LOCUS       Exported                6794 bp ds-DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CaMKII-Ace-mNeon2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Pieribone Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     1443..1480
                     /label=1CaM-Ac-KPN
     primer_bind     1456..1480
                     /label=1AAV-Syn-Ace_BH1
     CDS             1469..2905
                     /codon_start=1
                     /label=Ace-mNeon2
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSYPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVP
                     HIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGE
                     AQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTART
                     TYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYKKSRITS
                     EGEYIPLDQIDINVFCYENEV"
     primer_bind     complement(1684..1702)
                     /label=2Ace-79X
     primer_bind     complement(1715..1732)
                     /label=2_AceL89x
     primer_bind     1718..1755
                     /label=1-AceL89D
     primer_bind     1718..1755
                     /label=1-AceL89C
     primer_bind     1718..1755
                     /label=1-AceL89B
     primer_bind     1718..1755
                     /label=1-AceL89A
     primer_bind     complement(1718..1735)
                     /label=2_AceL90x
     primer_bind     1721..1758
                     /label=1_AceL90D
     primer_bind     1721..1758
                     /label=1_AceL90C
     primer_bind     1721..1758
                     /label=1_AceL90B
     primer_bind     1721..1758
                     /label=1_AceL90A
     primer_bind     complement(1721..1738)
                     /label=2_AceL91x
     primer_bind     1724..1763
                     /label=1_AceL91D
     primer_bind     1724..1763
                     /label=1_AceL91C
     primer_bind     1724..1763
                     /label=1_AceL91B
     primer_bind     1724..1763
                     /label=1_AceL91A
     primer_bind     complement(1724..1741)
                     /label=2_AceD92x
     primer_bind     1727..1765
                     /label=1_AceD92D
     primer_bind     1727..1765
                     /label=1_AceD92C
     primer_bind     1727..1765
                     /label=1_AceD92B
     primer_bind     1727..1765
                     /label=1_AceD92A
     primer_bind     complement(1727..1744)
                     /label=2_AceL93x
     primer_bind     complement(1729..1747)
                     /label=2_AceI94x
     primer_bind     1730..1767
                     /label=1_AceL93D
     primer_bind     1730..1767
                     /label=1_AceL93C
     primer_bind     1730..1767
                     /label=1_AceL93B
     primer_bind     1730..1767
                     /label=1_AceL93A
     primer_bind     1733..1769
                     /label=1_AceI94D
     primer_bind     1733..1769
                     /label=1_AceI94C
     primer_bind     1733..1769
                     /label=1_AceI94B
     primer_bind     1733..1769
                     /label=1_AceI94A
     primer_bind     complement(2125..2146)
                     /label=2_K227
     primer_bind     complement(2129..2149)
                     /label=2LinkerS-ins
     primer_bind     2132..2167
                     /label=1K227_D
     primer_bind     2132..2167
                     /label=1K227_C
     primer_bind     2132..2167
                     /label=1K227_B
     primer_bind     2132..2167
                     /label=1K227_A
     primer_bind     complement(2133..2155)
                     /label=2_delP230
     primer_bind     complement(2134..2155)
                     /label=2_insSYXP
     primer_bind     2135..2167
                     /label=1LinkerS-ins1
     primer_bind     complement(2135..2152)
                     /label=2LinkerG_ins
     primer_bind     complement(2137..2158)
                     /label=2_A231x
     primer_bind     2141..2184
                     /label=1_delPA
     primer_bind     2141..2179
                     /label=1P230_D
     primer_bind     2141..2179
                     /label=1P230_C
     primer_bind     2141..2179
                     /label=1P230_B
     primer_bind     2141..2179
                     /label=1P230_A
     primer_bind     2141..2179
                     /label=1_delP230
     primer_bind     2141..2175
                     /label=1_insSYXP
     primer_bind     2141..2175
                     
     primer_bind     2141..2175
                     /label=1LinkerG_ins1
     primer_bind     2144..2184
                     /label=1del_A231
     primer_bind     2144..2184
                     /label=1A231_D
     primer_bind     2144..2184
                     /label=1A231_C
     primer_bind     2144..2184
                     /label=1A231_B
     primer_bind     2144..2184
                     /label=1A231_A
     primer_bind     complement(2804..2824)
                     /label=280 - 2sfGR_AoIF2
     primer_bind     complement(2806..2824)
                     /label=2NLS-mCh
     primer_bind     complement(2886..2905)
                     /label=2CaMAce-noKv
     primer_bind     complement(2887..2905)
                     /label=2AcTSER_inv
     primer_bind     complement(2890..2908)
                     /label=2AAV-Syn-TS/ER_ER1
     primer_bind     2891..2930
                     /label=1CaMAce-noKv
     misc_feature    2921..3509
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3392..3403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3541..4017
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3995..4004)
                     /label=2_AceL93x
     repeat_region   4057..4197
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4272..4727
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5009..5113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5114..5974
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6145..6733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccgccaccat ggctgacgtg gaaaccgaga ccggcatgat
     1501 tgcacagtgg attgtctttg ctattatggc tgctgctgct attgcttttg gagtggctgt
     1561 gcactttcgg ccttcagagc tgaagagcgc atactatatc aacattgcca tctgcactat
     1621 cgccgctacc gcttactatg caatggccgt gaactaccag gacctgacaa tgaatggtga
     1681 aaggcaggtg gtctacgcaa gatatatttc ctgggtgctg accacaccac tgctcctgct
     1741 cgatctcatc gtcatgacca agatgggcgg agtgatgatt tcttgggtca tcggcgcaga
     1801 cattttcatg atcgtgtttg gtattctggg cgccttcgag gatgaacaca agttcaaatg
     1861 ggtgtacttt atcgctggat gtgtgatgca ggcagtcctg acatacggga tgtataacgc
     1921 cacttggaaa gacgatctga agaaaagccc cgagtaccat agctcctatg tcagtctgct
     1981 cgtcttcctg tcaatcctct gggtgtttta tcctgtcgtg tgggctttcg ggtctggtag
     2041 tggcgtgctg tccgtcgaca atgaggccat tctcatggga atcctggatg tgctcgctaa
     2101 gccactgttt ggaatggggt gcctcattgc ccatgagact atcttcaagt cctacccagc
     2161 gacacatgag ttacacatct ttggctccat caacggtgtg gactttgaca tggtgggtca
     2221 gggcaccggc aatccaaatg atggttatga ggagttaaac ctgaagtcca ccaagggtga
     2281 cctccagttc tccccctgga ttctggtccc tcatatcggg tatggcttcc atcagtacct
     2341 gccctaccct gacgggatgt cgcctttcca ggccgccatg gtagatggct ccggatacca
     2401 agtccatcgc acaatgcagt ttgaagatgg tgcctccctt actgttaact accgctacac
     2461 ctacgaggga agccacatca aaggagaggc ccaggtgaag gggactggtt tccctgctga
     2521 cggtcctgtg atgaccaact cgctgaccgc tgcggactgg tgcaggtcga agaagactta
     2581 ccccaacgac aaaaccatca tcagtacctt taagtggagt tacaccactg gaaatggcaa
     2641 gcgctacagg agcactgcgc ggaccaccta cacctttgcc aagccaatgg cggctaacta
     2701 tctgaagaac cagccgatgt acgtgttccg taagacggag ctcaagcact ccaagaccga
     2761 gctcaacttc aaggagtggc aaaaggcctt taccgatgtg atgggcatgg acgagctgta
     2821 caagaagagc aggatcacca gcgagggcga gtacatcccc ctggaccaga tcgacatcaa
     2881 cgtgttctgc tacgagaacg aggtgtaaaa gcttatcgat aatcaacctc tggattacaa
     2941 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3001 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3061 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3121 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3181 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3241 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3301 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3361 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3421 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3481 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     3541 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     3601 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     3661 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     3721 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     3781 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3841 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3901 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3961 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     4021 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     4081 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     4141 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     4201 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4261 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4321 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4381 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4441 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     4501 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4561 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     4621 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4681 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4741 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4801 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4861 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4921 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4981 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5041 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5101 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5161 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5221 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5281 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5341 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5401 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5461 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5521 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5581 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5641 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5701 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5761 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5821 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5881 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5941 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6001 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6061 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     6301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6781 ttttgctcac atgt
//
