LOCUS       New_DNA                 5582 bp ds-DNA     circular     15-OCT-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3608..4142
                     /locus_tag="Rabit globbin poly A"
                     /label="Rabit globbin poly A"
                     /ApEinfo_label="Rabit globbin poly A"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3024..3596
                     /locus_tag="p65(aa361-551)"
                     /label="p65(aa361-551)"
                     /ApEinfo_label="p65(aa361-551)"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4893..5575)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(4186..4208)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(1104..1178,1180..1500,1502..1878,1880..1922,
                     1924..2307)
                     /locus_tag="pUb"
                     /label="pUb"
                     /ApEinfo_label="pUb"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#8080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2325..2330
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(91..750)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(990..1018)
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4333..4669
                     /locus_tag="sv40 ori"
                     /label="sv40 ori"
                     /ApEinfo_label="sv40 ori"
                     /ApEinfo_fwdcolor="#004080"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2331..2333
                     /locus_tag="Start"
                     /label="Start"
                     /ApEinfo_label="Start"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2457..2474
                     /locus_tag="GS linker"
                     /label="GS linker"
                     /ApEinfo_label="GS linker"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     2334..2450
                     /gene="3xFlag-NLS"
                     /SECDrawAs="Label"
                     /locus_tag="3xFlag-NLS"
                     /label="3xFlag-NLS"
                     /ApEinfo_label="3xFlag-NLS"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3015..3023
                     /locus_tag="SbfI-HF (for ZF swap)"
                     /label="SbfI-HF (for ZF swap)"
                     /ApEinfo_label="SbfI-HF (for ZF swap)"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2481..2486
                     /locus_tag="NheI-HF (for ZF swap)"
                     /label="NheI-HF (for ZF swap)"
                     /ApEinfo_label="NheI-HF (for ZF swap)"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3597..3599
                     /locus_tag="Stop"
                     /label="Stop"
                     /ApEinfo_label="Stop"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#808000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2487..3014
                     /locus_tag="ZF4-8"
                     /label="ZF4-8"
                     /ApEinfo_label="ZF4-8"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
       61 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
      121 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
      181 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC
      241 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
      301 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
      361 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
      421 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
      481 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
      541 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
      601 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
      661 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
      721 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
      781 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
      841 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
      901 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
      961 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     1021 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGG
     1081 GTCGACATTG ATTATTGACT AGTgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     1141 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     1201 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     1261 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     1321 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     1381 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     1441 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     1501 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     1561 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     1621 ggggttgggg ggagcgcaca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     1681 taaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     1741 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     1801 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     1861 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     1921 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     1981 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     2041 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     2101 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     2161 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     2221 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     2281 tggccgtttt tggctttttt gttagacACG CGTttaatta aGCCgccacc atggactaca
     2341 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga
     2401 tggcccccaa gaaaaagagg aaggtgggca ttcacggggt gccgggtgga ctcgagggag
     2461 gcggtggaag cggcggtacc gctagcTCTA GACCAGGCGA ACGACCGTTT CAATGCCGGA
     2521 TATGTATGAG GAACTTCTCC CAGCAAACCA ATCTGACGAG ACACACCCGA ACACATACAG
     2581 GAGAAAAGCC ATTCCAATGT CGAATTTGTA TGCGCAATTT TTCACAATCC ACAAGCCTTC
     2641 AACGACACCT CCGCACACAT ACTGGTTCAC AGAAGCCCTT TCAGTGCAGG ATTTGTATGC
     2701 GCAACTTTAG CGTGCGGCAT AACCTTACGC GGCACACTAG AACGCATACA GGTGAGAAGC
     2761 CTTTCCAGTG TCGCATCTGT ATGCGGAACT TCAGCGACCA CAGCAACCTC AGTCGGCATT
     2821 TGCGAACTCA CACCGGTTCT CAAAAACCTT TTCAGTGCCG AATTTGTATG CGCAACTTCA
     2881 GCGAAAAGCA AAATCTCGCT CGCCACACGC GCACGCACAC CGGGGAAAAA CCGTTTCAAT
     2941 GTCGAATCTG CATGCGCAAT TTTAGCGACC CAAGCAACTT GCGGCGACAT CTGCGCACAC
     3001 ACTTGCGGGG ATCCaCCTGC AGGGATGAGT TTCCCACCAT GGTGTTTCCT TCTGGGCAGA
     3061 TCAGCCAGGC CTCGGCCTTG GCCCCGGCCC CTCCCCAAGT CCTGCCCCAG GCTCCAGCCC
     3121 CTGCCCCTGC TCCAGCCATG GTATCAGCTC TGGCCCAGGC CCCAGCCCCT GTCCCAGTCC
     3181 TAGCCCCAGG CCCTCCTCAG GCTGTGGCCC CACCTGCCCC CAAGCCCACC CAGGCTGGGG
     3241 AAGGAACGCT GTCAGAGGCC CTGCTGCAGC TGCAGTTTGA TGATGAAGAC CTGGGGGCCT
     3301 TGCTTGGCAA CAGCACAGAC CCAGCTGTGT TCACAGACCT GGCATCCGTC GACAACTCCG
     3361 AGTTTCAGCA GCTGCTGAAC CAGGGCATAC CTGTGGCCCC CCACACAACT GAGCCCATGC
     3421 TGATGGAGTA CCCTGAGGCT ATAACTCGCC TAGTGACAGG GGCCCAGAGG CCCCCCGACC
     3481 CAGCTCCTGC TCCACTGGGG GCCCCGGGGC TCCCCAATGG CCTCCTTTCA GGAGATGAAG
     3541 ACTTCTCCTC CATTGCGGAC ATGGACTTCT CAGCCCTGCT GAGTCAGATC AGCTCCTAAG
     3601 CGGCCGCACT CCTCAGGTGC AGGCTGCCTA TCAGAAGGTG GTGGCTGGTG TGGCCAATGC
     3661 CCTGGCTCAC AAATACCACT GAGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT
     3721 GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT
     3781 GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTAAA
     3841 ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT GCCATATGCT GGCTGCCATG
     3901 AACAAAGGTG GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCT
     3961 TATTCCATAG AAAAGCCTTG ACTTGAGGTT AGATTTTTTT TATATTTTGT TTTGTGTTAT
     4021 TTTTTTCTTT AACATCCCTA AAATTTTCCT TACATGTTTT ACTAGCCAGA TTTTTCCTCC
     4081 TCTCCTGACT ACTCCCAGTC ATAGCTGTCC CTCTTCTCTT ATGAAGATCC CTCGACCTGC
     4141 AGCCCAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
     4201 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
     4261 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
     4321 TCGTGCCAGC GGATCCGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC
     4381 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT
     4441 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG
     4501 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTAAC TTGTTTATTG CAGCTTATAA
     4561 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA
     4621 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT
     4681 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC
     4741 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
     4801 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
     4861 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
     4921 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     4981 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     5041 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     5101 TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     5161 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     5221 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
     5281 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
     5341 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
     5401 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
     5461 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
     5521 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     5581 TC     
//
