LOCUS       Exported                6815 bp ds-DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CaMKII-VARNAM2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Pieribone Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(405..416)
                     /label=1mRub3_146
     primer_bind     1443..1480
                     /label=1CaM-Ac-KPN
     primer_bind     1456..1480
                     /label=1VA_NLS
     CDS             1469..2926
                     /codon_start=1
                     /label=VARNAM2
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSILIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAF
                     DILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGE
                     LVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIALKVDGGGHLHCN
                     FVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELY
                     KKSRITSEGEYIPLDQIDINVFCYENEV"
     primer_bind     complement(1685..1708)
                     /label=2Ace_121
     primer_bind     1694..1742
                     /label=1Ace_DETC
     primer_bind     1694..1730
                     /label=1Ace_121A
     primer_bind     complement(1698..1720)
                     /label=2Ace_T125
     primer_bind     1711..1742
                     /label=1Ace_T125C
     primer_bind     1711..1742
                     /label=1Ace_125A
     primer_bind     complement(1727..1744)
                     /label=2_AceL93x
     primer_bind     complement(2075..2095)
                     /label=2Ace_A256
     primer_bind     2081..2118
                     /label=1Ace_A256M
     primer_bind     complement(2127..2149)
                     /label=2Ac_lnkr
     primer_bind     complement(2131..2152)
                     /label=2GLinker
     primer_bind     2135..2191
                     /label=1AcRub_228/7
     primer_bind     2135..2189
                     /label=1AcRub_228/6
     primer_bind     2135..2186
                     /label=1AcRub_d228/5
     primer_bind     2135..2184
                     /label=1AcRub_d228/4
     primer_bind     2135..2183
                     /label=1AcRub_d228/3
     primer_bind     2135..2179
                     /label=1AcRub_d228/2
     primer_bind     2135..2175
                     /label=1AcRub_d228/1
     primer_bind     2135..2173
                     /label=1AcRub_ins228
     primer_bind     2135..2173
                     /label=1ARb_LKins2
     primer_bind     complement(2135..2152)
                     /label=2Ace-mNeon_inv
     primer_bind     2138..2179
                     /label=1LinkerG_A
     primer_bind     2138..2179
                     /label=1LinkerG_B
     primer_bind     2138..2179
                     /label=1LinkerG_C
     primer_bind     2138..2179
                     /label=1LinkerG_D
     primer_bind     2138..2179
                     /label=1GLinkerY
     primer_bind     2138..2179
                     /label=1GLinkerH
     primer_bind     2138..2179
                     /label=1GLinkerV
     primer_bind     2141..2176
                     /label=1Ace-mRub3
     primer_bind     complement(2551..2587)
                     /label=2mRub3_146A
     primer_bind     complement(2557..2572)
                     /label=2Ruby-N147
     primer_bind     2558..2596
                     /label=1RubyN147_A
     primer_bind     2558..2596
                     /label=1RubyN147_B
     primer_bind     2558..2596
                     /label=1RubyN147_C
     primer_bind     2558..2596
                     /label=1RubyN147_D
     primer_bind     2573..2595
                     /label=1mRub3_146
     primer_bind     complement(2605..2641)
                     /label=2mRub3_164A
     primer_bind     2627..2645
                     /label=1mRub3_164
     primer_bind     complement(2827..2860)
                     /label=2Ace-mRub3
     primer_bind     complement(2827..2845)
                     /label=2mRUby2_SH1
     primer_bind     2846..2865
                     /label=1Ace-mNeon_inv
     primer_bind     complement(2908..2926)
                     /label=2TS/ER_inv
     primer_bind     complement(2909..2926)
                     /label=2VA_NLS
     misc_feature    2942..3530
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3413..3424)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3562..4038
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4016..4025)
                     /label=2_AceL93x
     repeat_region   4078..4218
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4293..4748
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5030..5134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5135..5995
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6166..6754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccgccaccat ggctgacgtg gaaaccgaga ccggcatgat
     1501 tgcacagtgg attgtctttg ctattatggc tgctgctgct attgcttttg gagtggctgt
     1561 gcactttcgg ccttcagagc tgaagagcgc atactatatc aacattgcca tctgcactat
     1621 cgccgctacc gcttactatg caatggccgt gaactaccag gacctgacaa tgaatggtga
     1681 aaggcaggtg gtctacgcaa gatatattag ctgggtgctg accacaccac tgctcctgct
     1741 cgatctcatc gtcatgacca agatgggcgg agtgatgatt tcttgggtca tcggcgcaga
     1801 cattttcatg atcgtgtttg gtattctggg cgccttcgag gatgaacaca agttcaaatg
     1861 ggtgtacttt atcgctggat gtgtgatgca ggcagtcctg acatacggga tgtataacgc
     1921 cacttggaaa gacgatctga agaaaagccc cgagtaccat agctcctatg tcagtctgct
     1981 cgtcttcctg tcaatcctct gggtgtttta tcctgtcgtg tgggctttcg ggtctggtag
     2041 tggcgtgctg tccgtcgaca atgaggccat tctcatggga atcctggatg tgctcgctaa
     2101 gccactgttt ggaatggggt gcctcattgc ccatgagact atcttcaagt ccatcctgat
     2161 taaggaaaat atgcggatga aggtcgtgat ggaagggtct gtcaatgggc accagttcaa
     2221 gtgcaccgga gagggagagg gcaggccata cgagggcgtg cagacaatga ggatcaaagt
     2281 gatcgaggga ggaccactgc ctttcgcctt tgacatcctg gccaccagct tcatgtacgg
     2341 cagcaggacc ttcatcaagt atccagccga catccccgat ttctttaagc agagcttccc
     2401 cgagggcttt acctgggaga gggtgacaag atacgaggat ggcggcgtgg tgaccgtgac
     2461 acaggacacc tctctggagg atggcgagct ggtgtataac gtgaaggtga ggggcgtgaa
     2521 cttccctagc aatggcccag tgatgcagaa gaagaccaag ggctgggagc ccaatacaga
     2581 gatgatgtac cctgcagacg gaggcctgag gggatatacc gacatcgccc tgaaggtgga
     2641 tggaggagga cacctgcact gtaactttgt gaccacatac cgctccaaga agacagtggg
     2701 caatatcaag atgccaggag tgcacgccgt ggaccacagg ctggagcgca tcgaggagtc
     2761 tgataacgag acatatgtgg tgcagagaga ggtggccgtg gccaagtact ctaatctggg
     2821 cggcgggatg gacgagctgt ataagaagag caggatcacc agcgagggcg agtacatccc
     2881 cctggaccag atcgacatca acgtgttctg ctacgagaac gaggtgtaaa agcttatcga
     2941 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3001 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3061 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3121 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3181 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3241 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3301 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3361 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3421 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3481 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3541 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     3601 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     3661 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3721 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3781 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3841 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     3901 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     3961 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     4021 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     4081 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4141 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4201 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4261 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4321 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4381 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4441 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4501 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4561 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4621 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4681 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4741 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4801 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4861 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4921 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4981 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5041 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5101 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5161 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5221 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5281 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5341 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5401 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5461 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5521 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5581 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5641 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5701 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5761 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5821 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5881 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5941 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6001 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6301 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6781 gttcctggcc ttttgctggc cttttgctca catgt
//
