LOCUS       Exported                6194 bp ds-DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Syn-pAce-Kv2.1PR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Pieribone Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     609..628
                     /label=1CiVSPSynO1 seq
     primer_bind     632..671
                     /label=1Syn-PACE_BH1
     primer_bind     637..670
                     /label=1AAV-Syn-Ace_BH1
     CDS             659..2290
                     /codon_start=1
                     /label=pAce-Kv2.1PR
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYAKYIDWVLTTPLLLLNLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILFVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSYPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVP
                     HIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGE
                     AQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTART
                     TYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYKKSRITS
                     EGEYIPLDQIDINVFCYENEVQSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTE
                     GVIDMRSMSSIDSFISCATDFPEATRF"
     primer_bind     complement(874..890)
                     /label=2Ace-79X
     primer_bind     complement(905..922)
                     /label=2_AceL89x
     primer_bind     908..945
                     /label=1-AceL89D
     primer_bind     908..945
                     /label=1-AceL89C
     primer_bind     908..945
                     /label=1-AceL89B
     primer_bind     908..945
                     /label=1-AceL89A
     primer_bind     complement(908..925)
                     /label=2_AceL90x
     primer_bind     911..948
                     /label=1_AceL90D
     primer_bind     911..948
                     /label=1_AceL90C
     primer_bind     911..948
                     /label=1_AceL90B
     primer_bind     911..948
                     /label=1_AceL90A
     primer_bind     complement(911..928)
                     /label=2_AceL91x
     primer_bind     914..953
                     /label=1_AceL91D
     primer_bind     914..953
                     /label=1_AceL91C
     primer_bind     914..953
                     /label=1_AceL91B
     primer_bind     914..953
                     /label=1_AceL91A
     primer_bind     complement(914..931)
                     /label=2_AceD92x
     primer_bind     917..955
                     /label=1_AceD92D
     primer_bind     917..955
                     /label=1_AceD92C
     primer_bind     917..955
                     /label=1_AceD92B
     primer_bind     917..955
                     /label=1_AceD92A
     primer_bind     complement(917..931)
                     /label=2_AceL93x
     primer_bind     complement(919..931)
                     /label=2_AceI94x
     primer_bind     920..957
                     /label=1_AceL93D
     primer_bind     920..957
                     /label=1_AceL93C
     primer_bind     920..957
                     /label=1_AceL93B
     primer_bind     920..957
                     /label=1_AceL93A
     primer_bind     923..959
                     /label=1_AceI94D
     primer_bind     923..959
                     /label=1_AceI94C
     primer_bind     923..959
                     /label=1_AceI94B
     primer_bind     923..959
                     /label=1_AceI94A
     primer_bind     complement(1315..1336)
                     /label=2_K227
     primer_bind     complement(1319..1339)
                     /label=2LinkerS-ins
     primer_bind     1322..1357
                     /label=1K227_D
     primer_bind     1322..1357
                     /label=1K227_C
     primer_bind     1322..1357
                     /label=1K227_B
     primer_bind     1322..1357
                     /label=1K227_A
     primer_bind     complement(1323..1345)
                     /label=2_delP230
     primer_bind     complement(1324..1345)
                     /label=2_insSYXP
     primer_bind     1325..1357
                     /label=1LinkerS-ins1
     primer_bind     complement(1325..1342)
                     /label=2LinkerG_ins
     primer_bind     complement(1327..1348)
                     /label=2_A231x
     primer_bind     1331..1374
                     /label=1_delPA
     primer_bind     1331..1369
                     /label=1P230_D
     primer_bind     1331..1369
                     /label=1P230_C
     primer_bind     1331..1369
                     /label=1P230_B
     primer_bind     1331..1369
                     /label=1P230_A
     primer_bind     1331..1369
                     /label=1_delP230
     primer_bind     1331..1365
                     /label=1_insSYXP
     primer_bind     1331..1365
                     
     primer_bind     1331..1365
                     /label=1LinkerG_ins1
     primer_bind     1334..1374
                     /label=1del_A231
     primer_bind     1334..1374
                     /label=1A231_D
     primer_bind     1334..1374
                     /label=1A231_C
     primer_bind     1334..1374
                     /label=1A231_B
     primer_bind     1334..1374
                     /label=1A231_A
     primer_bind     complement(1994..2024)
                     /label=2PACE
     primer_bind     complement(1994..2014)
                     /label=280 - 2sfGR_AoIF2
     primer_bind     complement(1996..2014)
                     /label=2NLS-mCh
     primer_bind     2005..2035
                     /label=1PACE-Kv
     primer_bind     complement(2077..2095)
                     /label=2AcTSER_inv
     primer_bind     complement(2080..2095)
                     /label=2AAV-Syn-TS/ER_ER1
     primer_bind     2081..2119
                     /label=1Kv2.1px
     primer_bind     complement(2271..2308)
                     /label=2Kv2.1px
     primer_bind     complement(2272..2317)
                     /label=2PACE-Kv_ER1
     misc_feature    2321..2909
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2480..2505)
                     /label=WPRE-R seq
     CDS             complement(2792..2803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2941..3417
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3395..3404)
                     /label=2_AceL93x
     repeat_region   3457..3597
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3672..4127
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4409..4513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4514..5374
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5545..6133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgccaccat
      661 ggctgacgtg gaaaccgaga ccggcatgat tgcacagtgg attgtctttg ctattatggc
      721 tgctgctgct attgcttttg gagtggctgt gcactttcgg ccttcagagc tgaagagcgc
      781 atactatatc aacattgcca tctgcactat cgccgctacc gcttactatg caatggccgt
      841 gaactaccag gacctgacaa tgaatggtga aaggcaggtg gtctacgcaa agtatattga
      901 ctgggtgctg accacaccac tgctcctgct caacctcatc gtcatgacca agatgggcgg
      961 agtgatgatt tcttgggtca tcggcgcaga cattttcatg atcgtgtttg gtattctggg
     1021 cgccttcgag gatgaacaca agttcaaatg ggtgtacttt atcgctggat gtgtgatgca
     1081 ggcagtcctg acatacggga tgtataacgc cacttggaaa gacgatctga agaaaagccc
     1141 cgagtaccat agctcctatg tcagtctgct cgtcttcctg tcaatcctct tcgtgtttta
     1201 tcctgtcgtg tgggctttcg ggtctggtag tggcgtgctg tccgtcgaca atgaggccat
     1261 tctcatggga atcctggatg tgctcgctaa gccactgttt ggaatggggt gcctcattgc
     1321 ccatgagact atcttcaagt cctacccagc gacacatgag ttacacatct ttggctccat
     1381 caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga
     1441 ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc
     1501 tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca
     1561 ggccgccatg gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg
     1621 tgcctccctt actgttaact accgctacac ctacgaggga agccacatca aaggagaggc
     1681 ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc
     1741 tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt
     1801 taagtggagt tacaccactg gaaatggcaa gcgctacagg agcactgcgc ggaccaccta
     1861 cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg
     1921 taagacggag ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt
     1981 taccgatgtg atgggcatgg acgagctgta caagaagagc aggatcacca gcgagggcga
     2041 gtacatcccc ctggaccaga tcgacatcaa cgtgttctgc tacgagaacg aggtgcaaag
     2101 tcagcctatc ctgaacacaa aggaaatggc tccacagtct aagcctcccg aagagcttga
     2161 gatgtccagt atgccaagtc ccgtggctcc cctccctgcc aggactgaag gagtgattga
     2221 catgaggagt atgtcatcta ttgatagctt catctcttgc gcaacagatt tccccgaggc
     2281 tactcgattc taataagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     2341 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2401 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2461 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2521 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2581 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2641 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2701 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat
     2761 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2821 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2881 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     2941 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     3001 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     3061 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     3121 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     3181 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3241 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3301 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3361 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     3421 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3481 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3541 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3601 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     3661 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3721 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3781 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3841 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     3901 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3961 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     4021 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4081 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4141 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4201 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4261 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4321 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4381 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4501 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4561 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4621 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4681 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4741 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4801 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4861 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4921 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4981 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5041 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5101 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5161 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5221 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5281 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5341 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5401 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5461 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6061 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6121 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6181 ttttgctcac atgt
//
