LOCUS       attB-p7-attP_flagged_YFP_Reporter_-_Victoria's_Archive_(pVHct1Y)        4656 bp    DNA     circular UNA 02-DEC-2022
DEFINITION  Bxb1 Target with 3x-FLAG VenusYFP reporter.
ACCESSION   urn.local.maksmac.x-fmbyrbx
VERSION     urn.local.maksmac.x-fmbyrbx
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    176..>180
                     /created_by="User"
                     /label="BBa prefix"
     misc            181..230
                     /created_by="User"
                     /label="Bxb1 attB"
     primer_bind     181..198
                     /%GC="72.2"
                     /Hairpin_Tm="46.2"
                     /Self_Dimer_Tm="28.6"
                     /Tm="65.3"
                     /annotation_group="333851753428066ae03b1f-de04-44b3-9944-d
                     cca9c26b8c5"
                     /created_by="primer3"
                     /Sequence="TCGGCCGGCTTGTCGACG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pVHct1Y"
                     /label="Fwd Bxb1 AttB TT"
     primer_bind     complement(213..230)
                     /%GC="66.7"
                     /Hairpin_Tm="33.3"
                     /Self_Dimer_Tm="10.2"
                     /Tm="60.0"
                     /annotation_group="33442654205030b71aa0da-7940-4a23-857d-9
                     dc8f9148692"
                     /created_by="primer3"
                     /Sequence="GCCCGGATGATCCTGACG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pVHct1Y"
                     /label="Rev Bxb1 AttB TT"
     promoter        complement(237..283)
                     /created_by="User"
                     /label="P7 promoter"
     misc            complement(284..336)
                     /created_by="User"
                     /label="Bxb1 AttP"
     primer_bind     284..303
                     /%GC="55.0"
                     /Hairpin_Tm="46.0"
                     /Self_Dimer_Tm="11.0"
                     /Tm="58.5"
                     /annotation_group="336549394999321e1e69ab-5803-458a-a5f1-e
                     ac417a17f82"
                     /created_by="primer3"
                     /Sequence="GGGTTTGTACCGTACACCAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pVHct1Y"
                     /label="Fwd Bxb1 AttP TC"
     primer_bind     complement(316..336)
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.7"
                     /annotation_group="336834832270156a2f7ee7-f04a-4f1f-890a-1
                     adca3610097"
                     /created_by="primer3"
                     /Sequence="TCGTGGTTTGTCTGGTCAACC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pVHct1Y"
                     /label="Rev Bxb1 AttP TC"
     misc_feature    337..411
                     /created_by="User"
                     /modified_by="User"
                     /label="Ribo J"
     misc            412..499
                     /created_by="User"
                     /label="BCD2_apFAB682"
     primer_bind     480..519
                     /created_by="User"
                     /Sequence="GCTAAGGAGGTTTTCTAATGATGGACTACAAGGACCATGA"
                     /modified_by="User"
                     /label="pVH003:YFP_F"
     misc_feature    complement(480..499)
                     /Sequence="CATTAGAAAACCTCCTTAGC"
                     /Hairpin_Tm="None"
                     /Tm="51.2"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /modified_by="User"
                     /label="pVHctbb_R"
                     /note="Geneious type: primer_bind_reverse"
     gene            500..1321
                     /created_by="User"
                     /modified_by="User"
                     /label="Venus YFP"
     primer_bind     500..519
                     /Sequence="ATGGACTACAAGGACCATGA"
                     /Hairpin_Tm="37.4"
                     /Tm="55.5"
                     /Self_Dimer_Tm="7.0"
                     /%GC="45.0"
                     /created_by="primer3"
                     /label="pVH003:YFP_Fbind"
     misc_feature    503..568
                     /created_by="User"
                     /modified_by="User"
                     /label="3x FLAG tag"
     primer_bind     925..940
                     /Sequence="CGAAGGTGACACACTT"
                     /Hairpin_Tm="34.4"
                     /Tm="50.4"
                     /Self_Dimer_Tm="8.5"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="YFP_seq_F"
     misc_feature    complement(962..977)
                     /Sequence="CTTTGAAATCAATCCC"
                     /Hairpin_Tm="35.6"
                     /Tm="43.3"
                     /Self_Dimer_Tm="None"
                     /%GC="37.5"
                     /created_by="primer3"
                     /label="YFP_seq_R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1299..1338)
                     /Sequence="GATGCCTGGCTCTAGTATCATCATGCTGCGGTACCAGAAC"
                     /created_by="User"
                     /label="pVH003:YFP_R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1299..1314)
                     /Sequence="GCTGCGGTACCAGAAC"
                     /Hairpin_Tm="None"
                     /Tm="54.6"
                     /Self_Dimer_Tm="8.0"
                     /%GC="62.5"
                     /created_by="primer3"
                     /label="pVH003:YFP_Rbind"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1315..1341
                     /Sequence="ATGATGATACTAGAGCCAGGCATCAAA"
                     /Hairpin_Tm="None"
                     /Tm="62.1"
                     /Self_Dimer_Tm="12.3"
                     /%GC="40.7"
                     /created_by="primer3"
                     /label="pVHctbb_F"
     terminator      1330..1458
                     /created_by="User"
                     /label="B0015"
     misc_feature    1459..1479
                     /created_by="User"
                     /label="BBa Suffix"
     misc_feature    complement(1574..1593)
                     /created_by="User"
                     /modified_by="User"
                     /label="VR primer"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1642..3678
                     /created_by="User"
                     /label="pSC101"
                     /note="Geneious type: origin of replication"
     resistance      3687..4629
                     /created_by="User"
                     /label="AmpR"
     primer_bind     4634..2
                     /Sequence="AGCTCCGGCAAAAAAACGGGCAAGG"
                     /Hairpin_Tm="47.1"
                     /Tm="68.8"
                     /Self_Dimer_Tm="14.7"
                     /%GC="56.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="pVHed01_seq_fwd1"
ORIGIN      
        1 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct
       61 gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga
      121 aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt
      181 tcggccggct tgtcgacgac ggcggtctcc gtcgtcagga tcatccgggc aagctttcca
      241 cacattatag gtacaaaaag atgcgaaagc aaataaattt tttgggtttg taccgtacac
      301 cactgagacc gcggtggttg accagacaaa ccacgaagct gtcaccggat gtgctttccg
      361 gtctgatgag tccgtgagga cgaaacagcc tctacaaata attttgttta agggcccaag
      421 ttcacttaaa aaggagatca acaatgaaag caattttcgt actgaaacat cttaatcatg
      481 ctaaggaggt tttctaatga tggactacaa ggaccatgac ggtgattaca aggatcatga
      541 catcgactac aaggatgacg atgacaaggc aactagcggc atggttagta aaggagaaga
      601 acttttcact ggagttgtcc caattttagt tgaactagat ggcgacgtga acggtcataa
      661 gttcagtgtc tccggcgaag gtgagggtga tgcaacgtac ggtaagttaa ctttgaagtt
      721 aatatgtaca accggcaagc tgcctgttcc ctggcctacc ctggtgacaa cgttaggtta
      781 tgggttgatg tgctttgcta gatacccaga tcacatgaaa aggcatgact tctttaaatc
      841 tgcaatgcca gaaggttacg tccaagaacg tactattttc tttaaagatg acggtaatta
      901 taaaactagg gctgaagtta aattcgaagg tgacacactt gtaaatcgaa tagagttaaa
      961 ggggattgat ttcaaagagg atggtaatat tctaggccat aaacttgaat ataactataa
     1021 ttcacacaac gtttacatta ccgccgacaa gcagaagaat ggaatcaaag ccaattttaa
     1081 gattagacac aatattgagg atggtggagt acagcttgct gatcattacc aacaaaatac
     1141 cccgatcggt gatggaccag ttttgctacc cgataaccat tatctgtcct atcaaagcaa
     1201 attgtcaaaa gatcctaacg aaaaaagaga ccacatggta ctcttggaat ttgtaacagc
     1261 tgctgggatt acacatggca tggatgaact atacaaaggt tctggtaccg cagcatgatg
     1321 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1381 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1441 ggcctttctg cgtttatata ctagtagcgg ccgctgcagg agtcactaag ggttagttag
     1501 ttagattagc agaaagtcaa aagcctccga ccggaggctt ttgactaaaa cttcccttgg
     1561 ggttatcatt ggggctcact caaaggcggt aatcagataa aaaaaatcct tagctttcgc
     1621 taaggatgat ttctgctaga gctgtcagac caagtttacg agctcgcttg gactcctgtt
     1681 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
     1741 ccgggcgttt tttattggtg agaatccaag cactagggac agtaagacgg gtaagcctgt
     1801 tgatgatacc gctgccttac tgggtgcatt agccagtctg aatgacctgt cacgggataa
     1861 tccgaagtgg tcagactgga aaatcagagg gcaggaactg ctgaacagca aaaagtcaga
     1921 tagcaccaca tagcagaccc gccataaaac gccctgagaa gcccgtgacg ggcttttctt
     1981 gtattatggg tagtttcctt gcatgaatcc ataaaaggcg cctgtagtgc catttacccc
     2041 cattcactgc cagagccgtg agcgcagcga actgaatgtc acgaaaaaga cagcgactca
     2101 ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg
     2161 ctacttaagc ctttagggtt ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac
     2221 atccttttgt aatactgcgg aactgactaa agtagtgagt tatacacagg gctgggatct
     2281 attcttttta tcttttttta ttctttcttt attctataaa ttataaccac ttgaatataa
     2341 acaaaaaaaa cacacaaagg tctagcggaa tttacagagg gtctagcaga atttacaagt
     2401 tttccagcaa aggtctagca gaatttacag atacccacaa ctcaaaggaa aaggacatgt
     2461 aattatcatt gactagccca tctcaattgg tatagtgatt aaaatcacct agaccaattg
     2521 agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac tagcgattag tcgctatgac
     2581 ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg cactactcaa ccccacgatt
     2641 gaaaacccta caaggaaaga acggacggta tcgttcactt ataaccaata cgctcagatg
     2701 atgaacatca gtagggaaaa tgcttatggt gtattagcta aagcaaccag agagctgatg
     2761 acgagaactg tggaaatcag gaatcctttg gttaaaggct ttgagatttt ccagtggaca
     2821 aactatgcca agttctcaag cgaaaaatta gaattagttt ttagtgaaga gatattgcct
     2881 tatcttttcc agttaaaaaa attcataaaa tataatctgg aacatgttaa gtcttttgaa
     2941 aacaaatact ctatgaggat ttatgagtgg ttattaaaag aactaacaca aaagaaaact
     3001 cacaaggcaa atatagagat tagccttgat gaatttaagt tcatgttaat gcttgaaaat
     3061 aactaccatg agtttaaaag gcttaaccaa tgggttttga aaccaataag taaagattta
     3121 aacacttaca gcaatatgaa attggtggtt gataagcgag gccgcccgac tgatacgttg
     3181 attttccaag ttgaactaga tagacaaatg gatctcgtaa ccgaacttga gaacaaccag
     3241 ataaaaatga atggtgacaa aataccaaca accattacat cagattccta cctacgtaac
     3301 ggactaagaa aaacactaca cgatgcttta actgcaaaaa ttcagctcac cagttttgag
     3361 gcaaaatttt tgagtgacat gcaaagtaag catgatctca atggttcgtt ctcatggctc
     3421 acgcaaaaac aacgaaccac actagagaac atactggcta aatacggaag gatctgaggt
     3481 tcttatggct cttgtatcta tcagtgaagc atcaagacta acaaacaaaa gtagaacaac
     3541 tgttcaccgt tagatatcaa agggaaaact gtccatatgc acagatgaaa acggtgtaaa
     3601 aaagatagat acatcagagc ttttacgagt ttttggtgca tttaaagctg ttcaccatga
     3661 acagatcgac aatgtaacta ctagagctaa atacattcaa atatctatcc gctcatgaga
     3721 caataaccct gataaatgct tcaataatat tgaaaaagga agaatatgag tattcaacat
     3781 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3841 gaaacgctgg tgaaagtaaa agatgccgaa gatcagttgg gtgcacgtgt gggttacatc
     3901 gaactggacc tcaacagcgg taagattctt gagagttttc gccccgaaga acgtttccca
     3961 atgatgagca cttttaaagt tctgctctgt ggcgcggtat tatcccgtat tgacgccggg
     4021 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4081 gtcacagaaa agcatcttac ggacggcatg acagtacgcg aattatgcag cgctgccata
     4141 accatgagtg ataacacggc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4201 cttaccgctt ttttgcacaa catgggtgat catgtaactc gccttgatcg ttgggaaccg
     4261 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agctatggca
     4321 acaacgttgc gcaaactctt aactggcgaa cttcttactc tcgcttcccg gcaacaatta
     4381 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4441 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtcccgcgg tattattgca
     4501 gccctggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagccag
     4561 gcaactatgg acgaacgtaa tcgccagatc gctgagatag gtgcctccct gattaagcat
     4621 tggtaataat actagctccg gcaaaaaaac gggcaa
//
