LOCUS       pFB2G                 5655 bp    DNA     circular SYN 17-NOV-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(1491..2189)
                     /note="pLannotate"
                     /label="TagBFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      380..808
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2253..3380)
                     /note="pLannotate"
                     /label="TK"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(1025..1249)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(249..353)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(5043..5655,1..248))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4284..4872)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(3930..3960)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(3975..3996)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(3384..3402)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(3906..3922)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(839..993)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1266..1389)
                     /note="pLannotate"
                     /label="d2EYFP (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="14.7"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(3408..3479)
                     /note="pLannotate"
                     /label="PGK promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="14.4"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(4009..4101)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
       61 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      361 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      421 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      481 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      541 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      601 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      661 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      721 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      781 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt
      841 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      901 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      961 cccagtcacg acgttgtaaa acgacggcca gtggcggccg cccatcttcg ggaacatccg
     1021 gcatccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
     1081 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
     1141 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
     1201 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
     1261 cagcgctaca cattgatcct agcagaagca caggctgcag ggtgacggtc catcccgctc
     1321 tcctgggcac aagacatggg cagcgtgcca tcatcctgct cctccacctc cggcgggaag
     1381 ccatggctag atccagtgga tcccactttc cgttttttct tgggatcaac tttacgcttt
     1441 ttcttcggat ctacctttct cttctttttt ggatcagctc gagatctgag attaagcttg
     1501 tgccccagtt tgctagggag gtcgcagtat ctggccactg ccacctcgtg ctgctcgacg
     1561 taggtctcgt tgttggcctc cttgattctt tccagtctgt agtccacata gtagacgcca
     1621 ggcatcttga ggttcttagc gggtttcttg gatctatatg tggtcttgat gtttgcgatc
     1681 agatggctcc cgcccacgag cttcagggcc atgtcgtttc tgccttccag gccgccgtca
     1741 gcggggtaca gcgtttcggt gaaggcctcc cagccgagtg ttttcttctg catcacaggg
     1801 ccgttggatg tgaagttcac ccctctgatc ttgacgttgt agatgaggca gccgtcctgg
     1861 aggctggtgt cctgggtagc ggtcagcacg cccccgtctt cgtatgtggt gactctctcc
     1921 catgtgaagc cctcagggaa ggactgcttg aagaagtcgg ggatgccctg ggtgtggttg
     1981 atgaaggtct tgctgccgta gaggaagcta gtagccagga tgtcgaaggc gaaggggaga
     2041 gggccgccct cgaccacctt gattctcatg gtctgggtgc cctcgtaggg cttgccttcg
     2101 ccctcggatg tgcacttgaa gtgatggttg tccacggtgc cctccatgta cagcttcatg
     2161 tgcatgttct ccttaatcag ctcgctcatt gggccaggat tctcctcaac gtcaccgcat
     2221 gttagcagac ttcctctgcc ctctccactg ccgttagcct cccccatctc ccgggcaaac
     2281 gtgcgcgcca ggtcgcagat cgtcggtatg gagcctgggg tggtgacgtg ggtctggatc
     2341 atcccggagg taagttgcag cagggcgtcc cggcagccgg cgggcgattg gtcgtaatcc
     2401 aggataaaga cgtgcatggg acggaggcgt ttggccaaga cgtccaaggc ccaggcaaac
     2461 acgttgtaca ggtcgccgtt gggggccagc aactcggggg cccgaaacag ggtaaataac
     2521 gtgtccccga tatggggtcg tgggcccgcg ttgctctggg gctcggcacc ctggggcggc
     2581 acggccgtcc ccgaaagctg tccccaatcc tcccgccacg acccgccgcc ctgcagatac
     2641 cgcaccgtat tggcaagcag cccgtaaacg cggcgaatcg cggccagcat agccaggtca
     2701 agccgctcgc cggggcgctg gcgtttggcc aggcggtcga tgtgtctgtc ctccggaagg
     2761 gcccccaaca cgatgtttgt gccgggcaag gtcggcggga tgagggccac gaacgccagc
     2821 acggcctggg gggtcatgct gcccataagg tatcgcgcgg ccgggtagca caggagggcg
     2881 gcgatgggat ggcggtcgaa gatgagggtg agggccgggg gcggggcatg tgagctccca
     2941 gcctcccccc cgatatgagg agccagaacg gcgtcggtca cggcataagg catgcccatt
     3001 gttatctggg cgcttgtcat taccaccgcc gcgtccccgg ccgatatctc accctggtca
     3061 aggcggtgtt gtgtggtgta gatgttcgcg attgtctcgg aagcccccag cacccgccag
     3121 taagtcatcg gctcgggtac gtagacgata tcgtcgcgcg aacccagggc caccagcagt
     3181 tgcgtggtgg tggttttccc catcccgtgg ggaccgtcta tataaacccg cagtagcgtg
     3241 ggcattttct gctccgggcg gacttccgtg gcttcttgct gccggcgagg gcgcaacgcc
     3301 gtacgtcggt tgctatggcc gcgagaacgc gcagcctggt cgaacgcaga cgcgtgctga
     3361 tggccggggt acgaagccat ccccctatag tgagtcgtat tactagactt ttgaagcgtg
     3421 cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctt
     3481 cccagcctct gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg
     3541 cctacccgct tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct
     3601 acttccattt gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact
     3661 aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta
     3721 ccggtggatg tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc
     3781 ggggctgcta aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag
     3841 aattccctcc gcgaattaat agctcctggg cgcgcctcat ggtcatagct gtttcctgtg
     3901 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3961 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4021 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4081 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4141 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4201 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4261 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4321 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4381 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4441 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4501 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4621 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4681 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4741 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4801 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4861 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4921 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4981 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5041 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5101 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5161 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5221 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5281 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5341 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5401 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5461 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5521 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5581 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5641 tgctcttgcc cggcg
//
