LOCUS       Exported                7849 bp ds-DNA     circular UNA 21-DEC-2019
DEFINITION  natural linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 23, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          1..900
                     /organism="pcDNA3.1(+)"
                     /mol_type="other DNA"
                     /label=source:pcDNA3.1(+)
     source          1828..1833
                     /organism="pcDNA3.1(+)"
                     /mol_type="other DNA"
                     /label=source:pcDNA3.1(+)
                     /label=source:pcDNA3.1(+)(1)
     source          2467..2493
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     source          3304..3309
                     /organism="pcDNA3.1(+)"
                     /mol_type="other DNA"
                     /label=source:pcDNA3.1(+)
                     /label=source:pcDNA3.1(+)(2)
     promoter        236..852
                     /label=CMV_immearly_promoter
     misc_feature    315..602
                     /label=CAG_enhancer
     misc_feature    769..789
                     /label=CMV_fwd_primer
     promoter        863..881
                     /label=T7_promoter
     misc_feature    895..900
                     /label=NheI
                     /label=NheI(1)
     misc_feature    901..902
                     /label=NheI
     misc_feature    907..948
                     /label=wtATM
     misc_feature    949..954
                     /label=XhoI
     misc_feature    955..984
                     /label=linker
     misc_feature    985..990
                     /label=ECORV
     misc_feature    991..1017
                     /label=mycNLS
     CDS             1018..1734
                     /label=GFP
                     /note="/vntifkey=4"
     misc_feature    1018..1734
                     /label=FL GFP
     misc_feature    1735..1740
                     /label=BamHI
     misc_feature    1828..1833
                     /label=XbaI
     misc_feature    1834..1923
                     /label=3CC-Hex2
     misc_feature    1924..1929
                     /label=KpNI
     misc_feature    1930..1983
                     /label=T2A
     misc_feature    1984..1989
                     /label=hindIII
     misc_feature    1996..2424
                     /label=FHA1
     misc_feature    2425..2430
                     /label=xhoI
     misc_feature    2431..2460
                     /label=linker
                     /label=linker(1)
     misc_feature    2461..2466
                     /label=EcoRV
     CDS             2467..2493
                     /codon_start=1
                     /label=C-Myc NLS
                     /translation="PAAKRVKLD"
     CDS             2494..3210
                     /label=GFP
                     /label=GFP(1)
                     /note="/vntifkey=4"
     misc_feature    2494..3210
                     /label=FL GFP
                     /label=FL GFP(1)
     misc_feature    2692..2694
                     /label=Y66F
     misc_feature    3211..3216
                     /label=BamHI
                     /label=BamHI(1)
     misc_feature    3304..3309
                     /label=XbaI
                     /label=XbaI(1)
     misc_feature    3310..3408
                     /label=6_AntiTetramer2-WD
     misc_feature    3412..3417
                     /label=ECORI
     misc_feature    3427..3429
                     /label=Stop
     misc_feature    complement(3443..3460)
                     /label=BGH_rev_primer
     terminator      3446..3673
                     /label=bGH_PA_terminator
     rep_origin      3736..4042
                     /label=f1_origin
     misc_feature    complement(4156..4176)
                     /label=pBABE_3_primer
     misc_feature    complement(4162..4377)
                     /label=SV40_enhancer
     promoter        4174..4442
                     /label=SV40_promoter
     rep_origin      4341..4418
                     /label=SV40_origin
     misc_feature    4403..4422
                     /label=SV40pro_F_primer
     CDS             4557..5351
                     /codon_start=1
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR
                     /label=NeoR/KanR(1)
                     /note="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            4560..5348
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR
     terminator      5528..5647
                     /label=SV40_PA_terminator
     misc_feature    5616..5635
                     /label=EBV_rev_primer
     misc_feature    complement(5691..5709)
                     /label=M13_reverse_primer
     misc_feature    complement(5708..5730)
                     /label=M13_pUC_rev_primer
     promoter        complement(5744..5773)
                     /label=lac_promoter
     rep_origin      complement(6082..6698)
                     /direction=LEFT
                     /label=pBR322_origin
     CDS             complement(6853..7713)
                     /codon_start=1
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(6853..7713)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /label=Ampicillin(1)
     promoter        complement(7755..7783)
                     /label=AmpR_promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgt tggagacggt gagcacgcag gagttgtata gcataccgct cgagtccgga
      961 ctcagatctg gcagcggtgg aggcgatatc cccgccgcca agcgggtgaa gctggacatg
     1021 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1081 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1141 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1201 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1261 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1321 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1381 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1441 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1501 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1561 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1621 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1681 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggatcc
     1741 ggcagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg cagtgctggt
     1801 ggtagtgctg gtggtagtgc tggtggctct agaggagaaa tcgcaaagag tctgaaagaa
     1861 attgcaaaat ccttgaagga aatagcgtgg tcactgaaag aaattgccaa gtccctcaaa
     1921 gggggtaccg agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     1981 ccaaagcttg ccaccatgca taagttttct caagaacaga tcggcgaaaa cattgtgtgc
     2041 agggtcattt gtaccacggg tcaaattccc atccgagatt tgtcagctga tatttcacaa
     2101 gtgcttaagg aaaaacgatc cataaagaaa gtttggacat ttggtagaaa cccagcctgt
     2161 gactatcatt taggaaacat ttcaagactg tcaaataagc atttccaaat actactagga
     2221 gaagacggta accttttatt gaatgacatt tccactaatg ggacctggtt aaatgggcaa
     2281 aaagtcgaga agaacagcaa tcagttactg tctcaaggtg atgaaataac cgttggtgta
     2341 ggcgtggaat cagatatttt atctctggtc attttcataa acgacaaatt taagcagtgc
     2401 ctcgaacaga acaaagttga tcgcctcgag tccggactca gatctggcag cggtggaggc
     2461 gatatccccg ccgccaagcg ggtgaagctg gacatggtga gcaagggcga ggagctgttc
     2521 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2581 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2641 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac cttcggcgtg
     2701 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2761 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2821 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2881 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2941 aacgtctata tcatggccga caagcagaag aacggcatca aggccaactt caagatccgc
     3001 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     3061 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     3121 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3181 atcactctcg gcatggacga gctgtacaag ggatccggca gtgctggtgg tagtgctggt
     3241 ggtagtgctg gtggtagtgc tggtggcagt gctggtggta gtgctggtgg tagtgctggt
     3301 ggctctagaa ccttgagaga aatcgaggaa ttgttgagga aaatcattga agacagcgtg
     3361 cgcagtgtgg ccgagctgga ggacattgag aagtggctga agaaaatcta agaattcggg
     3421 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3481 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3541 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3601 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3661 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     3721 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3781 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3841 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3901 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3961 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4021 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4081 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4141 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4201 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4261 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4321 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4381 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4441 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4501 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     4561 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4621 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4681 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     4741 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4801 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4861 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4921 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4981 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5041 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5101 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5161 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5221 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5281 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5341 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5401 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5461 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5521 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5581 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5641 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5701 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5761 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5821 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5881 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5941 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6001 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6301 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6601 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     6661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
