LOCUS       pBEL2665_(MlaADelta244- 5290 bp ds-DNA     circular     28-NOV-2022
DEFINITION  .
KEYWORDS    "Key Features:MlaA (Delta244-251), Arabinose-inducible; without the
            second helix in the C-terminal extension" "Backbone:pBE1194"
            "Construction:Gibson cloning of MlaA gene amplified from pBEL1242
            into pBE1194 vector cut with KpnI and NheI" "Resistance:Carb"
            "Location" "Rack" "Box" "Notes:Used for MlaA complementation assays
            (Mark and Dhenesh common project)" "Sequencing information"
            "Special Category"
COMMENT     Imported using the Genbank importer. File name: pBE1194.ape
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        13..285
                     /label="araBAD promoter region"
     primer          133..161
                     /label="DPF42"
                     /note="sequence: CCATGACAAAAACGCGTAACAAAAGTGTC"
     primer          217..236
                     /label="pBAD Forward"
                     /note="sequence: ATGCCATAGCATTTTTATCC"
     primer          219..248
                     /label="DPF41"
                     /note="sequence: GCCATAGCATTTTTATCCATAAGATTAGCG"
     primer          219..248
                     /label="oDCE3179"
                     /note="sequence: GCCATAGCATTTTTATCCATAAGATTAGCG"
     primer          275..334
                     /label="DPF38"
                     /note="sequence: ACTCTCTACTGTTTCTCCATACCCGTTTTTTTGGGCTAGCatgaagcttcgcctgtcggc"
     CDS             313..314
                     /label="orf1"
     misc_feature    315..365
                     /label="signal peptide (?)"
     CDS             315..1043
                     /label="MlaA, phospholipid-binding lipoprotein"
     CDS             315..1043
                     /label="Translation 315-1043"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRDDGGDMADGFYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQAIQD"
     misc_feature    366..368
                     /label="Acylated Cys residue?"
     primer          complement(1021..1080)
                     /label="DPR54bis"
                     /note="sequence: GCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCttaatcctgaatcgcttgtgcgttcg"
     misc_feature    1044..1046
                     /label="Stop codon"
     CDS             1044..1046
                     /label="Translation 1044-1046"
                     /translation="*"
     primer          1096..1120
                     /label="oDCE3221"
                     /note="sequence: GGTCCTGGTCTGAGGAATGGTTGTA"
     primer          complement(1121..1141)
                     /label="oDCE3180"
                     /note="sequence: ATCCGCCAAAACAGCCAAGCT"
     primer          complement(1177..1206)
                     /label="DPR41"
                     /note="sequence: ATTCTGTTTTATCAGACCGCTTCTGCGTTC"
     terminator      1208..1365
                     /label="rrnB transcription terminator"
     primer          complement(1243..1267)
                     /label="DPR42"
                     /note="sequence: GTTTCACTTCTGAGTTCGGCATGGG"
     misc_feature    1344..1368
                     /label="BE2467"
     primer          1344..1368
                     /label="oDCE3217"
                     /note="sequence: CTCAGTCGAAAGACTGGGCCTTTCG"
     CDS             1644..2504
                     /label="Ampicillin ORF"
     misc_feature    2188..2188
                     /label="SeqResult T->C"
     rep_origin      3036..3717
                     /label="ColE1 origin"
     misc_feature    4092..4092
                     /label="SeqResC->A"
     misc_feature    4095..4097
                     /label="SeqResATC->GAG"
     misc_feature    4099..4303
                     /label="SeqRes 205bp insert"
     CDS             complement(4395..5282)
                     /label="araC"
ORIGIN
        1 CATTCAGAGA AGAAACCAAT TGTCCATATT GCATCAGACA TTGCCGTCAC TGCGTCTTTT
       61 ACTGGCTCTT CTCGCTAACC AAACCGGTAA CCCCGCTTAT TAAAAGCATT CTGTAACAAA
      121 GCGGGACCAA AGCCATGACA AAAACGCGTA ACAAAAGTGT CTATAATCAC GGCAGAAAAG
      181 TCCACATTGA TTATTTGCAC GGCGTCACAC TTTGCTATGC CATAGCATTT TTATCCATAA
      241 GATTAGCGGA TCCTACCTGA CGCTTTTTAT CGCAACTCTC TACTGTTTCT CCATACCCGT
      301 TTTTTTGGGC TAGCatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
      361 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
      421 accgcaccat gtacaacttc aacttcaatg tattagaccc gtatattgtt cgaccggtcg
      481 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa cggtttgagc aactttactg
      541 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
      601 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggttttattg
      661 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
      721 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
      781 tcacgctgcg tgatgacggt ggtgatatgg cggatggttt ttacccggtt ctttcctggc
      841 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
      901 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
      961 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     1021 cgaacgcaca agcgattcag gattaaGGTA CCAAATTCCA GAAAAGAGAC GCTGAAAAGC
     1081 GTCTTTTTTC GTTTTGGTCC TGGTCTGAGG AATGGTTGTA AGCTTGGCTG TTTTGGCGGA
     1141 TGAGAGAAGA TTTTCAGCCT GATACAGATT AAATCAGAAC GCAGAAGCGG TCTGATAAAA
     1201 CAGAATTTGC CTGGCGGCAG TAGCGCGGTG GTCCCACCTG ACCCCATGCC GAACTCAGAA
     1261 GTGAAACGCC GTAGCGCCGA TGGTAGTGTG GGGTCTCCCC ATGCGAGAGT AGGGAACTGC
     1321 CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG
     1381 TTTGTCGGTG AACGCTCTCC TGAGTAGGAC AAATCCGCCG GGAGCGGATT TGAACGTTGC
     1441 GAAGCAACGG CCCGGAGGGT GGCGGGCAGG ACGCCCGCCA TAAACTGCCA GGCATCAAAT
     1501 TAAGCAGAAG GCCATCCTGA CGGATGGCCT TTTTGCGTTT CTACAAACTC TTTTGTTTAT
     1561 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC
     1621 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT
     1681 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG
     1741 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA
     1801 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC
     1861 TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA
     1921 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG
     1981 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG
     2041 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA
     2101 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA
     2161 ACGACGAGCG TGACACCACG ATGCCTGcAG CAATGGCAAC AACGTTGCGC AAACTATTAA
     2221 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA
     2281 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT
     2341 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC
     2401 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA
     2461 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT
     2521 ACTCATATAT ACTTTAGATT GATTTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT
     2581 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
     2641 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG
     2701 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT
     2761 TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG
     2821 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT
     2881 ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
     2941 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT
     3001 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA
     3061 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     3121 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     3181 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT CAGCAGAGCG
     3241 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT
     3301 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC
     3361 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG
     3421 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
     3481 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
     3541 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG
     3601 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA
     3661 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT
     3721 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT
     3781 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA
     3841 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT
     3901 CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATAGGGT CATGGCTGCG CCCCGACACC
     3961 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC
     4021 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC
     4081 GCGCGAGGCA GaAGgagATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC
     4141 CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA
     4201 TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC
     4261 GTCCGGCGTA GAGGATCTGC TCATGTTTGA CAGCTTATCA TCGATGCATA ATGTGCCTGT
     4321 CAAATGGACG AAGCAGGGAT TCTGCAAACC CTATGCTACT CCGTCAAGCC GTCAATTGTC
     4381 TGATTCGTTA CCAATTATGA CAACTTGACG GCTACATCAT TCACTTTTTC TTCACAACCG
     4441 GCACGGAACT CGCTCGGGCT GGCCCCGGTG CATTTTTTAA ATACCCGCGA GAAATAGAGT
     4501 TGATCGTCAA AACCAACATT GCGACCGACG GTGGCGATAG GCATCCGGGT GGTGCTCAAA
     4561 AGCAGCTTCG CCTGGCTGAT ACGTTGGTCC TCGCGCCAGC TTAAGACGCT AATCCCTAAC
     4621 TGCTGGCGGA AAAGATGTGA CAGACGCGAC GGCGACAAGC AAACATGCTG TGCGACGCTG
     4681 GCGATATCAA AATTGCTGTC TGCCAGGTGA TCGCTGATGT ACTGACAAGC CTCGCGTACC
     4741 CGATTATCCA TCGGTGGATG GAGCGACTCG TTAATCGCTT CCATGCGCCG CAGTAACAAT
     4801 TGCTCAAGCA GATTTATCGC CAGCAGCTCC GAATAGCGCC CTTCCCCTTG CCCGGCGTTA
     4861 ATGATTTGCC CAAACAGGTC GCTGAAATGC GGCTGGTGCG CTTCATCCGG GCGAAAGAAC
     4921 CCCGTATTGG CAAATATTGA CGGCCAGTTA AGCCATTCAT GCCAGTAGGC GCGCGGACGA
     4981 AAGTAAACCC ACTGGTGATA CCATTCGCGA GCCTCCGGAT GACGACCGTA GTGATGAATC
     5041 TCTCCTGGCG GGAACAGCAA AATATCACCC GGTCGGCAAA CAAATTCTCG TCCCTGATTT
     5101 TTCACCACCC CCTGACCGCG AATGGTGAGA TTGAGAATAT AACCTTTCAT TCCCAGCGGT
     5161 CGGTCGATAA AAAAATCGAG ATAACCGTTG GCCTCAATCG GCGTTAAACC CGCCACCAGA
     5221 TGGGCATTAA ACGAGTATCC CGGCAGCAGG GGATCATTTT GCGCTTCAGC CATACTTTTC
     5281 ATACTCCCGC
//