LOCUS       pRC0434-pLego-G2-MCS-sEF1-Neomycin        7715 bp ds-DNA     circular SYN 19-JAN-2021
DEFINITION  synthetic circular DNA.
ACCESSION
VERSION     .
KEYWORDS    LeGO-G2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Weber K, Bartsch U, Stocking C and Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO)
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     misc_feature    231..236
                     /Recognition_pattern="A^CGCGT"
                     /Methylation="CpG:blocked, EcoBI:variable, EcoKI:variable"
                     /annotation_group="ACGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     enhancer        238..617
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     misc_feature    252..257
                     /Recognition_pattern="A^CTAGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    486..491
                     /Recognition_pattern="CA^TATG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    591..596
                     /Recognition_pattern="TAC^GTA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     promoter        619..817
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     772..792
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     LTR             835..1015
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    1056..1066
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8)"
                     /annotation_group="GAGNNNNNCTC"
                     /label="BplI"
                     /note="Geneious type: restriction site"
     misc_feature    1062..1187
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /label="HIV-1 Psi"
     misc_feature    1526..1533
                     /Recognition_pattern="GGCCGG^CC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    1686..1919
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /label="RRE"
     misc_feature    1956..1965
                     /Recognition_pattern="CACNN^NNGTG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CACNNNNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    1971..1976
                     /Recognition_pattern="GAATGC(1/-1)"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GAATGC"
                     /label="BsmI, Mva1269I, PctI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2209..2219)
                     /Recognition_pattern="(9/12)ACNNNNNCTCC(10/7)"
                     /Methylation="Dcm:cut, CpG:cut"
                     /annotation_group="ACNNNNNCTCC"
                     /label="BsaXI"
                     /note="Geneious type: restriction site"
     misc_feature    2316..2322
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    2446..2563
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     misc_feature    2608..2613
                     /Recognition_pattern="GGGCC^C"
                     /Methylation="Dcm:some blocked, CpG:some blocked"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2608..2613
                     /Recognition_pattern="G^GGCCC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     primer_bind     2622..2638
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="KS primer"
     PCR_primer      2632..2651
                     /created_by="charir2"
                     /label="cPCR-F"
     protein_bind    2680..2713
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /label="loxP"
     source          <2734..>4051
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     PCR_primer      2734..2753
                     /created_by="charir2"
                     /label="F"
     promoter        2734..2980
                     /created_by="charir2"
                     /label="sEF1"
     misc_feature    2813..2818
                     /Recognition_pattern="A^CCGGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACCGGT"
                     /label="AgeI, AsiGI, BshTI, CspAI, PinAI"
                     /note="Geneious type: restriction site"
     misc_feature    2969..2976
                     /Recognition_pattern="VC^TCGAGB"
                     /Methylation="CpG:some impaired, EcoBI:cut, EcoKI:cut"
                     /annotation_group="VCTCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    2970..2975
                     /Recognition_pattern="C^TCGAG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CTCGAG"
                     /label="XhoI, PaeR7I, Sfr274I, SlaI"
                     /note="Geneious type: restriction site"
     gene            2981..3775
                     /created_by="charir2"
                     /label="Neomycin"
     source          <2981..>3775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2985..3774
                     /Original_Bases="CCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGAC
                     GTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCC
                     ACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCT
                     CACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTG
                     GCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCC
                     CGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCT
                     CCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCG
                     CCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGG
                     CCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTT
                     CTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGC
                     ACCTGGTGCATGACCCGCAAGCCCGGTGCCTG"
                     /label="CCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGG
                     GCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCG
                     ATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGT
                     CGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGG
                     ACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGG
                     CCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCC
                     GCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCAC
                     CAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCG
                     CCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCG
                     GCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGC
                     ATGACCCGCAAGCCCGGTGCCTG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3189..3194
                     /Recognition_pattern="TGG^CCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGGCCA"
                     /label="BalI, MlsI, MluNI, Mox20I, MscI, Msp20I"
                     /note="Geneious type: restriction site"
     misc_feature    3624..3630
                     /Recognition_pattern="CG^GWCCG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGGWCCG"
                     /label="RsrII, CpoI, CspI, Rsr2I"
                     /note="Geneious type: restriction site"
     misc_feature    3787..3792
                     /Recognition_pattern="T^GATCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGATCA"
                     /label="BclI, FbaI, Ksp22I"
                     /note="Geneious type: restriction site"
     polyA_signal    3800..4007
                     /created_by="charir2"
                     /label="BGHPolyA"
     PCR_primer      complement(4032..4051)
                     /created_by="charir2"
                     /label="End"
     PCR_primer      complement(4054..4074)
                     /created_by="charir2"
                     /label="cpCR-R"
     promoter        4056..4462
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
                     /label="SFFV promoter"
     misc_feature    4186..4191
                     /Recognition_pattern="C^CCGGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCCGGG"
                     /label="Cfr9I, TspMI, XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    4186..4191
                     /Recognition_pattern="CCC^GGG"
                     /Methylation="CpG:blocked"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    4205..4211
                     /Recognition_pattern="G^GTNACC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGTNACC"
                     /label="BstEII, BstPI, Eco91I, EcoO65I, PspEI"
                     /note="Geneious type: restriction site"
     misc_feature    4428..4435
                     /Recognition_pattern="GG^CGCGCC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     regulatory      4468..>4473
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                      of translation (Kozak, 1987)"
     misc_feature    4474..4495
                     /Original_Bases="ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCC
                     CATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAG
                     GGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA
                     AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTT
                     CAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAA
                     GGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCG
                     CCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGA
                     CTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCAC
                     AACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCC
                     GCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCC
                     CATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCC
                     CTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCG
                     CCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA"
                     /label="ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
                     CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGC
                     GATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCG
                     TGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTA
                     CCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTC
                     CAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGA
                     AGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGA
                     GGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTAT
                     ATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACA
                     TCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGA
                     CGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAA
                     GACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA
                     TCACTCTCGGCATGGACGAGCTGTACAAGTAA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4474..4481
                     /Recognition_pattern="CCTGCA^GG"
                     /annotation_group="CCTGCAGG"
                     /label="Sse8387I, SbfI, SdaI"
                     /note="Geneious type: restriction site"
     misc_feature    4482..4487
                     /Recognition_pattern="T^CTAGA"
                     /Methylation="Dam:blocked"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    4488..4495
                     /Recognition_pattern="GTTT^AAAC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI, MssI"
                     /note="Geneious type: restriction site"
     misc_feature    4496..4501
                     /Recognition_pattern="G^AATTC"
                     /Methylation="CpG:variable, EcoBI:variable"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     protein_bind    4515..4548
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /label="loxP"
     misc_feature    4549..4554
                     /Recognition_pattern="A^CATGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACATGT"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     misc_feature    4604..5192
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /label="WPRE"
     primer_bind     complement(4657..4677)
                     /note="WPRE, reverse primer"
                     /label="WPRE-R"
     misc_feature    4839..4849
                     /Recognition_pattern="CCANNNN^NTGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI, AccB7I, Van91I"
                     /note="Geneious type: restriction site"
     misc_feature    4877..4883
                     /Recognition_pattern="T^CCNGGA"
                     /annotation_group="TCCNGGA"
                     /label="PfoI"
                     /note="Geneious type: restriction site"
     CDS             complement(5075..5086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /label="Factor Xa site"
     misc_feature    5104..5109
                     /Recognition_pattern="CCGC^GG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCGCGG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(5195..5211)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="KS primer"
     misc_feature    5325..5331
                     /Recognition_pattern="CC^TNAGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCTNAGG"
                     /label="AxyI, Bse21I, Bsu36I, Eco81I"
                     /note="Geneious type: restriction site"
     misc_feature    5330..5335
                     /Recognition_pattern="GGTAC^C"
                     /Methylation="Dcm:cut, CpG:cut"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    5330..5335
                     /Recognition_pattern="G^GTACC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGTACC"
                     /label="Acc65I, Asp718I"
                     /note="Geneious type: restriction site"
     misc_feature    5708..5713
                     /Recognition_pattern="T^CCGGA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TCCGGA"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     LTR             5721..5901
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     rep_origin      complement(5963..6551)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(6043..6062)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(6722..7582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     misc_feature    6790..6800
                     /Recognition_pattern="GACNNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    7273..7278
                     /Recognition_pattern="AGT^ACT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="AGTACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    7311..7322
                     /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)"
                     /Methylation="Dam:some blocked, CpG:some blocked"
                     /annotation_group="CGANNNNNNTGC"
                     /label="BcgI"
                     /note="Geneious type: restriction site"
     primer_bind     7345..7364
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(7583..7687)
                     /gene="bla"
                     /label="AmpR promoter"
     misc_feature    7597..7602
                     /Recognition_pattern="AAT^ATT"
                     /Methylation="EcoBI:cut"
                     /annotation_group="AATATT"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     misc_feature    7715..7
                     /Recognition_pattern="CG^TCGACG"
                     /annotation_group="CGTCGACG"
                     /label="SgrDI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag tcgaggtcga cggtatcgat
     2641 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
     2701 tatacgaagt tatattaagg gttccaagct taaaggctcc ggtgcccgtc agtgggcaga
     2761 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     2821 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     2881 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     2941 caacgggttt gccgccagaa cacaggatcc tcgagccacc atgattgaac aagatggatt
     3001 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3061 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3121 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     3181 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3241 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3301 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3361 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3421 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     3481 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     3541 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3601 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3661 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     3721 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctaactaga
     3781 ggtcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3841 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3901 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3961 gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggaaaa aggccagcaa
     4021 aaggccagga accgtaaaaa ggccgcgttg ctgcagtaac gccattttgc aaggcatgga
     4081 aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa
     4141 cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac
     4201 agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg
     4261 gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct
     4321 gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg
     4381 cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc gcgccagtcc
     4441 tccgattgac tgagtcgccc ggatcccgcc acccctgcag gtctagagtt taaacgaatt
     4501 cgtcgaggga cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg
     4561 gttccggttc cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta
     4621 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     4681 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     4741 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     4801 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4861 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4921 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4981 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     5041 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5101 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5161 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc
     5221 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     5281 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     5341 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5401 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     5461 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     5521 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     5581 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     5641 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     5701 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5881 cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7681 ttccgcgcac atttccccga aaagtgccac ctgac
//
