LOCUS       pBAD322-MycaIDCa        9881 bp DNA     circular SYN 06-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Cornell University
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2022 from SnapGene Viewer 6.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9881
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             1320..4169
                     /codon_start=1
                     /transl_table=11
                     /gene="cas10d"
                     /locus_tag="KME13_07080"
                     /product="type I-D CRISPR-associated protein Cas10d/Csc3"
                     /label=cas10d
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:HMM"
                     /protein_id="MBW4418978.1"
                     /translation="MKPLLQTLLLQTLPSDIDPVLLAYVETVLPAVEQEFGLIPVSNDV
                     SQTEQSLLVHILNSLLTAWNLFPFLSSKLALSDNEKRLLCLSLIFQNDNSKCQSRENNS
                     PPTLGVNETLKQYRAFGDKLNLAAFWTEWREYLTDIVYLTQSTQPHALSIKKTALKIPA
                     NRLELPLRPLLLFGAIAAQLTDPADIVIKPEGNVLKECLKRLGVQHQLTYHRVRACTGL
                     LTEGIHNAIMRFVQPLDWQSILFFAQGIVYLVPSDSKLLDCSKLQTFIWEQISEQLAIS
                     MLRGKVGFKRDGKGMKAAPQTLELFSPAQFIRNLPTVINACVKNETEPATPKRLANLDL
                     DPTDREFLACGADLRSDRIAEFILFLQKKFFGTNPEFIDRVLDQLGIQSAITPEQTQIF
                     SSGVNRGWYHAAAYYVASNPHLDSDDVTIKLQEIANQLVDWAEEKELFPPQISPTREVF
                     NQYLAQYLEFPRWNNSAPSFQHELATYVAAKTKAAKQPICSLSSSEFDSEDQLDSVVLF
                     KPQQYSNKNPLGGWQLKRGISKLWSLEMLLRQSFWSVSAGKLEERQPVFLYFFPSLVHS
                     PQVAKAIRILVNQLKRVNLWKVCRFWQVHGMNANTLRFYSWLKSDPRPDDNGKRDLPFL
                     AITYTTTAGKTTTQAWIEPAFLVLALSLVLGIKVVATASPVPLYNRSSEFPETVKIDGP
                     ASFWSLLGLPTSIRLEEKIQGRVKHLNDWLDRLLIAYSIHLDCQANPPDSRWQLFPGTV
                     QAITTDVSAIFSLAHSHFQSLSDDRVQRYWHFAQVWAAGDSNMEQQLELTQRLVQEYRA
                     FYQVHVAKATHAIVLPLLKVLEEILSTPSDLPIETLILQTSGRLHDALKRQEPYKRPLI
                     MDQSLSIEIRVNNEIQAIQRFVTTCINDLFLAQYKGDRALLQEHRNRIKSGAEFAYRLL
                     AMQEPKPKTKKQEIEGAQI"
     gene            1320..4169
                     /gene="cas10d"
                     /locus_tag="KME13_07080"
                     /label=cas10d
     variation       1590..1595
                     /label=HD->QN nuclease dead
                     /note="cd09696"
     CDS             3705..4169
                     /codon_start=1
                     /transl_table=11
                     /product="according to paper: 
                     https://www.sciencedirect.com/science/article/pii/S10972765
                     20307814#fig3"
                     /label=cas11d?
                     /translation="MEQQLELTQRLVQEYRAFYQVHVAKATHAIVLPLLKVLEEILSTP
                     SDLPIETLILQTSGRLHDALKRQEPYKRPLIMDQSLSIEIRVNNEIQAIQRFVTTCIND
                     LFLAQYKGDRALLQEHRNRIKSGAEFAYRLLAMQEPKPKTKKQEIEGAQI"
     CDS             4166..5203
                     /codon_start=1
                     /transl_table=11
                     /gene="cas7d"
                     /locus_tag="KME13_07085"
                     /product="type I-D CRISPR-associated protein Cas7/Csc2"
                     /label=cas7d
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="MBW4418979.1"
                     /translation="MSVLEELKPQFHSAFPRLASGKYVHVFLVRHSQSFPVFQTDGVLN
                     TARTQAGLDSKDDKSRQSINRLVMFKRKQVTPERLVGRQLLRSLGLTSAVEGEVNFCEY
                     NSQKSCKKCPDCIIYGFGVGDKGAERSKVYSDSAFSLSPYETSHRSFTFNAPSENGTMS
                     EKGELRSSINDLDHVLPEVMFPSVETLRDPTFEGFIYVLGNLIRTRRYGGQESRTGTMM
                     NHIVGIAFCDGEVFSNLYFTQALYDALNAKGDICVTIEDFRHEAARVAKNCLKQEPVRS
                     ITIFGAELDALLEEVTGIYQDEVKQRLIMTTLYQQTQIYSESYGAIFPEKKKEEKKKAQ
                     QGSSI"
     gene            4172..5203
                     /gene="cas7d"
                     /locus_tag="KME13_07085"
                     /label=cas7d
     CDS             5215..6001
                     /codon_start=1
                     /transl_table=11
                     /gene="cas5d"
                     /locus_tag="KME13_07090"
                     /product="type I-D CRISPR-associated protein Cas5/Csc1"
                     /label=cas5d
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="MBW4418980.1"
                     /translation="MMVFYFCHLTLHDNVFFASREMGTLYETEKYLHNWALSYALFESA
                     YIPRPYRLEGAVAQRPTYLEIDSEQNLLHLNQQGIYVFPALPLQWTYQVNTFNVSQAFY
                     HQKSRRLGSGGHILNYPISYGRAKELAVGSKYRTFIIAPAKTRIPRWIRLGKWSAKIRV
                     EIIPIPKSGIQRAAGEYVSEHPLNPVDLSKTTRLLLYNRIVMPPSSLVSQAKLLGDYWE
                     ISKPAQWAKLSEWSSLENLPETICLPRGVAYGANTLVTTP"
     gene            5215..6001
                     /gene="cas5d"
                     /locus_tag="KME13_07090"
                     /label=cas5d
     CDS             5972..6001
                     /codon_start=1
                     /transl_table=11
                     /gene="cas6"
                     /locus_tag="KME13_07095"
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /label=cas6
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="MBW4418981.1"
                     /translation="MELTLSLPHL"
     gene            5972..6001
                     /gene="cas6"
                     /locus_tag="KME13_07095"
                     /label=cas6
     terminator      6021..6048
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        6055..6089
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        6101..6137
                     /label=Hammerhead ribozyme
     misc_feature    6138..6145
                     /label=5' handle
     misc_feature    6146..6180
                     /label=lacZsp5
     misc_RNA        6181..6248
                     /label=HDV ribozyme
     primer_bind     6249..6251
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      6495..6581
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6673..6700
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6719..6810
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6811..7671
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7713..8168
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      8279..8867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9053..9193
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    9108..9179
                     /label=missing from pBAD24
     CDS             complement(9295..9486)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    9336..9881
                     /label=not present in pBAD24
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
     1321 tgaagccctt actccagact cttctacttc aaactctacc gtctgacatt gatccagtat
     1381 tgctggctta tgttgagact gtattacctg ctgtagaaca ggagttcggt ctgattccag
     1441 tttctaacga tgttagtcaa actgagcaaa gcttgctggt tcacattctc aatagtttgc
     1501 tgactgcctg gaacttgttc ccgttcctat cctcaaaatt ggcgctatca gacaacgaga
     1561 aacgattgct ttgcttgagt cttatctttc aaaatgacaa cagtaagtgc cagagccgag
     1621 agaataattc gcctcctacg cttggtgtta atgaaaccct aaaacagtac cgagcatttg
     1681 gagacaaact aaatctggcg gcgttctgga ctgagtggcg tgagtatttg acagatatag
     1741 tttatttaac ccaaagcacc caaccgcatg ctttgtcaat caagaagact gctctcaaaa
     1801 ttcctgctaa tcgcctagag ttgcctctac gtccattgct tctgtttggg gcgatcgctg
     1861 cccagcttac tgatcccgct gatattgtca tcaaaccgga aggcaatgtc ttaaaagaat
     1921 gcttgaaacg tctgggtgtt cagcatcaac tcacttatca tcgagtacgc gcctgcacag
     1981 gactcttaac tgagggcatc cacaatgcca ttatgcgttt tgtacaacct ctcgactggc
     2041 aatccattct gttctttgcc caagggatcg tttatttggt tccctctgac agcaaactgc
     2101 ttgattgctc aaaactacaa acctttatct gggagcagat tagtgagcag ttagcaatca
     2161 gtatgcttcg aggaaaagtt ggtttcaagc gagatggcaa agggatgaaa gctgcgcctc
     2221 aaacgctgga gctattctca ccagctcaat tcattcggaa tctacccact gtcattaatg
     2281 cctgtgtcaa gaatgagacg gaacctgcaa cacctaaacg actggcaaat cttgaccttg
     2341 atccaacaga tcgagaattt ttagcttgtg gggcggatct aagaagcgat cgcattgcag
     2401 aatttatcct tttcttgcag aagaagtttt ttgggactaa tcctgaattt atcgatcgag
     2461 ttctcgacca actgggcatt caatctgcca tcactccaga acagactcaa atcttctcaa
     2521 gcggggtaaa taggggatgg tatcacgcag ccgcttatta cgtcgccagt aaccctcact
     2581 tagactcaga tgatgtaacg atcaagcttc aggagatcgc caatcaacta gtggactggg
     2641 ctgaagagaa agaacttttt ccacctcaaa tcagtccgac tcgtgaggtt ttcaatcaat
     2701 acttagctca gtacttggaa ttcccaaggt ggaataattc agcgccatcc tttcaacatg
     2761 aattggcaac gtatgtagcg gcaaaaacaa aagccgctaa acaaccaatt tgctccctca
     2821 gttccagtga atttgactca gaagaccagc tagattctgt tgtgctattc aagcctcagc
     2881 agtatagcaa caagaatccc ctcggagggt ggcaactgaa gcgaggaatt tctaaactct
     2941 ggtcattaga gatgcttctg cggcagtctt tctggtcagt ttccgcaggc aagctggaag
     3001 agcgacaacc tgtctttctg tacttttttc ccagtttggt tcattcccct caggtcgcaa
     3061 aagcgattcg tatcctagtc aatcaactta aacgagtcaa tttgtggaaa gtttgtcgat
     3121 tctggcaggt acacgggatg aatgctaata ccctgcgctt ctactcttgg ctgaagtctg
     3181 atcccagacc agacgataat ggtaaacgtg atctcccttt cctagcaatc acttacacca
     3241 ccacagctgg taaaacgacg acacaggctt ggattgaacc tgcttttttg gtattggcgc
     3301 tctcacttgt gctgggcatt aaagtagtcg ctacggcaag tccagtacca ctttataaca
     3361 ggagtagcga gttccccgaa acagtgaaga tagacggacc tgcgagcttc tggagcctgt
     3421 tgggtttgcc aaccagcatt cggctggaag agaagatcca gggacgggtc aaacacctga
     3481 atgattggct cgatcgccta ctaattgctt acagtattca ccttgactgt caagcgaatc
     3541 cccctgactc tcgttggcaa ttgtttcccg gtacagtgca ggctataaca actgatgtct
     3601 cagcgatttt tagccttgct cattcgcatt ttcaaagtct ttcggatgac agagttcaac
     3661 gatactggca ctttgctcaa gtatgggcag caggagattc aaacatggaa cagcaattgg
     3721 agctaactca gcgactggtc caggaatatc gcgcgttcta ccaagttcat gtagcgaaag
     3781 caacccacgc gatcgtgctc ccgctgctaa aggttttaga ggagattctt tcaactccta
     3841 gtgatctgcc aattgaaact ctgatattac agacatcggg acggttacat gacgctttga
     3901 agcgacagga accctacaaa cgccccctga ttatggatca gtctttatca attgagattc
     3961 gtgtcaataa cgagattcag gcaattcaac gatttgtcac cacttgcatc aatgatttat
     4021 ttttagctca gtacaaaggc gatcgcgccc tactgcaaga acaccgtaac cgaattaaat
     4081 cgggtgcaga gtttgcgtat cgtttactag ctatgcagga gccaaaacca aaaacgaaaa
     4141 agcaagaaat tgaaggagca cagatatgag tgtgttagaa gaattgaaac ctcaattcca
     4201 ttcggctttt ccccgtctgg catccgggaa gtatgttcat gtcttcctgg tacgtcacag
     4261 tcaatccttt cccgtgtttc aaacagatgg tgtcctcaat acggctcgaa ctcaagctgg
     4321 cttggattcc aaagatgata aatctaggca atcgatcaac cgattggtca tgttcaagcg
     4381 caagcaagta acacccgaac gactggttgg acgacaactg ctacgatcgc taggactaac
     4441 cagtgctgta gaaggcgaag tcaacttttg cgaatacaac agtcagaaat cgtgcaagaa
     4501 atgcccagac tgtattatct acggattcgg ggttggagac aaaggtgcag aacgctcgaa
     4561 agtctactca gactcagcgt tctcgctcag tccttatgaa acttcccacc gcagtttcac
     4621 cttcaatgcg ccgtcagaaa atggcacgat gagtgaaaag ggagaactgc gaagttcgat
     4681 caacgatcta gaccatgtcc tgccggaagt gatgtttcct agtgttgaaa cgctgcgtga
     4741 tcccacattt gaggggttta tttatgtgct gggcaacctg attcgcacgc gccgctatgg
     4801 aggtcaagaa tcccgaaccg ggacgatgat gaatcacatt gttggtattg ccttttgcga
     4861 tggtgaagtt ttcagcaact tgtatttcac acaagcgcta tatgatgcct tgaatgcaaa
     4921 gggggatatt tgcgtgacga tcgaggactt tcgacatgag gcagcaaggg tggcaaaaaa
     4981 ctgtctgaaa caggagccag ttcgcagcat cacaattttt ggggctgaac tcgatgcctt
     5041 gctcgaagaa gtgacgggca tctatcagga tgaagttaaa cagcgcctta ttatgaccac
     5101 gctttaccaa cagacccaga tctacagtga aagctacgga gcaatttttc ctgagaagaa
     5161 gaaagaggaa aagaaaaaag cacagcaagg ctcttcgatc tgagggatgg tggaataatg
     5221 gttttctatt tttgccattt aaccttgcat gacaatgttt tctttgcctc acgggagatg
     5281 ggaactctct atgagacaga aaaatacctg cacaattggg cacttagcta tgcgctattt
     5341 gaaagcgcct acattcctag accttatcga ttggaagggg cagtcgccca gcgtcctacg
     5401 tatctagaga ttgatagtga gcaaaacctg ttacatctga atcagcaagg aatttatgtt
     5461 tttcccgccc tgccgcttca gtggacatat caggtcaata cattcaatgt gtctcaagcc
     5521 ttctaccatc aaaaatcaag acggttgggc tcgggaggac atattctcaa ttacccaatc
     5581 agctatggac gggcaaagga actcgcagtt ggcagcaagt acagaacctt catcattgct
     5641 cctgccaaaa cgagaatccc ccgctggata agactaggca aatggtcagc gaaaattcga
     5701 gtcgagatca tccccattcc taaaagtgga attcaaaggg cagcaggcga gtatgtttct
     5761 gagcatcccc tcaatcctgt agatctctct aaaacgaccc gattattgct gtataaccgg
     5821 attgtcatgc caccttctag tctggtgagc caggcaaaat tgttgggtga ctattgggag
     5881 atctcaaaac cagctcagtg ggctaaattg tctgagtggt caagcttaga gaatttgcct
     5941 gaaacgattt gtttgccaag aggagttgct tatggagcta acactctcgt taccacacct
     6001 taactaaaaa aaatcaaact ggctcacctt cgggtgggcc tttttgcgtt tatattgaca
     6061 gctagctcag tcctaggtat aatgctagct acttttcagt ctgatgagtc cgtgaggacg
     6121 aaacgagtaa gctcgtcact gaaaggcact acgcgtactg tgagccagag ttgcccggcg
     6181 ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg
     6241 aatgggacgt acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt
     6301 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     6361 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     6421 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     6481 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     6541 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     6601 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
     6661 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
     6721 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6781 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6841 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6901 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6961 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7021 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     7081 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7141 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7201 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7261 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7321 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7381 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7441 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7501 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7561 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7621 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7681 caagtttact catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc
     7741 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7801 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7861 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7921 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7981 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact
     8041 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8101 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     8161 tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8401 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8941 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9001 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     9061 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     9121 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     9181 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     9241 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     9301 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     9361 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     9421 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     9481 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     9541 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     9601 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     9661 tcaatgccag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
     9721 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
     9781 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
     9841 agcaatttaa ctgtgataaa ctaccgcatt aaagctagct t
//
