LOCUS       Exported                6989 bp ds-DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CaMKII-Ace-mNeon2-Kv2.1PR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Pieribone Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     1443..1480
                     /label=1CaM-Ac-KPN
     primer_bind     1456..1480
                     /label=1AAV-Syn-Ace_BH1
     CDS             1469..3103
                     /codon_start=1
                     /label=Ace-mNeon2-Kv2.1PR
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSYPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVP
                     HIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGE
                     AQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTART
                     TYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYKKSRITS
                     EGEYIPLDQIDINVFCYENEVQSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTE
                     GVIDMRSMSSIDSFISCATDFPEATRF"
     primer_bind     complement(1684..1702)
                     /label=2Ace-79X
     primer_bind     complement(1715..1732)
                     /label=2_AceL89x
     primer_bind     1718..1755
                     /label=1-AceL89D
     primer_bind     1718..1755
                     /label=1-AceL89C
     primer_bind     1718..1755
                     /label=1-AceL89B
     primer_bind     1718..1755
                     /label=1-AceL89A
     primer_bind     complement(1718..1735)
                     /label=2_AceL90x
     primer_bind     1721..1758
                     /label=1_AceL90D
     primer_bind     1721..1758
                     /label=1_AceL90C
     primer_bind     1721..1758
                     /label=1_AceL90B
     primer_bind     1721..1758
                     /label=1_AceL90A
     primer_bind     complement(1721..1738)
                     /label=2_AceL91x
     primer_bind     1724..1763
                     /label=1_AceL91D
     primer_bind     1724..1763
                     /label=1_AceL91C
     primer_bind     1724..1763
                     /label=1_AceL91B
     primer_bind     1724..1763
                     /label=1_AceL91A
     primer_bind     complement(1724..1741)
                     /label=2_AceD92x
     primer_bind     1727..1765
                     /label=1_AceD92D
     primer_bind     1727..1765
                     /label=1_AceD92C
     primer_bind     1727..1765
                     /label=1_AceD92B
     primer_bind     1727..1765
                     /label=1_AceD92A
     primer_bind     complement(1727..1744)
                     /label=2_AceL93x
     primer_bind     complement(1729..1747)
                     /label=2_AceI94x
     primer_bind     1730..1767
                     /label=1_AceL93D
     primer_bind     1730..1767
                     /label=1_AceL93C
     primer_bind     1730..1767
                     /label=1_AceL93B
     primer_bind     1730..1767
                     /label=1_AceL93A
     primer_bind     1733..1769
                     /label=1_AceI94D
     primer_bind     1733..1769
                     /label=1_AceI94C
     primer_bind     1733..1769
                     /label=1_AceI94B
     primer_bind     1733..1769
                     /label=1_AceI94A
     primer_bind     complement(2125..2146)
                     /label=2_K227
     primer_bind     complement(2129..2149)
                     /label=2LinkerS-ins
     primer_bind     2132..2167
                     /label=1K227_D
     primer_bind     2132..2167
                     /label=1K227_C
     primer_bind     2132..2167
                     /label=1K227_B
     primer_bind     2132..2167
                     /label=1K227_A
     primer_bind     complement(2133..2155)
                     /label=2_delP230
     primer_bind     complement(2134..2155)
                     /label=2_insSYXP
     primer_bind     2135..2167
                     /label=1LinkerS-ins1
     primer_bind     complement(2135..2152)
                     /label=2LinkerG_ins
     primer_bind     complement(2137..2158)
                     /label=2_A231x
     primer_bind     2141..2184
                     /label=1_delPA
     primer_bind     2141..2179
                     /label=1P230_D
     primer_bind     2141..2179
                     /label=1P230_C
     primer_bind     2141..2179
                     /label=1P230_B
     primer_bind     2141..2179
                     /label=1P230_A
     primer_bind     2141..2179
                     /label=1_delP230
     primer_bind     2141..2175
                     /label=1_insSYXP
     primer_bind     2141..2175
                     
     primer_bind     2141..2175
                     /label=1LinkerG_ins1
     primer_bind     2144..2184
                     /label=1del_A231
     primer_bind     2144..2184
                     /label=1A231_D
     primer_bind     2144..2184
                     /label=1A231_C
     primer_bind     2144..2184
                     /label=1A231_B
     primer_bind     2144..2184
                     /label=1A231_A
     primer_bind     complement(2804..2824)
                     /label=280 - 2sfGR_AoIF2
     primer_bind     complement(2806..2824)
                     /label=2NLS-mCh
     primer_bind     complement(2886..2905)
                     /label=2CaMAce-noKv
     primer_bind     complement(2887..2905)
                     /label=2AcTSER_inv
     primer_bind     complement(2890..2905)
                     /label=2AAV-Syn-TS/ER_ER1
     primer_bind     2891..2929
                     /label=1Kv2.1px
     primer_bind     complement(3081..3103)
                     /label=2Kv2.1px
     primer_bind     3101..3125
                     /label=1CaMAce-noKv
     misc_feature    3116..3704
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3587..3598)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3736..4212
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4190..4199)
                     /label=2_AceL93x
     repeat_region   4252..4392
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4467..4922
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5204..5308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5309..6169
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6340..6928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccgccaccat ggctgacgtg gaaaccgaga ccggcatgat
     1501 tgcacagtgg attgtctttg ctattatggc tgctgctgct attgcttttg gagtggctgt
     1561 gcactttcgg ccttcagagc tgaagagcgc atactatatc aacattgcca tctgcactat
     1621 cgccgctacc gcttactatg caatggccgt gaactaccag gacctgacaa tgaatggtga
     1681 aaggcaggtg gtctacgcaa gatatatttc ctgggtgctg accacaccac tgctcctgct
     1741 cgatctcatc gtcatgacca agatgggcgg agtgatgatt tcttgggtca tcggcgcaga
     1801 cattttcatg atcgtgtttg gtattctggg cgccttcgag gatgaacaca agttcaaatg
     1861 ggtgtacttt atcgctggat gtgtgatgca ggcagtcctg acatacggga tgtataacgc
     1921 cacttggaaa gacgatctga agaaaagccc cgagtaccat agctcctatg tcagtctgct
     1981 cgtcttcctg tcaatcctct gggtgtttta tcctgtcgtg tgggctttcg ggtctggtag
     2041 tggcgtgctg tccgtcgaca atgaggccat tctcatggga atcctggatg tgctcgctaa
     2101 gccactgttt ggaatggggt gcctcattgc ccatgagact atcttcaagt cctacccagc
     2161 gacacatgag ttacacatct ttggctccat caacggtgtg gactttgaca tggtgggtca
     2221 gggcaccggc aatccaaatg atggttatga ggagttaaac ctgaagtcca ccaagggtga
     2281 cctccagttc tccccctgga ttctggtccc tcatatcggg tatggcttcc atcagtacct
     2341 gccctaccct gacgggatgt cgcctttcca ggccgccatg gtagatggct ccggatacca
     2401 agtccatcgc acaatgcagt ttgaagatgg tgcctccctt actgttaact accgctacac
     2461 ctacgaggga agccacatca aaggagaggc ccaggtgaag gggactggtt tccctgctga
     2521 cggtcctgtg atgaccaact cgctgaccgc tgcggactgg tgcaggtcga agaagactta
     2581 ccccaacgac aaaaccatca tcagtacctt taagtggagt tacaccactg gaaatggcaa
     2641 gcgctacagg agcactgcgc ggaccaccta cacctttgcc aagccaatgg cggctaacta
     2701 tctgaagaac cagccgatgt acgtgttccg taagacggag ctcaagcact ccaagaccga
     2761 gctcaacttc aaggagtggc aaaaggcctt taccgatgtg atgggcatgg acgagctgta
     2821 caagaagagc aggatcacca gcgagggcga gtacatcccc ctggaccaga tcgacatcaa
     2881 cgtgttctgc tacgagaacg aggtgcaaag tcagcctatc ctgaacacaa aggaaatggc
     2941 tccacagtct aagcctcccg aagagcttga gatgtccagt atgccaagtc ccgtggctcc
     3001 cctccctgcc aggactgaag gagtgattga catgaggagt atgtcatcta ttgatagctt
     3061 catctcttgc gcaacagatt tccccgaggc tactcgattc taaaagctta tcgataatca
     3121 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3181 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3241 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3301 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3361 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3421 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3481 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3541 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3601 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3661 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct
     3721 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3781 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     3841 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     3901 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     3961 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     4021 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4081 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4141 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4201 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc
     4261 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4321 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4381 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4441 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4501 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4561 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4621 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4681 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4741 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4801 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4861 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     4921 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     4981 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5041 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5101 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5161 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5221 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5281 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5341 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5401 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5461 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5521 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5581 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5641 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5701 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5761 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5821 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5881 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5941 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6001 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6061 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6121 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6181 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6241 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6301 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6361 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6421 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6481 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6541 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6601 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6661 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6721 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6781 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6841 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6901 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6961 ggccttttgc tggccttttg ctcacatgt
//
