LOCUS       Exported                6367 bp ds-DNA     circular SYN 01-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 1, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Ligation of : pAcmCherry-N1 Vector.xdna [4684  nt] : (SacII[649] / 
            HindIII[622]) to ABHD16A-mNG.txt.xdna [1677  nt] : (HindIII[622] / 
            SacII[2302])
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    520..589
                     /label=CMV Promoter
                     627..2303
                     /note="note=""Fragment extracted from ABHD16A-mNG.txt.xdna 
                     [1677  nt] : (HindIII[622] / SacII[2302])"
     misc_feature    628..2301
                     /label=ABHD16A
     misc_feature    628..975
                     /label=New Feature
                     /note="note=""direct strand 
                     translation""translation=""MAKLLSCVLGPRLYKIYRERDSERAPASVPET
                     PTAVTAPHSSSWDTYYQPRALEKHADSILALASVFWSISYYSSPFAFFYLYRKGYLSLS
                     KVVPFSHYAGTLLLLLAGVACLRGI"""
     misc_feature    896..914
                     /label=siRNA #9 targeted
     misc_feature    1147..1149
                     /label=G174 lipase like motif
                     /note="note=""direct strand translation""translation=""G"""
     misc_feature    1153..1155
                     /label=S176 lipase like motif
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    1162..1164
                     /label=G179 lipase like motif
                     /note="note=""direct strand translation""translation=""G"""
     misc_feature    1192..1194
                     /label=H189A
     misc_feature    1207..1209
                     /label=D194N
     misc_feature    1537..1539
                     /label=G304 lipase like motif 2
                     /note="note=""direct strand translation""translation=""G"""
     misc_feature    1543..1545
                     /label=S306 lipase like motif 2
                     /note="note=""direct strand translation""translation=""S"""
     misc_feature    1552..1554
                     /label=G309 lipase like motif 2
                     /note="note=""direct strand translation""translation=""G"""
     misc_feature    1653
                     /label=wobble mutation (A>G)
     misc_feature    1690..1692
                     /label=S355A
     misc_feature    1915..1917
                     /label=D430N
                     /note="note=""direct strand translation""translation=""D"""
     misc_feature    2146..2148
                     /label=H507A
                     /note="note=""direct strand translation""translation=""H"""
     misc_feature    2154
                     /label=wobble mutation (C>T)
     misc_feature    2163
                     /label=wobble?
     misc_feature    2326..3033
                     /label=pmCherry
                     /note="note=""Fragment extracted from Sequence Window #6 
                     [711  nt] : (BamHI[1] / NotI[715])"
     rep_origin      3293..3733
                     /direction=RIGHT
                     /label=F1 ori
     rep_origin      3293..3733
                     /direction=RIGHT
                     /label=F1 ori
     promoter        3800..3828
                     /label=Amp prom
     promoter        3800..3828
                     /label=Amp prom
     promoter        4032..4228
                     /label=SV40 early promoter
     promoter        4032..4228
                     /label=SV40 early promoter
     misc_feature    4079..4156
                     /label=SV40 ORI
     misc_feature    4079..4156
                     /label=SV40 ORI
     CDS             4266..5057
                     /label=Kan/neoR
     rep_origin      5643..6271
                     /direction=RIGHT
                     /label=ColE1 origin
     rep_origin      5643..6271
                     /direction=RIGHT
                     /label=ColE1 origin
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttatg gcgaagctgc tgagctgcgt cctaggcccc
      661 cggctctaca aaatctaccg ggagagggac tctgaaaggg ccccggccag cgtccctgag
      721 acgccaacgg cagtcactgc cccccattcc agctcctggg atacgtacta tcagccccgt
      781 gccctggaga aacatgctga cagcatcctg gcactggctt cagtattctg gtccatctct
      841 tattactcct ctcccttcgc cttcttctac ttgtacagga aaggttactt gagtttaagt
      901 aaggtagtac cattttctca ctatgctggg acattgctgc tacttctggc aggtgtggcc
      961 tgcctccgag gcattggccg ctggaccaac ccccagtacc ggcagttcat caccatcttg
     1021 gaagcaacac atcggaacca gtcttcagaa aacaagaggc agcttgccaa ctacaacttt
     1081 gacttccgga gctggccagt cgacttccac tgggaagaac ccagcagccg gaaggagtct
     1141 cgagggggcc cttcccgccg gggtgtggcc ctgcttcgcc cagagcccct gcaccggggg
     1201 acagcagaca ccctcctcaa ccgggttaag aagctgcctt gtcagatcac cagctacctg
     1261 gtggcgcaca ccctagggcg ccggatgctg tatccaggct ctgtgtacct gctgcagaag
     1321 gccctcatgc ctgtgctgct gcagggccag gcccgactgg tggaagagtg taatgggcgc
     1381 cgggcaaagc tgctggcctg tgatggcaat gagattgaca ccatgtttgt ggaccggcgg
     1441 gggacagctg agccccaggg acagaagctg gtgatctgct gtgaggggaa tgctgggttt
     1501 tatgaggtgg gctgcgtctc cacgcccctg gaagctggat attcagtcct gggctggaat
     1561 catccaggct ttgctggaag cacgggggtg ccattcccgc agaatgaggc taatgccatg
     1621 gatgtggtgg tccagtttgc catccaccgc ctaggcttcc agccccagga catcatcatc
     1681 tacgcctggt ccatcggcgg cttcactgcc acgtgggcag ccatgtccta cccagatgtt
     1741 agtgccatga tcctggatgc ctcctttgat gacctggtgc ccttggcctt gaaggtcatg
     1801 ccagacagct ggaggggcct ggtgaccagg accgtgaggc agcatctcaa tctaaacaac
     1861 gcggagcagc tgtgcagata ccagggtcct gtactgctga tccggagaac caaggatgag
     1921 atcatcacca ccacggttcc tgaggacatc atgtccaacc gaggcaatga cctcctgctg
     1981 aagctcctgc agcatcggta tccccgggtg atggcagagg agggtcttcg agtggtgagg
     2041 cagtggttgg aggcctcctc acagctggag gaagcctcaa tttatagccg atgggaggtg
     2101 gaagaggact ggtgtctgtc tgtcctccgc tcctaccagg cagaacacgg gcccgacttc
     2161 ccctggagcg tgggggagga catgagtgca gatggacggc ggcagctggc tttgtttctg
     2221 gctcggaagc atctgcacaa ctttgaggcc actcactgca ccccactccc agcccagaac
     2281 ttccagatgc cctggcacct cccgcgggcc cgggatccac cggtcatggt gagcaagggc
     2341 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     2401 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2461 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2521 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2581 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2641 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     2701 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     2761 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2821 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2881 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2941 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     3001 cactccaccg gcggcatgga cgagctgtac aaggcggccg cgactctaga tcataatcag
     3061 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3121 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3181 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3241 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     3301 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3361 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3421 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3481 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3541 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3601 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3661 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3721 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3841 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3901 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3961 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4021 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4081 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4141 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4201 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4261 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4321 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4381 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4441 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4501 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4561 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4621 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4681 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4741 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4801 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4861 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4921 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4981 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5041 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5101 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5161 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5221 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5281 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5341 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5401 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5461 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6301 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6361 catgcat
//
