LOCUS       pME166-1_01              3129 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1032..1826
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      join(3108..3129,1..728)
                     /note="pLannotate"
                     /label="RSF ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1979..2695
                     /note="pLannotate"
                     /label="EGFP"
                     /database="addgene"
                     /identity="99.721"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(2947..3033)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="addgene"
                     /identity="98.864"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      818..863
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        1918..1952
                     /note="pLannotate"
                     /label="J23119 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      1853..1884
                     /note="pLannotate"
                     /label="tonB terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             2708..2728
                     /note="pLannotate"
                     /label="SV40 NLS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     ncRNA           2828..2942
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="infernal"
                     /identity="100.0"
                     /match_length="97.47899159663865"
                     /fragment="False"
                     /other="ncRNA"
ORIGIN
        1 ttgatagacc gattgattca tcatctcata aataaagaaa aaccaccgct accaacggtg
       61 gttttctcaa ggttcgctga gctaccaact ctttgaacca aggtaagtgg gttggaggac
      121 cgcactcacc aaaatctgtt ctttcagttt agccttaaca ggtgcataac ttcaagacaa
      181 agtcctctaa atcagttacc aatggctgct gccagtggcg ataagtcgtg tcttaccggg
      241 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      301 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca acagcgtgag
      361 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      421 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      481 agtcctgtcg ggtttcgcca cctctggctt gagcgtcgat ttttgtgatg ctcgtcaggg
      541 gggcggagcc tatggaaaaa cgcctgcggc gttggcttct tccggtgctt tgctttttgc
      601 tcacatgttc tttccggctt tatcccctga ttctgtggat aaccgtatta ccgcttttga
      661 gtgagctgac accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
      721 agcggaagtt ttctactgaa ccgctctaga tttcagtgca atttatctct tcaaatgtag
      781 cacctgaagt cagcccagga ggaagaggac atccggtcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttagacttag ggaccctaag ccaaataggc cgtccggaat
      901 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
      961 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag
     1021 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     1081 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     1141 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     1201 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     1261 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     1321 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     1381 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     1441 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     1501 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     1561 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     1621 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     1681 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     1741 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     1801 atcgccttct tgacgagttc ttctaaactt aattaacggc actcctcagc caagtcaaaa
     1861 gcctccgacc ggaggctttt gactacatgc ccatggcgtt gtctgattcg ttaccaattg
     1921 acagctagct cagtcctagg tataatgcta gctactagtg aaagaggaga aatactagat
     1981 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2041 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2101 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2161 cgtgaccacc ctgacctatg gagtgcagtg cttcagccgc taccccgacc acatgaagca
     2221 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2281 caaggacgac ggcaactaca agacccgcgt agaggtgaag ttcgagggcg acaccctggt
     2341 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2401 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2461 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2521 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2581 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2641 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtctgg
     2701 tggttctccc aagaagaaaa ggaaagtcta agaattcgaa gcttggctgt tttggcggat
     2761 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     2821 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     2881 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     2941 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3001 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaaaagccaa
     3061 ataggccgtc actcggtcgc tacgctccgg gcgtgagact gcggcggaac ggaatagctg
     3121 ttcgttgac
//
