LOCUS       Exported               11591 bp ds-DNA     circular SYN 12-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   David Colognori
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 13, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    232..243
                     /label=Kozak sequence
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             268..291
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             298..318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             325..2601
                     /codon_start=1
                     /label=Csm1
                     /translation="MKKEKIDLFYGALLHDIGKVIQRATGERKKHALVGADWFDEIADN
                     QVISDQIRYHMANYQSDKLGNDHLAYITYIADNIASGVDRRQSNEESDEDTSAKIWDTY
                     TNQADIFNVFGAQTDKRYFKPTVLNLKSKPNFASATYEPFSKGDYAAIATRIKNELAEF
                     EFNQVQIDSLLNLFEATLSFVPSSTNTKEIADISLADHSRLTAAFALAIYDYLEDKGRH
                     NYKEDLFTKVSAFYEEEAFLLASFDLSGIQDFIYNINIATNGAAKQLKARSLYLDFMSE
                     YIADSLLDKLGLNRANMLYVGGGHAYFVLANTEKTVETLVQFEKDFNQFLLANFQTRLY
                     VAFGWGSFAAKDIMSELNSPESYRQVYQKASRMISKKKISRYDYQTLMLLNRGGKSSER
                     ECEICHSVENLVSYHDQKVCDICRGLYQFSKEIAHDHFIITENEGLPIGPNACLKGVAF
                     EKLSQEAFSRVYVKNDYKAGTVKATHVFVGDYQCDEIYNYAALSKNENGLGIKRLAVVR
                     LDVDDLGAAFMAGFSQQGNGQYSTLSRSATFSRSMSLFFKVYINQFASDKKLSIIYAGG
                     DDVFAIGSWQDIIAFTVELRENFIKWTNGKLTLSAGIGLFADKTPISLMAHQTGELEEA
                     AKGNEKDSISLFSSDYTFKFDRFITNVYDDKLEQIRYFFNHQDERGKNFIYKLIELLRN
                     HDRMNMARLAYYLTRLEELTRETDRDKFKTFKNLFYSWYTNKNDKDRKEAELALLLYIY
                     EIRKD"
     polyA_signal    2602..2649
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2680..2883
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2911..2922
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2923..2946
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2953..2973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2980..3360
                     /codon_start=1
                     /label=Csm2
                     /translation="MTILTDENYVDIAEKAILKLERNTRNRKNPDAFFLTTSKLRNLLS
                     LTSTLFDESKVKEYDALLDRIAYLRVQFVYQAGREIAVKDLIEKAQILEALKEIKDRET
                     LQRFCRYMEALVAYFKFYGGKD"
     polyA_signal    3361..3408
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3439..3642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3670..3681
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3682..3705
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3712..3732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3739..4401
                     /codon_start=1
                     /label=Csm3
                     /translation="MTFAKIKFSAQIRLETGLHIGGSDAFAAIGAIDSPVIKDPITNLP
                     IIPGSSLKGKMRTLLAKVYNEKVAEKPSDDSDILSRLFGNSKDKRFKMGRLIFRDAFLS
                     NADELDSLGVRSYTEVKFENTIDRITAEANPRQIERAIRNSTFDFELIYEITDENENQV
                     EEDFKVIRDGLKLLELDYLGGSGSRGYGKVAFENLKATTVFGNYDVKTLNELLTAEV"
     polyA_signal    4402..4449
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4480..4683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4711..4722
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4723..4746
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4753..4773
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4780..5679
                     /codon_start=1
                     /label=Csm4
                     /translation="MTYKLYIMTFQNAHFGSGTLDSSKLTFSADRIFSALVLEALKMGK
                     LDAFLAEANQDKFTLTDAFPFQFGPFLPKPIGYPKHDQIDQSVDVKEVRRQAKLSKKLQ
                     FLALENVDDYLNGELFENEEHAVIDTVTKNQPHKDDNLYQVATTRFSNDTSLYVIANES
                     DLLNELMSSLQYSGLGGKRSSGFGRFELDIQNIPLELSDRLTKNHSDKVMSLTTALPVD
                     ADLEEAMEDGHYLLTKSSGFAFSHATNENYRKQDLYKFASGSTFSKTFEGQIVDVRPLD
                     FPHAVLNYAKPLFFKLEV"
     polyA_signal    5680..5727
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5758..5961
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5989..6000
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6001..6024
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6031..6051
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6058..7131
                     /codon_start=1
                     /label=Csm5
                     /translation="MKNDYRTFKLSLLTLAPIHIGNGEKYTSREFIYENKKFYFPDMGK
                     FYNKMVEKRLAEKFEAFLIQTRPNARNNRLISFLNDNRIAERSFGGYSISETGLESDKN
                     PNSAGAINEVNKFIRDAFGNPYIPGSSLKGAIRTILMNTTPKWNNENAVNDFGRFPKEN
                     KNLIPWGPKKGKEYDDLFNAIRVSDSKPFDNKSLILVQKWDYSAKTNKAKPLPLYRESI
                     SPLTKIEFEITTTTDEAGRLIEELGKRAQAFYKDYKAFFLSEFPDDKIQANLQYPIYLG
                     AGSGAWTKTLFKQADGILQRRYSRMKTKMVKKGVLKLTKAPLKTVKIPSGNHSLVKNHE
                     SFYEMGKANFMIKEIDK"
     polyA_signal    7132..7179
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7210..7413
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7441..7452
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7453..7476
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7483..7503
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7510..8241
                     /codon_start=1
                     /label=Cas6
                     /translation="MKKLVFTFKRIDHPAQDLAVKFHGFLMEQLDSDYVDYLHQQQTNP
                     YATKVIQGKENTQWVVHLLTDDIEDKVFMTLLQIKEVSLNDLPKLSVEKVEIQELGADK
                     LLEIFNSEENQTYFSIIFETPTGFKSQGSYVIFPSMRLIFQSLMQKYGRLVENQPEIEE
                     DTLDYLSEHSTITNYRLETSYFRVHRQRIPAFRGKLTFKVQGAQTLKAYVKMLLTFGEY
                     SGLGMKTSLGMGGIKLEERKD"
     polyA_signal    8242..8289
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        8320..8523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8551..8559
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             8560..9270
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    9271..9318
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9349..9597
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    9598..9633
                     /label=CRISPR Repeat
     misc_feature    9634..9663
                     /label=Spacer
     misc_feature    9664..9693
                     /label=Terminal CRISPR Repeat
     misc_feature    9694..9699
                     /label=Terminator
     promoter        9837..9941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9942..10802
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10973..11561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag agccgccacc
      241 atgcaccatc accatcacca ttccggcgat tacaaagacg atgacgataa gatggcgcct
      301 aagaagaaac gcaaagtgcg gggcatgaag aaagaaaaga ttgatctgtt ttacggagcc
      361 ctgctgcacg acatcggaaa ggtcatccag cgagcaaccg gagagcggaa gaaacacgca
      421 cttgtgggcg ccgactggtt cgacgagatc gccgacaacc aagtcatctc ggatcagatc
      481 cggtaccata tggccaacta ccagtctgat aagctcggca acgatcacct ggcttacatc
      541 acctacattg ccgacaacat cgcctccggt gtcgaccgcc ggcaatccaa cgaagagtca
      601 gacgaagata cctccgcaaa gatctgggac acctacacga accaggccga catctttaac
      661 gtgttcggag cgcagaccga taagcggtac ttcaagccta ccgtgctgaa tctcaagtcg
      721 aagcccaact tcgcgtccgc cacttacgaa ccctttagca agggcgatta cgctgccatc
      781 gccacccgga ttaagaacga actggccgag ttcgagttca accaagtcca gattgactcc
      841 ctgctcaacc ttttcgaggc tactctctcc ttcgtgccgt caagcaccaa cactaaggaa
      901 atcgccgaca tctccctggc cgaccattcc cgcttgactg ctgccttcgc tctggcgatc
      961 tacgactacc tggaggacaa gggtcggcac aactacaaag aggacctgtt caccaaagtg
     1021 tcagcgttct atgaagaaga agccttcctg ctggcctcct tcgacctgtc gggaatccag
     1081 gactttatct acaacattaa catcgcaact aacggcgcgg cgaagcagct gaaggcccgg
     1141 agcctctacc tggactttat gtccgagtac atcgccgata gcctgctgga caagctggga
     1201 ctgaacaggg ctaacatgct ttacgtcggc ggcggacacg cctacttcgt cctggccaac
     1261 accgaaaaga ctgtggaaac cctggtgcag tttgagaagg atttcaacca gttcctgttg
     1321 gcaaacttcc agacccgcct ctatgtggcc tttggctggg gttccttcgc ggccaaggac
     1381 atcatgtccg agctgaatag ccccgagtcc taccgccaag tgtaccaaaa ggcttcgcgc
     1441 atgatctcca aaaagaaaat ctccagatac gactaccaga cactgatgct cctgaatcgc
     1501 ggtggaaagt cctcagagag agagtgcgag atttgccact ccgtggagaa cctggtgtcc
     1561 taccacgacc agaaagtctg tgacatttgc cggggactgt accagttctc gaaagaaatt
     1621 gcccatgacc acttcatcat taccgaaaat gaggggctgc cgattggacc aaacgcgtgc
     1681 ttaaagggcg tggcattcga aaagctgtcc caagaagcgt tcagccgggt ctacgtgaag
     1741 aatgactata aggccggtac cgtgaaggct acgcatgtgt tcgtggggga ttaccagtgc
     1801 gacgagatct acaactacgc cgccctgagc aagaacgaga acggcctagg catcaagaga
     1861 ctggccgtgg tccggctcga cgtggatgac ttgggcgccg ccttcatggc cggtttcagc
     1921 cagcagggaa acggacaata ctccactctg tcaagatcgg ccacattctc ccggagcatg
     1981 tcgctgttct tcaaagtgta cattaaccag ttcgcctccg acaagaagct gagcattatc
     2041 tacgcgggcg gcgatgacgt gttcgccatt ggatcgtggc aggatatcat cgcgttcact
     2101 gtggaacttc gcgaaaactt catcaagtgg accaacggga agctcaccct ctccgcgggg
     2161 atagggttgt tcgccgacaa gactcctatt agcctgatgg ctcaccagac cggggaactg
     2221 gaagaggccg ccaagggcaa cgaaaaggac tccatctcgc tgttctcaag cgactacact
     2281 ttcaagtttg ataggttcat cactaacgtg tacgacgaca aactggaaca gattagatac
     2341 ttcttcaacc atcaagacga gaggggaaag aacttcatct ataagcttat tgagcttttg
     2401 aggaaccacg accgcatgaa tatggcacgc ctcgcctatt acctcactcg cctggaagaa
     2461 ctgacccggg agactgacag ggacaagttc aagaccttca agaacctgtt ctactcctgg
     2521 tacaccaaca agaacgataa ggaccggaag gaagccgagc tcgcgctcct gctgtacatc
     2581 tacgaaatca gaaaggatta acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     2641 cgtttgttcg cacacattag ctagccgtca gcacacattg tgatgcggtt ttggcagtac
     2701 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2761 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2821 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2881 gctcgtttag tgaaccgtca gatctctaga gccgccacca tggattacaa agacgatgac
     2941 gataagatgg cgcctaagaa gaaacgcaaa gtgcggggca tgaccatcct gaccgacgag
     3001 aactacgtgg acatcgccga gaaagccatc ctgaagctgg aaagaaacac cagaaataga
     3061 aagaaccctg atgccttctt cctgaccaca tctaagctgc ggaacctgct gagcctgaca
     3121 agcaccctgt tcgacgagag caaggtgaag gaatacgacg ccctgctgga cagaatcgct
     3181 tatctgagag tgcagttcgt gtaccaggcc ggcagagaga tcgccgtgaa agatctgatc
     3241 gagaaggccc agatcctgga agctctgaaa gagatcaagg accgggaaac cctgcagaga
     3301 ttctgcagat acatggaagc cctggtggcc tacttcaagt tctacggcgg caaggactga
     3361 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcag ttcttttgcc
     3421 ttactttcaa tgcatgcggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3541 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3601 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     3661 atctctagag ccgccaccat ggattacaaa gacgatgacg ataagatggc gcctaagaag
     3721 aaacgcaaag tgcggggcat gaccttcgcc aagatcaaat tcagcgccca gatccggctg
     3781 gaaaccggcc tgcacatcgg aggatctgat gcctttgccg ctatcggcgc catcgacagc
     3841 cctgtgatca aggaccccat caccaacctg cctatcatcc ccggctctag cctgaagggc
     3901 aagatgagaa cactgctggc caaggtgtac aacgaaaagg tggccgagaa gcctagcgac
     3961 gacagcgaca tcctgagcag actgttcgga aatagcaagg ataagcggtt caagatgggc
     4021 agactgatct tccgggacgc cttcctgagc aacgccgacg agctggattc tctgggcgtg
     4081 cggagctaca ccgaggtgaa gttcgagaac accatcgata gaatcaccgc cgaggccaat
     4141 cctagacaga tcgagagagc cattcggaac tcaacattcg acttcgagct gatctacgag
     4201 atcactgatg agaatgagaa ccaggtcgag gaagatttca aggtgatcag agacggcctg
     4261 aagctgctgg aactggacta cctgggcgga agcggctcca gaggctacgg caaagtggct
     4321 tttgagaacc tgaaagccac cacagtgttc ggcaactacg acgtgaaaac cctgaacgag
     4381 ctgctgaccg ccgaagtgtg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     4441 cgtttgttct ggattgcgag aatggactag tagcaaactg tgatgcggtt ttggcagtac
     4501 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4561 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     4621 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     4681 gctcgtttag tgaaccgtca gatctctaga gccgccacca tggattacaa agacgatgac
     4741 gataagatgg cgcctaagaa gaaacgcaaa gtgcggggca tgacttacaa gctctacatt
     4801 atgacctttc aaaacgccca cttcggttcc ggcactctgg actcatcgaa gctgaccttc
     4861 tccgcggata gaatcttctc ggcactcgtg ctcgaggctc tgaagatggg aaagctcgac
     4921 gccttcttgg ccgaggccaa ccaggataag ttcactctga ccgacgcgtt cccattccaa
     4981 ttcggtcctt tcctgccgaa accgattggt taccccaagc acgaccagat cgaccagtct
     5041 gtggacgtga aggaagtccg ccgccaagcg aagctgtcca aaaagctcca gttcctggct
     5101 ctggaaaacg tcgacgacta cctgaacgga gagctgtttg agaatgagga acacgccgtg
     5161 atcgacacag tgaccaagaa ccagccccat aaagatgata atctgtacca agtggccacc
     5221 actcggttct cgaacgacac ctccctttac gtgatcgcca acgaatccga tctgctgaac
     5281 gaactgatga gcagccttca gtactccggg ctgggcggca aaaggtcctc aggattcggc
     5341 agatttgagc tggacatcca gaacattccc ttggaactgt ccgaccggct gacgaagaac
     5401 cacagcgaca aggtcatgtc acttaccacc gccctcccgg tggacgctga tctcgaggaa
     5461 gcgatggaag atggccatta cctgttgacc aagtcgtccg gattcgcatt ctcccacgcc
     5521 accaacgaaa actatcggaa gcaggacctg tacaagttcg cctccgggag caccttcagc
     5581 aagactttcg agggacagat cgtggacgtg cgccctctcg atttccctca cgccgtgctg
     5641 aactacgcca agccgctgtt ctttaagctc gaagtctaac ggcaataaaa agacagaata
     5701 aaacgcacgg tgttgggtcg tttgttcgca cattcaaaaa caggcaattg gacaagcgtg
     5761 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     5821 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     5881 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     5941 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tctctagagc cgccaccatg
     6001 gattacaaag acgatgacga taagatggcg cctaagaaga aacgcaaagt gcggggcatg
     6061 aaaaatgact accggacctt caagctgagc ctgctgaccc tggctcctat ccacatcggc
     6121 aacggcgaga agtacaccag cagagaattc atctacgaga acaagaagtt ctacttcccc
     6181 gacatgggca agttctacaa caagatggtg gaaaagagac tggccgagaa gttcgaggcc
     6241 ttcctgatcc agaccagacc caacgccaga aacaaccggc tgatttcttt tctgaacgac
     6301 aacagaatcg ccgaaagatc ttttggcggc tacagcatca gtgaaaccgg cctggaatct
     6361 gataagaacc ctaacagcgc cggagctatc aacgaggtga acaaattcat ccgggacgcc
     6421 ttcggaaatc cttacatccc aggcagcagc ctgaagggcg ccatccgcac catcctgatg
     6481 aacaccacac ctaagtggaa caacgagaac gccgtgaacg acttcggcag attcccaaag
     6541 gaaaacaaga acctgatccc ttggggacct aagaaaggca aggaatacga cgacctgttc
     6601 aacgccatca gagtgtccga cagcaagccc ttcgacaaca aaagcctgat cctcgtgcag
     6661 aagtgggact acagcgccaa aaccaacaag gccaagcctc tgcctctgta cagagagtct
     6721 atcagccctc tgaccaagat cgagttcgag ataacaacaa ccactgatga ggccggcaga
     6781 ctgatcgagg aactgggaaa gcgggcccag gccttttata aggactacaa ggcctttttc
     6841 ctgtctgaat tccctgatga taagatccag gctaatctgc aataccccat ctacctgggc
     6901 gccggcagcg gcgcttggac aaagaccctg tttaagcagg ccgacggcat cctgcagcgg
     6961 agatactcca gaatgaaaac caagatggtc aagaagggcg tgctgaagct gacaaaggcc
     7021 cctctgaaaa cagtgaagat ccccagcggc aaccacagcc tggtgaagaa tcacgagagc
     7081 ttctacgaga tgggcaaagc caacttcatg atcaaggaaa tcgacaagtg acggcaataa
     7141 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca tgtgtgccgg ccggagaata
     7201 aaagtctaag tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     7261 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     7321 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     7381 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatctctaga
     7441 gccgccacca tggattacaa agacgatgac gataagatgg cgcctaagaa gaaacgcaaa
     7501 gtgcggggca tgaaaaagct cgtgttcacc tttaagcgga tcgaccaccc tgctcaggac
     7561 ctggccgtga aattccacgg cttcctgatg gaacagctgg atagcgacta cgtggactac
     7621 ctgcaccagc agcagaccaa cccctacgcc acaaaggtga tccagggcaa agagaacacc
     7681 cagtgggtcg tgcatctgct gacagacgac atcgaggaca aggtgttcat gaccctgctg
     7741 cagatcaagg aagtgtccct gaacgacctg cctaagttgt ctgtggaaaa ggtggaaatc
     7801 caggagctgg gcgctgataa gctgctcgag atcttcaaca gcgaggaaaa ccagacctac
     7861 ttcagcatca tcttcgagac acctacaggc tttaaaagcc agggcagcta cgtgatcttc
     7921 cccagcatgc ggctgatctt tcagagcctg atgcagaagt acggcagact ggtggaaaac
     7981 cagcctgaga tcgaggaaga taccctggac tacctgagcg agcacagcac catcaccaat
     8041 tacagactgg aaacaagcta cttcagagtg catagacaga gaatccccgc cttccggggc
     8101 aagctgacct tcaaggtgca gggagcccag acactgaagg cctacgtgaa gatgctgctg
     8161 accttcggcg agtacagcgg cctgggcatg aaaaccagcc tgggaatggg cggcatcaag
     8221 ctggaagaaa gaaaggactg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     8281 cgtttgttcg tgcgatagag ggatcccgca ttgaattatg tgatgcggtt ttggcagtac
     8341 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     8401 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     8461 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     8521 gctcgtttag tgaaccgtca gatctctaga gccgccacca tggtgagcaa gggcgaggag
     8581 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     8641 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     8701 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     8761 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     8821 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     8881 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     8941 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa aaccatgggc
     9001 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     9061 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     9121 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     9181 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     9241 accggcggca tggacgagct gtacaagtaa cggcaataaa aagacagaat aaaacgcacg
     9301 gtgttgggtc gtttgttcga gcagattgta ctgagagtgc accggttgga gggcctattt
     9361 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt
     9421 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
     9481 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
     9541 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgat
     9601 ataaacctaa ttacctcgag aggggacgga aacccgtctt cgatgaagcg attcagaaga
     9661 cttgatataa acctaattac ctcgagaggg gactttttta catgtgtcag aggttttcac
     9721 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     9781 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     9841 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9901 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9961 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10021 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10081 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10141 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10201 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10261 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10321 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10381 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10441 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10501 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10561 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    10621 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    10681 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10741 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10801 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10861 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10921 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10981 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11041 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11101 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11161 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11221 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11281 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11341 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11401 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11461 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11521 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    11581 ttttcttaag c
//
