LOCUS       PX458-tRNA_Gln-e        9112 bp DNA     circular SYN 02-DEC-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Thorben Huth
  TITLE     Direct Submission
  JOURNAL   Exported Dec 2, 2022 from SnapGene Viewer 6.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..78
                     /label=tRNA_Gln promoter
                     /note="color: #ffffff; direction: RIGHT"
     misc_RNA        97..172
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     enhancer        269..554
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
                     /note="color: #ffffff"
     promoter        556..833
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          834..1062
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
                     /note="color: #------"
     regulatory      1074..1083
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             1083..1148
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 1148"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1155..1175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             1200..5300
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /note="This feature has 7 segments:
                        1: 1200 .. 3737 / #993366
                        2: 3738 .. 3740 / #cc99b2
                        3: 3741 .. 4202 / #993366
                        4: 4203 .. 4205 / #cc99b2
                        5: 4206 .. 4373 / #993366
                        6: 4374 .. 4376 / #cc99b2
                        7: 4377 .. 5300 / #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5301..5348
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5364..5417
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 5414"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5418..6131
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="This feature has 2 segments:
                        1: 5418 .. 5420 / #05fc14 / 1a
                        2: 5421 .. 6131 / #05fc14"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             5418..6131
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 5418 .. 5420 / #05fd14 / 1a
                        2: 5421 .. 6131 / #05fd14"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    6165..6372
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   6381..6521
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      6596..7051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7333..7437
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7438..8298
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7438 .. 7506 / #ccffcc / signal sequence
                        2: 7507 .. 8298 / #ccffcc
                     Cleavage site after base 7506"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8469..9057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 acatgtggtt ccatggtgta atggttagca ctctggactc tgaatccagc gatccgagtt
       61 caaatctcgg tggaacctgg gtcttcgaga agacctgttt tagagctaga aatagcaagt
      121 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt
      181 tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat
      241 tctgcagaca aatggctcta gaggtacccg ttacataact tacggtaaat ggcccgcctg
      301 gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc
      361 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      421 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      481 gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      541 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
      601 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
      661 cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
      721 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      781 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
      841 ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      901 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
      961 tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg
     1021 tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca
     1081 tggactataa ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg
     1141 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
     1201 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
     1261 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
     1321 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
     1381 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
     1441 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
     1501 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
     1561 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
     1621 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
     1681 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     1741 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     1801 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     1861 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
     1921 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     1981 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     2041 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     2101 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     2161 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     2221 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct
     2281 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     2341 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     2401 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     2461 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     2521 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     2581 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     2641 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     2701 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     2761 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     2821 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     2881 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     2941 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     3001 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     3061 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     3121 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     3181 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     3241 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     3301 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     3361 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga
     3421 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg
     3481 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     3541 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     3601 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata
     3661 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg
     3721 tgcctcagag ctttctggcg gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     3781 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     3841 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     3901 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg
     3961 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     4021 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     4081 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     4141 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     4201 ctgcgctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     4261 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     4321 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aaggcgcctc
     4381 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     4441 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     4501 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     4561 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     4621 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     4681 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     4741 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     4801 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga
     4861 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     4921 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     4981 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     5041 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     5101 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     5161 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     5221 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     5281 tgtctcagct gggaggcgac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa
     5341 agaaaaagga attcggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg
     5401 aggagaatcc tggcccagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     5461 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     5521 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     5581 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     5641 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     5701 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5761 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5821 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     5881 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     5941 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6001 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     6061 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     6121 agctgtacaa ggaattctaa ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     6181 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6241 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6301 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag
     6361 gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     6421 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     6481 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     6541 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     6601 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6661 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6721 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6781 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     6841 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6901 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     6961 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7021 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     7081 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     7141 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     7201 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     7261 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7321 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7381 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7441 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7501 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7561 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7621 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7681 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7741 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7801 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7861 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7921 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7981 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8041 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8101 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc
     8161 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8221 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8281 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8341 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8401 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8461 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8521 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8581 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8641 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8701 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8761 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8821 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8881 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8941 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9001 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9061 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tc
//
