LOCUS       Exported                7920 bp DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7920
                     /label=dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)
                     /note="dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)"
                     /organism="synthetic DNA construct"
     source          2461..3172
                     /label=dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)
                     /note="dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)"
                     /organism="synthetic DNA construct"
     source          3317..6997
                     /note="/dnas_title=pYPQ166"
                     /organism="synthetic DNA construct"
     source          3317..6997
                     /label=dnas_title=AsCpf1
                     /note="dnas_title=AsCpf1"
     source          3317..6997
                     /label=dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)
                     /note="dnas_title=pYPQ255 (APOBEC-pcoCas9n-UGI)"
                     /organism="synthetic DNA construct"
     source          6998..7362
                     /label=dnas_title=AsCpf1
                     /note="dnas_title=AsCpf1"
     source          6998..7045
                     /note="/dnas_title=pYPQ166"
                     /organism="synthetic DNA construct"
     promoter        1..138
                     /note="Spn promoter
                     vntifkey=30
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=Spn promoter
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     CDS             139..1149
                     /codon_start=1
                     /gene="Streptomycin/Spectinomycin adenyltransferase"
                     /label=Streptomycin/Spectinomycin adenyltransferase
                     /note="SpnR
                     vntifkey=4
                     ApEinfo_revcolor=pink
                     invitrogen=2750000
                     ApEinfo_fwdcolor=pink
                     dnas_title=SpnR
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1216..1889
                     /gene="ori"
                     /label=pUC origin
                     /note="pUC origin
                     vntifkey=33
                     ApEinfo_revcolor=pink
                     invitrogen=2530000
                     ApEinfo_fwdcolor=pink
                     dnas_title=pUC origin
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     terminator      2319..2362
                     /note="rrnB T1 terminatior
                     vntifkey=43
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=rrnB T1 terminatior 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     misc_recomb     2461..2560
                     /label=attL1
                     /note="attL1
                     vntifkey=86
                     ApEinfo_revcolor=pink
                     invitrogen=2670000
                     ApEinfo_fwdcolor=#00ff40
                     dnas_title=attL1
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     CDS             2576..3172
                     /codon_start=1
                     /product="Rice codon optimized"
                     /label=hA3A
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIFDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     misc_feature    2963..2965
                     /product="Mutation to narrow base editing window"
                     /label=Y130F
     terminator      3173..3262
                     /label=Linker1
     CDS             3173..3262
                     /codon_start=1
                     /label=Mb2Cas12a-BE
                     /translation="GGGGSGGGGSGGGGSGGGGSGGGGSGGGGS"
     gene            3266..3316
                     /label=N-terminal NLS
                     /note="N-terminal NLS"
     misc_feature    3272..3292
                     /label=SV40 NLS
                     /note="SV40 NLS"
     gene            3317..6997
                     /label=LbCpf1
                     /note="LbCpf1"
     misc_feature    3744..3767
                     /label=LbCpf1-seq1
                     /note="""LbCpf1-seq1"
     misc_feature    3779..3781
                     /label=D156R
     misc_feature    6998..7045
                     /label=nucleoplasmin NLS
     misc_feature    7055..7348
                     /label=UGI-NLS
                     /note="UGI-NLS"
     misc_feature    7368..7645
                     /label=NOS term
                     /note="NOS term
                     vntifkey=21
                     ApEinfo_revcolor=green
                     ApEinfo_fwdcolor=#f5010a
                     dnas_title=NOS term
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     misc_recomb     7667..7790
                     /label=attR5
                     /note="attR5
                     vntifkey=86
                     ApEinfo_revcolor=pink
                     invitrogen=2703000
                     ApEinfo_fwdcolor=#00ff00
                     dnas_title=attR5
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     promoter        7919..7920
                     /note="Spn promoter
                     vntifkey=30
                     ApEinfo_revcolor=pink
                     ApEinfo_fwdcolor=pink
                     dnas_title=Spn promoter
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgga
     2581 ggcctctccg gcttcaggtc ctcgccatct catggacccc catatcttta cctccaattt
     2641 caataacggc attggcaggc acaagacata cctctgttat gaggtcgagc ggctcgataa
     2701 cggaacctca gtgaagatgg accaacacag gggtttcctt cataaccaag cgaaaaatct
     2761 cctttgcggt ttctatgggc ggcacgcgga gctgagattc ttggatttgg tcccgagcct
     2821 ccagctggat cccgcgcaaa tataccgcgt cacttggttc atatcatgga gcccgtgctt
     2881 tagctggggg tgtgccggtg aggttcgcgc gttcctccag gaaaacaccc atgttcgcct
     2941 ccggattttc gcagcgagga ttttcgacta tgatccactt tacaaagagg ccctccaaat
     3001 gcttagagat gctggggctc aagtttcaat tatgacatac gacgagttta agcattgttg
     3061 ggacactttt gtcgatcatc agggttgtcc cttccagccg tgggatggtc ttgatgagca
     3121 ttcgcaagct ttgagtggca ggcttcgcgc gatacttcag aaccagggta acgggggcgg
     3181 gggctcaggc gggggcggga gcggcggcgg gggctctggg ggcggcggca gcggcggggg
     3241 cggcagcggg ggcggcgggt cgaccatggc tcctaagaag aagcggaagg ttggtattca
     3301 cggggtgcct gcggcttcaa agctcgagaa attcaccaac tgttattcgt tgagcaaaac
     3361 actgcggttt aaagcgattc cagtcggcaa gactcaagag aatatagaca ataagcggct
     3421 gttggtggaa gatgaaaagc gcgcggaaga ctacaaaggg gtgaagaagt tgttggacag
     3481 atactacctc tcttttatca atgatgtctt gcactcaatc aaattgaaga atctgaacaa
     3541 ctacatctcc ctcttcagaa agaaaacaag gacagaaaag gagaataagg aacttgaaaa
     3601 tttggagatc aatctgagga aagagatcgc gaaagccttt aaaggcaacg aaggatacaa
     3661 aagtctgttc aagaaggata taattgagac aattttgcca gagttcctcg atgacaagga
     3721 cgagattgcg ctggtcaatt cgttcaacgg attcacaaca gcattcacag gcttctttcg
     3781 caatcgggaa aatatgttct ctgaggaggc aaagtccact tctattgcgt tcaggtgtat
     3841 caatgagaat ctcactaggt acatttccaa catggatatc tttgagaagg ttgacgcaat
     3901 ttttgacaag cacgaagttc aggagattaa ggagaagatc ctcaattccg attatgacgt
     3961 tgaggacttc ttcgaaggtg agttttttaa tttcgtgctc actcaagagg gtatcgacgt
     4021 gtataatgcg atcatcggtg ggttcgtgac tgagtccggt gaaaagatta agggattgaa
     4081 cgagtatatc aacctttaca accaaaagac gaaacagaag ctgccaaagt tcaagcctct
     4141 ttacaaacag gttctttcag accgcgagtc actctcgttc tatggggagg gctacacttc
     4201 ggatgaggaa gtcctggagg tgttcaggaa tactctcaat aagaattcgg agattttctc
     4261 ttctataaaa aaactggaaa agttgtttaa gaattttgac gaatactcta gcgccggcat
     4321 atttgtgaaa aacggcccgg ccatatcaac gataagtaaa gatatcttcg gcgaatggaa
     4381 cgtgatcaga gacaaatgga acgcggagta tgacgatatt cacctgaaga agaaggctgt
     4441 cgtaacggag aagtacgagg atgatcgcag gaaaagcttc aaaaagatcg gaagtttcag
     4501 cctggaacag ttgcaggagt atgctgacgc cgatcttagc gtcgtcgaga agttgaagga
     4561 gataatcatc caaaaggtcg acgagatata taaagtctat ggatcaagtg aaaaactgtt
     4621 cgacgccgac ttcgttttgg agaagtccct gaagaagaac gacgctgttg ttgccattat
     4681 gaaggatctg ctcgacagcg tgaagagttt cgagaactat attaaggctt ttttcgggga
     4741 ggggaaggag actaacagag atgagtcctt ctacggagac ttcgtcctcg cgtacgatat
     4801 actccttaag gtagaccaca tctacgacgc aatcagaaat tacgtgacac aaaagccgta
     4861 cagcaaggac aagttcaaac tctacttcca gaacccccag ttcatgggcg gctgggacaa
     4921 ggacaaggaa acggattaca gggctacgat cctgaggtat ggttcaaaat actacttggc
     4981 gattatggac aagaagtacg ccaagtgtct ccagaagatt gacaaagacg atgtcaatgg
     5041 caattatgag aagatcaact acaagctgct tccgggtccg aacaagatgc tcccaaaggt
     5101 tttcttcagc aagaaatgga tggcctacta taacccaagc gaggacatcc agaagattta
     5161 taagaacggt acgttcaaga agggcgacat gttcaatctt aacgactgtc acaagctgat
     5221 cgacttcttc aaagactcaa ttagccggta cccaaagtgg tctaacgcct atgacttcaa
     5281 cttttcggaa accgagaagt acaaggatat agccggattt tatagagagg tggaagagca
     5341 gggctacaag gtgtcattcg agtccgccag caagaaggaa gtggacaagc tcgtggaaga
     5401 gggtaagctc tacatgttcc agatttataa taaagacttt agcgataaga gccacgggac
     5461 acctaatctc cacacaatgt atttcaagct gctcttcgac gagaataacc acggccaaat
     5521 caggttgtca ggaggggctg aactcttcat gcggcgcgct agccttaaga aggaggagct
     5581 tgtagtccac cctgcgaata gtccaattgc gaataagaac ccggacaatc ctaaaaagac
     5641 tacaacattg agctacgacg tgtacaagga taagaggttt tccgaggatc agtacgagct
     5701 ccacatcccg attgcgatca acaagtgccc aaagaatatt ttcaagataa acacagaggt
     5761 gcgtgtactc ctgaagcatg acgacaatcc ttacgtcatt gggattgccc ggggcgagag
     5821 gaacctcctc tatattgtgg tggtggacgg gaaggggaac atagtcgaac agtactccct
     5881 taacgaaata attaacaatt tcaacggcat ccgtatcaag accgactacc attcgttgct
     5941 ggacaagaag gagaaggaga gatttgaggc gcggcaaaat tggacaagta tcgagaacat
     6001 caaggaactc aaagcaggtt atatctctca agttgtgcat aagatatgcg agctggttga
     6061 gaagtatgac gcagtgatcg ctcttgagga cctcaactcg ggctttaaga attctagagt
     6121 taaagtggag aagcaggtct atcaaaagtt cgagaagatg cttatagata agctcaacta
     6181 catggtcgat aagaaatcga acccatgtgc caccggcggc gcactcaaag gttaccaaat
     6241 aacaaacaaa ttcgagtcct tcaaatcgat gagtactcag aatgggttca tattttatat
     6301 accggcgtgg cttacgtcta agatcgaccc gtcaactggt tttgtcaacc tgttgaagac
     6361 gaaatacacg tccattgccg attcgaaaaa gttcatatct agttttgatc gtattatgta
     6421 cgtcccagag gaagatcttt tcgagtttgc tctcgactac aaaaactttt cgcggaccga
     6481 tgcggattac attaaaaaat ggaaactcta ttcgtacggc aacagaatca ggatttttcg
     6541 caaccctaag aagaataacg tctttgattg ggaggaagtt tgcttgacta gcgcgtacaa
     6601 ggagctcttt aataagtatg gcattaacta ccaacagggt gatatcagag cactgctttg
     6661 cgaacaatct gacaaggctt tctactcatc cttcatggct ttgatgagcc tgatgctcca
     6721 gatgagaaat tcaattacag gcagaaccga cgtggatttc ttgatctccc cggttaaaaa
     6781 ttctgatggc atcttttacg atagcaggaa ctatgaagcg caagagaatg cgattctgcc
     6841 aaaaaatgca gacgccaacg gtgcctataa catcgccagg aaagtcctgt gggcgatcgg
     6901 ccagttcaaa aaggccgaag acgaaaaatt ggacaaggtc aaaatcgcta tcagcaacaa
     6961 agagtggctg gagtatgctc agacatccgt aaagcataag cggccagcgg cgacgaagaa
     7021 ggcggggcag gcgaagaaga agaagggaga cggatcaggc gggtcaacaa acctcagtga
     7081 catcatagag aaggaaactg gtaagcaact ggttattcaa gagagcattc tgatgctccc
     7141 tgaggaagtc gaggaagtta taggaaacaa gcctgagagc gatatactcg tgcatacggc
     7201 gtatgacgag agcacggatg aaaatgtgat gctcttgacc agcgatgcgc cagaatataa
     7261 accctgggca ctggttattc aggactctaa cggggaaaat aaaatcaaga tgctctcagg
     7321 tggctcccca aagaagaaac gcaaggttta gtggcggccg ccgacgtccg atcgttcaaa
     7381 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     7441 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     7501 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     7561 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     7621 tcgggaattg atcccccctc gacagcttcc ggaaagggcg aattcgcaac tttgtataca
     7681 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     7741 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg ccatccagct
     7801 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     7861 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
//
