LOCUS       Exported               11756 bp ds-DNA     circular SYN 02-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Donglai
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 2, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1468..1487
                     /label=gRNA 2
     promoter        complement(1488..1779)
                     /label=bU6 Promoter
     misc_feature    1883..1902
                     /label=gRNA 1
     promoter        complement(1912..2152)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2159..2276
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2345..2556
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2585..2608
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2615..2635
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2660..6760
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6761..6808
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6824..6880
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6881..7594
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    7613..8201
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8084..8095)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8273..8506
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8584..8705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8745..8880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8901..8919)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8929..8945)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9087..9542
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9568..9672
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9673..10533
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10704..11292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11580..11601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11616..11646
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11654..11670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11678..11694
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11715..11733
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcgc catttgtctc aagatctaga attcaaaaaa agcaccgact
     1381 cggtgccact ttgctgtttc cagcaaagtt gataacggac tagccttatt taaacttgct
     1441 atgctgtttc cagcatagct cttaaactca cgtgattgtg gcatcatcga taaacatggc
     1501 ctctcaagaa ctatataaag ctaagaatag aaaaaatttt acagttatgg tgactaaact
     1561 gtaacccttt tttaagtttc aaagtatata ataagttatg accttttgta ccacacacat
     1621 tatcatcata tagtttgatt agggctaagt ttaatagtct ccataacctt attacatcat
     1681 atatgcaaat gaagggaacc atgggaaatt ggccctaggc ccgcccgacc tctgcgcgtc
     1741 tgtctagtgg actgcgcggg cagacaagct cggtcacgca aaaaaagcac cgactcggtg
     1801 ccactttgct gtttccagca aagttgataa cggactagcc ttatttcaac ttgctatgca
     1861 ctcttgtgca tagctctgaa accctggtca ttaggattcc gccggtgttt cgtcctttcc
     1921 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
     1981 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
     2041 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa
     2101 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcccacaatt
     2161 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2221 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2281 tttattacag ggacagcaga gatccacttt ggcgccggct cgagtggctc cggtgcccgt
     2341 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2401 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2461 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2521 gttctttttc gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg gatccgccac
     2581 catggattac aaagacgatg acgataagat ggccccaaag aagaagcgga aggtcggtat
     2641 ccacggagtc ccagcagccg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     2701 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     2761 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     2821 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     2881 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     2941 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     3001 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     3061 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     3121 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     3181 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     3241 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     3301 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     3361 tggcctgttc ggcaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     3421 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     3481 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     3541 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     3601 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     3661 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     3721 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     3781 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     3841 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     3901 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     3961 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     4021 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     4081 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     4141 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     4201 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     4261 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     4321 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     4381 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     4441 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     4501 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     4561 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     4621 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     4681 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     4741 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     4801 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     4861 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     4921 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     4981 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     5041 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     5101 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     5161 ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     5221 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     5281 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     5341 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     5401 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     5461 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     5521 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     5581 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     5641 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     5701 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     5761 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     5821 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     5881 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     5941 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     6001 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     6061 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     6121 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     6181 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     6241 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     6301 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     6361 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     6421 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     6481 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     6541 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     6601 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     6661 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     6721 gtacgagaca cggatcgacc tgtctcagct gggaggcgac aagcgtcctg ctgctactaa
     6781 gaaagctggt caagctaaga aaaagaaagc tagcggcagc ggcgccacca acttcagcct
     6841 gctgaagcag gccggcgacg tggaggagaa ccccggcccc gtgagcaagg gcgaggagct
     6901 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     6961 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     7021 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     7081 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     7141 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     7201 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     7261 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     7321 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     7381 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     7441 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     7501 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     7561 cgggatcact ctcggcatgg acgagctgta caagttagcg cgttaagtcg acaatcaacc
     7621 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7681 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7741 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7801 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7861 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7921 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7981 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     8041 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     8101 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     8161 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     8221 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8281 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     8341 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8401 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8461 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     8521 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8581 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8641 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8701 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     8761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8821 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8881 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     8941 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9001 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9061 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     9121 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9181 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9241 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9301 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9361 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9421 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9481 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9541 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9601 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9661 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9721 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9781 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9841 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9901 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9961 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10021 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10081 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10141 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10201 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10261 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10321 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10381 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10441 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10501 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10561 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10621 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10681 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10741 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10801 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10861 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10921 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10981 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11041 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11101 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11161 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11221 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11281 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11341 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11401 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11461 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11521 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    11581 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    11641 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    11701 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
