LOCUS       QC047-dCas12Max-        9860 bp DNA     circular SYN 20-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..241
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     226..249
                     /label=HQ1-F
     misc_feature    251..273
                     /label=crRNA
     misc_RNA        299..374
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(442..481)
                     /label=HQ1-R
     misc_feature    472..1265
                     /label=CBh
     enhancer        472..757
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        759..1036
                     /label=chicken beta-actin promoter
     intron          1037..1265
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     primer_bind     1231..1250
                     /label=HQ2-W1F
     primer_bind     1252..1276
                     /label=HQ2-F
     misc_feature    1252..1271
                     /label=HA
     CDS             1286..1339
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=bpNLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             1340..1837
                     /codon_start=1
                     /label=TadA8e
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQS
                     SIN"
     misc_feature    1652..1654
                     /label=106V
     misc_feature    1838..1939
                     /label=PEmax linker
     primer_bind     complement(1842..1910)
                     /label=HQ672-R
     CDS             1862..1915
                     /codon_start=1
                     /label=bpNLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             1895..1915
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1940..5179
                     /codon_start=1
                     /label=dxCas12i-v2.2
                     /translation="MSSDVVRPYNTKLLPDNRKHNMFLQTFKRLNSISLNHFDLLICLY
                     AAITNKKAEEYKSEKEAHVTADSLCAINWFRPMSKRYSKYATTTFNMLELFKEYSGHEP
                     DAYSKNYLMSNIDSDRFVWVDCRKFAKDFAYQMELGFHEFTVLAETLLANSILVLNEST
                     KANWAWGTVSALYGGGDKEDSTLKSKILLAFVDALNNHELKTKREILNQVCESLKYQSY
                     QDMYVDFRSVVDENGNKKSPRGSMPIVTKFETDDLISDNQRKAMISNFTKNAAAKAAKK
                     PIPYLDRLKEHMVSLCDEYNVYAWAAAITNSNADVTARNTRNLTFIGEQNSRRKRLSVL
                     QTTTNEKAKDILNKINDNLIQEVRYTPAPKHLGRDLANLFDTLKEKDINNIENEEEKQN
                     VINDCIEQYVDDCRSLNRNPIAALLKHISRYYEDFSAKNFLDGAKLNVLTEVVNRQKAH
                     PTIWSEKAYTWISKFDKNRRQANSSLVGWVVPPEEVHKEKIAGQQSMMWVTLTLLDDGK
                     WVKHHIPFSDSRYYSEVYAYNPNLPYLDGGIPRQSKFGNKPTTNLTAESQALLANSKYK
                     KANKSFLRAKENATHNVRVSPNTSLCIRLLKDSAGNQMFDKIGNVLFGMQINHKITVGK
                     PNYKIEVGDRFLGFDQNQSENHTYAVLQRVSESSHDTHHFNGWDVKVLEKGKVTSDVIV
                     RDEVYDQLSYEGVPYDSSKFAEWRDKRRRFVLENLSIQLEEGKTFLTEFDKLNKDSLYR
                     WNMNYLKLLRKAIRAGGKEFAKIAKTEIFELAVERFGPINLGSLSQISLKMIASFKGVV
                     QSYFSVSGCVDDASKKAHDSMLFTFMCAAEEKRTNKREEKTNRAASFILQKAYLHGCKM
                     IVCEDDLPVADGKTGKAQNADRMDWCARALAKKVNDGCVAMSICYRAIPAYMSSHQDPF
                     VHMQDKKTSVLRPRFMEVNKDSIRDYHVAGLRRMLNSKSDAGTSVYYRQAALHFCEALG
                     VSPELVKNKKTHAAELGKHMGSAMLMPWRGGRVYIASKKLTSDAKSVKYCGEDMWQYHA
                     AEIAAVNIAMYEVCCQTGAFGKKQKKSDELPG"
     misc_feature    2666..2668
                     /label=N243R
     misc_feature    2945..2947
                     /label=E336R
     primer_bind     3437..3456
                     /label=12i16-W3F
     primer_bind     complement(3558..3577)
                     /label=12i-BbJD-W1R
     primer_bind     complement(3869..3901)
                     /label=HEKpr011
     primer_bind     3877..3910
                     /label=HEKpr012
     primer_bind     complement(4015..4048)
                     /label=HEKpr008
     primer_bind     4028..4059
                     /label=HEKpr009
     primer_bind     4196..4215
                     /label=12i16-W4F
     primer_bind     4324..4344
                     /label=HQ522-F
     primer_bind     complement(4340..4367)
                     /label=HQ2-R
     primer_bind     complement(4376..4395)
                     /label=HQ2-W1R
     primer_bind     complement(4543..4577)
                     /label=HQ523-R
     primer_bind     4552..4585
                     /label=HQ523-F
     primer_bind     complement(5063..5097)
                     /label=HEKpr015
     primer_bind     5074..5107
                     /label=HEKpr016
     CDS             5180..5227
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5233..5255)
                     /label=HQ522-R
     misc_feature    5241..5255
                     /label=HA
     polyA_signal    5261..5468
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5537..5840
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5841..6044
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6073..6783
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     rep_origin      7338..7793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8075..8179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8180..9040
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8572..8600
                     /label=HNZ1295-F
     rep_origin      9211..9799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg agaaatgtgt ccccagttga cacagagacc gagaggtctc gtttttttgt
      301 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      361 caccgagtcg gtgctttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
      421 ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagaggtac ccgttacata
      481 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      541 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      601 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      661 cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact ttcctacttg
      721 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      781 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      841 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      901 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      961 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     1021 aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg
     1081 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1141 gggacggccc ttctcctccg ggctgtaatt agctgagcaa gaggtaaggg tttaagggat
     1201 ggttggttgg tggggtatta atgtttaatt acctggagca cctgcctgaa atcacttttt
     1261 ttcaggttgg accggtgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
     1321 aaagaagaag cggaaagtct ctgaggtgga gttttcccac gagtactgga tgagacatgc
     1381 cctgaccctg gccaagaggg cacgggatga gagggaggtg cctgtgggag ccgtgctggt
     1441 gctgaacaat agagtgatcg gcgagggctg gaacagagcc atcggcctac acgacccaac
     1501 agcccatgcc gaaattatgg ccctgagaca aggcggcctg gtcatgcaga actacagact
     1561 gattgacgcc accctgtacg tgacattcga gccttgcgtg atgtgcgctg gcgccatgat
     1621 ccactctagg atcggccgcg tggtgtttgg agtgagaaat tctaaaagag gcgccgcagg
     1681 ctccctgatg aacgtgctga actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg
     1741 aatcctggca gatgaatgtg ccgccctgct gtgcgacttc tatcggatgc ctagacaggt
     1801 gttcaatgct cagaagaagg cccagagttc catcaactcc ggaggatcta gcggaggctc
     1861 caagagaacc gctgatggct cagagtttga gagcccaaaa aagaaaagaa aagtctctgg
     1921 cggaagctcc gggggctcta tgagttctga tgtggtgcgg ccttataaca caaagctgct
     1981 cccagataac agaaagcaca atatgttcct gcagaccttc aagcggctga acagcatctc
     2041 tctgaaccac ttcgacctgc tgatctgcct gtacgctgca atcaccaaca agaaggccga
     2101 ggaatacaag tctgaaaagg aagcccacgt gaccgccgat agcctgtgtg ccatcaattg
     2161 gttcagaccc atgagcaaga gatacagcaa atacgccacc accaccttca acatgttaga
     2221 actgtttaag gagtacagcg gccacgagcc tgatgcctat tccaagaact acctgatgag
     2281 caatatcgac agcgacagat tcgtgtgggt ggattgtagg aagttcgcta aggactttgc
     2341 ctatcagatg gaactgggtt tccacgagtt caccgtgttg gccgaaaccc tgctggctaa
     2401 ttctatcctg gtgctgaacg agagcaccaa ggccaattgg gcttggggaa ccgtgtctgc
     2461 cctgtacggc ggcggagata aggaggacag cacactgaag agcaagattc tgctggcctt
     2521 cgtggacgcc ctgaacaacc acgagctgaa aacaaagaga gaaatcttga atcaagtgtg
     2581 tgaatctctg aaataccaga gctaccagga catgtacgtg gattttagaa gcgtggttga
     2641 cgaaaacggc aacaagaagt ctcctagagg ctctatgcct atcgtgacca agttcgagac
     2701 agacgacctg atcagcgaca accaaagaaa ggccatgatc agcaacttca ctaagaacgc
     2761 cgctgccaag gcagctaaga aacctatccc ttacttggac cgcctgaagg agcacatggt
     2821 gtccctgtgc gacgagtaca atgtgtatgc ctgggccgcg gccatcacaa acagcaacgc
     2881 cgacgtgacc gcccggaata ccagaaacct gacattcatc ggcgaacaga acagcagacg
     2941 aaagagactg agcgtgctgc agacaacaac caacgagaag gctaaggaca tcctgaacaa
     3001 gatcaacgac aacctgattc aggaggtgcg gtacacccct gcccctaagc acctgggcag
     3061 agatctggcc aacctgtttg atacactgaa ggaaaaggac atcaacaaca tcgagaacga
     3121 agaagagaaa cagaacgtga tcaatgactg tatcgagcag tacgtggacg attgcagaag
     3181 cctcaaccgg aaccccatcg cagccctcct gaagcacatc tctaggtact acgaggattt
     3241 cagcgccaag aatttcctgg acggcgccaa gctgaacgtg ctgactgagg tggtgaaccg
     3301 gcagaaggcc caccccacca tctggagcga gaaggcttac acctggatca gcaagttcga
     3361 caagaaccgg agacaggcca acagcagcct ggtcggatgg gttgtgcccc ccgaggaggt
     3421 gcacaaggag aaaatcgccg gacagcagag catgatgtgg gtgaccctca ccctgctgga
     3481 cgacggcaag tgggtcaaac atcacatccc cttcagcgac agcagatact acagcgaagt
     3541 gtacgcctac aaccctaatc tgccttatct ggacggaggc atcccaagac agagcaagtt
     3601 cggcaacaaa ccaacaacca acctgacagc cgagtcccag gccctcctgg ctaattctaa
     3661 gtacaagaaa gccaacaaga gcttcctgcg ggctaaagag aatgccacac acaacgtgcg
     3721 ggtgtcccct aacacctctc tgtgcattag actgctgaag gacagcgccg gaaaccagat
     3781 gttcgacaaa atcggcaacg tgctcttcgg catgcagatc aaccacaaga tcaccgtggg
     3841 aaaacctaac tacaagatcg aggtgggcga cagattcctg ggcttcgatc agaaccagag
     3901 cgagaaccac acctacgccg tgctgcagag agtgtccgag agcagtcacg acacccacca
     3961 ctttaacggc tgggacgtga aggtgctgga aaagggcaaa gtgaccagcg atgtgatcgt
     4021 gcgggacgag gtctacgacc aactgtctta cgagggcgtc ccctacgata gcagcaagtt
     4081 cgccgagtgg cgggacaagc gcagaagatt tgtgcttgag aacctgagca tccagctgga
     4141 agagggcaag accttcctga cagagttcga caagctgaat aaggacagcc tgtaccgctg
     4201 gaacatgaac tacctgaaac tgctgagaaa ggccatccgg gccggaggca aagagttcgc
     4261 caagatcgct aagacagaga tcttcgagct ggcggtggaa agattcggcc ctattaacct
     4321 gggcagcctg tcccagatca gccttaagat gattgcctcc tttaagggcg tggtccagtc
     4381 ctacttctcc gtgagcggct gcgtggatga tgcctccaaa aaggcccatg attctatgct
     4441 gttcacattt atgtgcgccg ccgaagaaaa gcggaccaac aagagagaag aaaagaccaa
     4501 cagagccgcc agctttatcc tgcaaaaagc ctacctgcat ggctgcaaga tgatcgtgtg
     4561 cgaggacgac cttcctgtgg ccgacggcaa gacaggcaaa gcccagaatg ccgaccggat
     4621 ggactggtgc gccagagccc tggccaagaa ggtgaacgac ggctgtgttg ccatgagcat
     4681 ctgctacaga gctatccctg cctacatgag cagccaccag gacccctttg tgcacatgca
     4741 ggataagaaa accagcgtgc tgcggcctag attcatggaa gttaataagg atagcatcag
     4801 agactaccac gtggcgggcc tgagaagaat gctgaacagc aagagtgacg ctggcaccag
     4861 tgtttattac cggcaagctg ccctgcattt ctgcgaagcc ctgggcgtga gccctgaact
     4921 ggtgaaaaac aagaaaaccc acgccgccga actgggcaag cacatgggca gcgctatgct
     4981 gatgccctgg agaggcggta gagtgtacat cgccagcaaa aagctgacct ccgatgccaa
     5041 atcagtgaag tactgcggcg aggatatgtg gcagtaccac gccgccgaga tcgccgctgt
     5101 taacatcgcc atgtatgagg tgtgctgcca gaccggcgct ttcggaaaga aacagaaaaa
     5161 atcggacgag ctgcctggaa aaaggccggc ggccacgaag aaggccggcc aggcaaagaa
     5221 gaagaagtaa gaattcctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     5281 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5341 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5401 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat
     5461 gctggggagc ggccgctagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     5521 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5581 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5641 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5701 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5761 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     5821 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     5881 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5941 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     6001 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
     6061 agatccgcta gcatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     6121 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     6181 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     6241 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     6301 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     6361 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     6421 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     6481 gacggccccg taatgcagaa gaaaaccatg ggctgggagg cctcctccga gcggatgtac
     6541 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     6601 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     6661 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     6721 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     6781 taaggatccg ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg
     6841 gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat
     6901 ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc ctgctcttta
     6961 ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa
     7021 caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat agatctatag
     7081 atctctcgtg ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg
     7141 tttccaaatg tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata
     7201 cacttcattc tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgacctgc
     7261 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     7321 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7381 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7441 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7501 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     7561 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7621 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     7681 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7741 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     7801 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     7861 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     7921 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     7981 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     8041 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8101 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8161 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8221 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8281 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8341 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8401 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8461 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8521 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8581 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8641 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8701 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8761 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8821 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8881 agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8941 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9001 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9061 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9121 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     9361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9601 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9841 ctggcctttt gctcacatgt
//
