LOCUS       Exported                5996 bp ds-DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Syn-Ace-mNeon2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Pieribone Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     609..628
                     /label=1CiVSPSynO1 seq
     primer_bind     637..670
                     /label=1AAV-Syn-Ace_BH1
     CDS             659..2095
                     /codon_start=1
                     /label=Ace-mNeon2
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSYPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVP
                     HIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGE
                     AQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTART
                     TYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYKKSRITS
                     EGEYIPLDQIDINVFCYENEV"
     primer_bind     complement(874..892)
                     /label=2Ace-79X
     primer_bind     complement(905..922)
                     /label=2_AceL89x
     primer_bind     908..945
                     /label=1-AceL89D
     primer_bind     908..945
                     /label=1-AceL89C
     primer_bind     908..945
                     /label=1-AceL89B
     primer_bind     908..945
                     /label=1-AceL89A
     primer_bind     complement(908..925)
                     /label=2_AceL90x
     primer_bind     911..948
                     /label=1_AceL90D
     primer_bind     911..948
                     /label=1_AceL90C
     primer_bind     911..948
                     /label=1_AceL90B
     primer_bind     911..948
                     /label=1_AceL90A
     primer_bind     complement(911..928)
                     /label=2_AceL91x
     primer_bind     914..953
                     /label=1_AceL91D
     primer_bind     914..953
                     /label=1_AceL91C
     primer_bind     914..953
                     /label=1_AceL91B
     primer_bind     914..953
                     /label=1_AceL91A
     primer_bind     complement(914..931)
                     /label=2_AceD92x
     primer_bind     917..955
                     /label=1_AceD92D
     primer_bind     917..955
                     /label=1_AceD92C
     primer_bind     917..955
                     /label=1_AceD92B
     primer_bind     917..955
                     /label=1_AceD92A
     primer_bind     complement(917..934)
                     /label=2_AceL93x
     primer_bind     complement(919..937)
                     /label=2_AceI94x
     primer_bind     920..957
                     /label=1_AceL93D
     primer_bind     920..957
                     /label=1_AceL93C
     primer_bind     920..957
                     /label=1_AceL93B
     primer_bind     920..957
                     /label=1_AceL93A
     primer_bind     923..959
                     /label=1_AceI94D
     primer_bind     923..959
                     /label=1_AceI94C
     primer_bind     923..959
                     /label=1_AceI94B
     primer_bind     923..959
                     /label=1_AceI94A
     primer_bind     complement(1315..1336)
                     /label=2_K227
     primer_bind     complement(1319..1339)
                     /label=2LinkerS-ins
     primer_bind     1322..1357
                     /label=1K227_D
     primer_bind     1322..1357
                     /label=1K227_C
     primer_bind     1322..1357
                     /label=1K227_B
     primer_bind     1322..1357
                     /label=1K227_A
     primer_bind     complement(1323..1345)
                     /label=2_delP230
     primer_bind     complement(1324..1345)
                     /label=2_insSYXP
     primer_bind     1325..1357
                     /label=1LinkerS-ins1
     primer_bind     complement(1325..1342)
                     /label=2LinkerG_ins
     primer_bind     complement(1327..1348)
                     /label=2_A231x
     primer_bind     1331..1374
                     /label=1_delPA
     primer_bind     1331..1369
                     /label=1P230_D
     primer_bind     1331..1369
                     /label=1P230_C
     primer_bind     1331..1369
                     /label=1P230_B
     primer_bind     1331..1369
                     /label=1P230_A
     primer_bind     1331..1369
                     /label=1_delP230
     primer_bind     1331..1365
                     /label=1_insSYXP
     primer_bind     1331..1365
                     
     primer_bind     1331..1365
                     /label=1LinkerG_ins1
     primer_bind     1334..1374
                     /label=1del_A231
     primer_bind     1334..1374
                     /label=1A231_D
     primer_bind     1334..1374
                     /label=1A231_C
     primer_bind     1334..1374
                     /label=1A231_B
     primer_bind     1334..1374
                     /label=1A231_A
     primer_bind     complement(1994..2014)
                     /label=280 - 2sfGR_AoIF2
     primer_bind     complement(1996..2014)
                     /label=2NLS-mCh
     primer_bind     complement(2077..2095)
                     /label=2AcTSER_inv
     primer_bind     complement(2080..2114)
                     /label=2AAV-Syn-TS/ER_ER1
     primer_bind     2096..2120
                     /label=1TS/ER_inv
     misc_feature    2123..2711
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2282..2307)
                     /label=WPRE-R seq
     CDS             complement(2594..2605)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2743..3219
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3197..3206)
                     /label=2_AceL93x
     repeat_region   3259..3399
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3474..3929
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4211..4315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4316..5176
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5347..5935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgccaccat
      661 ggctgacgtg gaaaccgaga ccggcatgat tgcacagtgg attgtctttg ctattatggc
      721 tgctgctgct attgcttttg gagtggctgt gcactttcgg ccttcagagc tgaagagcgc
      781 atactatatc aacattgcca tctgcactat cgccgctacc gcttactatg caatggccgt
      841 gaactaccag gacctgacaa tgaatggtga aaggcaggtg gtctacgcaa gatatatttc
      901 ctgggtgctg accacaccac tgctcctgct cgatctcatc gtcatgacca agatgggcgg
      961 agtgatgatt tcttgggtca tcggcgcaga cattttcatg atcgtgtttg gtattctggg
     1021 cgccttcgag gatgaacaca agttcaaatg ggtgtacttt atcgctggat gtgtgatgca
     1081 ggcagtcctg acatacggga tgtataacgc cacttggaaa gacgatctga agaaaagccc
     1141 cgagtaccat agctcctatg tcagtctgct cgtcttcctg tcaatcctct gggtgtttta
     1201 tcctgtcgtg tgggctttcg ggtctggtag tggcgtgctg tccgtcgaca atgaggccat
     1261 tctcatggga atcctggatg tgctcgctaa gccactgttt ggaatggggt gcctcattgc
     1321 ccatgagact atcttcaagt cctacccagc gacacatgag ttacacatct ttggctccat
     1381 caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga
     1441 ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc
     1501 tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca
     1561 ggccgccatg gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg
     1621 tgcctccctt actgttaact accgctacac ctacgaggga agccacatca aaggagaggc
     1681 ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc
     1741 tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt
     1801 taagtggagt tacaccactg gaaatggcaa gcgctacagg agcactgcgc ggaccaccta
     1861 cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg
     1921 taagacggag ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt
     1981 taccgatgtg atgggcatgg acgagctgta caagaagagc aggatcacca gcgagggcga
     2041 gtacatcccc ctggaccaga tcgacatcaa cgtgttctgc tacgagaacg aggtgtaaga
     2101 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     2161 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2221 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2281 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2341 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     2401 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     2461 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     2521 atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt
     2581 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     2641 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     2701 cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac
     2761 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     2821 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     2881 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     2941 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     3001 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     3061 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     3121 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     3181 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg
     3241 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     3301 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     3361 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     3421 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     3481 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3541 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3601 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3661 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     3721 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3781 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     3841 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3901 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     3961 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4021 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4081 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     4141 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     4201 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4261 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4321 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4381 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4441 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4501 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4561 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4621 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4681 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4741 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4801 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4861 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4921 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4981 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5041 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5101 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5161 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5221 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5281 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5341 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5401 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5461 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     5521 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5581 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5641 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5701 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5761 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5821 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5881 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5941 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
