LOCUS       Exported                6017 bp ds-DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Syn-VARNAM2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     609..628
                     /label=1CiVSPSynO1 seq
     CDS             659..2116
                     /codon_start=1
                     /label=VARNAM2
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSILIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAF
                     DILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGE
                     LVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIALKVDGGGHLHCN
                     FVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELY
                     KKSRITSEGEYIPLDQIDINVFCYENEV"
     primer_bind     complement(875..898)
                     /label=2Ace_121
     primer_bind     884..932
                     /label=1Ace_DETC
     primer_bind     884..920
                     /label=1Ace_121A
     primer_bind     complement(888..910)
                     /label=2Ace_T125
     primer_bind     901..932
                     /label=1Ace_T125C
     primer_bind     901..932
                     /label=1Ace_125A
     primer_bind     complement(1057..1078)
                     /label=2Ace_141
     primer_bind     1064..1100
                     /label=1Ace_141A
     primer_bind     complement(1265..1285)
                     /label=2Ace_A256
     primer_bind     complement(1267..1285)
                     /label=2Ace_210
     primer_bind     1271..1308
                     /label=1Ace_A256M
     primer_bind     1271..1308
                     /label=1Ace_210A
     primer_bind     complement(1317..1339)
                     /label=2Ac_lnkr
     primer_bind     1325..1381
                     /label=1AcRub_228/7
     primer_bind     1325..1379
                     /label=1AcRub_228/6
     primer_bind     1325..1376
                     /label=1AcRub_d228/5
     primer_bind     1325..1374
                     /label=1AcRub_d228/4
     primer_bind     1325..1373
                     /label=1AcRub_d228/3
     primer_bind     1325..1369
                     /label=1AcRub_d228/2
     primer_bind     1325..1365
                     /label=1AcRub_d228/1
     primer_bind     1325..1363
                     /label=1AcRub_ins228
     primer_bind     1325..1363
                     /label=1ARb_LKins2
     primer_bind     complement(1325..1342)
                     /label=2Ace-mNeon_inv
     primer_bind     1331..1366
                     /label=1Ace-mRub3
     primer_bind     complement(1741..1777)
                     /label=2mRub3_146A
     primer_bind     1763..1785
                     /label=1mRub3_146
     primer_bind     complement(1795..1831)
                     /label=2mRub3_164A
     primer_bind     1817..1835
                     /label=1mRub3_164
     primer_bind     complement(2015..2035)
                     /label=2VActm-inv
     primer_bind     complement(2017..2050)
                     /label=2Ace-mRub3
     primer_bind     complement(2017..2035)
                     /label=2mRUby2_SH1
     primer_bind     2036..2055
                     /label=1VActm-inv
     primer_bind     2036..2055
                     /label=1Ace-mNeon_inv
     primer_bind     complement(2098..2116)
                     /label=2TS/ER_inv
     primer_bind     2117..2141
                     /label=1TS/ER_inv
     misc_feature    2144..2732
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2303..2328)
                     /label=WPRE-R seq
     CDS             complement(2615..2626)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2764..3240
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3280..3420
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3495..3950
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4232..4336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4337..5197
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5368..5956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgccaccat
      661 ggctgacgtg gaaaccgaga ccggcatgat tgcacagtgg attgtctttg ctattatggc
      721 tgctgctgct attgcttttg gagtggctgt gcactttcgg ccttcagagc tgaagagcgc
      781 atactatatc aacattgcca tctgcactat cgccgctacc gcttactatg caatggccgt
      841 gaactaccag gacctgacaa tgaatggtga aaggcaggtg gtctacgcaa gatatattag
      901 ctgggtgctg accacaccac tgctcctgct cgatctcatc gtcatgacca agatgggcgg
      961 agtgatgatt tcttgggtca tcggcgcaga cattttcatg atcgtgtttg gtattctggg
     1021 cgccttcgag gatgaacaca agttcaaatg ggtgtacttt atcgctggat gtgtgatgca
     1081 ggcagtcctg acatacggga tgtataacgc cacttggaaa gacgatctga agaaaagccc
     1141 cgagtaccat agctcctatg tcagtctgct cgtcttcctg tcaatcctct gggtgtttta
     1201 tcctgtcgtg tgggctttcg ggtctggtag tggcgtgctg tccgtcgaca atgaggccat
     1261 tctcatggga atcctggatg tgctcgctaa gccactgttt ggaatggggt gcctcattgc
     1321 ccatgagact atcttcaagt ccatcctgat taaggaaaat atgcggatga aggtcgtgat
     1381 ggaagggtct gtcaatgggc accagttcaa gtgcaccgga gagggagagg gcaggccata
     1441 cgagggcgtg cagacaatga ggatcaaagt gatcgaggga ggaccactgc ctttcgcctt
     1501 tgacatcctg gccaccagct tcatgtacgg cagcaggacc ttcatcaagt atccagccga
     1561 catccccgat ttctttaagc agagcttccc cgagggcttt acctgggaga gggtgacaag
     1621 atacgaggat ggcggcgtgg tgaccgtgac acaggacacc tctctggagg atggcgagct
     1681 ggtgtataac gtgaaggtga ggggcgtgaa cttccctagc aatggcccag tgatgcagaa
     1741 gaagaccaag ggctgggagc ccaatacaga gatgatgtac cctgcagacg gaggcctgag
     1801 gggatatacc gacatcgccc tgaaggtgga tggaggagga cacctgcact gtaactttgt
     1861 gaccacatac cgctccaaga agacagtggg caatatcaag atgccaggag tgcacgccgt
     1921 ggaccacagg ctggagcgca tcgaggagtc tgataacgag acatatgtgg tgcagagaga
     1981 ggtggccgtg gccaagtact ctaatctggg cggcgggatg gacgagctgt ataagaagag
     2041 caggatcacc agcgagggcg agtacatccc cctggaccag atcgacatca acgtgttctg
     2101 ctacgagaac gaggtgtaag aattcgatat caagcttatc gataatcaac ctctggatta
     2161 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2221 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2281 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2341 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2401 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2461 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2521 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg
     2581 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2641 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     2701 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc
     2761 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     2821 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     2881 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     2941 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     3001 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     3061 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     3121 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     3181 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     3241 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     3301 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     3361 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     3421 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     3481 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3541 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3601 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3661 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     3721 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3781 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     3841 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3901 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     3961 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     4021 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4081 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     4141 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4201 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4261 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4321 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4381 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4441 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4501 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4561 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4621 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4681 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4741 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4801 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4861 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4921 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4981 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5041 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5101 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5161 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5221 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5281 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5341 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5401 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5461 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5521 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5581 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5641 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5701 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5761 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5821 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5881 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5941 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6001 gccttttgct cacatgt
//
