LOCUS       Exported               17719 bp ds-DNA     circular SYN 24-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRMD1 - pAct_evoAPOBEC1_Cas9D10A _1xUGI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17719)
  AUTHORS   Jan Winter
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 3, 2023 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17719
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_signal     236
                     /label=MCS [Split]
     misc_feature    242..246
                     /label=Kozak
     misc_feature    247..1083
                     /label=evoAPOBEC1
     CDS             247..282
                     /codon_start=1
                     /label=Feature 33
                     /translation="MKRTADGSEFES"
     CDS             283..303
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1084..5187
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5218..5466
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5509..5529
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_signal     5534..5550
                     /label=MCS [Split]
     misc_signal     5551..6250
                     /label=SV40 poly A
     intron          5628..5693
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
     CDS             5823..5843
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6115..6249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_signal     6257..6541
                     /label=att B
     protein_bind    6278..6347
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(6566..6584)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6605..6621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6629..6645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6653..6683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6698..6719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7007..7595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7766..8626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_signal     7769..8626
                     /note="Amp. res."
     promoter        complement(8627..8731)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8757..9212)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     9354..9370
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9377..9395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            9421..13556
                     /label=white
     CDS             join(9899..9970,10372..10645,10720..11374,11436..11751,
                     11972..12103,12174..12788)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This ia a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(13563..13596)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_signal     13563..13596
                     /label=loxP
     misc_signal     13603..13614
                     /label=UAS-hsp70
     promoter        13744..16199
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5c promoter"
ORIGIN
        1 caagattaaa attttgtact aaaaaaacgt acattgcatt gagtgatttt taattgtaca
       61 cgaaaaacaa gttagtttgt tatgacaatt gtactttggt agaccagcgc agtccaagga
      121 aaccacgcaa attctcagtt ttttttttgc catttctaca ttaccaaata aggtaaccaa
      181 aaactaatgg gaaatccgca ttctttccat tgcagcttac aggatcgatc cccgggaatt
      241 cccaccatga aacggacagc cgacggaagc gagttcgagt caccaaagaa gaagcggaaa
      301 gtcagttcaa agactgggcc tgtcgccgtc gatccaaccc tgcgccgccg gattgaacct
      361 cacgagtttg aagtgttctt tgacccccgg gagctgagaa aggagacatg cctgctgtac
      421 gagatcaact ggggaggcag gcactccatc tggaggcaca cctctcagaa cacaaataag
      481 cacgtggagg tgaacttcat cgagaagttt accacagagc ggtacttctg ccccaatacc
      541 agatgtagca tcacatggtt tctgagctgg tccccttgcg gagagtgtag cagggccatc
      601 accgagttcc tgtccagata tccaaatgtg acactgttta tctacatcgc caggctgtat
      661 cacctggcaa acccaaggaa taggcagggc ctgcgcgatc tgatcagctc cggcgtgacc
      721 atccagatca tgacagagca ggagtccggc tactgctggc acaacttcgt gaattattct
      781 cctagcaacg agtcccactg gcctaggtac ccacacctgt gggtgcgcct gtacgtgctg
      841 gagctgtatt gcatcatcct gggcctgccc ccttgtctga atatcctgcg gagaaagcag
      901 agccagctga cctcctttac aatcgccctg cagtcttgtc actatcagag gctgccaccc
      961 cacatcctgt gggccacagg cctgaagtct ggcggatcta gcggaggatc ctctggcagc
     1021 gagacaccag gaacaagcga gtcagcaaca ccagagagca gtggcggcag cagcggcggc
     1081 agcatggaca agaagtactc cattgggctc gccatcggca caaacagcgt cggctgggcc
     1141 gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat
     1201 cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc
     1261 gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc
     1321 tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat
     1381 aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt
     1441 ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg
     1501 aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg
     1561 catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc
     1621 gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac
     1681 ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc
     1741 cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt
     1801 aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc
     1861 gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg
     1921 gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc
     1981 attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct
     2041 agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc
     2101 agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac
     2161 gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc
     2221 ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg
     2281 cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg
     2341 cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag
     2401 attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat
     2461 tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag
     2521 gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat
     2581 aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca
     2641 gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc
     2701 ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt
     2761 accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa
     2821 atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa
     2881 atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt
     2941 gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac
     3001 gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg
     3061 gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc
     3121 ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat
     3181 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt
     3241 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag
     3301 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc
     3361 gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa
     3421 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac
     3481 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc
     3541 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat
     3601 catatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga
     3661 tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg
     3721 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat
     3781 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg
     3841 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg
     3901 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag
     3961 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac
     4021 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa
     4081 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg
     4141 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac
     4201 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag
     4261 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat
     4321 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa
     4381 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg
     4441 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc
     4501 gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc
     4561 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc
     4621 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc
     4681 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag
     4741 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc
     4801 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg
     4861 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga
     4921 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat
     4981 aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc
     5041 gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca
     5101 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga
     5161 atcgacctct ctcagctcgg tggagacagc ggcggttccg gtggatccgg cggaagcacg
     5221 aacctgagtg atataattga aaaggaaacc ggtaaacagc tcgttatcca ggagtcgatc
     5281 ctcatgctgc ccgaggaagt cgaggaagtc atcggtaaca aaccggaatc ggatattctg
     5341 gtacatacgg cctatgatga atccaccgat gagaatgtaa tgttgttgac atcggatgca
     5401 ccggagtata agccttgggc gctcgtcatc caggactcca atggcgagaa caagattaag
     5461 atgctctccg gaggatccaa acgaacagct gacggtagtg aattcgaacc aaaaaaaaag
     5521 cgcaaggtat aactcgaggg tacctctaga ggatctttgt gaaggaacct tacttctgtg
     5581 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     5641 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
     5701 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
     5761 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
     5821 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
     5881 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
     5941 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct
     6001 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     6061 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     6121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6241 gtctggatcc actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
     6301 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
     6361 tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct
     6421 cgccctcgaa accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg
     6481 cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga
     6541 cactagttct agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     6601 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6661 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6721 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7141 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7201 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7261 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7321 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7381 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7441 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7501 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7561 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7621 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7681 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     8761 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     8821 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     8881 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     8941 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     9001 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     9061 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     9121 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     9181 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     9241 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     9301 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     9361 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacgt accgggcccc
     9421 tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt
     9481 ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat
     9541 ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac
     9601 tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc
     9661 gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct
     9721 tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta
     9781 aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga
     9841 ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat
     9901 gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca
     9961 tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact
    10021 gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc
    10081 gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata
    10141 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta
    10201 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa
    10261 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt
    10321 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc
    10381 ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg
    10441 ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac
    10501 ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga
    10561 tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg
    10621 cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga
    10681 aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg
    10741 gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc
    10801 ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg
    10861 gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc
    10921 tctttatcgg ctccctaacg gccagggaac acctgatttt ccaggccatg gtgcggatgc
    10981 cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt
    11041 cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg
    11101 gcggagaaag gaagcgtctg gcattcgcct ccgaggcact aaccgatccg ccgcttctga
    11161 tctgcgatga gcccacctcc ggactggact catttaccgc ccacagcgtc gtccaggtgc
    11221 tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg
    11281 agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg
    11341 gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt
    11401 atctagcatt acattacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac
    11461 tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag
    11521 tcccgtgatc ggatcgccaa gatatgcgac aattttgcta ttagcaaagt agcccgggat
    11581 atggagcagt tgttggccac caaaaatttg gagaagccac tggagcagcc ggagaatggg
    11641 tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg
    11701 tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt
    11761 tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta
    11821 gttagaattg cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata
    11881 gttttgcaga aaataaataa atttcattta actcgcgaac atgttgaaga tatgaatatt
    11941 aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg gcctcatctt
    12001 tttgggccaa caactcacgc aagtgggcgt gatgaatatc aacggagcca tcttcctctt
    12061 cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc ttgtttagaa
    12121 tacatttgca tattaataat ttactaactt tctaatgaat cgattcgatt taggtgttca
    12181 cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc tgtgacacat
    12241 actttctggg caaaacgatt gccgaattac cgctttttct cacagtgcca ctggtcttca
    12301 cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc ttcaactgcc
    12361 tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta atatcctgcg
    12421 ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata ccattcctgc
    12481 tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt
    12541 acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg
    12601 agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca
    12661 tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca
    12721 ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca
    12781 aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc attattacca
    12841 tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa
    12901 aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca caagtagact
    12961 ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt
    13021 ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc
    13081 cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca
    13141 cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt
    13201 ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta
    13261 gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat atttccctca
    13321 cagctatatt tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt
    13381 cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta
    13441 gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa
    13501 ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg aactaggcta
    13561 gcataacttc gtataatgta tgctatacga agttatgcta gcggatccaa gctttgcggc
    13621 cgcaattcta tattctaaaa acacaaatga tacttctaaa aaaaaatcat gaatggcatc
    13681 aactctgaat caaatctttg cagatgcacc tacttctcat ttccactgtc acatcatttt
    13741 tccagatctc gctgcctgtt atgtggccca caaaccaaga cacgttttat ggccattaaa
    13801 gctggctgat cgtcgccaaa caccaaatac ataatgaata tgtacacatt cgagaaagaa
    13861 gcgatcaaag aagcgtcttc gggcggagta ggagaatgcg gaggagaagg agaacgagct
    13921 gatctagtat ctctccacaa tccaatgcca actgaccaac tggccatatt cggagcaatt
    13981 tgaagccaat ttccatcgcc tggcgatcgc tccattcttg gctatatgtt tttcaccgtt
    14041 acccggggcc attttcaaag actcgtcggc aagataagat tgtgtcactc gctgtctctc
    14101 ttcatttgtc gaagaatgct gaggaatttc gcgatgacgt cggcgagtat tttgaagaat
    14161 gagaataatt tgtatttata cgaaaatcag ttagtggaat tttctacaaa aacatgttat
    14221 ctatagataa ttttgttgca aaatatgttg actatgacaa agattgtatg tatatacctt
    14281 taatgtattc tcattttctt atgtatttat aatggcaatg atgatactga tgatatttta
    14341 agatgatgcc agaccacagg ctgatttctg cgtcttttgc cgaacgcagt gcatgtgcgg
    14401 ttgttgtttt ttggaatagt ttcaattttc ggactgtccg ctttgatttc agtttcttgg
    14461 cttattcaaa aagcaaagta aagccaaaaa agcgagatgg caataccaaa tgcggcaaaa
    14521 cggtagtgga aggaaagggg tgcggggcag cggaaggaag ggtggggcgg ggcgtggcgg
    14581 ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc atgtgtgcgt
    14641 gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat ctcgctctct
    14701 ctagcgccat ctcgtacgca tgctcaacgc accgcatgtt gccgtgtcct ttatgcgtca
    14761 ttttggctcg aaataggcaa ttatttaaac aaagattagt caacgaaaac gctaaaataa
    14821 ataagtctac aatatggtta cttattgcca tgtgtgtgca gccaacgata gcaacaaaag
    14881 caacaacaca gtggctttcc ctctttcact ttttgtttgc aagcgcgtgc gagcaagacg
    14941 gcacgaccgg caaacgcaat tacgctgaca aagagcagac gaagttttgg ccgaaaaaca
    15001 tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc gatatttaat attgtttatg
    15061 aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa caaattattt gaaagagaat
    15121 taggaatcgg acagcttatc gttacgggct aacagcacac cgagacgaaa tagcttacct
    15181 gacgtcacag cctctggaag aactgccgcc aagcagacga tgcagaggac gacacataga
    15241 gtagcggagt aggccagcgt agtacgcatg tgcttgtgtg tgaggcgtct ctctcttcgt
    15301 ctcctgtttg cgcaaacgca tagactgcac tgagaaaatc gattacctat tttttatgaa
    15361 tgaatatttg cactattact attcaaaact attaagatag caatcacatt caatagccaa
    15421 atactatacc acctgagcga tgcaacgaaa tgatcaattt gagcaaaaat gctgcatatt
    15481 taggacggca tcattataga aatgcttctt gctgtgtact tttctctcgt ctggcagctg
    15541 tttcgccgtt attgttaaaa ccggcttaag ttaggtgtgt tttctacgac tagtgatgcc
    15601 cctactagaa gatgtgtgtt gcacaaatgt ccctgaataa ccaatttgaa gtgcagatag
    15661 cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat cgctggacat
    15721 tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac aatgagtagt
    15781 tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac cagctgaagt
    15841 atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga cccatggaag
    15901 tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt tgcggaggaa
    15961 attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg gtaaaagttt
    16021 tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc taagcgggct
    16081 ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc ttgtgctgtg
    16141 tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa tccagagaca
    16201 ccaaaccgaa agacttaatt tatatttatt taattaattt taataaaaca caccaaatgt
    16261 aagtagcttt ccccttccca acaacaaaac accatcgaac cactcccacc aagaaaaagc
    16321 aataatcgag aaaagccgcg gaaaatgtgt gatttttttt gtaaacaaaa cattttttta
    16381 tgtgccagtg ctgaaagtga tcaaaaaata ctagccacga gctaaagagt tattgtattg
    16441 accaaaactc caaaaatacc caagtttggc cctaaattgt caatcaaaat accaataggt
    16501 cgaaagacat caaaattaac aaaaccaggg tttcaaatac cataactcaa gaatcaggat
    16561 tacaactgca gatttcagga tatatacata caaattatac gaaattataa aaaccaaagc
    16621 aattcaatag ccccaactca aatgttagga tctaatatag tgtttaaagc caagctcgct
    16681 gatgtgggcg tgtcacgatt tcacccaaag atatgccaaa ttacgaattg caaatcaatt
    16741 cgccaacact ttcttttttt cccacgccct aaaacaccag atcatccata aatgtacata
    16801 catacagtat atgcatatta taatctgtaa aactagatca ggttcttgaa aatagtgacg
    16861 taggcagccg ttttggctga agcagaaatt tttgccggtt tttcaaagtt gtagttgcaa
    16921 aaatggagaa aaccttcgag cattcgttca tatacacaca ctcacgcgca aaataacgag
    16981 agagagtgta tgtgtgtgtg agagagcgaa agccagacga cggtttgctt ttcgcctcga
    17041 aacatgacca tatatggtca caaaacttgg ccgccgcaat tcaacacacc agcgctctcc
    17101 ttcgcaccca tagcgaccat ggcgcggagc gagcgagatg gcgagagcga gcgacgccta
    17161 tggcgacgtc gacgcaggca gcgattgaaa aacgcagtta actggcattc aacattcacc
    17221 agccactttc agtcggttta ttccagtcat tcctttcaaa ccgtgcggtc gcttagctca
    17281 gcctcgccac ttgcgtttac agtagttttc acgccttgaa tttgttaaat cgaacaaaaa
    17341 ggtaaagttt aactagcttt gaaaagtttc gtggctctta attgttaaat tttctagagt
    17401 gcgtttagtg tttttttttt tttttatttt gtaatgttaa tttcgggttc caattcgagt
    17461 tttaggcagc cgccatttta agggcgcata cacacaggca actgtgctct ctttgcggct
    17521 ttcttttgca ccggcattcg ttaagtgtcg tctagaagct tctcccctcc cttttcggca
    17581 tattcgtatt gtggttttaa tttttcgggg cggggccttc tattttgtaa ctgttctttt
    17641 aatttcttat tacaattcga tcgcaagtga aaatcagttt tcaatcggaa aagtattttt
    17701 ttatgaaatt ttttttgtc
//
