggc tgtcaccaac
     1141 accaccatga cagggactgt gctgaagatg acagaccgga gtcatcggca gaagctgcag
     1201 ctgaaggctc tggatacagt gctctttggg cctcctctct tgactcgcca taatcacctc
     1261 aaggacttca tgctggtggt gtctatcgtt attggtgtgg gcggctgctg gtttgcctat
     1321 atccagaacg gccgctggac caacccccag taccggcagt tcatcaccat cttggaagca
     1381 acacatcgga accagtcttc agaaaacaag aggcagcttg ccaactacaa ctttgacttc
     1441 cggagctggc cagtcgactt ccactgggaa gaacccagca gccggaagga gtctcgaggg
     1501 ggcccttccc gccggggtgt ggccctgctt cgcccagagc ccctgcaccg ggggacagca
     1561 gacaccctcc tcaaccgggt taagaagctg ccttgtcaga tcaccagcta cctggtggcg
     1621 cacaccctag ggcgccggat gctgtatcca ggctctgtgt acctgctgca gaaggccctc
     1681 atgcctgtgc tgctgcaggg ccaggcccga ctggtggaag agtgtaatgg gcgccgggca
     1741 aagctgctgg cctgtgatgg caatgagatt gacaccatgt ttgtggaccg gcgggggaca
     1801 gctgagcccc agggacagaa gctggtgatc tgctgtgagg ggaatgctgg gttttatgag
     1861 gtgggctgcg tctccacgcc cctggaagct ggatattcag tcctgggctg gaatcatcca
     1921 ggctttgctg gaagcacggg ggtgccattc ccgcagaatg aggctaatgc catggatgtg
     1981 gtggtccagt ttgccatcca ccgcctaggc ttccagcccc aggacatcat catctacgcc
     2041 tggtccatcg gcggcttcac tgccacgtgg gcagccatgt cctacccaga tgttagtgcc
     2101 atgatcctgg atgcctcctt tgatgacctg gtgcccttgg ccttgaaggt catgccagac
     2161 agctggaggg gcctggtgac caggaccgtg aggcagcatc tcaatctaaa caacgcggag
     2221 cagctgtgca gataccaggg tcctgtactg ctgatccgga gaaccaagga tgagatcatc
     2281 accaccacgg ttcctgagga catcatgtcc aaccgaggca atgacctcct gctgaagctc
     2341 ctgcagcatc ggtatccccg ggtgatggca gaggagggtc ttcgagtggt gaggcagtgg
     2401 ttggaggcct cctcacagct ggaggaagcc tcaatttata gccgatggga ggtggaagag
     2461 gactggtgtc tgtctgtcct ccgctcctac caggcagaac acgggcccga cttcccctgg
     2521 agcgtggggg aggacatgag tgcagatgga cggcggcagc tggctttgtt tctggctcgg
     2581 aagcatctgc acaactttga ggccactcac tgcaccccac tcccagccca gaacttccag
     2641 atgccctggc acctcccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc
     2701 gaggaggata acatggcctc tctcccagcg acacatgagt tacacatctt tggctccatc
     2761 aacggtgtgg actttgacat ggtgggtcag ggcaccggca atccaaatga tggttatgag
     2821 gagttaaacc tgaagtccac caagggtgac ctccagttct ccccctggat tctggtccct
     2881 catatcgggt atggcttcca tcagtacctg ccctaccctg acgggatgtc gcctttccag
     2941 gccgccatgg tagatggctc cggctaccaa gtccatcgca caatgcagtt tgaagatggt
     3001 gcctccctta ctgttaacta ccgctacacc tacgagggaa gccacatcaa aggagaggcc
     3061 caggtgaagg ggactggttt ccctgctgac ggtcctgtga tgaccaactc gctgaccgct
     3121 gcggactggt gcaggtcgaa gaagacttac cccaacgaca aaaccatcat cagtaccttt
     3181 aagtggagtt acaccactgg aaatggcaag cgctaccgga gcactgcgcg gaccacctac
     3241 acctttgcca agccaatggc ggctaactat ctgaagaacc agccgatgta cgtgttccgt
     3301 aagacggagc tcaagcactc caagaccgag ctcaacttca aggagtggca aaaggccttt
     3361 accgatgtga tgggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat
     3421 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3481 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     3661 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3721 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3781 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3841 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3901 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3961 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     4021 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     4081 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4201 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     4261 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4321 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4381 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4441 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4501 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4561 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     4621 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4681 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4741 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4801 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4861 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4921 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4981 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5041 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5101 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     5161 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5221 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5281 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5341 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5401 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5461 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5521 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5581 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     5641 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     5701 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     5761 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     5821 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     5881 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5941 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6001 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6061 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6721 cgccatgcat
//
