LOCUS       pAR045                 12371 bp ds-DNA     circular     13-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    51..56
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          354..367
                     /label="HTrec F"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          354..367
                     /label="oAR161"
                     /note="sequence: TggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        complement(505..510)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             764..944
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(947..960)
                     /label="oAR161"
                     /note="sequence: TggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(947..960)
                     /label="HTrec F"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    991..1116
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1008..1021)
                     /label="blast forward primer"
                     /note="sequence: CTTCCATTTCAGGTGTCGTGAgCTAGCCCA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1011..1023)
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1034..1045
                     /label="oAR158"
                     /note="sequence: CGTCGCGGGTGGCGAGGCGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1037..1051)
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1609..1842
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2616..3793
                     /label="EF1a promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(2710..2715)
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(3066..3071)
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3125..3142)
                     /label="blast reverse primer"
                     /note="sequence: GATTCTCCTCGACGTCACCGCATGTTAGCA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(3166..3171)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3210..3226
                     /label="oAR157"
                     /note="sequence: AACGCGCCGAGGGCCGCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3272..3284
                     /label="oAR157"
                     /note="sequence: AACGCGCCGAGGGCCGCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3280..3285
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3300..3318
                     /label="QB1511"
                     /note="sequence: GGTAGTCTCAAGCTGGCCG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3329..3341)
                     /label="oAR157"
                     /note="sequence: AACGCGCCGAGGGCCGCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(3329..3344)
                     /label="oAR158"
                     /note="sequence: CGTCGCGGGTGGCGAGGCGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3616..5152
                     /label="gAR006"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3809..3814
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3818..3838
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3863..7783
                     /label="dEnAsCas12a"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4198..4213
                     /label="QB3001"
                     /note="sequence: gcacccagaccgccaagctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4207..4221)
                     /label="qb0420"
                     /note="sequence: aagtgtagcggtcacgctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4303..4317)
                     /label="qb0420"
                     /note="sequence: aagtgtagcggtcacgctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4351..4368
                     /label="oAR160"
                     /note="sequence: TCCACGTCTCCAGCCTGCTT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        4355..4360
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(4897..4910)
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4902..4914
                     /label="oAR159"
                     /note="sequence: ATCCGCTAGAGATCCGCGGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4982..4997)
                     /label="oAR115"
                     /note="sequence: tcagagatggggatgctgtt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5121..6689
                     /label="gAR007"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(5263..5277)
                     /label="oAR161"
                     /note="sequence: TggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(5263..5277)
                     /label="HTrec F"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(5270..5284)
                     /label="QB3001"
                     /note="sequence: gcacccagaccgccaagctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5423..5428
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(5483..5497)
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          6069..6082
                     /label="QB3001"
                     /note="sequence: gcacccagaccgccaagctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(6104..6119)
                     /label="Blast Forward"
                     /note="sequence: CTTCCATTTCAGGTGTCGTGAgCTAGCCCACCATGGCCAAGCCTTT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          6113..6127
                     /label="oAR157"
                     /note="sequence: AACGCGCCGAGGGCCGCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6120..6125
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(6403..6416)
                     /label="qb0420"
                     /note="sequence: aagtgtagcggtcacgctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    6657..8695
                     /label="gAR008"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(6715..6720)
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          7417..7432
                     /label="oAR158"
                     /note="sequence: CGTCGCGGGTGGCGAGGCGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          7472..7491
                     /label="oAR094"
                     /note="sequence: ctggagaatgacgattctca"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(7507..7512)
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(7603..7617)
                     /label="oAR158"
                     /note="sequence: CGTCGCGGGTGGCGAGGCGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(7683..7696)
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             7784..7831
                     /label="nucleoplasmin NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    7838..7918
                     /label="3X HA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             7919..7939
                     /label="Translation 7919-7939"
                     /translation="GGSGSGS"
     primer          7921..7939
                     /label="oAR161"
                     /note="sequence: TggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          7921..7939
                     /label="HTrec F"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    7922..7939
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(7922..7940)
                     /label="HTrec F"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(7922..7940)
                     /label="oAR161"
                     /note="sequence: TggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          7934..7948
                     /label="oAR160"
                     /note="sequence: TCCACGTCTCCAGCCTGCTT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    7940..8503
                     /label="QNZF_gJT171"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(8003..8017)
                     /label="oAR158"
                     /note="sequence: CGTCGCGGGTGGCGAGGCGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             8504..8509
                     /label="Translation 8504-8509"
                     /translation="GS"
     misc_feature    8504..8512
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          8506..8534
                     /label="mCherry1 forward primer"
                     /note="sequence: ATCGGGAGAGGGCAGAGGAAGTCTGCTAA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             8510..8512
                     /label="Translation 8510-8512"
                     /translation="G"
     misc_feature    8513..8566
                     /label="T2A"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          8529..8558
                     /label="mCherry forward primer"
                     /note="sequence: TGCTAACATGCGGTGACGTCGAGGAGAATC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(8529..8558)
                     /label="blast reverse primer"
                     /note="sequence: GATTCTCCTCGACGTCACCGCATGTTAGCA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    8573..8971
                     /label="BSD"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(8633..8652)
                     /label="oAR115"
                     /note="sequence: tcagagatggggatgctgtt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8653..8653
                     /label="silent mutation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          8771..8785
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(8975..9008)
                     /label="oAR97"
                     /note="sequence: cacaaattttgtaatccagaggttgattgtcgac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        9032..9038
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(9034..9054)
                     /label="QB0112"
                     /note="sequence: catagcgtaaaaggagcaaca"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(9196..9209)
                     /label="blast forward primer"
                     /note="sequence: CTTCCATTTCAGGTGTCGTGAgCTAGCCCA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(9226..9238)
                     /label="oAR158"
                     /note="sequence: CGTCGCGGGTGGCGAGGCGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    9233..9238
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    9410..9415
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(9428..9442)
                     /label="oAR157"
                     /note="sequence: AACGCGCCGAGGGCCGCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(9452..9463)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     LTR             9696..9876
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    9948..10069
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(9957..9980)
                     /label="QB1734"
                     /note="sequence: tttgtaaccattataagctgcaat"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(10277..10293)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     protein_bind    10301..10317
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(10325..10331)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(10332..10349)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(10350..10355)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    10370..10391
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     primer          10539..10552
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(10547..10561)
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      complement(10679..11267)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(10735..10740)
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(11092..11097)
                     /label="kozak"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          11200..11213
                     /label="oAR095(partial)"
                     /note="sequence: gccgctgccgctagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(11438..12229)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          11763..11780
                     /label="QB1511"
                     /note="sequence: GGTAGTCTCAAGCTGGCCG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          11795..11810
                     /label="oAR161"
                     /note="sequence: TggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          11795..11810
                     /label="HTrec F"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(11847..11862)
                     /label="blast reverse primer"
                     /note="sequence: GATTCTCCTCGACGTCACCGCATGTTAGCA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(12230..12298)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(12299..32)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
       61 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
      121 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
      181 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
      241 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
      301 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
      361 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      421 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      481 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct gacgcgtgta
      541 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
      601 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
      661 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
      721 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
      781 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      841 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      901 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      961 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     1021 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     1081 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     1141 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1201 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1261 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1321 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1381 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1441 accgcacagc aagcggccac tgatcttcag acctggagga ggagatatga gggacaattg
     1501 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     1561 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     1621 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     1681 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     1741 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     1801 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     1861 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     1921 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     1981 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     2041 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     2101 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     2161 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     2221 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     2281 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gttgcttgga
     2341 tcgagcaatt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2401 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2461 atttctgcgt tgttgtcggt gctcgttctc tgctcttcac gctactgaat tcatcaccgg
     2521 ttcttcgaat ctagttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2581 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2641 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     2701 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     2761 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     2821 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     2881 tctttacggg ttatggccct tgcgtgcctt gaattacttc cactggctgc agtacgtgat
     2941 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     3001 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     3061 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     3121 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     3181 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     3241 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     3301 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     3361 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     3421 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     3481 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     3541 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     3601 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     3661 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     3721 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     3781 tcaggtgtcg tgaGTGTCGT GAgctagcgc caccATGcca aagaagaagc ggaaggtcgg
     3841 tatccacgga gtcccagcag ccatgacaca gttcgagggc tttaccaacc tgtatcaggt
     3901 gagcaagaca ctgcggtttg agctgatccc acagggcaag accctgaagc acatccagga
     3961 gcagggcttc atcgaggagg acaaggcccg caatgatcac tacaaggagc tgaagcccat
     4021 catcgatcgg atctacaaga cctatgccga ccagtgcctg cagctggtgc agctggattg
     4081 ggagaacctg agcgccgcca tcgactccta tagaaaggag aaaaccgagg agacaaggaa
     4141 cgccctgatc gaggagcagg ccacatatcg caatgccatc cacgactact tcatcggccg
     4201 gacagacaac ctgaccgatg ccatcaataa gagacacgcc gagatctaca agggcctgtt
     4261 caaggccgag ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga ccacaaccga
     4321 gcacgagaac gccctgctgc ggagcttcga caagtttaca acctacttct ccggctttta
     4381 tagaaacagg aagaacgtgt tcagcgccga ggatatcagc acagccatcc cacaccgcat
     4441 cgtgcaggac aacttcccca agtttaagga gaattgtcac atcttcacac gcctgatcac
     4501 cgccgtgccc agcctgcggg agcactttga gaacgtgaag aaggccatcg gcatcttcgt
     4561 gagcacctcc atcgaggagg tgttttcctt ccctttttat aaccagctgc tgacacagac
     4621 ccagatcgac ctgtataacc agctgctggg aggaatctct cgggaggcag gcaccgagaa
     4681 gatcaagggc ctgaacgagg tgctgaatct ggccatccag aagaatgatg agacagccca
     4741 catcatcgcc tccctgccac acagattcat ccccctgttt aagcagatcc tgtccgatag
     4801 gaacaccctg tctttcatcc tggaggagtt taagagcgac gaggaagtga tccagtcctt
     4861 ctgcaagtac aagacactgc tgagaaacga gaacgtgctg gagacagccg aggccctgtt
     4921 taacgagctg aacagcatcg acctgacaca catcttcatc agccacaaga agctggagac
     4981 aatcagcagc gccctgtgcg accactggga tacactgagg aatgccctgt atgagcggag
     5041 aatctccgag ctgacaggca agatcaccaa gtctgccaag gagaaggtgc agcgcagcct
     5101 gaagcacgag gatatcaacc tgcaggagat catctctgcc gcaggcaagg agctgagcga
     5161 ggccttcaag cagaaaacca gcgagatcct gtcccacgca cacgccgccc tggatcagcc
     5221 actgcctaca accctgaaga agcaggagga gaaggagatc ctgaagtctc agctggacag
     5281 cctgctgggc ctgtaccacc tgctggactg gtttgccgtg gatgagtcca acgaggtgga
     5341 ccccgagttc tctgcccggc tgaccggcat caagctggag atggagcctt ctctgagctt
     5401 ctacaacaag gccagaaatt atgccaccaa gaagccctac tccgtggaga agttcaagct
     5461 gaactttcag atgcctacac tggccagagg ctgggacgtg aatagagaga agaacaatgg
     5521 cgccatcctg tttgtgaaga acggcctgta ctatctgggc atcatgccaa agcagaaggg
     5581 caggtataag gccctgagct tcgagcccac agagaaaacc agcgagggct ttgataagat
     5641 gtactatgac tacttccctg atgccgccaa gatgatccca aagtgcagca cccagctgaa
     5701 ggccgtgaca gcccactttc agacccacac aacccccatc ctgctgtcca acaatttcat
     5761 cgagcctctg gagatcacaa aggagatcta cgacctgaac aatcctgaga aggagccaaa
     5821 gaagtttcag acagcctacg ccaagaaaac cggcgaccag aagggctaca gagaggccct
     5881 gtgcaagtgg atcgacttca caagggattt tctgtccaag tataccaaga caacctctat
     5941 cgatctgtct agcctgcggc catcctctca gtataaggac ctgggcgagt actatgccga
     6001 gctgaatccc ctgctgtacc acatcagctt ccagagaatc gccgagaagg agatcatgga
     6061 tgccgtggag acaggcaagc tgtacctgtt ccagatctat aacaaggact ttgccaaggg
     6121 ccaccacggc aagcctaatc tgcacacact gtattggacc ggcctgtttt ctccagagaa
     6181 cctggccaag acaagcatca agctgaatgg ccaggccgag ctgttctacc gccctaagtc
     6241 caggatgaag aggatggcac accggctggg agagaagatg ctgaacaaga agctgaagga
     6301 tcagaaaacc ccaatccccg acaccctgta ccaggagctg tacgactatg tgaatcacag
     6361 actgtcccac gacctgtctg atgaggccag ggccctgctg cccaacgtga tcaccaagga
     6421 ggtgtctcac gagatcatca aggataggcg ctttaccagc gacaagttct ttttccacgt
     6481 gcctatcaca ctgaactatc aggccgccaa ttccccatct aagttcaacc agagggtgaa
     6541 tgcctacctg aaggagcacc ccgagacacc tatcatcggc atcgcccggg gcgagagaaa
     6601 cctgatctat atcacagtga tcgactccac cggcaagatc ctggagcagc ggagcctgaa
     6661 caccatccag cagtttgatt accagaagaa gctggacaac agggagaagg agagggtggc
     6721 agcaaggcag gcctggtctg tggtgggcac aatcaaggat ctgaagcagg gctatctgag
     6781 ccaggtcatc cacgagatcg tggacctgat gatccactac caggccgtgg tggtgctgga
     6841 gaacctgaat ttcggcttta agagcaagag gaccggcatc gccgagaagg ccgtgtacca
     6901 gcagttcgag aagatgctga tcgataagct gaattgcctg gtgctgaagg actatccagc
     6961 agagaaagtg ggaggcgtgc tgaacccata ccagctgaca gaccagttca cctcctttgc
     7021 caagatgggc acccagtctg gcttcctgtt ttacgtgcct gccccatata catctaagat
     7081 cgatcccctg accggcttcg tggacccctt cgtgtggaaa accatcaaga atcacgagag
     7141 ccgcaagcac ttcctggagg gcttcgactt tctgcactac gacgtgaaaa ccggcgactt
     7201 catcctgcac tttaagatga acagaaatct gtccttccag aggggcctgc ccggctttat
     7261 gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag tttgacgcca agggcacccc
     7321 tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat cacagattca ccggcagata
     7381 ccgggacctg tatcctgcca acgagctgat cgccctgctg gaggagaagg gcatcgtgtt
     7441 cagggatggc tccaacatcc tgccaaagct gctggagaat gacgattctc acgccatcga
     7501 cacgatggtg gccctgatcc gcagcgtgct gcagatgcgg aactccaatg ccgccacagg
     7561 cgaggactat atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg actcccggtt
     7621 tcagaaccca gagtggccaa tggacgccga tgccaatggc gcctaccaca tcgccctgaa
     7681 gggccagctg ctgctgaatc acctgaagga gagcaaggat ctgaagctgc agaacggcat
     7741 ctccaatcag gactggctgg cctacatcca ggagctgcgc aacaaaaggc cggcggccac
     7801 gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac ccatacgatg ttccagatta
     7861 cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg actatgccGG
     7921 TggATCCgga TCAggctccA GTCCGTTTTC CCCTGTTCAA CTCCATCAAC TTCGGGCGCA
     7981 AATTCTTGCA TATAAAATGC TCGCTCGTGG TCAACCGTTG CCAGAAACTC TTCAACTTGC
     8041 AGTCCAGGGT GGCGGTGGTG GCTCCGGCGA AGGTCAATCC GATGAACGCG CACTTCTTGA
     8101 TCAACTTCAT ACATTGCTTA GTAATACAGA TGCAACTGGT CTCGAAGAAA TTGATCGGGC
     8161 GCTCGGGATA CCTGAACTTG TCAATCAAGG TCAAGCACTT GAACCTAAAC AAGGCGGCGG
     8221 TGGATCAGGT TTTGTTACAT TGAAAGATGT AGGGATGGAT TTTACACTCG GGGATTGGGA
     8281 ACAATTGGGG CTCGAACAAG GGGATACGTT TTGGGATACT GCTCTCGATA ATTGTCAAGA
     8341 TCTCTTTCTC CTCGATCCAC CTGGCGGCGG TGGGTCTGGC CATGAGAAAT TTCCGTCTGA
     8401 TCTCGATTTG GATATGTTTA ATGGGTCACT CGAATGTGAT ATGGAATCAA TTATTCGCAG
     8461 TGAACTCATG GATGCAGATG GACTCGATTT TAATTTTGAT TCAggAtctg GAgagggcag
     8521 aggaagcctt ctaacatgcg gtgacgtgga ggagaatccc ggcccttccg ggatggccaa
     8581 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     8641 ccccatctct gaggactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     8701 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     8761 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     8821 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     8881 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     8941 gctgccctct ggttatgtgt gggagggcta ataagtcgac aatcaacctc tggattacaa
     9001 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     9061 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     9121 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     9181 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     9241 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     9301 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     9361 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     9421 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     9481 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag
     9541 tcggatctct ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     9601 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9661 cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9901 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     9961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    10021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
    10081 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    10141 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10201 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga
    10261 ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    10321 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    10381 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    10441 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    10501 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    10561 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    10621 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10681 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10741 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10801 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10861 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10921 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10981 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11041 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11101 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11161 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    11221 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    11281 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    11341 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    11401 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    11461 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    11521 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    11581 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    11641 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    11701 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    11761 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11821 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11881 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11941 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    12001 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    12061 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    12121 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    12181 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    12241 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    12301 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    12361 tatttgaatg t
//