LOCUS       pRC0431-pLego-G2-MCS-sEF1-Blasticidin        7319 bp ds-DNA     circular SYN 25-DEC-2019
DEFINITION  synthetic circular DNA.
ACCESSION
VERSION     .
KEYWORDS    LeGO-G2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Weber K, Bartsch U, Stocking C and Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO)
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     misc_feature    231..236
                     /Recognition_pattern="A^CGCGT"
                     /annotation_group="ACGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     enhancer        238..617
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     misc_feature    252..257
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    486..491
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    591..596
                     /Recognition_pattern="TAC^GTA"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     misc_feature    591..596
                     /Recognition_pattern="YAC^GTR"
                     /annotation_group="YACGTR"
                     /label="BsaAI, BstBAI, Ppu21I"
                     /note="Geneious type: restriction site"
     promoter        619..817
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     772..792
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     LTR             835..1015
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    1018..1023
                     /Recognition_pattern="GGC^GCC"
                     /annotation_group="GGCGCC"
                     /label="DinI, EgeI, EheI, SfoI"
                     /note="Geneious type: restriction site"
     misc_feature    1018..1023
                     /Recognition_pattern="GGCGC^C"
                     /annotation_group="GGCGCC"
                     /label="PluTI"
                     /note="Geneious type: restriction site"
     misc_feature    1018..1023
                     /Recognition_pattern="GG^CGCC"
                     /annotation_group="GGCGCC"
                     /label="NarI, Mly113I"
                     /note="Geneious type: restriction site"
     misc_feature    1018..1023
                     /Recognition_pattern="G^GCGCC"
                     /annotation_group="GGCGCC"
                     /label="KasI, SspDI"
                     /note="Geneious type: restriction site"
     misc_feature    1056..1066
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8)"
                     /annotation_group="GAGNNNNNCTC"
                     /label="BplI"
                     /note="Geneious type: restriction site"
     misc_feature    1062..1187
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /label="HIV-1 Psi"
     misc_feature    complement(1488..1494)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    1526..1533
                     /Recognition_pattern="GGCCGG^CC"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    1686..1919
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /label="RRE"
     misc_feature    1956..1965
                     /Recognition_pattern="CACNN^NNGTG"
                     /annotation_group="CACNNNNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    1971..1976
                     /Recognition_pattern="GAATGC(1/-1)"
                     /annotation_group="GAATGC"
                     /label="BsmI, Mva1269I, PctI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2209..2219)
                     /Recognition_pattern="(9/12)ACNNNNNCTCC(10/7)"
                     /annotation_group="ACNNNNNCTCC"
                     /label="BsaXI"
                     /note="Geneious type: restriction site"
     misc_feature    2316..2322
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    2446..2563
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     misc_feature    2608..2613
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2608..2613
                     /Recognition_pattern="G^GGCCC"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     primer_bind     2622..2638
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="KS primer"
     PCR_primer      2632..2651
                     /created_by="charir2"
                     /label="cPCR-F"
     protein_bind    2680..2713
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /label="loxP"
     source          <2734..>3655
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     PCR_primer      2734..2753
                     /created_by="charir2"
                     /label="F"
     promoter        2734..2980
                     /created_by="charir2"
                     /label="sEF1"
     misc_feature    2813..2818
                     /Recognition_pattern="A^CCGGT"
                     /annotation_group="ACCGGT"
                     /label="AgeI, AsiGI, BshTI, CspAI, PinAI"
                     /note="Geneious type: restriction site"
     misc_feature    2969..2976
                     /Recognition_pattern="VC^TCGAGB"
                     /annotation_group="VCTCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    2970..2975
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI, PaeR7I, Sfr274I, SlaI"
                     /note="Geneious type: restriction site"
     gene            2981..3379
                     /created_by="charir2"
                     /label="Blasticidin"
     misc_feature    2982..2987
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI, MlsI, MluNI, Mox20I, MscI, Msp20I"
                     /note="Geneious type: restriction site"
     misc_feature    3275..3284
                     /Recognition_pattern="GATNN^NNATC"
                     /annotation_group="GATNNNNATC"
                     /label="BsaBI, Bse8I, BseJI"
                     /note="Geneious type: restriction site"
     misc_feature    3391..3396
                     /Recognition_pattern="T^GATCA"
                     /annotation_group="TGATCA"
                     /label="BclI, FbaI, Ksp22I"
                     /note="Geneious type: restriction site"
     polyA_signal    3404..3611
                     /created_by="charir2"
                     /label="BGHPolyA"
     misc_feature    3600..3605
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI, PaeI"
                     /note="Geneious type: restriction site"
     PCR_primer      complement(3636..3655)
                     /created_by="charir2"
                     /label="End"
     PCR_primer      complement(3658..3678)
                     /created_by="charir2"
                     /label="cpCR-R"
     promoter        3660..4066
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
                     /label="SFFV promoter"
     misc_feature    3790..3795
                     /Recognition_pattern="C^CCGGG"
                     /annotation_group="CCCGGG"
                     /label="Cfr9I, TspMI, XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    3790..3795
                     /Recognition_pattern="CCC^GGG"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    3809..3815
                     /Recognition_pattern="G^GTNACC"
                     /annotation_group="GGTNACC"
                     /label="BstEII, BstPI, Eco91I, EcoO65I, PspEI"
                     /note="Geneious type: restriction site"
     misc_feature    4032..4039
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     misc_feature    4052..4060
                     /Recognition_pattern="GACN^NNGTC"
                     /annotation_group="GACNNNGTC"
                     /label="Tth111I, PflFI, PsyI"
                     /note="Geneious type: restriction site"
     regulatory      4072..>4077
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                      of translation (Kozak, 1987)"
     misc_feature    4078..4099
                     /Original_Bases="ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCC
                     CATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAG
                     GGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA
                     AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTT
                     CAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAA
                     GGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCG
                     CCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGA
                     CTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCAC
                     AACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCC
                     GCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCC
                     CATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCC
                     CTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCG
                     CCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA"
                     /label="ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
                     CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGC
                     GATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCG
                     TGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTA
                     CCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTC
                     CAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGA
                     AGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGA
                     GGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTAT
                     ATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACA
                     TCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGA
                     CGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAA
                     GACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA
                     TCACTCTCGGCATGGACGAGCTGTACAAGTAA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4078..4085
                     /Recognition_pattern="CCTGCA^GG"
                     /annotation_group="CCTGCAGG"
                     /label="Sse8387I, SbfI, SdaI"
                     /note="Geneious type: restriction site"
     misc_feature    4086..4091
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    4092..4099
                     /Recognition_pattern="GTTT^AAAC"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI, MssI"
                     /note="Geneious type: restriction site"
     misc_feature    4100..4105
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     protein_bind    4119..4152
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /label="loxP"
     misc_feature    4153..4158
                     /Recognition_pattern="A^CATGT"
                     /annotation_group="ACATGT"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     misc_feature    4208..4796
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /label="WPRE"
     primer_bind     complement(4261..4281)
                     /note="WPRE, reverse primer"
                     /label="WPRE-R"
     misc_feature    4443..4453
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI, AccB7I, Van91I"
                     /note="Geneious type: restriction site"
     misc_feature    4481..4487
                     /Recognition_pattern="T^CCNGGA"
                     /annotation_group="TCCNGGA"
                     /label="PfoI"
                     /note="Geneious type: restriction site"
     CDS             complement(4679..4690)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /label="Factor Xa site"
     misc_feature    4708..4713
                     /Recognition_pattern="CCGC^GG"
                     /annotation_group="CCGCGG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(4799..4815)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="KS primer"
     misc_feature    4929..4935
                     /Recognition_pattern="CC^TNAGG"
                     /annotation_group="CCTNAGG"
                     /label="AxyI, Bse21I, Bsu36I, Eco81I"
                     /note="Geneious type: restriction site"
     misc_feature    4934..4939
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    4934..4939
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Acc65I, Asp718I"
                     /note="Geneious type: restriction site"
     misc_feature    5312..5317
                     /Recognition_pattern="T^CCGGA"
                     /annotation_group="TCCGGA"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     LTR             5325..5505
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     rep_origin      complement(5567..6155)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(5647..5666)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(6326..7186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     misc_feature    6394..6404
                     /Recognition_pattern="GACNNN^NNGTC"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    6619..6624
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    6877..6882
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    6915..6926
                     /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)"
                     /annotation_group="CGANNNNNNTGC"
                     /label="BcgI"
                     /note="Geneious type: restriction site"
     primer_bind     6949..6968
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(7187..7291)
                     /gene="bla"
                     /label="AmpR promoter"
     misc_feature    7201..7206
                     /Recognition_pattern="AAT^ATT"
                     /annotation_group="AATATT"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     misc_feature    7319..7
                     /Recognition_pattern="CG^TCGACG"
                     /annotation_group="CGTCGACG"
                     /label="SgrDI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag tcgaggtcga cggtatcgat
     2641 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
     2701 tatacgaagt tatattaagg gttccaagct taaaggctcc ggtgcccgtc agtgggcaga
     2761 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     2821 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     2881 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     2941 caacgggttt gccgccagaa cacaggatcc tcgagccacc atggccaagc ctttgtctca
     3001 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     3061 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     3121 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     3181 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     3241 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     3301 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     3361 ttatgtgtgg gagggctaac tagaggtcgc tgatcagcct cgactgtgcc ttctagttgc
     3421 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3481 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3541 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga gaatagcagg
     3601 catgctgggg aaaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctgcag
     3661 taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa
     3721 gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt
     3781 cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc
     3841 aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat
     3901 gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca
     3961 gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca
     4021 acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccggatcc cgccacccct
     4081 gcaggtctag agtttaaacg aattcgtcga gggacctaat aacttcgtat agcatacatt
     4141 atacgaagtt atacatgttt aagggttccg gttccactag gtacaattcg atatcaagct
     4201 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4261 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4321 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4381 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4441 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4501 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4561 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     4621 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4681 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4741 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     4801 ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     4861 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     4921 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4981 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     5041 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     5101 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     5161 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     5221 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     5281 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     5341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag
     5521 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6001 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
