LOCUS       Exported                6215 bp ds-DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Syn-VARNAM2-Kv2.1PR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     609..628
                     /label=1CiVSPSynO1 seq
     CDS             659..2311
                     /codon_start=1
                     /label=VARNAM2-Kv2.1PR
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSILIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAF
                     DILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGE
                     LVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIALKVDGGGHLHCN
                     FVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELY
                     KKSRITSEGEYIPLDQIDINVFCYENEVQSQPILNTKEMAPQSKPPEELEMSSMPSPVA
                     PLPARTEGVIDMRSMSSIDSFISCATDFPEATRF"
     primer_bind     complement(875..898)
                     /label=2Ace_121
     primer_bind     884..932
                     /label=1Ace_DETC
     primer_bind     884..920
                     /label=1Ace_121A
     primer_bind     complement(888..910)
                     /label=2Ace_T125
     primer_bind     901..932
                     /label=1Ace_T125C
     primer_bind     901..932
                     /label=1Ace_125A
     primer_bind     complement(1057..1078)
                     /label=2Ace_141
     primer_bind     1064..1100
                     /label=1Ace_141A
     primer_bind     complement(1265..1285)
                     /label=2Ace_A256
     primer_bind     complement(1267..1285)
                     /label=2Ace_210
     primer_bind     1271..1308
                     /label=1Ace_A256M
     primer_bind     1271..1308
                     /label=1Ace_210A
     primer_bind     complement(1317..1339)
                     /label=2Ac_lnkr
     primer_bind     1325..1381
                     /label=1AcRub_228/7
     primer_bind     1325..1379
                     /label=1AcRub_228/6
     primer_bind     1325..1376
                     /label=1AcRub_d228/5
     primer_bind     1325..1374
                     /label=1AcRub_d228/4
     primer_bind     1325..1373
                     /label=1AcRub_d228/3
     primer_bind     1325..1369
                     /label=1AcRub_d228/2
     primer_bind     1325..1365
                     /label=1AcRub_d228/1
     primer_bind     1325..1363
                     /label=1AcRub_ins228
     primer_bind     1325..1363
                     /label=1ARb_LKins2
     primer_bind     complement(1325..1342)
                     /label=2Ace-mNeon_inv
     primer_bind     1331..1366
                     /label=1Ace-mRub3
     primer_bind     complement(1741..1777)
                     /label=2mRub3_146A
     primer_bind     1763..1785
                     /label=1mRub3_146
     primer_bind     complement(1795..1831)
                     /label=2mRub3_164A
     primer_bind     1817..1835
                     /label=1mRub3_164
     primer_bind     complement(2015..2035)
                     /label=2VActm-inv
     primer_bind     complement(2017..2050)
                     /label=2Ace-mRub3
     primer_bind     complement(2017..2035)
                     /label=2mRUby2_SH1
     primer_bind     2036..2055
                     /label=1VActm-inv
     primer_bind     2036..2055
                     /label=1Ace-mNeon_inv
     primer_bind     complement(2098..2116)
                     /label=2TS/ER_inv
     primer_bind     2102..2140
                     
     primer_bind     2102..2140
                     /label=1Kv2.1px
     primer_bind     complement(2292..2329)
                     
     primer_bind     complement(2292..2329)
                     /label=2Kv2.1px
     primer_bind     complement(2293..2314)
                     /label=1DIO-ANv3-Kv2.1
     primer_bind     2315..2339
                     /label=1TS/ER_inv
     misc_feature    2342..2930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2501..2526)
                     /label=WPRE-R seq
     CDS             complement(2813..2824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2962..3438
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3478..3618
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3693..4148
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4430..4534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4535..5395
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5566..6154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ccgccaccat
      661 ggctgacgtg gaaaccgaga ccggcatgat tgcacagtgg attgtctttg ctattatggc
      721 tgctgctgct attgcttttg gagtggctgt gcactttcgg ccttcagagc tgaagagcgc
      781 atactatatc aacattgcca tctgcactat cgccgctacc gcttactatg caatggccgt
      841 gaactaccag gacctgacaa tgaatggtga aaggcaggtg gtctacgcaa gatatattag
      901 ctgggtgctg accacaccac tgctcctgct cgatctcatc gtcatgacca agatgggcgg
      961 agtgatgatt tcttgggtca tcggcgcaga cattttcatg atcgtgtttg gtattctggg
     1021 cgccttcgag gatgaacaca agttcaaatg ggtgtacttt atcgctggat gtgtgatgca
     1081 ggcagtcctg acatacggga tgtataacgc cacttggaaa gacgatctga agaaaagccc
     1141 cgagtaccat agctcctatg tcagtctgct cgtcttcctg tcaatcctct gggtgtttta
     1201 tcctgtcgtg tgggctttcg ggtctggtag tggcgtgctg tccgtcgaca atgaggccat
     1261 tctcatggga atcctggatg tgctcgctaa gccactgttt ggaatggggt gcctcattgc
     1321 ccatgagact atcttcaagt ccatcctgat taaggaaaat atgcggatga aggtcgtgat
     1381 ggaagggtct gtcaatgggc accagttcaa gtgcaccgga gagggagagg gcaggccata
     1441 cgagggcgtg cagacaatga ggatcaaagt gatcgaggga ggaccactgc ctttcgcctt
     1501 tgacatcctg gccaccagct tcatgtacgg cagcaggacc ttcatcaagt atccagccga
     1561 catccccgat ttctttaagc agagcttccc cgagggcttt acctgggaga gggtgacaag
     1621 atacgaggat ggcggcgtgg tgaccgtgac acaggacacc tctctggagg atggcgagct
     1681 ggtgtataac gtgaaggtga ggggcgtgaa cttccctagc aatggcccag tgatgcagaa
     1741 gaagaccaag ggctgggagc ccaatacaga gatgatgtac cctgcagacg gaggcctgag
     1801 gggatatacc gacatcgccc tgaaggtgga tggaggagga cacctgcact gtaactttgt
     1861 gaccacatac cgctccaaga agacagtggg caatatcaag atgccaggag tgcacgccgt
     1921 ggaccacagg ctggagcgca tcgaggagtc tgataacgag acatatgtgg tgcagagaga
     1981 ggtggccgtg gccaagtact ctaatctggg cggcgggatg gacgagctgt ataagaagag
     2041 caggatcacc agcgagggcg agtacatccc cctggaccag atcgacatca acgtgttctg
     2101 ctacgagaac gaggtgcaaa gtcagcctat cctgaacaca aaggaaatgg ctccacagtc
     2161 taagcctccc gaagagcttg agatgtccag tatgccaagt cccgtggctc ccctccctgc
     2221 caggactgaa ggagtgattg acatgaggag tatgtcatct attgatagct tcatctcttg
     2281 cgcaacagat ttccccgagg ctactcgatt ctaataagaa ttcgatatca agcttatcga
     2341 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2401 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2461 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2521 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2581 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2641 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2701 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2761 cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2821 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2881 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     2941 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     3001 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     3061 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3121 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3181 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3241 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     3301 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     3361 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     3421 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     3481 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3541 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3601 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3661 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3781 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3841 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3901 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3961 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4021 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4081 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4141 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4201 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4261 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4321 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4381 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4441 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4501 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4561 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4621 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4681 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4741 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4801 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4861 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4921 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4981 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5041 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5101 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5161 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5221 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5281 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5341 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5401 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5461 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5521 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5701 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5941 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6181 gttcctggcc ttttgctggc cttttgctca catgt
//
