LOCUS       pKL038_full             8824 bp ds-DNA     circular     16-JUN-2022
DEFINITION  .
KEYWORDS    "marker:AmpR, PuroR, DR"
FEATURES             Location/Qualifiers
     CDS             complement(260..271)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: Factor Xa recognition and cleavage site transl_table: 1 translation: IEGR"
     primer_bind     complement(380..396)
                     /label="KS primer"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     LTR             906..1086
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(1148..1736)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1907..2698)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(2699..2767)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer          2731..2754
                     /label="QB1023"
                     /note="sequence: agggaataagggcgacacggaaat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        complement(2768..2872)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     enhancer        3147..3526
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     primer          complement(3214..3234)
                     /label="QB2743"
                     /note="sequence: agttatgtaacgcggaactcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        3528..3726
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     primer          complement(3600..3618)
                     /label="QB2346"
                     /note="sequence: ctcccattgacgtcaatgg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     LTR             3744..3924
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3971..4096
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: packaging signal of human immunodeficiency virus type 1"
     misc_feature    4595..4828
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm."
     CDS             5013..5057
                     /label="gp41 peptide"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: recognized by the 2H10 single-chain llama nanobody product: antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013) transl_table: 1 translation: KNEQELLELDKWASL"
     misc_feature    5355..5472
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: central polypurine tract and central termination sequence of HIV-1"
     promoter        5545..5785
                     /label="U6 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: RNA polymerase III promoter for human U6 snRNA"
     primer          5583..5604
                     /label="QB5851"
                     /note="sequence: acgatacaaggctgttagagag"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5796..5814
                     /label="DR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5836..5854
                     /label="DR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        5965..6176
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: core promoter for human elongation factor EF-1α"
     primer          complement(6016..6036)
                     /label="QB2749"
                     /note="sequence: TCTAGGCACCGGTTCAATTGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     intron          6177..7115
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
                     /note="note: intron upstream of the start codon of human EF-1α"
     CDS             7131..7727
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: pac from Streptomyces alboniger note: confers resistance to puromycin product: puromycin N-acetyltransferase transl_table: 1 translation: MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             7728..7781
                     /label="T2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. product: 2A peptide from Thosea asigna virus capsid protein protein_id: transl_table: 1 translation: EGRGSLLTCGDVEENPGP"
     regulatory      7788..7797
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     CDS             7794..8504
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="codon_start: 1 note: mammalian codon-optimized product: monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004) transl_table: 1 translation: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK*"
     protein_bind    8524..8557
                     /label="loxP"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cre recombinase note: Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
     misc_feature    8613..377
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: woodchuck hepatitis virus posttranscriptional regulatory element"
ORIGIN
        1 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
       61 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
      121 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
      181 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
      241 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
      301 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
      361 ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag
      421 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
      481 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
      541 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
      601 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
      661 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
      721 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
      781 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
      841 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
      901 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      961 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     1021 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     1081 ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1561 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1801 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1861 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1921 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1981 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2041 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2101 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2161 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2221 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2281 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2341 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2401 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2461 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2521 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2581 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2641 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2701 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2761 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2821 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2881 cccgaaaagt gccacctgac gcttaagcgg tcgacggatc gggagatctc ccgatcccct
     2941 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     3001 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     3061 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     3121 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     3181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3541 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3601 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3661 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3721 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3781 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3841 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3901 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     3961 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     4021 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     4081 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     4141 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     4201 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     4261 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     4321 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     4381 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     4441 ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     4501 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     4561 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     4621 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     4681 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     4741 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     4801 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     4861 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     4921 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     4981 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     5041 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     5101 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     5161 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     5221 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     5281 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     5341 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     5401 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     5461 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc
     5521 ccgctctaga aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat
     5581 atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata
     5641 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     5701 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     5761 gctttatata tcttgtggaa aggacgaaac accgtAATTT CTACTCTTGT AGATGGAGAC
     5821 GATTAATGCG TCTCCAATTT CTACTCTTGT AGATTTTTTT Taagcttggg ccgctcgagg
     5881 tacctctcta catatgacat gtgagcggat ccattaggcg gccgcgtgga taaccgtatt
     5941 accgccatgc atGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
     6001 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
     6061 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA
     6121 GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA
     6181 GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT
     6241 GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG
     6301 GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG
     6361 CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG
     6421 CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT
     6481 TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG
     6541 GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC
     6601 TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG
     6661 TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG
     6721 CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT
     6781 GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT
     6841 TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC
     6901 TCGATTAGTT CTCGcGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG
     6961 CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA
     7021 TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC
     7081 AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAgCTAGC atgaccgagt
     7141 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccGTA CGCACCCTCG
     7201 CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG
     7261 AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG
     7321 TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG
     7381 CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG
     7441 CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT
     7501 TCCTGGCCAC CGTCGGCGTA TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG
     7561 TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG
     7621 CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG
     7681 TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCGAG GGCAGAGGAA
     7741 GTCTGCTAAC ATGCGGTGAC GTCGAGGAGA ATCCTGGCCC Accggtcgcc accatggtga
     7801 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     7861 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     7921 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     7981 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     8041 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     8101 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     8161 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     8221 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     8281 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     8341 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     8401 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     8461 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagaattc gtcgagggac
     8521 ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg ttccggttcc
     8581 actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     8641 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8701 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8761 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8821 tgtg
//