LOCUS       Exported                7010 bp ds-DNA     circular SYN 08-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CaMKII-VARNAM2-Kv2.1PR
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Pieribone Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 9, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(405..416)
                     /label=1mRub3_146
     primer_bind     1443..1480
                     /label=1CaM-Ac-KPN
     primer_bind     1456..1480
                     /label=1VA_NLS
     CDS             1469..3124
                     /codon_start=1
                     /label=VARNAM2-Kv2.1PR
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMNGERQVVYARYISWVLTTPLLLLDLIVMTKMGGVMI
                     SWVIGADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEY
                     HSSYVSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAH
                     ETIFKSILIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAF
                     DILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGE
                     LVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIALKVDGGGHLHCN
                     FVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELY
                     KKSRITSEGEYIPLDQIDINVFCYENEVQSQPILNTKEMAPQSKPPEELEMSSMPSPVA
                     PLPARTEGVIDMRSMSSIDSFISCATDFPEATRF"
     primer_bind     complement(1685..1708)
                     /label=2Ace_121
     primer_bind     1694..1742
                     /label=1Ace_DETC
     primer_bind     1694..1730
                     /label=1Ace_121A
     primer_bind     complement(1698..1720)
                     /label=2Ace_T125
     primer_bind     1711..1742
                     /label=1Ace_T125C
     primer_bind     1711..1742
                     /label=1Ace_125A
     primer_bind     complement(1727..1744)
                     /label=2_AceL93x
     primer_bind     complement(2075..2095)
                     /label=2Ace_A256
     primer_bind     2081..2118
                     /label=1Ace_A256M
     primer_bind     complement(2127..2149)
                     /label=2Ac_lnkr
     primer_bind     complement(2131..2152)
                     /label=2GLinker
     primer_bind     2135..2191
                     /label=1AcRub_228/7
     primer_bind     2135..2189
                     /label=1AcRub_228/6
     primer_bind     2135..2186
                     /label=1AcRub_d228/5
     primer_bind     2135..2184
                     /label=1AcRub_d228/4
     primer_bind     2135..2183
                     /label=1AcRub_d228/3
     primer_bind     2135..2179
                     /label=1AcRub_d228/2
     primer_bind     2135..2175
                     /label=1AcRub_d228/1
     primer_bind     2135..2173
                     /label=1AcRub_ins228
     primer_bind     2135..2173
                     /label=1ARb_LKins2
     primer_bind     complement(2135..2152)
                     /label=2Ace-mNeon_inv
     primer_bind     2138..2179
                     /label=1LinkerG_A
     primer_bind     2138..2179
                     /label=1LinkerG_B
     primer_bind     2138..2179
                     /label=1LinkerG_C
     primer_bind     2138..2179
                     /label=1LinkerG_D
     primer_bind     2138..2179
                     /label=1GLinkerY
     primer_bind     2138..2179
                     /label=1GLinkerH
     primer_bind     2138..2179
                     /label=1GLinkerV
     primer_bind     2141..2176
                     /label=1Ace-mRub3
     primer_bind     complement(2551..2587)
                     /label=2mRub3_146A
     primer_bind     complement(2557..2572)
                     /label=2Ruby-N147
     primer_bind     2558..2596
                     /label=1RubyN147_A
     primer_bind     2558..2596
                     /label=1RubyN147_B
     primer_bind     2558..2596
                     /label=1RubyN147_C
     primer_bind     2558..2596
                     /label=1RubyN147_D
     primer_bind     2573..2595
                     /label=1mRub3_146
     primer_bind     complement(2605..2641)
                     /label=2mRub3_164A
     primer_bind     2627..2645
                     /label=1mRub3_164
     primer_bind     complement(2827..2860)
                     /label=2Ace-mRub3
     primer_bind     complement(2827..2845)
                     /label=2mRUby2_SH1
     primer_bind     2846..2865
                     /label=1Ace-mNeon_inv
     primer_bind     complement(2908..2926)
                     /label=2TS/ER_inv
     primer_bind     complement(2909..2926)
                     /label=2VA_NLS
     primer_bind     2912..2950
                     /label=1Kv
     primer_bind     2912..2950
                     /label=Fragment.FOR
     primer_bind     2912..2950
                     /label=1Kv2.1px
     primer_bind     complement(3102..3124)
                     /label=2Kv2.1px
     primer_bind     complement(3102..3124)
                     
     primer_bind     complement(3102..3124)
                     /label=2Kv2.1px
     primer_bind     complement(3103..3140)
                     /label=2CaM-Ac-HINDIII
     primer_bind     complement(3103..3124)
                     /label=1DIO-ANv3-Kv2.1
     misc_feature    3137..3725
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3608..3619)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3757..4233
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4211..4220)
                     /label=2_AceL93x
     repeat_region   4273..4413
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4488..4943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5225..5329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5330..6190
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6361..6949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
      181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
      601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
      661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
      721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
      781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
      841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
      901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
      961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1441 ggggcagctc tagagcggta ccgccaccat ggctgacgtg gaaaccgaga ccggcatgat
     1501 tgcacagtgg attgtctttg ctattatggc tgctgctgct attgcttttg gagtggctgt
     1561 gcactttcgg ccttcagagc tgaagagcgc atactatatc aacattgcca tctgcactat
     1621 cgccgctacc gcttactatg caatggccgt gaactaccag gacctgacaa tgaatggtga
     1681 aaggcaggtg gtctacgcaa gatatattag ctgggtgctg accacaccac tgctcctgct
     1741 cgatctcatc gtcatgacca agatgggcgg agtgatgatt tcttgggtca tcggcgcaga
     1801 cattttcatg atcgtgtttg gtattctggg cgccttcgag gatgaacaca agttcaaatg
     1861 ggtgtacttt atcgctggat gtgtgatgca ggcagtcctg acatacggga tgtataacgc
     1921 cacttggaaa gacgatctga agaaaagccc cgagtaccat agctcctatg tcagtctgct
     1981 cgtcttcctg tcaatcctct gggtgtttta tcctgtcgtg tgggctttcg ggtctggtag
     2041 tggcgtgctg tccgtcgaca atgaggccat tctcatggga atcctggatg tgctcgctaa
     2101 gccactgttt ggaatggggt gcctcattgc ccatgagact atcttcaagt ccatcctgat
     2161 taaggaaaat atgcggatga aggtcgtgat ggaagggtct gtcaatgggc accagttcaa
     2221 gtgcaccgga gagggagagg gcaggccata cgagggcgtg cagacaatga ggatcaaagt
     2281 gatcgaggga ggaccactgc ctttcgcctt tgacatcctg gccaccagct tcatgtacgg
     2341 cagcaggacc ttcatcaagt atccagccga catccccgat ttctttaagc agagcttccc
     2401 cgagggcttt acctgggaga gggtgacaag atacgaggat ggcggcgtgg tgaccgtgac
     2461 acaggacacc tctctggagg atggcgagct ggtgtataac gtgaaggtga ggggcgtgaa
     2521 cttccctagc aatggcccag tgatgcagaa gaagaccaag ggctgggagc ccaatacaga
     2581 gatgatgtac cctgcagacg gaggcctgag gggatatacc gacatcgccc tgaaggtgga
     2641 tggaggagga cacctgcact gtaactttgt gaccacatac cgctccaaga agacagtggg
     2701 caatatcaag atgccaggag tgcacgccgt ggaccacagg ctggagcgca tcgaggagtc
     2761 tgataacgag acatatgtgg tgcagagaga ggtggccgtg gccaagtact ctaatctggg
     2821 cggcgggatg gacgagctgt ataagaagag caggatcacc agcgagggcg agtacatccc
     2881 cctggaccag atcgacatca acgtgttctg ctacgagaac gaggtgcaaa gtcagcctat
     2941 cctgaacaca aaggaaatgg ctccacagtc taagcctccc gaagagcttg agatgtccag
     3001 tatgccaagt cccgtggctc ccctccctgc caggactgaa ggagtgattg acatgaggag
     3061 tatgtcatct attgatagct tcatctcttg cgcaacagat ttccccgagg ctactcgatt
     3121 ctaaaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3181 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3241 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3301 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3361 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3421 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3481 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3541 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3601 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3661 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3721 cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc
     3781 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3841 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3901 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3961 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     4021 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     4081 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4141 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4201 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     4261 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4321 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4381 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     4441 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     4501 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4561 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4621 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4681 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4741 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4801 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     4861 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4921 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4981 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5041 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5101 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5161 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5221 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5281 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5341 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5401 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5461 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5521 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5581 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5641 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5701 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5761 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5821 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5881 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5941 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6001 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6061 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6121 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6181 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6241 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6301 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6481 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6961 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
