LOCUS       Bxb1(Int)-GS(12)-deCFP_-_Victoria's_Archive_(pVHab1_Bxb1)        4524 bp    DNA     circular UNA 02-DEC-2022
DEFINITION  Ptet-BCD2-Bxb1-GS(12)-deCFP.
ACCESSION   urn.local.maksmac.l-fmbyoq7
VERSION     urn.local.maksmac.l-fmbyoq7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..13
                     /created_by="User"
                     /label="prefix BBa"
     promoter        14..67
                     /created_by="User"
                     /label="pTetO"
     misc            68..155
                     /created_by="User"
                     /modified_by="User"
                     /label="BCD2_apFAB682"
     misc_feature    153..155
                     /created_by="User"
                     /label="atg"
     misc_feature    155^156
                     /Original_Bases="GTG"
                     /label="GTG"
                     /note="Geneious type: Editing History Deletion"
     gene            <156..>1652
                     /created_by="User"
                     /label="Bxb1 Integrase"
     misc_feature    complement(388..407)
                     /created_by="User"
                     /label="pVHed05_seq_REV"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1361..1376
                     /Sequence="AGAAGCACTGGATGCC"
                     /Hairpin_Tm="57.9"
                     /Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /%GC="56.3"
                     /created_by="primer3"
                     /label="Bxb1_seq_F"
     misc_feature    complement(1635..1689)
                     /created_by="User"
                     /Sequence="TGCTGCCGCTGCCACTACCGGATCCACTTCCGCTTCCCGACATCCCG
                     GTGTGTAG"
                     /modified_by="User"
                     /label="pC1:bb_R(65C)"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1635..1652)
                     /Sequence="CGACATCCCGGTGTGTAG"
                     /Hairpin_Tm="None"
                     /Tm="57.2"
                     /Self_Dimer_Tm="1.8"
                     /%GC="61.1"
                     /created_by="primer3"
                     /label="pC1:bb_R"
                     /note="Geneious type: primer_bind_reverse"
     linker          1653..1688
                     /created_by="User"
                     /modified_by="User"
                     /label="GS linker"
     misc_feature    1652^1653
                     /Original_Bases="TAA"
                     /label="TAA"
                     /note="Geneious type: Editing History Deletion"
     primer_bind     1670..1729
                     /created_by="User"
                     /Sequence="CGGTAGTGGCAGCGGCAGCATGCATCATCACCACCACCATGAGCTTT
                     TCACTGGCGTTGT"
                     /modified_by="User"
                     /label="deCFP_F (67)"
     gene            1689..2384
                     /created_by="User"
                     /Color="#0055cc"
                     /modified_by="User"
                     /label="deCFP "
     misc_feature    1689..2420
                     /created_by="User"
                     /modified_by="User"
                     /label="From Vincent library plasmid"
     misc_feature    1692..1709
                     /created_by="User"
                     /label="6x His tag"
     primer_bind     1710..1729
                     /Sequence="GAGCTTTTCACTGGCGTTGT"
                     /Hairpin_Tm="None"
                     /Tm="59.3"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="deCFP F"
     misc_feature    complement(1823..1840)
                     /Sequence="GTGCAGATGAACTTCAGG"
                     /Hairpin_Tm="None"
                     /Tm="53.0"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="deCFP_seq_R"
                     /note="Geneious type: primer_bind_reverse"
     ORF             <2385..>2420
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /label="ORF 1 (frame 2)"
     ORF             complement(<2385..>2420)
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /label="ORF 49 (frame 1)"
     ORF             complement(<2385..>2420)
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /label="ORF 48 (frame 3)"
     ORF             complement(<2385..2393)
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /label="ORF 50 (frame 2)"
     terminator      2391..2420
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="#7d5aa5"
                     /modified_by="User"
                     /label="T500 terminator"
     misc_feature    complement(2401..2440)
                     /created_by="User"
                     /Sequence="GCAGCGGCCGCTACTAGTATACAGAAAAGCCCGCCTTTCG"
                     /modified_by="User"
                     /label="deCFP_R(67C)"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2401..2420)
                     /Sequence="ACAGAAAAGCCCGCCTTTCG"
                     /Hairpin_Tm="42.8"
                     /Tm="61.5"
                     /Self_Dimer_Tm="4.0"
                     /%GC="55.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="deCFP_R binding"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2421..2441
                     /created_by="User"
                     /modified_by="User"
                     /label="Suffix BBa"
     primer_bind     2421..2438
                     /Sequence="ATACTAGTAGCGGCCGCT"
                     /Hairpin_Tm="53.3"
                     /Tm="57.5"
                     /Self_Dimer_Tm="49.3"
                     /%GC="55.6"
                     /modified_by="User"
                     /label="pC1:bb_F"
     misc_feature    complement(2465..2482)
                     /Sequence="AAGGGCAGGGTGGTGACA"
                     /Hairpin_Tm="38.7"
                     /Tm="60.8"
                     /Self_Dimer_Tm="None"
                     /%GC="61.1"
                     /modified_by="User"
                     /label="pC_bb_seq_R"
                     /note="Geneious type: primer_bind_reverse"
     ori             complement(2730..3412)
                     /created_by="User"
                     /modified_by="User"
                     /label="ColE1"
     resistance      3537..4198
                     /created_by="User"
                     /label="Chloramphenicol R"
     primer_bind     4461..4480
                     /created_by="User"
                     /label="DRC_seq_FWD"
ORIGIN      
        1 gaattttcta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
       61 tgagcacggg cccaagttca cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg
      121 aaacatctta atcatgctaa ggaggttttc taatgagagc cctggtagtc atccgcctgt
      181 cccgcgtcac cgatgctacg acttcaccgg agcgtcagct ggagtcttgc cagcagctct
      241 gcgcccagcg cggctgggac gtcgtcgggg tagcggagga tctggacgtc tccggggcgg
      301 tcgatccgtt cgaccggaag cgcagaccga acctggcccg gtggctagcg ttcgaggagc
      361 aaccgtttga cgtgatcgtg gcgtaccggg tagatcggtt gacccgatcg atccggcatc
      421 ttcagcagct ggtccactgg gccgaggacc acaagaagct ggtcgtctcc gcgaccgaag
      481 cgcacttcga tacgacgacg ccgtttgcgg cggtcgtcat cgcgcttatg ggaacggtgg
      541 cgcagatgga attagaagcg atcaaagagc ggaaccgttc ggctgcgcat ttcaatatcc
      601 gcgccgggaa ataccgagga tccctgccgc cgtggggata cctgcctacg cgcgtggacg
      661 gggagtggcg gctggtgccg gaccctgtgc agcgagagcg catcctcgag gtgtatcacc
      721 gcgtcgtcga caaccacgag ccgctgcatc tggtggccca cgacctgaac cggcgtggtg
      781 tcctgtcgcc gaaggactac ttcgcgcagc tgcaaggccg cgagccgcag ggccgggagt
      841 ggtcggctac cgcgctgaag cgatcgatga tctccgaggc gatgctcggg tacgcgactc
      901 tgaacggtaa gaccgtccga gacgacgacg gagccccgct ggtgcgggct gagccgatcc
      961 tgacccgtga gcagctggag gcgctgcgcg ccgagctcgt gaagacctcc cgggcgaagc
     1021 ccgcggtgtc taccccgtcg ctgctgctgc gggtgttgtt ctgcgcggtg tgcggggagc
     1081 ccgcgtacaa gttcgccggg ggaggacgta agcacccgcg ctaccgctgc cgctcgatgg
     1141 ggttcccgaa gcactgcggg aacggcacgg tggcgatggc cgagtgggac gcgttctgcg
     1201 aggagcaggt actggatctg ctcggggacg cggagcgtct ggagaaagtc tgggtagcgg
     1261 gctcggactc cgcggtcgaa ctcgcggagg tgaacgcgga gctggtggac ctgacgtcgc
     1321 tgatcggctc cccggcctac cgggcgggct ctccgcagcg agaagcactg gatgcccgta
     1381 ttgcggcgct ggccgcgcgg caagaggagc tggagggcct ggaggctcgc ccgtctggct
     1441 gggagtggcg cgagaccggg cagcggttcg gggactggtg gcgggagcag gacaccgcgg
     1501 caaagaacac ctggcttcgg tcgatgaacg ttcggctgac gttcgacgtc cgcggcgggc
     1561 tgactcgcac gatcgacttc ggggatcttc aggagtacga gcagcatctc aggctcggca
     1621 gcgtggtcga acggctacac accgggatgt cgggaagcgg aagtggatcc ggtagtggca
     1681 gcggcagcat gcatcatcac caccaccatg agcttttcac tggcgttgtt cccatcctgg
     1741 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1801 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1861 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg
     1921 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1981 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2041 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2101 tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca
     2161 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg
     2221 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2281 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2341 atcacatggt cctgctggag ttcgtgaccg ccgccgggat ctaactcgag caaagcccgc
     2401 cgaaaggcgg gcttttctgt atactagtag cggccgctgc agtccggcaa aaaaacgggc
     2461 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag
     2521 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2581 cactcaaagg cggtaatcct agggcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2941 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3181 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgacttgtgc
     3421 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     3481 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     3541 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     3601 tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     3661 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     3721 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     3781 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     3841 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     3901 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     3961 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     4021 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     4081 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     4141 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     4201 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     4261 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag
     4321 atatcgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4381 cgaggccctt tcgtcttcac ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     4441 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     4501 agctttcgct aaggatgatt tctg
//
