LOCUS       pUC57mini_v2-HF24_F1_        1971 bp ds-DNA     circular SYN 12-DEC-2022
DEFINITION  Fragment#1 of a 24-fragment split in lacI/lacZ cassette.
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Na Ke
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2019 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            9..144
                     /standard_name="HF24_F1"
     rep_origin      complement(216..804)
     CDS             complement(975..1835)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="AmpR"
     regulatory      complement(1836..1940)
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 ccaatgatgg tctcgggagt catttttgac accagaccaa ctggtaatgg tagcgaccgg
       61 cgctcagctg gaattccgcc gatactgacg ggctccagga gtcgtcgcca ccaatcccca
      121 tatggaaacc gtcgatacga gaccatcgga aagaacatgt gagcaaaagg ccagcaaaag
      181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      961 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1081 ttaccatctg gccccagtgc tgcaatgata ccgcgagatc cacgctcacc ggctccagat
     1141 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1261 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1501 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1561 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1801 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
