LOCUS       pJT244_lenti_pEF-dCas9 14880 bp ds-DNA     circular     11-MAR-2020
DEFINITION  .
KEYWORDS    "marker:BleoR, AmpR"
FEATURES             Location/Qualifiers
     enhancer        238..617
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        619..817
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1062..1187
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1680..1913
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        2687..3865
                     /label="EF-1α promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     intron          2918..3856
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     CDS             3881..3928
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             3929..8029
                     /label="dCas9"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             3929..9547
                     /label="Translation 3929-9547"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSDYKDHDGDYKDHDIDYKDDDDKGGSGSGSPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTGSGEGRGSLLTCGDVEENPGPMVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLNCTGSGATNFSLLKQAGDVEENPGPMAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAIVKDSDGQPTAVGIRELLPSGYVWEG*"
     primer          5530..5547
                     /label="QB5513"
                     /note="sequence: AAAGCCCGCCTTCCTGAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          6167..6185
                     /label="JT-890"
                     /note="sequence: GTGAAAGTGATGGGCCGGC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          6792..6811
                     /label="JT-891"
                     /note="sequence: TCACCCTGAAGTCCAAGCTG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          7342..7361
                     /label="JT-892"
                     /note="sequence: GGCCTATTCTGTGCTGGTGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             8084..8104
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    8120..8185
                     /label="3XFLAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             8120..8206
                     /label="Translation 8120-8206"
                     /translation="DYKDHDGDYKDHDIDYKDDDDKGGSGSGS"
     primer          8188..8206
                     /label="HTrec F bind"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    8189..8206
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    8207..8302
                     /label="RYBP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             8303..8308
                     /label="Translation 8303-8308"
                     /translation="GS"
     misc_feature    8303..8311
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(8303..8322)
                     /label="HTrec R bind site"
                     /note="sequence: cccctTccctcTCcagaTcc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             8309..8311
                     /label="Translation 8309-8311"
                     /translation="G"
     misc_feature    8312..8365
                     /label="T2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             8312..8365
                     /label="Translation 8312-8365"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer          complement(8347..8365)
                     /label="JT809"
                     /note="sequence: tgggccaggattctcctcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8366..9076
                     /label="mTagBFP2 (benchling human codon opt)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             8366..9076
                     /label="Translation 8366-9076"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN"
     primer          8722..8742
                     /label="QB2350"
                     /note="sequence: GATCTACAACGTCAAGATCAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    9083..9148
                     /label="P2A"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             9083..9148
                     /label="Translation 9083-9148"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             9149..9547
                     /label="BSD"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    9572..10160
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(10043..10054)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(10163..10179)
                     /label="KS primer"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     LTR             10685..10865
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    10897..11121
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      11167..11595
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        11609..11938
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      11789..11924
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        11986..12033
                     /label="EM7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             12052..12426
                     /label="BleoR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    12556..12677
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(12726..12742)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    12750..12766
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(12774..12780)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(12781..12798)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(12799..12804)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    12819..12840
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(13128..13716)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(13887..14678)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(14679..14747)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(14748..14852)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcTGCA AAGATGGATA AAGTTTTAAA CAGAGAGGAA
     2641 TCTTTGCAGC TAATGGACCT TCTAGGTCTT GAAAGGAGTG GGAATTGGCT CCGGTGCCCG
     2701 TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA
     2761 TTGAACCGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG
     2821 GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA
     2881 CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGGTA AGTGCCGTGT GTGGTTCCCG
     2941 CGGGCCTGGC CTCTTTACGG GTTATGGCCC TTGCGTGCCT TGAATTACTT CCACCTGGCT
     3001 GCAGTACGTG ATTCTTGATC CCGAGCTTCG GGTTGGAAGT GGGTGGGAGA GTTCGAGGCC
     3061 TTGCGCTTAA GGAGCCCCTT CGCCTCGTGC TTGAGTTGAG GCCTGGCCTG GGCGCTGGGG
     3121 CCGCCGCGTG CGAATCTGGT GGCACCTTCG CGCCTGTCTC GCTGCTTTCG ATAAGTCTCT
     3181 AGCCATTTAA AATTTTTGAT GACCTGCTGC GACGCTTTTT TTCTGGCAAG ATAGTCTTGT
     3241 AAATGCGGGC CAAGATCTGC ACACTGGTAT TTCGGTTTTT GGGGCCGCGG GCGGCGACGG
     3301 GGCCCGTGCG TCCCAGCGCA CATGTTCGGC GAGGCGGGGC CTGCGAGCGC GGCCACCGAG
     3361 AATCGGACGG GGGTAGTCTC AAGCTGGCCG GCCTGCTCTG GTGCCTGGCC TCGCGCCGCC
     3421 GTGTATCGCC CCGCCCTGGG CGGCAAGGCT GGCCCGGTCG GCACCAGTTG CGTGAGCGGA
     3481 AAGATGGCCG CTTCCCGGCC CTGCTGCAGG GAGCTCAAAA TGGAGGACGC GGCGCTCGGG
     3541 AGAGCGGGCG GGTGAGTCAC CCACACAAAG GAAAAGGGCC TTTCCGTCCT CAGCCGTCGC
     3601 TTCATGTGAC TCCACGGAGT ACCGGGCGCC GTCCAGGCAC CTCGATTAGT TCTCGAGCTT
     3661 TTGGAGTACG TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC
     3721 TGAGTGGGTG GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT
     3781 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT
     3841 TTTTCTTCCA TTTCAGGTGT CGTGAcGTAC GgccaccATG AAAAGGCCGG CGGCCACGAA
     3901 AAAGGCCGGC CAGGCAAAAA AGAAAAAGGA CAAGAAGTAC AGCATCGGCC TGGCCATCGG
     3961 CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT
     4021 CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT
     4081 GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA
     4141 CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA
     4201 GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA
     4261 GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA
     4321 CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG
     4381 GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG
     4441 CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA
     4501 CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT
     4561 GTCTGCCAGA CTGAGCAAGA GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGA
     4621 GAAGAAGAAT GGCCTGTTCG GCAACCTGAT TGCCCTGAGC CTGGGCCTGA CCCCCAACTT
     4681 CAAGAGCAAC TTCGACCTGG CCGAGGATGC CAAACTGCAG CTGAGCAAGG ACACCTACGA
     4741 CGACGACCTG GACAACCTGC TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTTCTGGC
     4801 CGCCAAGAAC CTGTCCGACG CCATCCTGCT GAGCGACATC CTGAGAGTGA ACACCGAGAT
     4861 CACCAAGGCC CCCCTGAGCG CCTCTATGAT CAAGAGATAC GACGAGCACC ACCAGGACCT
     4921 GACCCTGCTG AAAGCTCTCG TGCGGCAGCA GCTGCCTGAG AAGTACAAAG AGATTTTCTT
     4981 CGACCAGAGC AAGAACGGCT ACGCCGGCTA CATTGACGGC GGAGCCAGCC AGGAAGAGTT
     5041 CTACAAGTTC ATCAAGCCCA TCCTGGAAAA GATGGACGGC ACCGAGGAAC TGCTCGTGAA
     5101 GCTGAACAGA GAGGACCTGC TGCGGAAGCA GCGGACCTTC GACAACGGCA GCATCCCCCA
     5161 CCAGATCCAC CTGGGAGAGC TGCACGCCAT TCTGCGGCGG CAGGAAGATT TTTACCCATT
     5221 CCTGAAGGAC AACCGGGAAA AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT
     5281 GGGCCCTCTG GCCAGGGGAA ACAGCAGATT CGCCTGGATG ACCAGAAAGA GCGAGGAAAC
     5341 CATCACCCCC TGGAACTTCG AGGAAGTGGT GGACAAGGGC GCTTCCGCCC AGAGCTTCAT
     5401 CGAGCGGATG ACCAACTTCG ATAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG
     5461 CCTGCTGTAC GAGTACTTCA CCGTGTATAA CGAGCTGACC AAAGTGAAAT ACGTGACCGA
     5521 GGGAATGAGA AAGCCCGCCT TCCTGAGCGG CGAGCAGAAA AAGGCCATCG TGGACCTGCT
     5581 GTTCAAGACC AACCGGAAAG TGACCGTGAA GCAGCTGAAA GAGGACTACT TCAAGAAAAT
     5641 CGAGTGCTTC GACTCCGTGG AAATCTCCGG CGTGGAAGAT CGGTTCAACG CCTCCCTGGG
     5701 CACATACCAC GATCTGCTGA AAATTATCAA GGACAAGGAC TTCCTGGACA ATGAGGAAAA
     5761 CGAGGACATT CTGGAAGATA TCGTGCTGAC CCTGACACTG TTTGAGGACA GAGAGATGAT
     5821 CGAGGAACGG CTGAAAACCT ATGCCCACCT GTTCGACGAC AAAGTGATGA AGCAGCTGAA
     5881 GCGGCGGAGA TACACCGGCT GGGGCAGGCT GAGCCGGAAG CTGATCAACG GCATCCGGGA
     5941 CAAGCAGTCC GGCAAGACAA TCCTGGATTT CCTGAAGTCC GACGGCTTCG CCAACAGAAA
     6001 CTTCATGCAG CTGATCCACG ACGACAGCCT GACCTTTAAA GAGGACATCC AGAAAGCCCA
     6061 GGTGTCCGGC CAGGGCGATA GCCTGCACGA GCACATTGCC AATCTGGCCG GCAGCCCCGC
     6121 CATTAAGAAG GGCATCCTGC AGACAGTGAA GGTGGTGGAC GAGCTCGTGA AAGTGATGGG
     6181 CCGGCACAAG CCCGAGAACA TCGTGATCGA AATGGCCAGA GAGAACCAGA CCACCCAGAA
     6241 GGGACAGAAG AACAGCCGCG AGAGAATGAA GCGGATCGAA GAGGGCATCA AAGAGCTGGG
     6301 CAGCCAGATC CTGAAAGAAC ACCCCGTGGA AAACACCCAG CTGCAGAACG AGAAGCTGTA
     6361 CCTGTACTAC CTGCAGAATG GGCGGGATAT GTACGTGGAC CAGGAACTGG ACATCAACCG
     6421 GCTGTCCGAC TACGATGTGG ACCACATCGT GCCTCAGAGC TTTCTGAAGG ACGACTCCAT
     6481 CGACAACAAG GTGCTGACCA GAAGCGACAA GGCCCGGGGC AAGAGCGACA ACGTGCCCTC
     6541 CGAAGAGGTC GTGAAGAAGA TGAAGAACTA CTGGCGGCAG CTGCTGAACG CCAAGCTGAT
     6601 TACCCAGAGA AAGTTCGACA ATCTGACCAA GGCCGAGAGA GGCGGCCTGA GCGAACTGGA
     6661 TAAGGCCGGC TTCATCAAGA GACAGCTGGT GGAAACCCGG CAGATCACAA AGCACGTGGC
     6721 ACAGATCCTG GACTCCCGGA TGAACACTAA GTACGACGAG AATGACAAGC TGATCCGGGA
     6781 AGTGAAAGTG ATCACCCTGA AGTCCAAGCT GGTGTCCGAT TTCCGGAAGG ATTTCCAGTT
     6841 TTACAAAGTG CGCGAGATCA ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT
     6901 CGTGGGAACC GCCCTGATCA AAAAGTACCC TAAGCTGGAA AGCGAGTTCG TGTACGGCGA
     6961 CTACAAGGTG TACGACGTGC GGAAGATGAT CGCCAAGAGC GAGCAGGAAA TCGGCAAGGC
     7021 TACCGCCAAG TACTTCTTCT ACAGCAACAT CATGAACTTT TTCAAGACCG AGATTACCCT
     7081 GGCCAACGGC GAGATCCGGA AGCGGCCTCT GATCGAGACA AACGGCGAAA CCGGGGAGAT
     7141 CGTGTGGGAT AAGGGCCGGG ATTTTGCCAC CGTGCGGAAA GTGCTGAGCA TGCCCCAAGT
     7201 GAATATCGTG AAAAAGACCG AGGTGCAGAC AGGCGGCTTC AGCAAAGAGT CTATCCTGCC
     7261 CAAGAGGAAC AGCGATAAGC TGATCGCCAG AAAGAAGGAC TGGGACCCTA AGAAGTACGG
     7321 CGGCTTCGAC AGCCCCACCG TGGCCTATTC TGTGCTGGTG GTGGCCAAAG TGGAAAAGGG
     7381 CAAGTCCAAG AAACTGAAGA GTGTGAAAGA GCTGCTGGGG ATCACCATCA TGGAAAGAAG
     7441 CAGCTTCGAG AAGAATCCCA TCGACTTTCT GGAAGCCAAG GGCTACAAAG AAGTGAAAAA
     7501 GGACCTGATC ATCAAGCTGC CTAAGTACTC CCTGTTCGAG CTGGAAAACG GCCGGAAGAG
     7561 AATGCTGGCC TCTGCCGGCG AACTGCAGAA GGGAAACGAA CTGGCCCTGC CCTCCAAATA
     7621 TGTGAACTTC CTGTACCTGG CCAGCCACTA TGAGAAGCTG AAGGGCTCCC CCGAGGATAA
     7681 TGAGCAGAAA CAGCTGTTTG TGGAACAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA
     7741 GATCAGCGAG TTCTCCAAGA GAGTGATCCT GGCCGACGCT AATCTGGACA AAGTGCTGTC
     7801 CGCCTACAAC AAGCACCGGG ATAAGCCCAT CAGAGAGCAG GCCGAGAATA TCATCCACCT
     7861 GTTTACCCTG ACCAATCTGG GAGCCCCTGC CGCCTTCAAG TACTTTGACA CCACCATCGA
     7921 CCGGAAGAGG TACACCAGCA CCAAAGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT
     7981 CACCGGCCTG TACGAGACAC GGATCGACCT GTCTCAGCTG GGAGGCGACa gcgctGGAGG
     8041 AGGTGGAAGC GGAGGAGGAG GAAGCGGAGG AGGAGGTAGC ggacctaaga aaaagaggaa
     8101 ggtggcggcc gctggatccg actacaaaga ccatgacggt gattataaag atcatgacat
     8161 cgactacaag gatgacgatg acaagGGTgg ATCCggaTCA ggctccCCTA GGTTGAAGAA
     8221 TGTTGATAGA TCCACCGCTC AACAGTTGGC CGTGACTGTG GGAAATGTCA CAGTCATTAT
     8281 CACAGATTTT AAGGAAAAAA CCggAtctgG AgagggAagg ggAagcctcc taacttgcgg
     8341 agaTgtcgag gagaatcctg gcccaATGGT TAGTAAGGGA GAGGAGTTGA TAAAAGAGAA
     8401 TATGCACATG AAACTGTATA TGGAAGGGAC AGTGGACAAC CATCACTTCA AGTGCACCTC
     8461 TGAGGGAGAA GGAAAGCCAT ACGAAGGCAC CCAGACTATG CGAATTAAGG TCGTGGAGGG
     8521 AGGGCCTCTC CCCTTCGCCT TCGATATTCT TGCAACATCT TTCCTCTACG GGAGCAAAAC
     8581 CTTCATCAAT CACACACAAG GCATACCTGA CTTTTTTAAG CAGAGTTTTC CAGAGGGCTT
     8641 TACCTGGGAG CGTGTGACTA CATACGAAGA TGGGGGTGTG CTGACGGCTA CACAGGATAC
     8701 TTCACTTCAG GACGGTTGTC TGATCTACAA CGTCAAGATC AGGGGGGTCA ATTTCACCTC
     8761 GAATGGACCA GTAATGCAAA AAAAGACCCT GGGATGGGAA GCTTTTACTG AAACTCTCTA
     8821 TCCCGCCGAC GGCGGCCTCG AAGGCAGAAA CGATATGGCT TTGAAGCTGG TTGGCGGGTC
     8881 CCATCTAATT GCCAATGCCA AAACCACATA TAGGAGCAAA AAGCCTGCAA AGAACCTGAA
     8941 GATGCCGGGT GTGTACTATG TTGACTATAG ATTAGAGCGG ATCAAAGAGG CAAATAACGA
     9001 AACGTACGTG GAGCAGCACG AGGTAGCCGT GGCGCGCTAC TGCGATTTAC CCTCCAAACT
     9061 GGGCCATAAA CTGAACtgta caGGAAGCGG AGCTACTAAC TTCAGCCTGC TGAAGCAGGC
     9121 TGGcGACGTG GAaGAaAACC CaGGACCTat ggccaagcct ttgtctcaag aagaatccac
     9181 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     9241 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     9301 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     9361 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     9421 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     9481 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     9541 gggctaagaa ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga
     9601 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     9661 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     9721 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     9781 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     9841 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     9901 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     9961 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    10021 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    10081 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
    10141 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
    10201 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
    10261 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
    10321 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
    10381 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
    10441 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
    10501 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
    10561 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
    10621 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
    10681 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    10741 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10801 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10861 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
    10921 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
    10981 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
    11041 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
    11101 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
    11161 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    11221 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    11281 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    11341 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    11401 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    11461 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    11521 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    11581 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
    11641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
    11701 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    11761 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    11821 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
    11881 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    11941 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
    12001 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
    12061 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
    12121 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
    12181 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
    12241 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
    12301 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
    12361 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
    12421 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    12481 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    12541 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    12601 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    12661 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
    12721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    12781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    12841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    12901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    12961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    13021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    13081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    13141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    13201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    13261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    13321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    13381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    13441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    13501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    13561 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    13621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    13681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    13741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    13801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    13861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    13921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    13981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    14041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    14101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    14161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    14221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    14281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    14341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    14401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    14461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    14521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    14581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    14641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    14701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    14761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    14821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//