LOCUS       Exported               10797 bp ds-DNA     circular SYN 12-DEC-2022
DEFINITION  Mammalian bidirectional promoter vector for constitutively 
            expressing two genes at similar levels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 13, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     The gene inserted into each MCS should contain start and stop 
            codons.
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     source          1859..2755
                     /organism="synthetic"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      608..619
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             620..643
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             650..670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             677..1747
                     /codon_start=1
                     /label=Csm5
                     /translation="MKNDYRTFKLSLLTLAPIHIGNGEKYTSREFIYENKKFYFPDMGK
                     FYNKMVEKRLAEKFEAFLIQTRPNARNNRLISFLNDNRIAERSFGGYSISETGLESDKN
                     PNSAGAINEVNKFIRDAFGNPYIPGSSLKGAIRTILMNTTPKWNNENAVNDFGRFPKEN
                     KNLIPWGPKKGKEYDDLFNAIRVSDSKPFDNKSLILVQKWDYSAKTNKAKPLPLYRESI
                     SPLTKIEFEITTTTDEAGRLIEELGKRAQAFYKDYKAFFLSEFPDDKIQANLQYPIYLG
                     AGSGAWTKTLFKQADGILQRRYSRMKTKMVKKGVLKLTKAPLKTVKIPSGNHSLVKNHE
                     SFYEMGKANFMIKEIDK"
     CDS             1748..1801
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1802..1825
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1832..1852
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1859..2755
                     /codon_start=1
                     /gene="Csm4_ATUM"
                     /label=Csm4
                     /label=Csm4_ATUM
                     /translation="MTYKLYIMTFQNAHFGSGTLDSSKLTFSADRIFSALVLEALKMGK
                     LDAFLAEANQDKFTLTDAFPFQFGPFLPKPIGYPKHDQIDQSVDVKEVRRQAKLSKKLQ
                     FLALENVDDYLNGELFENEEHAVIDTVTKNQPHKDDNLYQVATTRFSNDTSLYVIANES
                     DLLNELMSSLQYSGLGGKRSSGFGRFELDIQNIPLELSDRLTKNHSDKVMSLTTALPVD
                     ADLEEAMEDGHYLLTKSSGFAFSHATNENYRKQDLYKFASGSTFSKTFEGQIVDVRPLD
                     FPHAVLNYAKPLFFKLEV"
     CDS             2756..2809
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2810..2833
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2840..2860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2867..3526
                     /codon_start=1
                     /label=Csm3
                     /translation="MTFAKIKFSAQIRLETGLHIGGSDAFAAIGAIDSPVIKDPITNLP
                     IIPGSSLKGKMRTLLAKVYNEKVAEKPSDDSDILSRLFGNSKDKRFKMGRLIFRDAFLS
                     NADELDSLGVRSYTEVKFENTIDRITAEANPRQIERAIRNSTFDFELIYEITDENENQV
                     EEDFKVIRDGLKLLELDYLGGSGSRGYGKVAFENLKATTVFGNYDVKTLNELLTAEV"
     CDS             3527..3580
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3581..3604
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3611..3631
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3638..4015
                     /codon_start=1
                     /label=Csm2
                     /translation="MTILTDENYVDIAEKAILKLERNTRNRKNPDAFFLTTSKLRNLLS
                     LTSTLFDESKVKEYDALLDRIAYLRVQFVYQAGREIAVKDLIEKAQILEALKEIKDRET
                     LQRFCRYMEALVAYFKFYGGKD"
     polyA_signal    4170..4251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4379..4627
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    4628..4663
                     /label=CRISPR Repeat
     misc_feature    4664..4693
                     /label=Spacer
     misc_feature    4694..4723
                     /label=Terminal CRISPR Repeat
     misc_feature    4724..4729
                     /label=Terminator
     rep_origin      complement(4791..5379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5550..6410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6411..6515)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6548..6629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6746..9019)
                     /codon_start=1
                     /label=Csm1
                     /translation="MKKEKIDLFYGALLHDIGKVIQRATGERKKHALVGADWFDEIADN
                     QVISDQIRYHMANYQSDKLGNDHLAYITYIADNIASGVDRRQSNEESDEDTSAKIWDTY
                     TNQADIFNVFGAQTDKRYFKPTVLNLKSKPNFASATYEPFSKGDYAAIATRIKNELAEF
                     EFNQVQIDSLLNLFEATLSFVPSSTNTKEIADISLADHSRLTAAFALAIYDYLEDKGRH
                     NYKEDLFTKVSAFYEEEAFLLASFDLSGIQDFIYNINIATNGAAKQLKARSLYLDFMSE
                     YIADSLLDKLGLNRANMLYVGGGHAYFVLANTEKTVETLVQFEKDFNQFLLANFQTRLY
                     VAFGWGSFAAKDIMSELNSPESYRQVYQKASRMISKKKISRYDYQTLMLLNRGGKSSER
                     ECEICHSVENLVSYHDQKVCDICRGLYQFSKEIAHDHFIITENEGLPIGPNACLKGVAF
                     EKLSQEAFSRVYVKNDYKAGTVKATHVFVGDYQCDEIYNYAALSKNENGLGIKRLAVVR
                     LDVDDLGAAFMAGFSQQGNGQYSTLSRSATFSRSMSLFFKVYINQFASDKKLSIIYAGG
                     DDVFAIGSWQDIIAFTVELRENFIKWTNGKLTLSAGIGLFADKTPISLMAHQTGELEEA
                     AKGNEKDSISLFSSDYTFKFDRFITNVYDDKLEQIRYFFNHQDERGKNFIYKLIELLRN
                     HDRMNMARLAYYLTRLEELTRETDRDKFKTFKNLFYSWYTNKNDKDRKEAELALLLYIY
                     EIRKD"
     CDS             complement(9026..9046)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9053..9076)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(9083..9100)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(9101..9154)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(9155..9883)
                     /codon_start=1
                     /label=Cas6
                     /translation="MKKLVFTFKRIDHPAQDLAVKFHGFLMEQLDSDYVDYLHQQQTNP
                     YATKVIQGKENTQWVVHLLTDDIEDKVFMTLLQIKEVSLNDLPKLSVEKVEIQELGADK
                     LLEIFNSEENQTYFSIIFETPTGFKSQGSYVIFPSMRLIFQSLMQKYGRLVENQPEIEE
                     DTLDYLSEHSTITNYRLETSYFRVHRQRIPAFRGKLTFKVQGAQTLKAYVKMLLTFGEY
                     SGLGMKTSLGMGGIKLEERKD"
     CDS             complement(9890..9910)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9917..9940)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(9941..9994)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(9995..10702)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     regulatory      10703..10711
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(10752..10789)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gggatccgcc gccaccatgg attacaaaga cgatgacgat aagatggcgc ctaagaagaa
      661 acgcaaagtg cggggcatga aaaatgacta ccggaccttc aagctgagcc tgctgaccct
      721 ggctcctatc cacatcggca acggcgagaa gtacaccagc agagaattca tctacgagaa
      781 caagaagttc tacttccccg acatgggcaa gttctacaac aagatggtgg aaaagagact
      841 ggccgagaag ttcgaggcct tcctgatcca gaccagaccc aacgccagaa acaaccggct
      901 gatttctttt ctgaacgaca acagaatcgc cgaaagatct tttggcggct acagcatcag
      961 tgaaaccggc ctggaatctg ataagaaccc taacagcgcc ggagctatca acgaggtgaa
     1021 caaattcatc cgggacgcct tcggaaatcc ttacatccca ggcagcagcc tgaagggcgc
     1081 catccgcacc atcctgatga acaccacacc taagtggaac aacgagaacg ccgtgaacga
     1141 cttcggcaga ttcccaaagg aaaacaagaa cctgatccct tggggaccta agaaaggcaa
     1201 ggaatacgac gacctgttca acgccatcag agtgtccgac agcaagccct tcgacaacaa
     1261 aagcctgatc ctcgtgcaga agtgggacta cagcgccaaa accaacaagg ccaagcctct
     1321 gcctctgtac agagagtcta tcagccctct gaccaagatc gagttcgaga taacaacaac
     1381 cactgatgag gccggcagac tgatcgagga actgggaaag cgggcccagg ccttttataa
     1441 ggactacaag gcctttttcc tgtctgaatt ccctgatgat aagatccagg ctaatctgca
     1501 ataccccatc tacctgggcg ccggcagcgg cgcttggaca aagaccctgt ttaagcaggc
     1561 cgacggcatc ctgcagcgga gatactccag aatgaaaacc aagatggtca agaagggcgt
     1621 gctgaagctg acaaaggccc ctctgaaaac agtgaagatc cccagcggca accacagcct
     1681 ggtgaagaat cacgagagct tctacgagat gggcaaagcc aacttcatga tcaaggaaat
     1741 cgacaaggaa ggaaggggct ccctcctcac ttgtggagat gtcgaagaaa atcctggacc
     1801 tgattacaaa gacgatgacg ataagatggc gcctaagaag aaacgcaaag tgcggggcat
     1861 gacttacaag ctctacatta tgacctttca aaacgcccac ttcggttccg gcactctgga
     1921 ctcatcgaag ctgaccttct ccgcggatag aatcttctcg gcactcgtgc tcgaggctct
     1981 gaagatggga aagctcgacg ccttcttggc cgaggccaac caggataagt tcactctgac
     2041 cgacgcgttc ccattccaat tcggtccttt cctgccgaaa ccgattggtt accccaagca
     2101 cgaccagatc gaccagtctg tggacgtgaa ggaagtccgc cgccaagcga agctgtccaa
     2161 aaagctccag ttcctggctc tggaaaacgt cgacgactac ctgaacggag agctgtttga
     2221 gaatgaggaa cacgccgtga tcgacacagt gaccaagaac cagccccata aagatgataa
     2281 tctgtaccaa gtggccacca ctcggttctc gaacgacacc tccctttacg tgatcgccaa
     2341 cgaatccgat ctgctgaacg aactgatgag cagccttcag tactccgggc tgggcggcaa
     2401 aaggtcctca ggattcggca gatttgagct ggacatccag aacattccct tggaactgtc
     2461 cgaccggctg acgaagaacc acagcgacaa ggtcatgtca cttaccaccg ccctcccggt
     2521 ggacgctgat ctcgaggaag cgatggaaga tggccattac ctgttgacca agtcgtccgg
     2581 attcgcattc tcccacgcca ccaacgaaaa ctatcggaag caggacctgt acaagttcgc
     2641 ctccgggagc accttcagca agactttcga gggacagatc gtggacgtgc gccctctcga
     2701 tttccctcac gccgtgctga actacgccaa gccgctgttc tttaagctcg aagtcgaggg
     2761 cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggacctg attacaaaga
     2821 cgatgacgat aagatggcgc ctaagaagaa acgcaaagtg cggggcatga ccttcgccaa
     2881 gatcaaattc agcgcccaga tccggctgga aaccggcctg cacatcggag gatctgatgc
     2941 ctttgccgct atcggcgcca tcgacagccc tgtgatcaag gaccccatca ccaacctgcc
     3001 tatcatcccc ggctctagcc tgaagggcaa gatgagaaca ctgctggcca aggtgtacaa
     3061 cgaaaaggtg gccgagaagc ctagcgacga cagcgacatc ctgagcagac tgttcggaaa
     3121 tagcaaggat aagcggttca agatgggcag actgatcttc cgggacgcct tcctgagcaa
     3181 cgccgacgag ctggattctc tgggcgtgcg gagctacacc gaggtgaagt tcgagaacac
     3241 catcgataga atcaccgccg aggccaatcc tagacagatc gagagagcca ttcggaactc
     3301 aacattcgac ttcgagctga tctacgagat cactgatgag aatgagaacc aggtcgagga
     3361 agatttcaag gtgatcagag acggcctgaa gctgctggaa ctggactacc tgggcggaag
     3421 cggctccaga ggctacggca aagtggcttt tgagaacctg aaagccacca cagtgttcgg
     3481 caactacgac gtgaaaaccc tgaacgagct gctgaccgcc gaagtggagg ggcgggggtc
     3541 tttgttgact tgcggggatg ttgaggagaa cccagggcca gattacaaag acgatgacga
     3601 taagatggcg cctaagaaga aacgcaaagt gcggggcatg accatcctga ccgacgagaa
     3661 ctacgtggac atcgccgaga aagccatcct gaagctggaa agaaacacca gaaatagaaa
     3721 gaaccctgat gccttcttcc tgaccacatc taagctgcgg aacctgctga gcctgacaag
     3781 caccctgttc gacgagagca aggtgaagga atacgacgcc ctgctggaca gaatcgctta
     3841 tctgagagtg cagttcgtgt accaggccgg cagagagatc gccgtgaaag atctgatcga
     3901 gaaggcccag atcctggaag ctctgaaaga gatcaaggac cgggaaaccc tgcagagatt
     3961 ctgcagatac atggaagccc tggtggccta cttcaagttc tacggcggca aggactgagc
     4021 tagcgcggcc gcatcgataa gcttgtcgac gatatctcca gaggatcata atcagccata
     4081 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     4141 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     4201 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg ccccgagctt
     4261 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4321 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aaactagtga
     4381 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     4441 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     4501 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     4561 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     4621 aaacaccgat ataaacctaa ttacctcgag aggggacgga aacccgtctt cgatgaagcg
     4681 attcagaaga cttgatataa acctaattac ctcgagaggg gactttttta catgtgagca
     4741 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4801 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4861 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4921 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4981 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5041 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5101 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5161 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5221 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5281 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5341 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5401 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5461 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5521 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5581 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5641 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5701 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5761 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5821 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5881 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5941 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6001 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6061 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6121 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     6181 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6241 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6301 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6361 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6421 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6481 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6541 gacgtcggca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6601 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6661 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6721 tggctgatta tgatcctcta gattaatcct ttctgatttc gtagatgtac agcaggagcg
     6781 cgagctcggc ttccttccgg tccttatcgt tcttgttggt gtaccaggag tagaacaggt
     6841 tcttgaaggt cttgaacttg tccctgtcag tctcccgggt cagttcttcc aggcgagtga
     6901 ggtaataggc gaggcgtgcc atattcatgc ggtcgtggtt cctcaaaagc tcaataagct
     6961 tatagatgaa gttctttccc ctctcgtctt gatggttgaa gaagtatcta atctgttcca
     7021 gtttgtcgtc gtacacgtta gtgatgaacc tatcaaactt gaaagtgtag tcgcttgaga
     7081 acagcgagat ggagtccttt tcgttgccct tggcggcctc ttccagttcc ccggtctggt
     7141 gagccatcag gctaatagga gtcttgtcgg cgaacaaccc tatccccgcg gagagggtga
     7201 gcttcccgtt ggtccacttg atgaagtttt cgcgaagttc cacagtgaac gcgatgatat
     7261 cctgccacga tccaatggcg aacacgtcat cgccgcccgc gtagataatg ctcagcttct
     7321 tgtcggaggc gaactggtta atgtacactt tgaagaacag cgacatgctc cgggagaatg
     7381 tggccgatct tgacagagtg gagtattgtc cgtttccctg ctggctgaaa ccggccatga
     7441 aggcggcgcc caagtcatcc acgtcgagcc ggaccacggc cagtctcttg atgcctaggc
     7501 cgttctcgtt cttgctcagg gcggcgtagt tgtagatctc gtcgcactgg taatccccca
     7561 cgaacacatg cgtagccttc acggtaccgg ccttatagtc attcttcacg tagacccggc
     7621 tgaacgcttc ttgggacagc ttttcgaatg ccacgccctt taagcacgcg tttggtccaa
     7681 tcggcagccc ctcattttcg gtaatgatga agtggtcatg ggcaatttct ttcgagaact
     7741 ggtacagtcc ccggcaaatg tcacagactt tctggtcgtg gtaggacacc aggttctcca
     7801 cggagtggca aatctcgcac tctctctctg aggactttcc accgcgattc aggagcatca
     7861 gtgtctggta gtcgtatctg gagattttct ttttggagat catgcgcgaa gccttttggt
     7921 acacttggcg gtaggactcg gggctattca gctcggacat gatgtccttg gccgcgaagg
     7981 aaccccagcc aaaggccaca tagaggcggg tctggaagtt tgccaacagg aactggttga
     8041 aatccttctc aaactgcacc agggtttcca cagtcttttc ggtgttggcc aggacgaagt
     8101 aggcgtgtcc gccgccgacg taaagcatgt tagccctgtt cagtcccagc ttgtccagca
     8161 ggctatcggc gatgtactcg gacataaagt ccaggtagag gctccgggcc ttcagctgct
     8221 tcgccgcgcc gttagttgcg atgttaatgt tgtagataaa gtcctggatt cccgacaggt
     8281 cgaaggaggc cagcaggaag gcttcttctt catagaacgc tgacactttg gtgaacaggt
     8341 cctctttgta gttgtgccga cccttgtcct ccaggtagtc gtagatcgcc agagcgaagg
     8401 cagcagtcaa gcgggaatgg tcggccaggg agatgtcggc gatttcctta gtgttggtgc
     8461 ttgacggcac gaaggagaga gtagcctcga aaaggttgag cagggagtca atctggactt
     8521 ggttgaactc gaactcggcc agttcgttct taatccgggt ggcgatggca gcgtaatcgc
     8581 ccttgctaaa gggttcgtaa gtggcggacg cgaagttggg cttcgacttg agattcagca
     8641 cggtaggctt gaagtaccgc ttatcggtct gcgctccgaa cacgttaaag atgtcggcct
     8701 ggttcgtgta ggtgtcccag atctttgcgg aggtatcttc gtctgactct tcgttggatt
     8761 gccggcggtc gacaccggag gcgatgttgt cggcaatgta ggtgatgtaa gccaggtgat
     8821 cgttgccgag cttatcagac tggtagttgg ccatatggta ccggatctga tccgagatga
     8881 cttggttgtc ggcgatctcg tcgaaccagt cggcgcccac aagtgcgtgt ttcttccgct
     8941 ctccggttgc tcgctggatg acctttccga tgtcgtgcag cagggctccg taaaacagat
     9001 caatcttttc tttcttcatg ccccgcactt tgcgtttctt cttaggcgcc atcttatcgt
     9061 catcgtcttt gtaatcgccg gaatggtgat ggtgatggtg tggtcctgga ttttcttcaa
     9121 catctccaca agttagcaaa cttcctcttc cttcgtcctt tctttcttcc agcttgatgc
     9181 cgcccattcc caggctggtt ttcatgccca ggccgctgta ctcgccgaag gtcagcagca
     9241 tcttcacgta ggccttcagt gtctgggctc cctgcacctt gaaggtcagc ttgccccgga
     9301 aggcggggat tctctgtcta tgcactctga agtagcttgt ttccagtctg taattggtga
     9361 tggtgctgtg ctcgctcagg tagtccaggg tatcttcctc gatctcaggc tggttttcca
     9421 ccagtctgcc gtacttctgc atcaggctct gaaagatcag ccgcatgctg gggaagatca
     9481 cgtagctgcc ctggctttta aagcctgtag gtgtctcgaa gatgatgctg aagtaggtct
     9541 ggttttcctc gctgttgaag atctcgagca gcttatcagc gcccagctcc tggatttcca
     9601 ccttttccac agacaactta ggcaggtcgt tcagggacac ttccttgatc tgcagcaggg
     9661 tcatgaacac cttgtcctcg atgtcgtctg tcagcagatg cacgacccac tgggtgttct
     9721 ctttgccctg gatcaccttt gtggcgtagg ggttggtctg ctgctggtgc aggtagtcca
     9781 cgtagtcgct atccagctgt tccatcagga agccgtggaa tttcacggcc aggtcctgag
     9841 cagggtggtc gatccgctta aaggtgaaca cgagcttttt catgccccgc actttgcgtt
     9901 tcttcttagg cgccatctta tcgtcatcgt ctttgtaatc ggggccgggg ttctcctcca
     9961 cgtcgccgca ggtcagcagg ctgcctctgc cctccttgta cagctcgtcc atgccgccgg
    10021 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
    10081 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
    10141 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
    10201 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
    10261 agcccatggt tttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
    10321 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
    10381 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
    10441 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
    10501 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
    10561 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
    10621 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
    10681 cctcctcgcc cttgctcacc atggtggcgg caccggtgaa ttctccaggc gatctgacgg
    10741 ttcactaaac gagctctgct tatataggcc tcccaccgta cacgccacct cgacata
//
