LOCUS       pBbA5K-acrAB-sfgfp      8769 bp ds-DNA     circular     07-DEC-2022
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pBba5k-acrAB-sfgfp.gb
FEATURES             Location/Qualifiers
     misc_feature    1..76
                     /label="lacUV5 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     RBS             96..115
                     /label="RBS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            116..1309
                     /label="acrA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1332..4478
                     /label="acrB"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     RBS             4479..4506
                     /label="RBS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4507..5226
                     /label="sfgfp"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5227..5244
                     /label="his tag"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    5272..5400
                     /label="dbl term"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(5407..6118)
                     /label="p15a"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     terminator      6209..6314
                     /label="TO"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(6340..7134)
                     /label="Kan/neoR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(7319..8401)
                     /label="lacI"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 ATCGTTTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATAAT GTGTGGAATT
       61 GTGAGCGGAT AACAATTTCA GAATTCAAAA GATCTTTTAA GAAGGAGATA TACATATGAA
      121 CAAAAACAGA GGGTTTACGC CTCTGGCGGT CGTTCTGATG CTCTCAGGCA GCTTAGCCCT
      181 AACAGGATGT GACGACAAAC AGGCCCAACA AGGTGGCCAG CAGATGCCCG CCGTTGGCGT
      241 AGTAACAGTC AAAACTGAAC CTCTGCAGAT CACAACCGAG CTTCCGGGTC GCACCAGTGC
      301 CTACCGGATC GCAGAAGTTC GTCCTCAAGT TAGCGGGATT ATCCTGAAGC GTAATTTCAA
      361 AGAAGGTAGC GACATCGAAG CAGGTGTCTC TCTCTATCAG ATTGATCCTG CGACCTATCA
      421 GGCGACATAC GACAGTGCGA AAGGTGATCT GGCGAAAGCC CAGGCTGCAG CCAATATCGC
      481 GCAATTGACG GTGAATCGTT ATCAGAAACT GCTCGGTACT CAGTACATCA GTAAGCAAGA
      541 GTACGATCAG GCTCTGGCTG ATGCGCAACA GGCGAATGCT GCGGTAACTG CGGCGAAAGC
      601 TGCCGTTGAA ACTGCGCGGA TCAATCTGGC TTACACCAAA GTCACCTCTC CGATTAGCGG
      661 TCGCATTGGT AAGTCGAACG TGACGGAAGG CGCATTGGTA CAGAACGGTC AGGCGACTGC
      721 GCTGGCAACC GTGCAGCAAC TTGATCCGAT CTACGTTGAT GTGACCCAGT CCAGCAACGA
      781 CTTCCTGCGC CTGAAACAGG AACTGGCGAA TGGCACGCTG AAACAAGAGA ACGGCAAAGC
      841 CAAAGTGTCA CTGATCACCA GTGACGGCAT TAAGTTCCCG CAGGACGGTA CGCTGGAATT
      901 CTCTGACGTT ACCGTTGATC AGACCACTGG GTCTATCACC CTACGCGCTA TCTTCCCGAA
      961 CCCGGATCAC ACTCTGCTGC CGGGTATGTT CGTGCGCGCA CGTCTGGAAG AAGGGCTTAA
     1021 TCCAAACGCT ATTTTAGTCC CGCAACAGGG CGTAACCCGT ACGCCGCGTG GCGATGCCAC
     1081 CGTACTGGTA GTTGGCGCGG ATGACAAAGT GGAAACCCGT CCGATCGTTG CAAGCCAGGC
     1141 TATTGGCGAT AAGTGGCTGG TGACAGAAGG TCTGAAAGCA GGCGATCGCG TAGTAATAAG
     1201 TGGGCTGCAG AAAGTGCGTC CTGGTGTCCA GGTAAAAGCA CAAGAAGTTA CCGCTGATAA
     1261 TAACCAGCAA GCCGCAAGCG GTGCTCAGCC TGAACAGTCC AAGTCTTAAC TTAAACAGGA
     1321 GCCGTTAAGA CATGCCTAAT TTCTTTATCG ATCGCCCGAT TTTTGCGTGG GTGATCGCCA
     1381 TTATCATCAT GTTGGCAGGG GGGCTGGCGA TCCTCAAACT GCCGGTGGCG CAATATCCTA
     1441 CGATTGCACC GCCGGCAGTA ACGATCTCCG CCTCCTACCC CGGCGCTGAT GCGAAAACAG
     1501 TGCAGGACAC GGTGACACAG GTTATCGAAC AGAATATGAA CGGTATCGAT AACCTGATGT
     1561 ACATGTCCTC TAACAGTGAC TCCACGGGTA CCGTGCAGAT CACCCTGACC TTTGAGTCTG
     1621 GTACTGATGC GGATATCGCG CAGGTTCAGG TGCAGAACAA ACTGCAGCTG GCGATGCCGT
     1681 TGCTGCCGCA AGAAGTTCAG CAGCAAGGGG TGAGCGTTGA GAAATCATCC AGCAGCTTCC
     1741 TGATGGTTGT CGGCGTTATC AACACCGATG GCACCATGAC GCAGGAGGAT ATCTCCGACT
     1801 ACGTGGCGGC GAATATGAAA GATGCCATCA GCCGTACGTC GGGCGTGGGT GATGTTCAGT
     1861 TGTTCGGTTC ACAGTACGCG ATGCGTATCT GGATGAACCC GAATGAGCTG AACAAATTCC
     1921 AGCTAACGCC GGTTGATGTC ATTACCGCCA TCAAAGCGCA GAACGCCCAG GTTGCGGCGG
     1981 GTCAGCTCGG TGGTACGCCG CCGGTGAAAG GCCAACAGCT TAACGCCTCT ATTATTGCTC
     2041 AGACGCGTCT GACCTCTACT GAAGAGTTCG GCAAAATCCT GCTGAAAGTG AATCAGGATG
     2101 GTTCCCGCGT GCTGCTGCGT GACGTCGCGA AGATTGAGCT GGGTGGTGAG AACTACGACA
     2161 TCATCGCAGA GTTTAACGGC CAACCGGCTT CCGGTCTGGG GATCAAGCTG GCGACCGGTG
     2221 CAAACGCGCT GGATACCGCT GCGGCAATCC GTGCTGAACT GGCGAAGATG GAACCGTTCT
     2281 TCCCGTCGGG TCTGAAAATT GTTTACCCAT ACGACACCAC GCCGTTCGTG AAAATCTCTA
     2341 TTCACGAAGT GGTTAAAACG CTGGTCGAAG CGATCATCCT CGTGTTCCTG GTTATGTATC
     2401 TGTTCCTGCA GAACTTCCGC GCGACGTTGA TTCCGACCAT TGCCGTACCG GTGGTATTGC
     2461 TCGGGACCTT TGCCGTCCTT GCCGCCTTTG GCTTCTCGAT AAACACGCTA ACAATGTTCG
     2521 GGATGGTGCT CGCCATCGGC CTGTTGGTGG ATGACGCCAT CGTTGTGGTA GAAAACGTTG
     2581 AGCGTGTTAT GGCGGAAGAA GGTTTGCCGC CAAAAGAAGC TACCCGTAAG TCGATGGGGC
     2641 AGATTCAGGG CGCTCTGGTC GGTATCGCGA TGGTACTGTC GGCGGTATTC GTACCGATGG
     2701 CCTTCTTTGG CGGTTCTACT GGTGCTATCT ATCGTCAGTT CTCTATTACC ATTGTTTCAG
     2761 CAATGGCGCT GTCGGTACTG GTGGCGTTGA TCCTGACTCC AGCTCTTTGT GCCACCATGC
     2821 TGAAACCGAT TGCCAAAGGC GATCACGGGG AAGGTAAAAA AGGCTTCTTC GGCTGGTTTA
     2881 ACCGCATGTT CGAGAAGAGC ACGCACCACT ACACCGACAG CGTAGGCGGT ATTCTGCGCA
     2941 GTACGGGGCG TTACCTGGTG CTGTATCTGA TCATCGTGGT CGGCATGGCC TATCTGTTCG
     3001 TGCGTCTGCC AAGCTCCTTC TTGCCAGATG AGGACCAGGG CGTGTTTATG ACCATGGTTC
     3061 AGCTGCCAGC AGGTGCAACG CAGGAACGTA CACAGAAAGT GCTCAATGAG GTAACGCATT
     3121 ACTATCTGAC CAAAGAAAAG AACAACGTTG AGTCGGTGTT CGCCGTTAAC GGCTTCGGCT
     3181 TTGCGGGACG TGGTCAGAAT ACCGGTATTG CGTTCGTTTC CTTGAAGGAC TGGGCCGATC
     3241 GTCCGGGCGA AGAAAACAAA GTTGAAGCGA TTACCATGCG TGCAACACGC GCTTTCTCGC
     3301 AAATCAAAGA TGCGATGGTT TTCGCCTTTA ACCTGCCCGC AATCGTGGAA CTGGGTACTG
     3361 CAACCGGCTT TGACTTTGAG CTGATTGACC AGGCTGGCCT TGGTCACGAA AAACTGACTC
     3421 AGGCGCGTAA CCAGTTGCTT GCAGAAGCAG CGAAGCACCC TGATATGTTG ACCAGCGTAC
     3481 GTCCAAACGG TCTGGAAGAT ACCCCGCAGT TTAAGATTGA TATCGACCAG GAAAAAGCGC
     3541 AGGCGCTGGG TGTTTCTATC AACGACATTA ACACCACTCT GGGCGCTGCA TGGGGCGGCA
     3601 GCTATGTGAA CGACTTTATC GACCGCGGTC GTGTGAAGAA AGTTTATGTC ATGTCAGAAG
     3661 CGAAATACCG TATGCTGCCG GATGATATCG GCGACTGGTA TGTTCGTGCT GCTGATGGTC
     3721 AGATGGTGCC ATTCTCGGCG TTCTCCTCTT CTCGTTGGGA GTACGGTTCG CCGCGTCTGG
     3781 AACGTTACAA CGGCCTGCCA TCCATGGAAA TCTTAGGCCA GGCGGCACCG GGTAAAAGTA
     3841 CCGGTGAAGC AATGGAGCTG ATGGAACAAC TGGCGAGCAA ACTGCCTACC GGTGTTGGCT
     3901 ATGACTGGAC GGGGATGTCC TATCAGGAAC GTCTCTCCGG CAACCAGGCA CCTTCACTGT
     3961 ACGCGATTTC GTTGATTGTC GTGTTCCTGT GTCTGGCGGC GCTGTACGAG AGCTGGTCGA
     4021 TTCCGTTCTC CGTTATGCTG GTCGTTCCGC TGGGGGTTAT CGGTGCGTTG CTGGCTGCCA
     4081 CCTTCCGTGG CCTGACCAAT GACGTTTACT TCCAGGTAGG CCTGCTCACA ACCATTGGGT
     4141 TGTCGGCGAA GAACGCGATC CTTATCGTCG AATTCGCCAA AGACTTGATG GATAAAGAAG
     4201 GTAAAGGTCT GATTGAAGCG ACGCTTGATG CGGTGCGGAT GCGTTTACGT CCGATCCTGA
     4261 TGACCTCGCT GGCGTTTATC CTCGGCGTTA TGCCGCTGGT TATCAGTACT GGTGCTGGTT
     4321 CCGGCGCGCA GAACGCAGTA GGTACCGGTG TAATGGGCGG GATGGTGACC GCAACGGTAC
     4381 TGGCAATCTT CTTCGTTCCG GTATTCTTTG TGGTGGTTCG CCGCCGCTTT AGCCGCAAGA
     4441 ATGAAGATAT CGAGCACAGC CATACTGTCG ATCATCATTT AACTTTAAAG AGGAGAAAGG
     4501 TACCGCATGC GTAAAGGCGA AGAGCTGTTC ACTGGTGTCG TCCCTATTCT GGTGGAACTG
     4561 GATGGTGATG TCAACGGTCA TAAGTTTTCC GTGCGTGGCG AGGGTGAAGG TGACGCAACT
     4621 AATGGTAAAC TGACGCTGAA GTTCATCTGT ACTACTGGTA AACTGCCGGT ACCTTGGCCG
     4681 ACTCTGGTAA CGACGCTGAC TTATGGTGTT CAGTGCTTTG CTCGTTATCC GGACCATATG
     4741 AAGCAGCATG ACTTCTTCAA GTCCGCCATG CCGGAAGGCT ATGTGCAGGA ACGCACGATT
     4801 TCCTTTAAGG ATGACGGCAC GTACAAAACG CGTGCGGAAG TGAAATTTGA AGGCGATACC
     4861 CTGGTAAACC GCATTGAGCT GAAAGGCATT GACTTTAAAG AAGACGGCAA TATCCTGGGC
     4921 CATAAGCTGG AATACAATTT TAACAGCCAC AATGTTTACA TCACCGCCGA TAAACAAAAA
     4981 AATGGCATTA AAGCGAATTT TAAAATTCGC CACAACGTGG AGGATGGCAG CGTGCAGCTG
     5041 GCTGATCACT ACCAGCAAAA CACTCCAATC GGTGATGGTC CTGTTCTGCT GCCAGACAAT
     5101 CACTATCTGA GCACGCAAAG CGTTCTGTCT AAAGATCCGA ACGAGAAACG CGATCATATG
     5161 GTTCTGCTGG AGTTCGTAAC CGCAGCGGGC ATCACGCATG GTATGGATGA ACTGTACAAA
     5221 TAATAGCATC ATCACCATCA CCACTGAGGA TCCAAACTCG AGTAAGGATC TCCAGGCATC
     5281 AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG
     5341 TGAACGCTCT CTACTAGAGT CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA
     5401 CCTAGGGATA TATTCCGCTT CCTCGCTCAC TGACTCGCTA CGCTCGGTCG TTCGACTGCG
     5461 GCGAGCGGAA ATGGCTTACG AACGGGGCGG AGATTTCCTG GAAGATGCCA GGAAGATACT
     5521 TAACAGGGAA GTGAGAGGGC CGCGGCAAAG CCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     5581 AAGCATCACG AAATCTGACG CTCAAATCAG TGGTGGCGAA ACCCGACAGG ACTATAAAGA
     5641 TACCAGGCGT TTCCCCCTGG CGGCTCCCTC GTGCGCTCTC CTGTTCCTGC CTTTCGGTTT
     5701 ACCGGTGTCA TTCCGCTGTT ATGGCCGCGT TTGTCTCATT CCACGCCTGA CACTCAGTTC
     5761 CGGGTAGGCA GTTCGCTCCA AGCTGGACTG TATGCACGAA CCCCCCGTTC AGTCCGACCG
     5821 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GAAAGACATG CAAAAGCACC
     5881 ACTGGCAGCA GCCACTGGTA ATTGATTTAG AGGAGTTAGT CTTGAAGTCA TGCGCCGGTT
     5941 AAGGCTAAAC TGAAAGGACA AGTTTTGGTG ACTGCGCTCC TCCAAGCCAG TTACCTCGGT
     6001 TCAAAGAGTT GGTAGCTCAG AGAACCTTCG AAAAACCGCC CTGCAAGGCG GTTTTTTCGT
     6061 TTTCAGAGCA AGAGATTACG CGCAGACCAA AACGATCTCA AGAAGATCAT CTTATTAATC
     6121 AGATAAAATA TTTCTAGATT TCAGTGCAAT TTATCTCTTC AAATGTAGCA CCTGAAGTCA
     6181 GCCCCATACG ATATAAGTTG TTACTAGTGC TTGGATTCTC ACCAATAAAA AACGCCCGGC
     6241 GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA GGTCATTACT GGATCTATCA
     6301 ACAGGAGTCC AAGCGAGCTC TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG
     6361 GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG
     6421 GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG
     6481 ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC
     6541 CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG
     6601 CATGCGCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC
     6661 CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG
     6721 TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC
     6781 ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC
     6841 CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC
     6901 TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CCTGCAGTTC
     6961 ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG
     7021 CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG
     7081 CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA
     7141 CGATCCTCAT CCTGTCTCTT GATCAGATCA TGATCCCCTG CGCCATCAGA TCCTTGGCGG
     7201 CAAGAAAGCC ATCCAGTTTA CTTTGCAGGG CTTCCCAACC TTACCAGAGG GCGCCCCAGC
     7261 TGGCAATTCC GACGTCGGTG CCTAATGAGT GAGCTAACTT ACATTAATTG CGTTGCGCTC
     7321 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
     7381 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA
     7441 CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA
     7501 CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC GGGATATAAC
     7561 ATGAGCTGTC TTCGGTATCG TCGTATCCCA CTACCGAGAT GTCCGCACCA ACGCGCAGCC
     7621 CGGACTCGGT AATGGCGCGC ATTGCGCCCA GCGCCATCTG ATCGTTGGCA ACCAGCATCG
     7681 CAGTGGGAAC GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG GACATGGCAC
     7741 TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA TATTTATGCC
     7801 AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG GCCCGCTAAC AGCGCGATTT
     7861 GCTGGTGACC CAATGCGACC AGATGCTCCA CGCCCAGTCG CGTACCGTCT TCATGGGAGA
     7921 AAATAATACT GTTGATGGGT GTCTGGTCAG AGACATCAAG AAATAACGCC GGAACATTAG
     7981 TGCAGGCAGC TTCCACAGCA ATGGCATCCT GGTCATCCAG CGGATAGTTA ATGATCAGCC
     8041 CACTGACGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC
     8101 GTTCTACCAT CGACACCACC ACGCTGGCAC CCAGTTGATC GGCGCGAGAT TTAATCGCCG
     8161 CGACAATTTG CGACGGCGCG TGCAGGGCCA GACTGGAGGT GGCAACGCCA ATCAGCAACG
     8221 ACTGTTTGCC CGCCAGTTGT TGTGCCACGC GGTTGGGAAT GTAATTCAGC TCCGCCATCG
     8281 CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG AAACGTGGCT GGCCTGGTTC ACCACGCGGG
     8341 AAACGGTCTG ATAAGAGACA CCGGCATACT CTGCGACATC GTATAACGTT ACTGGTTTCA
     8401 CATTCACCAC CCTGAATTGA CTCTCTTCCG GGCGCTATCA TGCCATACCG CGAAAGGTTT
     8461 TGCGCCATTC GATGGTGTCC GGGATCTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG
     8521 AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC
     8581 AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA
     8641 CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA
     8701 TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG
     8761 AGGATCGAG
//