LOCUS       pAAV_hSyn_Spikey        6056 bp    DNA        circular     06-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   JP
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 6, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct"
                     /label="source:synthetic DNA construct"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="#00DF87"
                     /ApEinfo_revcolor="#00DF87"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden
     repeat_region   6..135
                     /note="Functional equivalent of wild-type AAV2 ITR"
                     /locus_tag="AAV2 ITR (alternate)"
                     /label="AAV2 ITR (alternate)"
                     /ApEinfo_label="AAV2 ITR (alternate)"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        180..627
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
                     /locus_tag="hSyn promoter"
                     /label="hSyn promoter"
                     /ApEinfo_label="hSyn promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    641..642
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    646..656
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    653..1936
                     /locus_tag="SpikeyGi2"
                     /label="SpikeyGi2"
                     /ApEinfo_label="SpikeyGi2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1105..1828
                     /locus_tag="cpsfGFP-OPT145-144"
                     /label="cpsfGFP-OPT145-144"
                     /ApEinfo_label="cpsfGFP-OPT145-144"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1106..1111
                     /locus_tag="DS_2AAins"
                     /label="DS_2AAins"
                     /ApEinfo_label="DS_2AAins"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1166..1168
                     /locus_tag="V172I"
                     /label="V172I"
                     /ApEinfo_label="V172I"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     variation       1844..1846
                     /locus_tag="R154Q"
                     /label="R154Q"
                     /ApEinfo_label="R154Q"
                     /ApEinfo_fwdcolor="#ff9e39"
                     /ApEinfo_revcolor="#ff9e39"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1943..1969
                     /locus_tag="GSS*3 linker"
                     /label="GSS*3 linker"
                     /ApEinfo_label="GSS*3 linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     variation       1976..2170
                     /locus_tag="Kv2.1 motif"
                     /label="Kv2.1 motif"
                     /ApEinfo_label="Kv2.1 motif"
                     /ApEinfo_fwdcolor="#ff9e39"
                     /ApEinfo_revcolor="#ff9e39"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2176..2177
                     /locus_tag="HindIII"
                     /label="HindIII"
                     /ApEinfo_label="HindIII"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2188..2776
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2241..2261)
                     /note="WPRE, reverse primer"
                     /locus_tag="WPRE-R"
                     /label="WPRE-R"
                     /ApEinfo_label="WPRE-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(2659..2670)
                     /codon_start="1"
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /locus_tag="Factor Xa site"
                     /label="Factor Xa site"
                     /ApEinfo_label="Factor Xa site"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    2808..3284
                     /note="human growth hormone polyadenylation signal"
                     /locus_tag="hGH poly(A) signal"
                     /label="hGH poly(A) signal"
                     /ApEinfo_label="hGH poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3007..3026)
                     /note="Human growth hormone terminator, reverse primer"
                     /locus_tag="hGH-PA-R"
                     /label="hGH-PA-R"
                     /ApEinfo_label="hGH-PA-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     repeat_region   3324..3464
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /locus_tag="AAV2 ITR"
                     /label="AAV2 ITR"
                     /ApEinfo_label="AAV2 ITR"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     repeat_region   3324..3453
                     /locus_tag="AAV2 ITR(1)"
                     /label="AAV2 ITR(1)"
                     /ApEinfo_label="AAV2 ITR"
                     /ApEinfo_fwdcolor="#0dfff7"
                     /ApEinfo_revcolor="#0dfff7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      3539..3994
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /locus_tag="f1 ori"
                     /label="f1 ori"
                     /ApEinfo_label="f1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3626..3645)
                     /note="F1 origin, reverse primer"
                     /locus_tag="F1ori-R"
                     /label="F1ori-R"
                     /ApEinfo_label="F1ori-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3836..3857
                     /note="F1 origin, forward primer"
                     /locus_tag="F1ori-F"
                     /label="F1ori-F"
                     /ApEinfo_label="F1ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(4011..4030)
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
                     /locus_tag="pRS-marker"
                     /label="pRS-marker"
                     /ApEinfo_label="pRS-marker"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     4130..4152
                     /note="pGEX vectors, reverse primer"
                     /locus_tag="pGEX 3'"
                     /label="pGEX 3'"
                     /ApEinfo_label="pGEX 3'"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(4190..4208)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
                     /locus_tag="pBRforEco"
                     /label="pBRforEco"
                     /ApEinfo_label="pBRforEco"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        4276..4380
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             4381..5241
                     /codon_start="1"
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(4599..4618)
                     /note="Ampicillin resistance gene, reverse primer"
                     /locus_tag="Amp-R"
                     /label="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      5412..6000
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5901..5920
                     /note="pBR322 origin, forward primer"
                     /locus_tag="pBR322ori-F"
                     /label="pBR322ori-F"
                     /ApEinfo_label="pBR322ori-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg cgtcgggcga
       61 cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca
      121 tcactagggg ttcctgcggc cgcacgcgtg tgtctagact gcagagggcc ctgcgtatga
      181 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
      241 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
      301 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
      361 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
      421 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
      481 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
      541 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
      601 ggagtcgtgt cgtgcctgag agcgcagtcg agaaggtacc ggatccgcca ccatggagac
      661 gactgtgagg tatgaacagg ggtcagagct cactaaaact tcgagctctc caacagcaga
      721 tgagcccacg ataaagattg atgatggtcg tgatgagggt aatgaacaag acagctgttc
      781 caataccatt aggagaaaaa tttccccgtt tgtgatgtca tttggattca gagtatttgg
      841 agttgtgctt atcattgtag acatcatagt ggtgattgtg gatctggcca tcagtgagaa
      901 gaaaagaggc attagagaga ttcttgaagg tgtttccctg gctatagcac tcttcttcct
      961 tgttgatgtt ctcatgagag tgtttgttga aggcttcaag aactatttcc ggtccaaact
     1021 gaatactttg gatgcagtca tagtagtggg cactctgcta attaatatga cctactcctt
     1081 ctctgacggt acatttcgcg gtgatgatag taacgtgtat attaccgcgg ataaacagaa
     1141 aaacggcatt aaagcgaact ttaccattcg ccataacgtg gaagatggca gcgtgcagct
     1201 ggcggatcat tatcagcaga acaccccgat tggcgatggc ccggtgctgc tgccggataa
     1261 ccattatctg agcacccaga ccgtgctgag caaagatccg aacgaaaaac gcgatcacat
     1321 ggtgctgctg gaatttgtga ccgcagcggg cattacacac ggcatggatg aactgtatgg
     1381 cggcaccggc ggcagcgcga gccagggcga agaactgttt accggcgtgg tgccgattct
     1441 ggtggaactg gatggcgatg tgaacggcca taaatttagc gtgcgcggcg aaggcgaagg
     1501 cgatgcgacc attggcaaac tgaccctgaa atttatttgc accaccggca aactaccggt
     1561 gccgtggccg accctggtga ccaccttaac ctatggcgtg cagtgcttta gccgctatcc
     1621 ggatcatatg aaacgccatg atttttttaa aagcgcgatg ccggaaggct atgtgcagga
     1681 acgcaccatt agctttaaag atgatggcaa atataaaacc cgcgcggtgg tgaaatttga
     1741 aggcgatacc ctggtgaacc gcattgaact gaaaggcacc gattttaaag aagatggcaa
     1801 cattctgggg cataaactgg aatataacac agatcagatg ccgcagatgg ttactcttct
     1861 tcgagttctg agaattgtta tcttaataag aatatttcag ctggcttcac agaagaaaca
     1921 acttgaagtg gtaaccctta agggttcttc aggttcatct ggatcttcca cgcgtcagag
     1981 ccagccgatt ctgaacacca aagaaatggc gccgcagagc aaaccgccgg aagaactgga
     2041 aatgagcagc atgccgagcc cggtggcgcc gctgccggcg cgcaccgaag gcgtgattga
     2101 tatgcgcagc atgagcagca ttgatagctt tattagctgc gcgaccgatt ttccggaagc
     2161 gacccgcttt tgataaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     2221 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2281 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2341 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2401 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2461 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2521 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2581 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     2641 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2701 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2761 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     2821 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     2881 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     2941 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3001 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     3061 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     3121 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     3181 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     3241 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     3301 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3361 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3421 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3481 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3541 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3601 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3661 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3721 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     3781 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3841 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     3901 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3961 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4021 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4081 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4141 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4201 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4261 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4321 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4381 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4441 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4501 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4561 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4621 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4681 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4741 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4801 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4861 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4921 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4981 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5041 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5101 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5161 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5221 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5281 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5341 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5401 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5461 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5521 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5581 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5641 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5701 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5761 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5821 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5881 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5941 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6001 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctca
//
