LOCUS       QC067-dCas12Max-       10274 bp DNA     circular SYN 05-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10274)
  AUTHORS   Star
  TITLE     Direct Submission
  JOURNAL   Exported Dec 6, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 Promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     246..298
                     /label=HQ3-F
     primer_bind     complement(250..302)
                     /label=HQ3-R
     misc_feature    251..273
                     /label=crRNA
     misc_RNA        299..374
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     primer_bind     complement(457..476)
                     /label=qq-pr615-R
     misc_feature    472..1265
                     /label=CBh
     misc_feature    472..757
                     /label=CMV enhancer
     promoter        759..1036
                     /label=chicken beta-actin promoter
     misc_feature    1037..1265
                     /label=Hyrid Intron
     primer_bind     1252..1276
                     /label=Primer 5
     misc_feature    1272..1275
                     /label=Feature 21
     CDS             1283..1339
                     /codon_start=1
                     /label=bpNLS
                     /translation="MKRTADGSEFESPKKKRKV"
     CDS             1340..1936
                     /codon_start=1
                     /label=A3A(BEACON2) W104A
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISYSPCFSA
                     GCAGEVRAFLQENTHVRLRIFAARIFDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     primer_bind     complement(1916..1946)
                     /label=Primer 4
     primer_bind     1927..1956
                     /label=Primer 1
     misc_feature    1937..2038
                     /label=GS-BPNLS-GS linker
     primer_bind     complement(1941..2009)
                     /label=HQ672-R
     CDS             1961..2014
                     /codon_start=1
                     /label=bpNLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             1994..2014
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2039..5278
                     /codon_start=1
                     /label=dxCas12i-v2.2
                     /translation="MSSDVVRPYNTKLLPDNRKHNMFLQTFKRLNSISLNHFDLLICLY
                     AAITNKKAEEYKSEKEAHVTADSLCAINWFRPMSKRYSKYATTTFNMLELFKEYSGHEP
                     DAYSKNYLMSNIDSDRFVWVDCRKFAKDFAYQMELGFHEFTVLAETLLANSILVLNEST
                     KANWAWGTVSALYGGGDKEDSTLKSKILLAFVDALNNHELKTKREILNQVCESLKYQSY
                     QDMYVDFRSVVDENGNKKSPRGSMPIVTKFETDDLISDNQRKAMISNFTKNAAAKAAKK
                     PIPYLDRLKEHMVSLCDEYNVYAWAAAITNSNADVTARNTRNLTFIGEQNSRRKRLSVL
                     QTTTNEKAKDILNKINDNLIQEVRYTPAPKHLGRDLANLFDTLKEKDINNIENEEEKQN
                     VINDCIEQYVDDCRSLNRNPIAALLKHISRYYEDFSAKNFLDGAKLNVLTEVVNRQKAH
                     PTIWSEKAYTWISKFDKNRRQANSSLVGWVVPPEEVHKEKIAGQQSMMWVTLTLLDDGK
                     WVKHHIPFSDSRYYSEVYAYNPNLPYLDGGIPRQSKFGNKPTTNLTAESQALLANSKYK
                     KANKSFLRAKENATHNVRVSPNTSLCIRLLKDSAGNQMFDKIGNVLFGMQINHKITVGK
                     PNYKIEVGDRFLGFDQNQSENHTYAVLQRVSESSHDTHHFNGWDVKVLEKGKVTSDVIV
                     RDEVYDQLSYEGVPYDSSKFAEWRDKRRRFVLENLSIQLEEGKTFLTEFDKLNKDSLYR
                     WNMNYLKLLRKAIRAGGKEFAKIAKTEIFELAVERFGPINLGSLSQISLKMIASFKGVV
                     QSYFSVSGCVDDASKKAHDSMLFTFMCAAEEKRTNKREEKTNRAASFILQKAYLHGCKM
                     IVCEDDLPVADGKTGKAQNADRMDWCARALAKKVNDGCVAMSICYRAIPAYMSSHQDPF
                     VHMQDKKTSVLRPRFMEVNKDSIRDYHVAGLRRMLNSKSDAGTSVYYRQAALHFCEALG
                     VSPELVKNKKTHAAELGKHMGSAMLMPWRGGRVYIASKKLTSDAKSVKYCGEDMWQYHA
                     AEIAAVNIAMYEVCCQTGAFGKKQKKSDELPG"
     primer_bind     2208..2227
                     /label=HEKpr357
     misc_feature    2765..2767
                     /label=N243R
     primer_bind     complement(3028..3054)
                     /label=E336R_R
     primer_bind     3039..3067
                     /label=E336R_F
     misc_feature    3044..3046
                     /label=E336R
     primer_bind     complement(3145..3172)
                     /label=D375R_R
     primer_bind     3164..3186
                     /label=D375R_F
     primer_bind     complement(3968..4000)
                     /label=HEKpr011
     primer_bind     3976..4009
                     /label=HEKpr012
     primer_bind     complement(4114..4147)
                     /label=HEKpr008
     primer_bind     4127..4158
                     /label=HEKpr009
     primer_bind     complement(4439..4466)
                     /label=HQ2-R
     primer_bind     complement(4642..4676)
                     /label=HEKpr013
     primer_bind     4651..4684
                     /label=HEKpr014
     primer_bind     complement(5073..5091)
                     /label=HEKpr358
     primer_bind     complement(5162..5196)
                     /label=HEKpr015
     primer_bind     5173..5206
                     /label=HEKpr016
     primer_bind     complement(5261..5278)
                     /label=HEKpr010
     CDS             5285..5305
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5318..5566
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     misc_feature    5591..5641
                     /label=bp-NLS
     primer_bind     complement(5617..5644)
                     /label=Primer 2
     misc_feature    5646..5649
                     /label=Feature 22
     polyA_signal    5675..5882
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5951..6254
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6255..6458
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6466..6502
                     /label=Vy180-T1-F
     CDS             6487..7197
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6896..6933)
                     /label=Vy180-T1-R
     primer_bind     6896..6933
                     /label=Vy180-T2-F
     CDS             6918
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation=""
     primer_bind     complement(7178..7214)
                     /label=Vy180-T2-R
     rep_origin      7752..8207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8489..8593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8594..9454
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8995..9014
                     /label=qq-pr614-F
     rep_origin      9625..10213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg agaaatgtgt ccccagttga cacagagacc gagaggtctc gtttttttgt
      301 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      361 caccgagtcg gtgctttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
      421 ccgtttttag cgcgtgcgcc aattctgcag acaaatggct ctagaggtac ccgttacata
      481 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      541 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      601 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      661 cggtaaatgg cccgcctggc attgtgccca gtacatgacc ttatgggact ttcctacttg
      721 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      781 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      841 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      901 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      961 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     1021 aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg
     1081 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1141 gggacggccc ttctcctccg ggctgtaatt agctgagcaa gaggtaaggg tttaagggat
     1201 ggttggttgg tggggtatta atgtttaatt acctggagca cctgcctgaa atcacttttt
     1261 ttcaggttgg accggtgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
     1321 aaagaagaag cggaaagtca tggaggcatc tccagcaagc ggaccaaggc acctgatgga
     1381 cccccacatc ttcacctcta actttaacaa tggcatcggc aggcacaaga catacctgtg
     1441 ctatgaggtg gagcgcctgg acaatggcac cagcgtgaag atggatcagc acagaggctt
     1501 cctgcacaac caggccaaga atctgctgtg cggcttctac ggccggcacg cagagctgag
     1561 atttctggac ctggtgccta gcctgcagct ggatccagcc cagatctata gggtgacctg
     1621 gttcatcagc tactccccat gcttttccgc gggatgtgca ggagaggtgc gcgccttcct
     1681 gcaggagaac acacacgtgc ggctgagaat ctttgccgcc cggatcttcg actatgatcc
     1741 tctgtacaag gaggccctgc agatgctgag agacgcagga gcccaggtgt ccatcatgac
     1801 ctatgatgag ttcaagcact gctgggacac atttgtggat caccagggct gtccctttca
     1861 gccttgggac ggactggatg agcactccca ggccctgtct ggcaggctga gggccatcct
     1921 gcagaaccag ggcaattccg gaggatctag cggaggctcc aagagaaccg ctgatggctc
     1981 agagtttgag agcccaaaaa agaaaagaaa agtctctggc ggaagctccg ggggctctat
     2041 gagttctgat gtggtgcggc cttataacac aaagctgctc ccagataaca gaaagcacaa
     2101 tatgttcctg cagaccttca agcggctgaa cagcatctct ctgaaccact tcgacctgct
     2161 gatctgcctg tacgctgcaa tcaccaacaa gaaggccgag gaatacaagt ctgaaaagga
     2221 agcccacgtg accgccgata gcctgtgtgc catcaattgg ttcagaccca tgagcaagag
     2281 atacagcaaa tacgccacca ccaccttcaa catgttagaa ctgtttaagg agtacagcgg
     2341 ccacgagcct gatgcctatt ccaagaacta cctgatgagc aatatcgaca gcgacagatt
     2401 cgtgtgggtg gattgtagga agttcgctaa ggactttgcc tatcagatgg aactgggttt
     2461 ccacgagttc accgtgttgg ccgaaaccct gctggctaat tctatcctgg tgctgaacga
     2521 gagcaccaag gccaattggg cttggggaac cgtgtctgcc ctgtacggcg gcggagataa
     2581 ggaggacagc acactgaaga gcaagattct gctggccttc gtggacgccc tgaacaacca
     2641 cgagctgaaa acaaagagag aaatcttgaa tcaagtgtgt gaatctctga aataccagag
     2701 ctaccaggac atgtacgtgg attttagaag cgtggttgac gaaaacggca acaagaagtc
     2761 tcctagaggc tctatgccta tcgtgaccaa gttcgagaca gacgacctga tcagcgacaa
     2821 ccaaagaaag gccatgatca gcaacttcac taagaacgcc gctgccaagg cagctaagaa
     2881 acctatccct tacttggacc gcctgaagga gcacatggtg tccctgtgcg acgagtacaa
     2941 tgtgtatgcc tgggccgcgg ccatcacaaa cagcaacgcc gacgtgaccg cccggaatac
     3001 cagaaacctg acattcatcg gcgaacagaa cagcagacga aagagactga gcgtgctgca
     3061 gacaacaacc aacgagaagg ctaaggacat cctgaacaag atcaacgaca acctgattca
     3121 ggaggtgcgg tacacccctg cccctaagca cctgggcaga gatctggcca acctgtttga
     3181 tacactgaag gaaaaggaca tcaacaacat cgagaacgaa gaagagaaac agaacgtgat
     3241 caatgactgt atcgagcagt acgtggacga ttgcagaagc ctcaaccgga accccatcgc
     3301 agccctcctg aagcacatct ctaggtacta cgaggatttc agcgccaaga atttcctgga
     3361 cggcgccaag ctgaacgtgc tgactgaggt ggtgaaccgg cagaaggccc accccaccat
     3421 ctggagcgag aaggcttaca cctggatcag caagttcgac aagaaccgga gacaggccaa
     3481 cagcagcctg gtcggatggg ttgtgccccc cgaggaggtg cacaaggaga aaatcgccgg
     3541 acagcagagc atgatgtggg tgaccctcac cctgctggac gacggcaagt gggtcaaaca
     3601 tcacatcccc ttcagcgaca gcagatacta cagcgaagtg tacgcctaca accctaatct
     3661 gccttatctg gacggaggca tcccaagaca gagcaagttc ggcaacaaac caacaaccaa
     3721 cctgacagcc gagtcccagg ccctcctggc taattctaag tacaagaaag ccaacaagag
     3781 cttcctgcgg gctaaagaga atgccacaca caacgtgcgg gtgtccccta acacctctct
     3841 gtgcattaga ctgctgaagg acagcgccgg aaaccagatg ttcgacaaaa tcggcaacgt
     3901 gctcttcggc atgcagatca accacaagat caccgtggga aaacctaact acaagatcga
     3961 ggtgggcgac agattcctgg gcttcgatca gaaccagagc gagaaccaca cctacgccgt
     4021 gctgcagaga gtgtccgaga gcagtcacga cacccaccac tttaacggct gggacgtgaa
     4081 ggtgctggaa aagggcaaag tgaccagcga tgtgatcgtg cgggacgagg tctacgacca
     4141 actgtcttac gagggcgtcc cctacgatag cagcaagttc gccgagtggc gggacaagcg
     4201 cagaagattt gtgcttgaga acctgagcat ccagctggaa gagggcaaga ccttcctgac
     4261 agagttcgac aagctgaata aggacagcct gtaccgctgg aacatgaact acctgaaact
     4321 gctgagaaag gccatccggg ccggaggcaa agagttcgcc aagatcgcta agacagagat
     4381 cttcgagctg gcggtggaaa gattcggccc tattaacctg ggcagcctgt cccagatcag
     4441 ccttaagatg attgcctcct ttaagggcgt ggtccagtcc tacttctccg tgagcggctg
     4501 cgtggatgat gcctccaaaa aggcccatga ttctatgctg ttcacattta tgtgcgccgc
     4561 cgaagaaaag cggaccaaca agagagaaga aaagaccaac agagccgcca gctttatcct
     4621 gcaaaaagcc tacctgcatg gctgcaagat gatcgtgtgc gaggacgacc ttcctgtggc
     4681 cgacggcaag acaggcaaag cccagaatgc cgaccggatg gactggtgcg ccagagccct
     4741 ggccaagaag gtgaacgacg gctgtgttgc catgagcatc tgctacagag ctatccctgc
     4801 ctacatgagc agccaccagg acccctttgt gcacatgcag gataagaaaa ccagcgtgct
     4861 gcggcctaga ttcatggaag ttaataagga tagcatcaga gactaccacg tggcgggcct
     4921 gagaagaatg ctgaacagca agagtgacgc tggcaccagt gtttattacc ggcaagctgc
     4981 cctgcatttc tgcgaagccc tgggcgtgag ccctgaactg gtgaaaaaca agaaaaccca
     5041 cgccgccgaa ctgggcaagc acatgggcag cgctatgctg atgccctgga gaggcggtag
     5101 agtgtacatc gccagcaaaa agctgacctc cgatgccaaa tcagtgaagt actgcggcga
     5161 ggatatgtgg cagtaccacg ccgccgagat cgccgctgtt aacatcgcca tgtatgaggt
     5221 gtgctgccag accggcgctt tcggaaagaa acagaaaaaa tcggacgagc tgcctggagg
     5281 cagccccaag aagaagcgga aggtgtctgg tggttctacc aacctgtctg acatcatcga
     5341 gaaggagaca ggcaagcagc tggtcatcca ggagagcatc ctgatgctgc cagaggaggt
     5401 ggaggaagtg atcggcaaca agcccgagtc cgatatcctg gtgcacaccg cctacgacga
     5461 gagcacagat gagaatgtga tgctgctgac ctccgacgcc cccgagtata agccttgggc
     5521 cctggtcatc caggattcta acggcgagaa taagatcaag atgctgtctg gtggttcttc
     5581 tggcggctca aagagaaccg ccgacggcag cgaatccgag cccaagaaga agaggaaagt
     5641 ctaagaattc ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5701 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5761 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5821 ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg
     5881 gagcggccgc tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     5941 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     6001 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     6061 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     6121 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     6181 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     6241 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     6301 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     6361 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     6421 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
     6481 gctagcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     6541 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     6601 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     6661 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     6721 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     6781 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     6841 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     6901 cccgtaatgc agaagaaaac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     6961 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     7021 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     7081 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     7141 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagga
     7201 tccgggatgc agaaattgat gatctattaa acaataaaga tgtccactaa aatggaagtt
     7261 tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt gaatggaagg
     7321 attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc tttactgaag
     7381 gctctttact attgctttat gataatgttt catagttgga tatcataatt taaacaagca
     7441 aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct atagatctct
     7501 cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact gtggtttcca
     7561 aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca catacacttc
     7621 attctcagta ttgttttgcc aagttctaat tccatcagaa gctggtcgac ctgcaggggc
     7681 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     7741 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7801 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7861 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7921 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     7981 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8041 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     8101 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8161 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     8221 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     8281 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     8341 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     8401 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     8461 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8521 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8581 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8641 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8701 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8761 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8821 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8881 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8941 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9001 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9061 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9121 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9181 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9241 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9301 tgagcgtgga agccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9361 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9421 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9481 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9541 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9601 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9661 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9721 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     9781 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9841 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9901 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9961 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10021 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10081 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10141 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10201 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10261 ttttgctcac atgt
//
